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Khan P.S.,Sir Syed Institute For Technical Studies | Skandhan K.P.,Sree Narayana institute of medical science | Ajesh K.,Sir Syed Institute For Technical Studies | Siraj M.V.P.,Sir Syed Institute For Technical Studies
Biological Trace Element Research | Year: 2011

The study included evaluation of semen from normal, healthy adults and estimation of gold in seminal plasma from two regions Nilambur valley (Malappuram District, Kerala Province, India; n=11), where gold deposit is present and Kollam town (Kerala Province, India; n=13) where gold is not present in soil. All samples showed normal spermiogram. Gold was estimated in seminal plasma by employing inductively coupled plasma emission spectrophotometry. It varied from 0.23-1.15 ppm with mean 0.68 ppm in study area while in control area it was 0.13-0.71 ppm with a mean of 0.41 ppm. Statistical studies (independent 't' test) showed significantly high level (p<0.05) of gold in seminal plasma from study area. ANOVA test proved that gold in seminal plasma is a significant (p<0.05) factor for total spermatozoa count and its percentage of motility. © Springer Science+Business Media, LLC 2010. Source


Valsa J.,Government Medical College | Skandhan K.P.,Sree Narayana institute of medical science | Sumangala B.,Sree Narayana institute of medical science | Sahab Khan P.,Sir Syed Institute For Technical Studies | Amith S.,Southeast Alabama Medical Center
Journal of Clinical Laboratory Analysis | Year: 2012

Background: In laboratories, the semen or seminal plasma samples are stored before estimation of different elements without knowing the adverse effect of storing. The present study shows the levels of Ca++ and Mg++ in the seminal plasma before and after the storage. Methods: Atomic absorption spectrophotometry (AAS) was used for estimating calcium and magnesium levels in the seminal plasma. Samples were stored for one year in different containers such as polyethylene, plastic, ordinary glass, and borosil glass. Calcium and magnesium levels were estimated after this period by AAS. The total area in contact with containers was considered in the final calculation. Results: After 1-year storage, the levels of calcium and magnesium had changed. The most suitable container for storage of calcium was ordinary glass (P > 0.05) whereas ordinary glass (P > 0.05), polyethylene (P > 0.05), and borosil glass (P > 0.05) are the most suitable for magnesium. Conclusion: In any elemental study, the most suitable containers are recommended for storage of the semen/seminal plasma samples. © 2012 Wiley Periodicals, Inc. Source


Valsa J.,Government Medical College | Skandhan K.P.,Government Medical College | Skandhan K.P.,Sree Narayana institute of medical science | Gusani P.,Government Medical College | And 3 more authors.
Revista Internacional de Andrologia | Year: 2013

Objective: Different aspects of semen study improved the knowledge of male reproductive system. Aim of the present study is to find out the effect of daily ejaculation for 10 days on semen quality and the level of calcium and magnesium in semen. Materials and methods: Twenty-one young healthy sexually active men participated in this study. Their consent was collected prior to it. Effect of daily ejaculation for 10 days on semen parameters and calcium and magnesium it were studied. After an abstinence of 3-5 days 11th sample was collected. On a daily basis samples were evaluated and calcium and magnesium were estimated in spermatozoa as well as seminal plasma. Results: The study showed quality of semen improved by daily ejaculation. Statistically no differences in the level of calcium and magnesium in seminal plasma and spermatozoa were observed. Conclusion: The study proved daily ejaculation was useful in improving its quality. © 2012 Asociación Española de Andrología, Medicina Sexual y Reproductiva. Source

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