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Srinagar, India
Srinagar, India
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Kumar P.,Directorate of ResearchUniversity of Horticultural science | Dolkar R.,SKUAST | Manjunatha G.,Directorate of ResearchUniversity of Horticultural science
South African Journal of Botany | Year: 2017

Moringa oleifera L. is one of the important vegetable crop having huge commercial and nutraceutical value. Understanding the genetic diversity among advanced breeding lines is prerequisite to any genetic improvement and development of superior cultivars. In the current study, genetic variation and relationships among seven advanced breeding lines were investigated using seed protein profile, 31 RAPD and 10 Cytochrome P450 gene based markers. Seed protein exhibited variation in breeding lines G1 and G5 by lacking the protein bands of 21 kDa and 35 kDa. Selected RAPD and Cytochrome P450 gene markers showed an average of 79.68 and 86.44% polymorphism respectively. Marker index RAPD (1.65), Cytochrome P450 (4.81) suggests that Cytochrome P450 gene markers are most efficient for variability analysis. Dendrogram constructed for genetic similarity using UPGMA clustered genotypes into two main clusters and two sub-clusters, indicates variation within the lines and a major cultivar. High bootstrap values (100 and 85) were obtained at major nodes of the dendrogram by means of WINBOOT software. Principal coordinate analysis plots of the two markers were found highly concordant with each other. Mantel test was employed to determine cophenetic correlation and value above 0.90 for both marker systems was obtained. Distinct bands observed in KDM-01 and Dhanraj can be utilized for marker assisted selection. Comparing these functional and non-functional markers suggested that these marker systems are promising in assessment of genetic diversity among advance breeding lines of Moringa oleifera L. and could be exploited for breeding program, DNA fingerprinting, marker assisted selection and cultivar development. © 2017 SAAB


Iqbal A.,SKUAST | Ahmad M.,Private Field Veterinarian
Journal of Pure and Applied Microbiology | Year: 2012

The present study describes the prevalence of Bacillus cereus isolated from mutton tikka and chutney samples in different seasons in Kashmir valley. A total of 100 street vended food samples comprising of 60 mutton tikka and 40 chutney samples were tested. Bacillus cereus strains were isolated from 27 of the mutton tikka and 13 of the chutney samples resulting in overall prevalence of 45% and 32.5%, respectively. The field isolates and the standard strains of Bacillus cereus had similar cultural, morphological and biochemical characteristics.


Sharma S.,SKUAST | Kumar K.,UHF | Kumar A.,SKUAST KashmirJammu and Kashmir
Advances in Horticultural Science | Year: 2014

Genetic divergence of 29 exotic walnut cultivars introduced from abroad and 25 local selections from seedling population was studied. The analysis of variance revealed significant differences among accessions for each character under study. Based on Mahalanobis D2 values the accessions were grouped into eight clusters. Cluster I had a maximum of 12 accessions followed by cluster II with 11 accessions. The mean intra and inter cluster distance (D) revealed that cluster VII had the highest intra cluster distance (2.171), while the inter cluster distance was the greatest between cluster II and IV (10.528). Characters like plant height, trunk girth, plant spread, plant volume, leaf length, leaf width and number of leaflets contributed the most to total divergence. © 2014, University of Florence. All rights reserved.


Mir S.H.,University Of Kashmir | Darzi M.M.,SKUAST | Mir M.S.,SKUAST
Iranian Journal of Pathology | Year: 2013

Background and Objectives: Medicinal plants are well documented for possessing antidiabetic or antihyperglycaemic potential. Abroma augusta seems to have the same effect in treating diabetes mellitus. The aim of the present experiment was to report the efficacy of the aqueous extract of the leaves of A. augusta on biochemical and histological abnormalities of alloxan-induced diabetic rabbits. Materials and Methods: An experiment was conducted to study the effect of aqueous extract of the leaves of A. augusta on biochemical values and histomorphological features of pancreas and kidneys of alloxan-induced (@ 80 mg/kg. body weight intraperitoneally) diabetic rabbits. The animals were divided into three groups viz, normal control, diabetic control and A. augusta treated diabetic rabbits. The extract was given by gavages at a dose of 2ml/kg body weight twice daily for a period of 21 days. Result: Normal blood sugar (P<0.01), blood urea (P<0.01) and serum creatinine (P<0.02) levels were observed. Histological examination of the pancreas showed an increase in the number of beta cells in the A. augusta treated diabetic rabbits. Further, the extract was found to ameliorate the histological abnormalities of the kidneys in diabetic rabbits. Conclusion: This experimental study indicates that A. augusta extract possess either antioxidant effect or regenerative ability in ameliorating the biochemical and histomorphological abnormalities in diabetic rabbits.


