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Kang K.A.,Jeju National University | Zhang R.,Jeju National University | Piao M.J.,Jeju National University | Chae S.,Korea Institute of Oriental Medicine | And 4 more authors.
Toxicology and Industrial Health | Year: 2012

Baicalein (5,6,7-trihydroxyflavone) is a phenolic flavonoid compound derived mainly from the root of Scutellaria baicalensis Georgi, a medicinal plant traditionally used in oriental medicine. In our previous study, baicalein attenuated mitochondrial oxidative stress by scavenging reactive oxygen species (ROS) and by induction of nuclear factor erythroid 2-related factor 2 transcription factor-mediated manganese superoxide dismutase. In the present study, the protective effects of baicalein against oxidative stress-induced damage, especially cellular components including DNA, lipid, and protein, were studied. The results of this study showed that baicalein scavenged intracellular ROS. Baicalein inhibited the H2O2-induced DNA damage that was demonstrated by decreased phospho-H2A.X expression and DNA tail formation. In addition, it prevented the lipid peroxidation shown by the fluorescence intensity of diphenyl-1-pyrenylphosphine and the formation of thiobarbituric acid reactive substances. Moreover, baicalein inhibited protein oxidation demonstrated by protein carbonyl formation. Furthermore, baicalein protected cells via the inhibition of apoptosis induced by H2O2. The findings of this study suggest that baicalein provides protection for cellular components against oxidative damage via scavenging ROS and inhibiting apoptosis. © The Author(s) 2011.

Kim K.C.,Jeju National University | Kang K.A.,Jeju National University | Lim C.M.,Jeju National University | Park J.H.,Skin Cure Life Science Institute | And 2 more authors.
Journal of the Korean Society for Applied Biological Chemistry | Year: 2012

To better understand the molecular mechanisms underlying the anti-aging properties of water extract of edible bird's nest (WEBN), the effect of WEBN on oxidative stressinduced matrix metalloproteinase-1 (MMP-1) was assessed in human HaCaT keratinocyte. Upon exposure to H 2O 2, WEBN inhibited H 2O 2-induced cytotoxicity and scavenged intracellular reactive oxygen species, down-regulated H 2O 2-induced MMP-1 mRNA expression, protein expression and activity, and lowered the activation of extracellular signal-regulated protein kinase (ERK) and c-Jun N-terminal kinases (JNK), which are the upstream of MMP-1 expression. Furthermore, WEBN inhibited the expression of c-Fos and phospho c-Jun, which are components of the transcription factor activator protein-1 (AP-1), AP-1 transcriptional activity, and AP-1 binding to the MMP-1 promoter. These data indicate that the anti-aging properties of WEBN involve the inhibition of MMP-1 expression via down-regulation of the ERK/JNK and AP-1 pathway. © The Korean Society for Applied Biological Chemistry and Springer 2012.

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