Sixth Peoples Hospital of Shanghai
Sixth Peoples Hospital of Shanghai
Li H.,Shanghai JiaoTong University |
Liu P.,Shanghai JiaoTong University |
Xu S.,Shanghai JiaoTong University |
Li Y.,Fudan University |
And 11 more authors.
Journal of Clinical Investigation | Year: 2017
FOXA hallmark of aged mesenchymal stem/progenitor cells (MSCs) in bone marrow is the pivot of differentiation potency from osteoblast to adipocyte coupled with a decrease in self-renewal capacity. However, how these cellular events are orchestrated in the aging progress is not fully understood. In this study, we have used molecular and genetic approaches to investigate the role of forkhead box P1 (FOXP1) in transcriptional control of MSC senescence. In bone marrow MSCs, FOXP1 expression levels declined with age in an inverse manner with those of the senescence marker p16INK4A. Conditional depletion of Foxp1 in bone marrow MSCs led to premature aging characteristics, including increased bone marrow adiposity, decreased bone mass, and impaired MSC selfrenewal capacity in mice. At the molecular level, FOXP1 regulated cell-fate choice of MSCs through interactions with the CEBP?/? complex and recombination signal binding protein for immunoglobulin ? J region (RBPj?), key modulators of adipogenesis and osteogenesis, respectively. Loss of p16INK4A in Foxp1-deficient MSCs partially rescued the defects in replication capacity and bone mass accrual. Promoter occupancy analyses revealed that FOXP1 directly represses transcription of p16INK4A. These results indicate that FOXP1 attenuates MSC senescence by orchestrating their cell-fate switch while maintaining their replicative capacity in a dose-and age-dependent manner.
Wang H.,Tianjin University |
Mu W.,Tianjin University |
Zhai J.,Tianjin University |
Xing D.,Tianjin University |
And 8 more authors.
Trials | Year: 2013
Background: Diabetic nephropathy (DN) is a major microvascular complication with diabetes. In China, an estimated 34.7 percent of people diagnosed with diabetes have renal complications and a further 50 percent die of renal failure. Hence, identification of alternative treatments for these patients should be given priority. The Shenyan Kangfu tablet (SYKFT) is a new formulation of an existing and widely acclaimed Chinese herbal tea for treating qi-yin deficiency syndrome. Because a considerable portion of DN patients presenting with symptoms of swelling, fatigue and weak limbs would be diagnosed with qi-yin deficiency syndrome according to the traditional Chinese medicine (TCM) diagnostic criteria, we hypothesize that SYKFT may represent a complementary drug for DN patients with the corresponding syndrome. In view of this, we have designed a trial to assess the efficacy and safety of SYKFT for patients with diabetic nephropathy exhibiting signs of qi and yin deficiency.Methods: This is a multicenter, double-blind, randomized controlled trial (RCT). The total target sample size is planned at 80 participants, with a balanced (1:1) treatment allocation. The experimental intervention will be SYKFY plus irbesartan (SI regimen) and the control intervention will be a placebo plus irbesartan (PI regimen). Participants will receive two courses of medication treatment each lasting 8 weeks. The primary outcome will be the composite of the quantitative 24-hour urinary protein level and urinary albumin excretion rate (UAER). Changes in urine albumin-to-creatinine ratio (UACR) and DN staging, and TCM symptom improvement will be the secondary outcome measures. Adverse events (AEs) will be monitored throughout the trial.Discussion: This study will be the first placebo-controlled RCT to assess whether SYKFT plus irbesartan will have beneficial effects on enhancing overall response rate (ORR), changing DN staging, improving clinical symptoms, and reducing the frequency of AEs for DN patients with qi-yin deficiency syndrome. The results of this trial will help to provide evidence-based recommendations for clinicians.Trial registration: Chinese Clinical Trials Register, Identifier: ChiCTR-TRC-12002182. © 2013 Wang et al.; licensee BioMed Central Ltd.
Xia H.-J.,Sixth Peoples Hospital of Shanghai |
Xia H.-J.,Shanghai JiaoTong University |
Wang Y.-P.,Sixth Peoples Hospital of Shanghai |
Zhu W.,Sixth Peoples Hospital of Shanghai |
And 2 more authors.
