Yan J.-L.,Skin Regeneration Medicine |
Yan J.-L.,Liaoning Medical University |
Di G.-H.,Skin Regeneration Medicine |
Ding H.,Skin Regeneration Medicine |
And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Human adipose stem cells have the capability of multipotential differentiation and have a promising prospect in the field of tissue engineering and regenerative medicine. OBJECTIVE: To evaluate the technology and efficacy of human adipose stem cells in autologous fat grafting breast augmentation. METHODS: Fifteen patients with auto-adipose injection breast augmentation were followed-up for 3-6 months. The whole operation was separated into two phases, in the first phase, 15-20 mL adipose tissue was aspirated and digested with collagenase I to separate and amplify the human adipose stem cells. When cultured to passage 2, the plastic adherent stem cells were up to 1×108 and verificated by immunophenotype, differentiation and genetic stability assays before transplantation. In the second phase, 300-450 mL adipose tissue was aspirated and transplanted combined with human adipose stem cells. RESULTS AND CONCLUSION: The passage 2 human adipose stem cells exhibited typical mesenchymal stem cells characteristics and maintained genetic stability. The 3-6 months follow-up demonstrated that there was no apparent adipose absorption, infection, pain and upper limb activity limitation, and the effect was good. The authors suggested that autologous adipose derived stem cells-mediated auto-adipose injection breast augmentation is a practical and efficacious method.