Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology

Moscow, Russia

Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology

Moscow, Russia
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Bukharina N.S.,RAS Institute of Chemistry | Pleshakova T.O.,RAS Institute of Chemistry | Ziborov V.S.,RAS Joint Institute for High Temperatures | Fokin D.A.,Bruker | And 2 more authors.
Science of Advanced Materials | Year: 2017

Deformation of single cytochrome CYP102A1 (P450 BM3) molecules and its influence on protein fluctuation during the catalytic cycle were explored by atomic force microscopy (AFM). A variety of AFM modes allowed to study CYP102A1 elasticity. Measurements were carried out in three AFM modes: force spectroscopy (FS mode), PeakForce QNM and tapping modes. AFM measurements in a tapping mode were conducted using standard probes (the resonance frequency of about 9 kHz in liquid) and short high-speed probes (the resonance frequency of about 850 kHz in liquid). It was shown that the relationship of a loading rate and protein deformation was nonlinear. CYP102A1 Young's modulus of 1.3-35 MPa was estimated from AFM force- distance curves. CYP102A1 deformation in a tapping mode with high-speed probes was greater compared to that in a tapping mode with standard probes. Increasing of deformation was associated with a disappearance of CYP102A1 fluctuations during AFM monitoring of enzyme catalytic cycle. © 2017 by American Scientific Publishers.


Gordeeva Y.L.,Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology | Rudakovskaya E.G.,Mendeleev University of Chemical Technology | Gordeeva E.L.,Mendeleev University of Chemical Technology | Borodkin A.G.,Mendeleev University of Chemical Technology
Theoretical Foundations of Chemical Engineering | Year: 2017

The results of analyzing mathematical models of the process of batch fermentation for the production of lactic acid have been presented. Three groups of mathematical models have been considered. The first two groups are based exclusively on the notion of the specific growth rate, dependent or independent on substrate concentration. The third group uses the notions of the specific rate of substrate consumption and specific rate of product formation in additions to the notion of specific growth rate. The numeric estimates of constants are presented for the majority of models as well as comparison with the experimental studies. Mathematical models of the processes where the formation of side products is possible have been discussed, which may or may not be of value on their own. The possible practical applicability of the models has been evaluated based on the results of the analysis. © 2017, Pleiades Publishing, Ltd.


Zaitsev S.Y.,Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
Moscow University Chemistry Bulletin | Year: 2017

Measurements of the dynamic surface tension (DST) of liquids is one of the simplest and fastest methods for the analysis of various chemical solutions and biological liquids. Solutions of the three-component systems on the basis of bovine serum albumin (BSA), natural phosphatidylcholine (PC), and sodium chloride are investigated. It is found that the salt content (especially at high concentrations) exerts a major impact during short time periods (0.1–1.0 s); and the BSA concentration, in intermediate and long time periods (over 1.0 s); however, changes in the PC concentration (in the range of concentrations typical for blood) insignificantly affect most of the DST parameters. The changes (by 22–68%) of the tensiogram slope values, λ0, λ1, and λ2, are most informative among the seven studied DST parameters. This has allowed us to determine the optimal concentrations ranges for BSA (60–80 g/L), PC (1–4 mM), and NaCl (130–150 mM) in the mixture, which correspond to their content in the blood plasma of humans and animals. For four parameters, σ1…σ4, only small correlations with changes in the proportion of the three components in the mixture are found. The obtained relationships are important for both supplementing fundamental databases and prognostic modeling in biology and medicine. © 2017, Allerton Press, Inc.


Speranskaya A.S.,RAS Engelhardt Institute of Molecular Biology | Krinitsina A.A.,Moscow State University | Kudryavtseva A.V.,RAS Engelhardt Institute of Molecular Biology | Poltronieri P.,CNR Institute of Sciences of Food Production | And 6 more authors.
Biochimie | Year: 2012

Background: The group of Kunitz-type protease inhibitors (KPI) from potato is encoded by a polymorphic family of multiple allelic and non-allelic genes. The previous explanations of the KPI variability were based on the hypothesis of random mutagenesis as a key factor of KPI polymorphism. Results: KPI-A genes from the genomes of Solanum tuberosum cv. Istrinskii and the wild species Solanum palustre were amplified by PCR with subsequent cloning in plasmids. True KPI sequences were derived from comparison of the cloned copies. "Hot spots" of recombination in KPI genes were independently identified by DnaSP 4.0 and TOPALi v2.5 software. The KPI-A sequence from potato cv. Istrinskii was found to be 100% identical to the gene from Solanum nigrum. This fact illustrates a high degree of similarity of KPI genes in the genus Solanum. Pairwise comparison of KPI A and B genes unambiguously showed a non-uniform extent of polymorphism at different nt positions. Moreover, the occurrence of substitutions was not random along the strand. Taken together, these facts contradict the traditional hypothesis of random mutagenesis as a principal source of KPI gene polymorphism. The experimentally found mosaic structure of KPI genes in both plants studied is consistent with the hypothesis suggesting recombination of ancestral genes. The same mechanism was proposed earlier for other resistance-conferring genes in the nightshade family (Solanaceae). Based on the data obtained, we searched for potential motifs of site-specific binding with plant DNA recombinases. During this work, we analyzed the sequencing data reported by the Potato Genome Sequencing Consortium (PGSC), 2011 and found considerable inconsistence of their data concerning the number, location, and orientation of KPI genes of groups A and B. Conclusions: The key role of recombination rather than random point mutagenesis in KPI polymorphism was demonstrated for the first time. © 2012 Elsevier Masson SAS. All rights reserved.