Chinese Pharmacological Bulletin | Year: 2014
Aim: To observe the protective effect of resveratrol on doxorubicine (DOX)-induced injury in H9c2 cardiac cells and the underlying mechanism. Methods: H9c2 cells were treated with DOX to establish a model of cardiomyocyte injury. Resveratrol was applied before exposure to DOX to activate SIRT1, and SIRT1 siRNA was used to inhibit SIRT1. MTT was used to detect the viability of H9c2 cells. Flow cytometry was used to determine the apoptosis of H9c2 cells. The expression of SIRT1 was detected by Western blot. H9c2 cells transfected with GFP were observed under a fluorescence microscope, and the transfection efficiency was calculated in a randomly-selected view. Results: DOX decreased cell viability and induced apoptosis in time-and dose-dependent pattern. In contrast ,SIRT1 protein was significantly upregulated by RSV also in a time- and dose-dependent way. Pre-treatment with RSV effectively reduced injury of H9c2 cells induced by DOX. Pre-treatment with SIRT1 siRNA aggravated the injury of H9c2 cells induced by DOX, which could not be alleviated by RSV treatment. Conclusion: RSV can significantly protect H9c2 cells against DOX induced injury possibly by upregulating the expression of SIRT1 protein.
PubMed | Shanghai Qisheng Institute for Biological Material Research Shanghai, Shanghai JiaoTong University and Sixth Peoples Hospital of Shanghai
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2015
Calcium alginate has been proved to favor the skin ulcer healing and collagen synthesis was a critical factor for the wound closure. The present study was to elucidate the mechanism of calcium alginate on the diabetes skin ulceration. Calcium alginate dressing was applied daily on the full-thickness exercising wound created on the back of diabetic rat model as Alg-group (n=6), and the vaseline dressing was used as control (n=6). Rats were respectively sacrificed and the wound tissues were removed and used for the evaluation of various biochemical analysis contained collagen (type I and III) by Western blotting and hydroxyproline level changes by ELISA assay at 3 d, 7 d and 14 d after wounding. The expression of skin collagen I in Alg-group was enhanced from day 3 (0.66 0.25 vs. 0.42 0.09, P<0.05) to day 14 (1.09 0.14 vs. 0.78 0.16, P<0.05). However, no significant difference of collagen III expression was found between two groups during wound healing (P>0.05). And the ratio of collagen I/III in Alg-group was greater than that of Vas-group at day 7 (1.07 0.31 vs. 0.77 0.11, P<0.05) and 14 (1.18 0.30 vs. 0.83 0.14, P<0.05). The hydroxyproline level in skin homogenate of Alg-group was higher than that of Vas-group from day 3 (30.29 0.92 ng/ml vs. 27.52 0.83 ng/ml, P<0.05) to day 14 (89.58 4.97 ng/ml vs. 79.30 4.42 ng/ml, P<0.05). Calcium alginate accelerates the process of wound healing through improving type I collagen synthesis and increasing ratio of collagen I/III in diabetic rats.
Zhang K.-L.,Sixth Peoples Hospital Of Shanghai |
Wang Y.,Sixth Peoples Hospital Of Shanghai |
Guo X.-R.,Donghua University |
Chen J.-F.,Donghua University |
And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: The electrospinning technique has been used to prepare biological scaffolds to simulate nano-fiber structure of extracellular matrix; therefore, widespread attention has been paid to the electrospinning technique in the field of regenerative medicine and tissue engineering. OBJECTIVE: To review the articles about increasing electrospun nanofiber scaffold porosity, enlarging pore diameter, promoting cell infiltration with related technologies, in order to discover the most practical and economical technology. METHODS: The first author retrieved CNKI database, Wanfang database and PubMed with the keywords of “cell infiltration, 3D scaffold, electrospinning” in Chinese and English, respectively. Literature retrieval period was from January 2004 to October 2014. RESULTS AND CONCLUSION: Electrospinning technology is the most effective method for preparation of nanofiber scaffolds. Electrospinning scaffolds as tissue engineering scaffolds have become an issue of concern in the basic research year by year. However, the internal nano-scale pore of nanofiber scaffolds limits the cells to grow on the surface, so recent research has been focused on highly porous three-dimensional structure which can promote the permeable growth of cells instead of two-dimensional scaffolds. Several techniques have been used, which go from the adjustment of materials and speed of electrospinning to the applications of various kinds of complicated machines. However, the existing researches are still not mature and stable, the majority of which are applied only in vitro as cell implantation or subcutaneous implantation in small animals. The above-mentioned methods still need long-term comparative studies to confirm the feasibility in the tissue-engineered repair of organs. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Wang Z.,Sixth Peoples Hospital of Shanghai |
Yang C.,Sixth Peoples Hospital of Shanghai |
Yang Y.,Sixth Peoples Hospital of Shanghai |
Shen Z.,Sixth Peoples Hospital of Shanghai |
And 2 more authors.
