Siping Center Hospital
Siping Center Hospital
Yang X.,Liaoning Medical University |
Yang X.,Northern Hospital |
Liu Y.,Siping Center Hospital |
Zong Z.,Liaoning Medical University |
Tian D.,Liaoning Medical University
Biomedicine and Pharmacotherapy | Year: 2010
Fasudil, an inhibitor of Rho kinase, is known to suppress tumorigenicity and cancer metastasis. However, the underlying molecular mechanisms of how fasudil suppresses cell metastasis have not been fully elucidated. The purpose of this study was to determine the effects of fasudil on migration and cancer growth and to evaluate Rho kinase activity in the 95-D lung carcinoma cell line. The cytotoxic effect of drugs on 95-D cells was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Treatment with fasudil inhibited the growth of 95-D cells in a dose-dependent manner, and the IC50 of fasudil was approximately 0.79 mg/ml (95% confidence limits (CL): 0.58-1.11 mg/ml). The total amounts of active MMP2 and MMP9 per microgram of protein when treated with 0.75 mg/ml fasudil and using the gelatinase assay were decreased compared with the control group by about 22.7% (P < 0.05) and 65.9% (P < 0.01) respectively. Although ABCC1, ABCC3, ABCA3, and ABCC5 were over-expressed at the mRNA level, ABCE1 was the only transporter responsible for resistance in this study. We also found that myosin phosphatase targeting subunit 1 (MYPT1) phosphorylation at Thr-696, which provided direct evidence of Rho kinase activity, was reduced by 29.4% in response to fasudil compared with the control group (P < 0.05). Taken together, our findings show that fasudil prevents cancer metastasis by inhibiting the Rho/Rho kinase pathway and that the ABCE1 gene was involved in the migration of 95-D cells. Crown Copyright © 2009.
Liu J.,Jilin University |
Wang X.-G.,Jilin University |
Zhang S.,Tianjin Nan Kai Hospital |
Wang L.,Jilin University |
And 5 more authors.
Journal of Neurological Sciences | Year: 2014
The aim of this study is to perform a C7 transfer from the uninjured side for the patients with whole brachial plexus nerve root avulsion, and observe the recovery outcomes of affected limb. The 36 patients with whole brachial plexus nerve avulsion who received a C7 transfer from uninjured side for repair by stages were selected. Carrying out follow-up visits, evaluating limb functions on uninjured side, and making comprehensive assessment were in accordance with U.K. MRC scale for sensation and U.K. amended MRC scale for muscular strength. The results indicated that there were 2 patients with effective recovery of supraspinatus muscular strength, 2 of deltoid muscular strength, 6 of biceps brachii muscular strength, 7 of triceps brachii muscular strength, 4 of extensor carpi muscular strength, and 5 of flexor carpi and flexor digitorum muscular strength. Six months after operation, there were 7 patients whose latissimus dorsi muscular strength reached M5-, 7 triceps brachii muscular strength reached M5-, and 10 extensor digitorum muscular strength reached M5-. Three days after operation, there were 3 patients whose limb on uninjured side reached Sensation Grade S4, 12 reached Grade S3+, 11 reached Grade S3, and 10 reached Grade S2. Majority of patients recovered to sensory function S4 and their two-point discrimination approximately recovered to normal 1 year after operation. In conclusion, repair with C7 transfer from the uninjured side is currently an effective and feasible method to repair the functions of the affected limb in brachial plexus nerve root avulsion. © 2014 Ege University Press. All rights reserved.
Li Z.-G.,Siping Center Hospital |
Ben L.,Tuohua Biological Technology Co |
Han Y.,Tuohua Biological Technology Co |
Bi W.-W.,Tuohua Biological Technology Co
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: The relationship between placing time after stem cell preparation and cell survival is the basis of safety and effectiveness for the clinical application. OBJECTIVE: To observe effects of different preservation solutions and different storage time on survival rate of umbilical cord-derived mesenchymal stem cells, and to provide important evidence for identifying effectiveness of stem cells. METHODS: Umbilical cord-derived mesenchymal stem cells were selected to prepare stem cell preparation, which was preserved in physiological saline, medium, medium+physiological saline, physiological saline containing epidermal growth factor, and medium containing low molecular heparin calcium suspension. Cold closet was selected for imitating cellular transport conditions. Samples were obtained at 0, 6, 12, 13, 24, 30, 36, 42 and 43 hours. Total cell number and cell survival rate were detected. RESULTS AND CONCLUSION: The difference in cell number and survival rate was not great within 24 hours in each group. Twenty-four hours later, total cell number and survival rate were better in the medium and medium containing low molecular heparin calcium groups than in the physiological saline containing epidermal growth factor, physiological saline, nnd medium+physiological saline groups. These findings suggest that nfter stem cell preparation, the cell survival rate can reach more than 90% within 24 hours under refriierated transport conditions. Nutritional ingredients and proper pH value of preservation solution can make the cell survival rate increased greatly. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research, All Right Reserved.
