Sir Hn Medical Research Society
Sir Hn Medical Research Society
PubMed | Sir Hn Hospital And Research Center and Sir Hn Medical Research Society
Type: Journal Article | Journal: Indian dermatology online journal | Year: 2013
Vitiligo is an acquired skin disease that involves the interplay of complex genetic, immunological, neural and self-destructive mechanisms in its pathogenesis. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration.The aim of our investigation was to evaluate the role of oxidative stress by studying the role of catalase (CAT) in the destruction of melanocytes in patients with vitiligo and compare the same in healthy normal controls.We determined the serum catalase enzyme by ELISA method. The catalase activity was studied in two groups, Group I-localized vitiligo: (i) active stage, (ii) static or inactive stage and Group II-generalized vitiligo: (i) active stage, (ii) static or inactive stage patients, and the levels were compared with healthy controls.Group I active stage patients showed significant difference in the catalase levels with a P < 0.044 when compared with healthy controls, whereas Group II static stage patients did not show any significant difference (P < 0.095) although the catalase activity was increased.Our study could not explain the cause of melanocyte damage in patients in the active stage of the disease. The increase in the oxidative stress as detected by catalase activity was more significant in Group I active disease than Group II active disease patients although the levels were higher than the healthy normals. This is the first study conducted on active and static stage of vitilgo in India. It is possible that the number of compounds of hydrogen peroxide produced is not balanced by the production of catalase in the body.
Deo S.S.,Sir Hn Medical Research Society |
Chogle A.,Sirhn Hospital And Research Center |
Mistry K.J.,Sir Hn Medical Research Society |
Shetty R.R.,Sir Hn Medical Research Society |
Londhe S.B.,Sirhn Hospital And Research Center
Research Journal of Immunology | Year: 2010
The aim of this study were to establish (1) an easy diagnostic criterion for recent-onset arthritis using Rheumatoid Factor (RF) isotypes and anti-cyclic citrullinated peptide (anti-CCP) antibodies. (2) Antigen recognition by anti-citrullinated peptide antibodies (ACPA) in undifferentiated arthritis (UA) and rheumatoid arthritis (RA) patients. This study was conducted on 80 patients of (1) UA (n = 40, < 1 year) and (2) established RA (n = 40, ≥ 1 year), using blood and Synovial fluid. Controls were (1) SLE patients (n = 15) and (2) Blood bank donors (n = 36). The antigen recognition was judged by serum reactivity to protein extracts. (1) Human Placenta (HP) (2) Synovial Tissue (ST) and (3) Synovial Fluid (SF) using Western blot analysis. Specificity and PPV was 78 and 33%. SF and serum levels of IgA-RF, IgM-RF and anti-CCP antibodies by ELISA showed significant correlation (p<0.01). Association becomes stronger with CCP IgM or CCP IgA-RF (p = 0.0001) 30/55 anti-CCP showed antigen recognition to ~ 55 and ~ 200 kDa. ACPA recognition of citrullinated vimentin seen only in RA patients. © 2010 Asian Network for Scientific Information.
PubMed | Sir Hn Medical Research Society
Type: Journal Article | Journal: Journal of laboratory physicians | Year: 2011
Study was undertaken to analyze the frequency of anti-viral citrullinated peptide (anti-VCP) antibodies in sera from patients with early rheumatoid arthritis (ERA).Viral citrullinated peptide (VCP) and Epstein-Barr nuclear antigen (EBNA-1) peptide were commercially prepared and antibodies to these were determined in 25 patients of ERA, 40 disease control patients constituting 25 rheumatoid arthritis (RA), 7 systemic lupus erythematosus (SLE), 2 scleroderma, 1 spondyloarthritis (SpA), 1 juvenile rheumatoid arthritis (JRA), 1 osteoarthritis (OA), 1 psoriatic arthritis (PsA), 1 undifferentiated arthritis (UA), and 1 gout and 25 healthy controls (HCs) were taken for comparison. In-house ELISA was established for both the antibodies while cyclic citrullinated peptide (CCP) antibody was detected by commercial ELISA kit.Significant increase in VCP antibody by ERA and disease controls than healthy normal was observed. VCP IgM antibody was significantly increased in RA patients than HC. The presence of VCP antibody signifies a good marker for ERA. We observed significant difference in the VCP IgG and IgM antibody when compared to EBNA-1. In-house ELISA established for EBNA-1 and VCP antibodies showed low sensitivity but 96% specificity.We observed that sera from early RA patients reacted to the deiminated protein encoded by Epstain Barr Virus (EBV). Thus a possible role of virus in inducing an anti-citrullinated peptide antibody (ACPA) response reveals viral etiology in this disease.