Chinese-German Journal of Clinical Oncology | Year: 2011
Objective: The purpose of this study was to assess the clinical application value of bone turnover markers in non-small-cell lung cancer (NSCLC) patients with bone metastases. Including diagnosing bone metastases, detecting bone metastatic spread. Methods: Alkaline phosphatase (AKP), β-C-terminal telopeptide of type I collagen (β-CTx), osteocalcin (OST) and bone alkaline phosphatase (BALP) were measured in 76 patients with bone metastases from NSCLC and 44 normal people. Results: The level of AKP, β-CTx and BALP in patients with bone metastasis was significantly higher than in the normal people. Significant correlation was observed among bone turnover markers. The levels of BALP and OST were significantly correlated with the extent of bone metastasis. The patients with high-level CTx and low-level BALP had higher risk of pathologic fracture. Conclusion: In NSCLC patients with bone metastases, bone turnover markers can help to make diagnosis and evaluate the severity. It will have a wide range of use in clinical practice. © Huazhong University of Science and Technology and Springer-Verlag 2011.
Wang T.,Sixth Peoples Hospital of Shanghai |
Chen J.-F.,Donghua University |
Zhao J.,Sixth Peoples Hospital of Shanghai |
Wu T.,Donghua University |
And 6 more authors.
Chinese Journal of Biomedical Engineering | Year: 2014
To evaluate the biodegradation and biocompatibility of poly (L-lactic acid-co-e-caprolactone) (P (LLA-CL))/collagen graft, P (LLA-CL) collagen graft scaffolds were made through electrospinning. The prepared scaffolds were set as experimental group (n = 40) and the commercial artificial vessel made of ePTFE was set as control group (n = 40). The scaffold samples were implanted in the left dorsal muscle of rabbits, while ePTFE graft materials were implanted in the opposite side. On day 10, 30, 90 and 180 after the implantation, the samples were harvested. The histological staining was applied to observe the degradation of the material and tissue reaction. The number of neutrophils, macrophages and lymphocytes were counted at the high magnification (400 x) using the optical microscope. Under the microscope, slight tissue reaction was revealed in the area of P (LLA-CL)/collagen material. On day 90, the material broken was observed. Some connective fibrous tissue was revealed in the material, a capsule-like membrane was established over the area. During the process of degradation, most of the materials were absorbed within 180 days. The structure and distribution of the tissue cells was highly similar to that of the surrounding area, indicating that the remodeling of the embedded tissue was almost finished. The ePTFE materials were revealed still maintaining the structure encompassed in a thin layer of fibrous connective tissue. The result of cell count showed that the type and trend of cell reaction had no difference after the different stages of post-operation. However, the count of neutrophile granulocyte in experimental group was more than that in the control group on day 10 (224. 4 ± 37. 2 vs 192. 2 ± 18. 1/0. 25 mm2, P <0. 05), while no difference of various types of cells was observed between the two groups post operation. The degeneration of P (LLA-CL)/collagen material was 90 - 180 days, which is a satisfying biological performance in the degradation and biocompatibility.
