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Wang H.,Tianjin University | Mu W.,Tianjin University | Zhai J.,Tianjin University | Xing D.,Tianjin University | And 8 more authors.
Trials | Year: 2013

Background: Diabetic nephropathy (DN) is a major microvascular complication with diabetes. In China, an estimated 34.7 percent of people diagnosed with diabetes have renal complications and a further 50 percent die of renal failure. Hence, identification of alternative treatments for these patients should be given priority. The Shenyan Kangfu tablet (SYKFT) is a new formulation of an existing and widely acclaimed Chinese herbal tea for treating qi-yin deficiency syndrome. Because a considerable portion of DN patients presenting with symptoms of swelling, fatigue and weak limbs would be diagnosed with qi-yin deficiency syndrome according to the traditional Chinese medicine (TCM) diagnostic criteria, we hypothesize that SYKFT may represent a complementary drug for DN patients with the corresponding syndrome. In view of this, we have designed a trial to assess the efficacy and safety of SYKFT for patients with diabetic nephropathy exhibiting signs of qi and yin deficiency.Methods: This is a multicenter, double-blind, randomized controlled trial (RCT). The total target sample size is planned at 80 participants, with a balanced (1:1) treatment allocation. The experimental intervention will be SYKFY plus irbesartan (SI regimen) and the control intervention will be a placebo plus irbesartan (PI regimen). Participants will receive two courses of medication treatment each lasting 8 weeks. The primary outcome will be the composite of the quantitative 24-hour urinary protein level and urinary albumin excretion rate (UAER). Changes in urine albumin-to-creatinine ratio (UACR) and DN staging, and TCM symptom improvement will be the secondary outcome measures. Adverse events (AEs) will be monitored throughout the trial.Discussion: This study will be the first placebo-controlled RCT to assess whether SYKFT plus irbesartan will have beneficial effects on enhancing overall response rate (ORR), changing DN staging, improving clinical symptoms, and reducing the frequency of AEs for DN patients with qi-yin deficiency syndrome. The results of this trial will help to provide evidence-based recommendations for clinicians.Trial registration: Chinese Clinical Trials Register, Identifier: ChiCTR-TRC-12002182. © 2013 Wang et al.; licensee BioMed Central Ltd. Source


Chen Y.,Sixth Peoples Hospital of Shanghai | Xia R.G.,Sixth Peoples Hospital of Shanghai
Genetics and Molecular Research | Year: 2014

We searched for key genes that could accurately predict bone mineral density. The gene expression profile GSE7429 was downloaded from the Gene Expression Omnibus database, which includes 20 samples, 10 with high and 10 with low bone mineral density. The differentially expressed genes (DEGs) were identified with packages in R language. Further, BLASTX was used to obtain COG function classifications of all the DEGs. The GOTM software was used to find DEGs enriched modules. The functions of genes in the modules was also predicted with the software GENECODIS. Three hundred and three genes were identified as DEGs by comparing high and low bone mineral density samples; the selected genes were mapped to 14 modules collected in PPID. Genes VDR, ESR1, and NRIP1, located in the same module, were significantly enriched in intracellular receptor-mediated signaling biological processes. We conclude that the genes VDR, ESR1, NRIP1 in B cells have a close relationship with bone mineral density. The expression patterns of these genes could be used to determine osteoprotegerin function and for early diagnosis and prevention of low bone mineral density. © FUNPEC-RP. Source