Kumar A.,Indian Agricultural Research Institute | Solanki I.S.,ADG F and FC ICAR | Akhtar J.,ICAR | Gupta V.,SKUAST
Journal of Pure and Applied Microbiology | Year: 2016

Brown spot of paddy is prevalent in all the rice growing countries of the world and most of the cultivars grown are susceptible to this pathogen. Though the disease is considered to be minor one, it is increasingly posing a serious emerging threat. A field experiment on management of brown spot of paddy was carried out in two consecutive Kharif seasons (2012, 2013) on two aromatic rice varieties Pusa Sugandh-4 and Pusa Sugandh-5 in hot spot area for brown spot disease at IARI Regional Station Pusa (Bihar). Eleven different treatment combinations involving combination of fungicides and bioagent were used in this experiment. The approach of seed treatment, seedling dip and foliar spray alone and in combination was tried against brown spot of paddy. The disease data was recorded in three stages for disease rating using SES of IRRI. T8, Seed treatment with Carboxin 37.5% and Thiram 37.5% WS @2.5 gm kg-1 seed and Seedling dip in suspension of Pseudomonas flourescens @ 10gm/ litre followed by two sprays of Propiconazole 25% EC @0.1% at 45 days and 60 days after transplanting gave best result. Minimum disease severity and maximum yield was observed under this treatment combination. As far as only seed treatment is concerned a fungicidal combination of Carboxin 37.5% and Thiram 37.5% WS @2.5 gm kg-1 seed was found superior than other seed treated plots. An yield advantage of 13.6 q/ha in Pusa sugandh-4 and 22 q/ha in Pusa Sugandh-5 has been observed over control. The increased incidence of this disease in recent years and lack of information about suitable management strategies based on field observations are the key drivers in formulation of this experiment.


Sharma S.,UHF | Kumar K.,UHF | Kumar A.,SKUAST
Journal of Applied Horticulture | Year: 2015

Exotic cultivars introduced from abroad and local selections from seedling population of walnut were subjected to Non-hierarchical Euclidean cluster analysis based on 12 nut and kernel characters. Studied accessions were grouped into four clusters, where cluster 1 and 3 had majority of the accessions. The clustering pattern of walnut genotypes belonging to the same eco-geographical region revealed their distribution in more than one cluster showing between geographic and genetic diversity. The first component presented maximum eigen root value and per cent variation. The mean intra and inter cluster distance (D) revealed that cluster 4 had highest intra cluster distance (2.206), while the inter cluster distance was maximum between cluster 3 and 4 (5.806). Maximum mean nut yield, nut length, nut weight and kernel weight was recorded in cluster 4, whereas, maximum kernel percentage and fat percentage was recorded in cluster 1 and 2, respectively. Minimum shell thickness was observed in cluster 2. The accessions of cluster 3 and 4 were found highly diverse from each other and will give better segregants after hybridization which can be used as a parent in further breeding programme.


Gupta R.D.,SKUAST | Arora S.,SKUAST J | Gupta G.D.,Soil Survey Land Use Planning | Sumberia N.M.,SKUAST J
Tropical Ecology | Year: 2010

Physical properties of some soils from foothills of Siwaliks of Jammu, Jammu and Kashmir State were studied. Clay content was the highest in soils of forest profiles, followed by those under cultivated unmanaged and well-managed profiles, and least in soils of barren land profiles. Soils of barren lands showed the highest values of bulk density (1.47 to 1.60 g cm-3), followed by cultivated unmanaged (1.46 to 1.58 g cm-3), cultivated well-managed lands (1.34 to 1.54 g cm-3), and least in those of forest lands (1.32 to 1.52 g cm-3). The values of particle density decreased in the order: barren lands (2.57 to 2.68 g cm-3) > cultivated unmanaged (2.52 to 2.67 g cm-3) > cultivated well-managed (2.44 to 2.62 g cm-3) > forest lands (2.38 to 2.62 g cm-3). Soils of forest lands had generally higher values of pore space (41.4 to 47.2 %) followed by cultivated well-managed lands (41.4 to 45.1%), barren (40.1 to 43.4%) and unmanaged cultivated lands (40.0 to 43.2%). Water holding capacity (%) ranged from 21.9 to 32.2, 30.5 to 40.5, 35.4 to 47.5 and 35.3 to 47.3 in soils of barren, cultivated unmanaged, cultivated well managed and forest lands, respectively. Soils of forest lands showed highest values of moisture equivalent (23.4%) and lowest in those of barren lands (15.7%), while there was not much difference in the moisture equivalent values of soils belonging to cultivated unmanaged (21.5%) and cultivated well-managed (22.9%) lands. Erosion and dispersion ratio were positively and significantly correlated with particle and bulk density. Water holding capacity and moisture equivalents were positively related to organic carbon content, and negatively related to erosion and dispersion ratio. © International Society for Tropical Ecology.