Chen Y.,Sixth Peoples Hospital of Shanghai |
Xia R.G.,Sixth Peoples Hospital of Shanghai
Genetics and Molecular Research | Year: 2014
We searched for key genes that could accurately predict bone mineral density. The gene expression profile GSE7429 was downloaded from the Gene Expression Omnibus database, which includes 20 samples, 10 with high and 10 with low bone mineral density. The differentially expressed genes (DEGs) were identified with packages in R language. Further, BLASTX was used to obtain COG function classifications of all the DEGs. The GOTM software was used to find DEGs enriched modules. The functions of genes in the modules was also predicted with the software GENECODIS. Three hundred and three genes were identified as DEGs by comparing high and low bone mineral density samples; the selected genes were mapped to 14 modules collected in PPID. Genes VDR, ESR1, and NRIP1, located in the same module, were significantly enriched in intracellular receptor-mediated signaling biological processes. We conclude that the genes VDR, ESR1, NRIP1 in B cells have a close relationship with bone mineral density. The expression patterns of these genes could be used to determine osteoprotegerin function and for early diagnosis and prevention of low bone mineral density. © FUNPEC-RP.
Li S.-s.,Shanghai University of Traditional Chinese Medicine |
Li S.-s.,Sixth Peoples Hospital of Shanghai |
Wu Y.-c.,Sixth Peoples Hospital of Shanghai |
Zhang J.-f.,Sixth Peoples Hospital of Shanghai |
And 3 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011
BACKGROUND: Studies have shown that matrix metalloproteinases 3 plays an important role in articular cartilage degeneration and destruction. OBJECTIVE: To observe the changes of metalloproteinases 3 expression level in synovium of knee osteoarthritis rats after treated with penetration needling of long needle and sodium hyaluronate. METHODS: SD rats were injected with 1. 6% papain solution to knee joint cavity and were driven to exercise in order to construct knee osteoarthritis model. Rats were randomly assigned into 3 groups in the 2nd week after model construction: penetration needling group, drug group and model group. Penetration needling group were treated by penetration needling with filiform needle, with a diameter of 0. 30 millimeters and a length of 125 milimeters. Drug group were injected with sodium hyaluronate to knee joint cavity. Model group received no treatment. And non-model control group were established. RESULTS AND CONCLUSION: Content of synovium matrix metalloproteinase 3 in model group was significantly increased than that in the control group (P < 0. 05) in the 4th week after treatment. The content of matrix metalloproteinase 3 in penetration needling group and drug group was significantly reduced than that in the model group (P < 0. 05). Penetration needling group was similar to drug group (P > 0. 05). These findings demonstrate that long needle penetration therapy can effectively correct the abnormal expression of matrix metalloproteinase 3 in synovium of knee osteoarthritis model rats. It may be one of the mechanisms of the treatment of knee osteoarthritis by long needle penetration needling.
Wu Y.-J.,Sixth Peoples Hospital of Shanghai |
Sun J.,Sixth Peoples Hospital of Shanghai |
Zhang J.-H.,Sixth Peoples Hospital of Shanghai |
Feng L.-L.,Sixth Peoples Hospital of Shanghai
Chinese Journal of Contemporary Pediatrics | Year: 2014
Objective: To study the clinical efficacy of adjuvant therapy with glucocorticoids in children with lobar pneumonia caused by Mycoplasma pneumoniae. Methods: One hundred and eight children with lobar pneumonia caused by Mycoplasma pneumoniae were randomly divided into routine treatment and hormone treatment groups. Both groups were treated with azithromycin and other symptomatic therapies. In addition to the basic treatment, the hormone treatment group was given dexamethasone 0.25-0.3 mg/(kg·d) by intravenous drip until the body temperature was normal. Then given oral prednisone tablets 0.5-1 mg/(kg·d) (gradually reduced) for a total treatment course of 7-10 days. Before and after treatment pulmonary functions were examined, and serum C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), interleukin-2 (IL-2) and interleukin-6 (IL-6) were measured. Results: The duration of fever, cough relief time and pulmonary shadow absorption time on chest X-ray were significantly shorter in the hormone treatment group than in the routine treatment group (P<0.05). After treatment, the two groups showed improvements in serum CRP, ESR, IL-2, and IL-6 (P<0.05), but the hormone treatment group showed significantly more improvement (P<0.05). Varying degrees of mixed ventilation dysfunction were seen in the two groups before treatment, and hormone therapy significantly improved pulmonary function, especially promoting the recovery of small airway function. Conclusions: Adjuvant therapy with glucocorticoids can effectively alleviate clinical symptoms, promote the absorption of pulmonary inflammation, and improve pulmonary function in children with lobar pneumonia caused by Mycoplasma pneumoniae.