Li S.-s.,Shanghai University of Traditional Chinese Medicine | Wu Y.-c.,Sixth Peoples Hospital of Shanghai | Zhang J.-f.,Sixth Peoples Hospital of Shanghai | Zhou J.-h.,Shanghai University of Traditional Chinese Medicine | And 2 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Studies have shown that matrix metalloproteinases 3 plays an important role in articular cartilage degeneration and destruction. OBJECTIVE: To observe the changes of metalloproteinases 3 expression level in synovium of knee osteoarthritis rats after treated with penetration needling of long needle and sodium hyaluronate. METHODS: SD rats were injected with 1. 6% papain solution to knee joint cavity and were driven to exercise in order to construct knee osteoarthritis model. Rats were randomly assigned into 3 groups in the 2nd week after model construction: penetration needling group, drug group and model group. Penetration needling group were treated by penetration needling with filiform needle, with a diameter of 0. 30 millimeters and a length of 125 milimeters. Drug group were injected with sodium hyaluronate to knee joint cavity. Model group received no treatment. And non-model control group were established. RESULTS AND CONCLUSION: Content of synovium matrix metalloproteinase 3 in model group was significantly increased than that in the control group (P < 0. 05) in the 4th week after treatment. The content of matrix metalloproteinase 3 in penetration needling group and drug group was significantly reduced than that in the model group (P < 0. 05). Penetration needling group was similar to drug group (P > 0. 05). These findings demonstrate that long needle penetration therapy can effectively correct the abnormal expression of matrix metalloproteinase 3 in synovium of knee osteoarthritis model rats. It may be one of the mechanisms of the treatment of knee osteoarthritis by long needle penetration needling. Source


Wang Z.,Sixth Peoples Hospital of Shanghai | Yang C.,Sixth Peoples Hospital of Shanghai | Yang Y.,Sixth Peoples Hospital of Shanghai | Shen Z.,Sixth Peoples Hospital of Shanghai | And 2 more authors.
Chinese-German Journal of Clinical Oncology | Year: 2011

Objective: The purpose of this study was to assess the clinical application value of bone turnover markers in non-small-cell lung cancer (NSCLC) patients with bone metastases. Including diagnosing bone metastases, detecting bone metastatic spread. Methods: Alkaline phosphatase (AKP), β-C-terminal telopeptide of type I collagen (β-CTx), osteocalcin (OST) and bone alkaline phosphatase (BALP) were measured in 76 patients with bone metastases from NSCLC and 44 normal people. Results: The level of AKP, β-CTx and BALP in patients with bone metastasis was significantly higher than in the normal people. Significant correlation was observed among bone turnover markers. The levels of BALP and OST were significantly correlated with the extent of bone metastasis. The patients with high-level CTx and low-level BALP had higher risk of pathologic fracture. Conclusion: In NSCLC patients with bone metastases, bone turnover markers can help to make diagnosis and evaluate the severity. It will have a wide range of use in clinical practice. © Huazhong University of Science and Technology and Springer-Verlag 2011. Source


Zhang K.-L.,Sixth Peoples Hospital of Shanghai | Wang Y.,Sixth Peoples Hospital of Shanghai | Guo X.-R.,Donghua University | Chen J.-F.,Donghua University | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: The electrospinning technique has been used to prepare biological scaffolds to simulate nano-fiber structure of extracellular matrix; therefore, widespread attention has been paid to the electrospinning technique in the field of regenerative medicine and tissue engineering. OBJECTIVE: To review the articles about increasing electrospun nanofiber scaffold porosity, enlarging pore diameter, promoting cell infiltration with related technologies, in order to discover the most practical and economical technology. METHODS: The first author retrieved CNKI database, Wanfang database and PubMed with the keywords of “cell infiltration, 3D scaffold, electrospinning” in Chinese and English, respectively. Literature retrieval period was from January 2004 to October 2014. RESULTS AND CONCLUSION: Electrospinning technology is the most effective method for preparation of nanofiber scaffolds. Electrospinning scaffolds as tissue engineering scaffolds have become an issue of concern in the basic research year by year. However, the internal nano-scale pore of nanofiber scaffolds limits the cells to grow on the surface, so recent research has been focused on highly porous three-dimensional structure which can promote the permeable growth of cells instead of two-dimensional scaffolds. Several techniques have been used, which go from the adjustment of materials and speed of electrospinning to the applications of various kinds of complicated machines. However, the existing researches are still not mature and stable, the majority of which are applied only in vitro as cell implantation or subcutaneous implantation in small animals. The above-mentioned methods still need long-term comparative studies to confirm the feasibility in the tissue-engineered repair of organs. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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