Ivanov Y.D.,RAS Institute of Chemistry | Kozlov A.F.,RAS Institute of Chemistry | Malsagova K.A.,RAS Institute of Chemistry | Pleshakova T.O.,RAS Institute of Chemistry | And 4 more authors.
Biochemistry and Biophysics Reports | Year: 2016

Monitoring of microwave emission from aqueous solution of horseradish peroxidase (HRP) in the process of the enzyme functioning was carried out. For the monitoring, a system containing HRP, luminol and n2O2 was employed. Microwave emission measurements were carried out in the 3.4-4.2 GHz frequency range using the active and passive modes (active-mode and passive-mode measurements). In the active mode, excitation of the solution in the pulsed electromagnetic field was accomplished. In the passive mode, no excitation was induced. It appears that the passive-mode measurements taken in the course of the peroxidase reaction in the enzyme system have shown a 0.5 °s increase of the microwave signal. Upon the active-mode measurements, taken in the same reaction conditions, the forced excitation of the solution has also led to the increase (by 2 °s) of the level of the microwave signal - i.e. to its 4-fold enhancement compared to the signal obtained in passive-mode measurements. © 2016 The Authors.


Guseva M.A.,Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology | Epova E.Y.,Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology | Kovalev L.I.,RAS A.N. Bach Institute of Biochemistry | Shevelev A.B.,RAS A.N. Bach Institute of Biochemistry
Applied Biochemistry and Microbiology | Year: 2010

Using proteomic technologies-two-dimensional electrophoresis in denaturing conditions in combination with mass spectroscopy of MALDI-TOF proteins-we demonstrated, for the first time, that the most noticeable alteration of protein composition of a Yarrowia lipolytica cell during adaptation to alkaline conditions was an increase of mitochondrial proteins relatively to proteins of cytoplasm. © Pleiades Publishing, Inc., 2010.


Zaitseva V.V.,Ukrainian Academy of Sciences | Zaitseva V.V.,Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology | Tyurina T.G.,Ukrainian Academy of Sciences | Zaitsev S.Y.,Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
Russian Journal of Organic Chemistry | Year: 2015

Schemes of complex formation and decomposition in monomers copolymerization in the absence of initiator (313 K) are suggested. The energy of formation of π-H- and H-complexes of N-vinylpyrrolidone with maleic anhydride, methyl methacrylate, or two latter with each other is 9.9-18.0 kJ mol-1, the equilibrium constant is 0.58-0.19 L mol-1 (CCl4). In the case of N-vinylpyrrolidone complexes with maleic anhydride we found the proceeding of a parallel radical process of formation of dimers, oligomers, and copolymers till ~10 wt % conversion. The copolymerization rate of these monomers is higher than the rate of their homopolymerization. © 2015 Pleiades Publishing, Ltd.


Starodubova E.S.,RAS Engelhardt Institute of Molecular Biology | Preobrazhenskaia O.V.,RAS Engelhardt Institute of Molecular Biology | Kuzmenko Y.V.,RAS Engelhardt Institute of Molecular Biology | Latanova A.A.,RAS Engelhardt Institute of Molecular Biology | And 2 more authors.
Molecular Biology | Year: 2015

Rabies is an infectious disease among humans and animals that remains incurable, despite its longstanding research history. The only way to prevent the disease is prompt treatment, including vaccination as an obligatory component and administration of antirabies immunoglobulin as a supplement. Since the first antirabies vaccination performed in the 19th century, a large number of different rabies vaccines have been developed. Progress in molecular biology and biotechnology enabled the development of effective and safe technologies of vaccine production. Currently, new-generation vaccines are being developed based on recombinant rabies virus strains or on the production of an individual recombinant rabies antigen—glycoprotein (G protein), either as a component of nonpathogenic viruses, or in plants, or in the form of DNA vaccines. In this review, the main modern trends in the development of rabies vaccines have been discussed. © 2015, Pleiades Publishing, Inc.


PubMed | Moscow State University and Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
Type: Journal Article | Journal: Biomeditsinskaia khimiia | Year: 2016

Perioperative corneal abrasion is an ophthalmic complication commonly found in patients underwent general anesthesia. In this study, correlations between development of corneal injury and proteomic changes in tear film during general anesthesia were examined using an animal (rabbit) model. Being started after 1-h anesthesia, the process of accumulation of pathological changes in the cornea unequivocally led clinically significant abrasions following 3-6 h of the narcosis. The corneal damage was associated with alterations in profiles of major proteins of the tear film. Analysis of the tear proteome pointed to depression of lachrymal glands function, and suggested serotransferrin, serum albumin and annexin A1 as potential tear markers of the complication. The tear film alterations included fast drop of total antioxidant activity and activity of superoxide dismutase, and decrease in interleukin-4 and increase in interleukin-6 content indicating development of oxidative and pro-inflammatory responses. These findings suggest antioxidant and anti-inflammatory therapy as prospective approach for prevention/treatment of perioperative corneal abrasions. The observed anesthesia-induced effects should be considered in any study of ocular surface diseases employing anesthetized animals.


PubMed | RAS Engelhardt Institute of Molecular Biology and Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
Type: Journal Article | Journal: Molekuliarnaia biologiia | Year: 2015

Rabies is an infectious disease among humans and animals that remains incurable, despite its longstanding research history. The only way to prevent the disease is prompt treatment, including vaccination as an obligatory component and administration of antirabies immunoglobulin as a supplement. Since the first antirabies vaccination performed in the 19th century, a large number of different rabies vaccines have been developed. Progress in molecular biology and biotechnology enabled the development of effective and safe technologies of vaccine production. Currently, new-generation vaccines are being developed based on recombinant rabies virus strains or on the production of an individual recombinant rabies antigen-glycoprotein (G protein), either as a component of nonpathogenic viruses, or in plants, or in the form of DNA vaccines. In this review, the main modern trends in the development of rabies vaccines have been discussed.

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