Asif M.,SKUAST | Lone S.,University of Pune | Lone F.A.,Government Degree College | Hamid A.,SKUAST
International Journal of Pharma and Bio Sciences | Year: 2013

A pot experiment was carried out during 2009 and 2010 to study the field performance of blue pine seedlings inoculated with selected species of bio-inoculants under nursery conditions . The experiment was laid in Completely Randomized Design with three replications which comprised of seven inoculants (Azotobacter sp., Azospirillum sp., Pseudomonas fluorescens, Bacillus subtilis, Pisolithus tinctorius, Laccaria laccata and control). Various growth characters viz., shoot height, collar diameter, root length, and seedling survival at various intervals responded significantly to all the microbial inoculants. Among microbial inoculants the two mycorrhizae viz., Pisolithus tinctorius and Laccaria laccata proved beneficial for all growth parameters than rest of the inoculants. It was followed by Azotobacter sp., Azospirillum sp., Pseudomonas fluorescens and Bacillus subtilis. However for root length Pseudomonas fluorescens and Bacillus subtilis gave best results than Azotobacter sp. and Azospirillum sp. Microbial inoculation of Pisolithus tinctorius and Laccaria laccata gave best results with respect to per cent decrease in seedling mortality rate of the species. Thus the two treatments viz; Pisolithus tinctorius and Laccaria laccata proved superior for all the studied growth parameters. Our findings show that the application of bio-inoculants improve the growth attributes of blue pine seedlings under natural field conditions.


Asif M.,Skuast | Zargar M.Y.,Regional Research Station
International Journal of Pharma and Bio Sciences | Year: 2013

This study was carried out at the forest nursery of Department of Forestry, Faculty of Agriculture and Regional Research Station, Wadura SKUAST-K, Sopore, Kashmir during 2006 and 2007 to examine the role of live biofertilizers in litter decomposition and nutrient release in Salix fragilis under natural Salix stands. The experiment was laid in completely randomised design with three replications which comprised five treatment combinations of 5 inoculants (no-inoculant; Azotobacter, Chroococcum, Pseudomonas fluorescens, effective microorganisms and combinations of Azotobacter chroococcum + Pseudomonas fluorescens + effective microorganisms). Higher rate of decomposition of Salix fragilis litter was recorded in June (89.29%). Lower rate of decomposition of the species was recorded in January (39.07%). Plant N, P, K, Ca, and Mg release showed an increasing trend from July onwards upto November and immobilization of above nutrients was observed in December and January. However, in the succeeding months an increasing trend in the nutrient release was observed. Highest nutrient release was recorded under combined inoculation of Azotobacter chrococcum + Psedomonas fluorescens + effective microorganisms followed by effective microorganims as compared to other treatments and control. Combined biofertilizer inoculation resulted in a significant increase in total viable bacteria, fungi and actinomycetes followed by effective microorganisms, Pseudomonas fluorescens, Azotobacter chroococcum and control respectively. Thus the treatment combination of Azotobacter ch ococcum + Pseudomonas fluerescens + effective microorganisms proved to be the best for decomposition of Salix fragilis litter and nutrient release.


Padder S.A.,SKUAST | Dar G.H.,SKUAST | Mohiddin F.A.,SKUAST | Shah M.D.,SKUAST
Journal of Pure and Applied Microbiology | Year: 2016

The present study was conducted to reconnoiter the potential of endophytic bacterium of brown sarson root tissues, Pseudomonas fluorescens strain smppsap5 for enhancing the growth of brown sarson plants under organic agriculture of Jammu and Kashmir, India. The novel bacterium strain smppsap5 isolated was identified as Pseudomonas fluorescens using 16S ribosomal RNA gene sequencing. The isolated strain was selected among 81 isolates for highest phosphate solubilization activity in terms of the phosphate solubilization index (3.19) and phosphate released (313.58 mg/L) from tri-calcium phosphate in the liquid assay. The optimal phosphate solubilizing activity of the strain was determined with different incubation periods and nitrogen sources. It was observed that maximum phosphate solubilization occurred in presence of the nitrogen source tryptone and incubation period of 72h and phosphate solubilization was minimum among all with nitrogen source NaNO3 and incubation period of 24h. The effect of temperature and PEG on growth of this strain was also observed besides many metabolic, physiological and biochemical attributes. The beneficial effect of Pseudomonas fluorescens strain smppsap5 in brown sarson plants was observed in terms of root shoot length, germination percentage, chlorophyll content, oil content and other yield attributes besides its inoculation impact on soil physio-chemical and biological properties. The results from the present study suggested that Pseudomonas fluorescens strain smppsap5 enhanced the plant and soil health when compared to control and thus it could be used as a potential bio inoculant under integrated, organic and sustainable farming systems.

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