Song T.,First Hospital of Shijiazhuang |
Wang L.,Sixth Hospital of Shijiazhuang |
Gu K.,First Hospital of Shijiazhuang |
Yang Y.,Hebei Medical University |
And 9 more authors.
Neurochemistry International | Year: 2015
Thalidomide was introduced to the market in 1957 as a sedative and antiemetic agent, and returned to the market for the treatment of myelodysplastic syndrome and multiple myeloma. There are reports and studies of thalidomide as an analgesic or analgesic adjuvant in clinic. However, the underlying mechanism is quite elusive. Many studies suggest that the analgesic effect of thalidomide may be due to its immunomodulatory and anti-inflammatory properties as it suppresses the production of tumor necrosis factor α (TNF-α) selectively. However, it is not clear whether any other mechanisms are implicated in the pain relief. In this study, we demonstrated that the peripheral vanilloid receptor 1 (TRPV1) channel was also involved in the analgesic effect of thalidomide in different cell and animal models. During the activation by its agonist capsaicin, the cation inward influx through TRPV1 channels and the whole-cell current significantly decreased after TRPV1-overexpressed HEK293 cells or dorsal root ganglion (DRG) neurons were pre-treated with thalidomide for 20 minutes. And such attenuation in the TRPV1 activity was in a dose-dependent manner of thalidomide. In an acetic acid writhing test, pre-treatment of thalidomide decreased the writhing number in the wild type mice, while it did not happen in TRPV1 knockout mice, suggesting that the TRPV1 channel was involved in the pain relief by thalidomide. Taken together, the study showed that TRPV1 channels were involved in the analgesic effects of thalidomide. Such alteration in the action of TRPV1 channels by thalidomide may help understand how thalidomide takes analgesic effect in the body in addition to its selective inhibition of TNF-α production. © 2015 Elsevier Ltd. All rights reserved.
Li H.N.,Sixth Hospital of Shijiazhuang
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2013
To investigate the role of heme oxygenase and carbon monoxide (HO/CO) in the development of spontaneous pain and hyperalgesia of rats induced by formalin injection. Zinc protoporphyrin Znpp (the inhibitor of HO) was intrathecally injected to the rats with formalin inflammatory pain. Hemin (the agonist of HO) was intrathecally injected to the normal rats. The weighted pain scores were used to evaluate the degree of pain response. Thermal withdrawal latency and mechanical withdrawal threshold were observed to assess the degree of thermal hyperalgesia and mechanical allodynia. After the intrathecal injection of Znpp, the weighted pain score obviously reduced in a dose-dependent manner compared with the rats with formalin inflammatory pain. Intrathecal injection of Znpp had no obvious effect on thermal withdrawal latency and mechanical withdrawal threshold in injected feet compared with formalin group. But there was a prolongation in a dose-dependent manner in non injected feet. Intrathecal injection of Hemin to normal rats could shorten the thermal withdrawal latency and reduce the mechanical withdrawal threshold on both sides of hindpaws. Intrathecal injection of the HO inhibitor produced prominent inhibition to pain related behavior and thermal and mechanical hyperalgesia induced by formalin injection. Intrathecal injection of HO inductor could induce thermal and mechanical hyperalgesia in normal rats. The results indicated that HO/CO took part in the processes of spinal cord nociceptive information transmission and the development of thermal and mechanical hyperalgesia.
Huang Y.,Sixth Hospital of Shijiazhuang |
Guo N.,Sixth Hospital of Shijiazhuang
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Recent studies have shown that osteoclast differentiation factor is closely related to osteoclast differentiation, formation and function in bone remodeling during orthodontic tooth movement. OBJECTIVE: To observe the effects of three kinds of orthodontic appliances on the expression of osteoclast differentiation factor at the pressure side of rat periodontal tissue during remodeling process and to explore the biocompatibility of the orthodontic appliances with the host tissues during orthodontic treatment. METHODS: Eighty healthy Wistar rats were selected to establish animal models of orthodontic tooth movement, and then randomly divided into four groups: control group, MBT group, Begg group, Damon III appliance group. Four animal from each group were sacrificed at 3, 7, 14 days after wearing orthodontic appliances. The tartrate-resistant acid phosphatase staining was used to count the osteoclasts at the pressure side of alveolar bone tissue; real-time quantitative PCR detection to detect mRNA expression of osteoclast differentiation factor at the pressure side of periodontal tissue and time distribution characteristics. RESULTS AND CONCLUSION: Compared with the control group, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were increased with orthodontic time, reached the peaked at 7 days and then gradually decreased. The number of positive osteoclasts and mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were significantly higher in the Damon III group than the other three groups at 7 days after orthodontic treatment (P < 0.05). These findings indicate that, during the bone remodeling, the number of positive osteoclasts changed in accordance with the mRNA expression of osteoclast differentiation factor, and at 7 days, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor were highest in the Damon III group. © 2015, Chinese Journal of Tissue Engineering Research. All rights reserved.
Huang Y.,Sixth Hospital of Shijiazhuang |
Guo N.,Sixth Hospital of Shijiazhuang
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: There is no design that can completely rule out the intermittent impact damage to implants, therefore, a new ball attachment-retained implant system is constantly updated and developed. OBJECTIVE: To evaluate the clinical effectiveness of an implant-supported mandibular overdenture retained with a ball attachment. METHODS: We searched the Cochrane Library, PubMed, Medline, EM-base, WanFang Data, CNKI, VIP and other databases by computer to collect randomized controlled trials addressing the implant-supported mandibular overdenture retained with a ball attachment and other control methods for dentures. The time limit was from database creation to February 2014. Two researchers independently completed literature screening according to inclusion and exclusion criteria, data extraction and quality assessment. RevMan 5.1 software was used for meta-analysis. RESULTS AND CONCLUSION: There were 10 studies included in result analysis, including 7 from China and 3 from other countries. Analysis results showed that statistical heterogeneity was remarkable in included studies, and there was no significant difference in patient’s satisfaction, clinical and objective indicators, and complications, suggesting that this approach continues to be explored in clinic. The implant-supported ball attachment-retained mandibular overdenture is relatively expensive, which is identical with the current research progress that is in the exploration stage worldwide. Due to the limited quantity and quality of included studies, the conclusions of this systematic review only provide references for clinical practice and research. The implant-supported ball attachment-retained mandibular overdenture still needs further exploration and improvement. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.