Sun L.,Shenyang University |
Tang L.,Siping Center Hospital |
Xu Y.,Siping Center Hospital |
Wang S.,Siping Center Hospital |
And 2 more authors.
Respirology | Year: 2010
Background and objective: Carbocysteine (S-carboxymethylcysteine) is a mucoactive drug with in vitro free radical scavenging and anti-inflammatory properties. Several clinical trials have indicated that carbocysteine reduces exacerbation rates in COPD. In the present study, the effect of carbocysteine on the airway load of Haemophilus influenzae was assessed in rats chronically exposed to cigarette smoke (CS). In addition, the effects of carbocysteine on airway mucus hypersecretion and mucociliary clearance (MCC) associated with the adherence and clearance of H. influenzae were investigated. Methods: Wistar rats were randomly divided into control, carbocysteine vehicle, CS exposure and carbocysteine treatment groups. After 12 weeks, rats were selected for quantitative inoculation of H. influenzae. BAL fluid and lungs were collected aseptically after 3 h for quantitative culture of H. influenzae. MCC was measured by quantifying the clearance of 99mTc-Sc. Goblet cell metaplasia and the presence of mucoid matter were evaluated by Alcian blue/periodic acid-Schiff staining. Mucin 5AC (Muc5AC) expression was detected by western blotting and real-time reverse transcription-PCR. Results: Exposure to CS increased airway H. influenzae load, aggravated mucus hypersecretion and delayed MCC. Treatment with carbocysteine decreased airway H. influenzae load, and attenuated airway mucus hypersecretion, with improved MCC associated with adherence and clearance of H. influenzae. Conclusions: These results suggest that carbocysteine may be beneficial in patients with COPD by increasing the clearance of bacteria and decreasing bacterial load. © 2010 Asian Pacific Society of Respirology.
Zhang X.-Y.,Siping Center Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: At present, the clinical application of amplified neural stem cells in the treatment of nervous system diseases is still in exploratory stage. OBJECTIVE: To investigate efficacy and safety of neural stem cell transplantation for the treatment of Parkinson's disease. METHODS: The embryonic brain tissues aged 12 weeks were obtained for proliferation in vitro, then the brain tissues (5×106 -2×107) were prepared into the 4-6 mL suspension. The suspension was injected into 30 cases of Parkinson's disease through lumbar puncture and carotid injection, once a week, continuously for 3 weeks. Unified Parkinson's disease rating scale, Hoehn-Yahr scale and Schwab & England Activities of Daily Living Scale scoring were performed prior to and after transplantation. Blood and urine routine, liver function, renal function and adverse reactions were detected and evaluated prior to and after transplantation. RESUTLS AND CONCLUSION: The 12-week-old embryos cultured neural stem cells could be stably amplified in vitro with the capacities of differentiation into neurons, astrocytes and oligodendrocytes. The follow-up results showed that neural stem cell transplantation could effectively control the pathological process of Parkinson's disease, restore the damaged brain function, and improve the patient's neurological function without significant complications. It is feasible and effective to use the in vitro long-term amplified human neural stem cell transplantation in the treatment of Parkinson's disease.
Liu Y.-H.,Shenyang University |
Cai A.-L.,Shenyang University |
Jiang H.-X.,Siping Center Hospital |
Han B.,Shenyang University |
And 2 more authors.
Chinese Journal of Medical Imaging Technology | Year: 2010
Objective: To observe the value of prenatal ultrasound in the diagnosis of congenital esophageal atresia (EA). Methods: Systematic multiplanar ultrasonographic scanning were performed in 22 fetuses with suspected EA. Scan of the coronal plane and sagittal plane of the neck and upper mediastinum were included and image features of esophageal pouch were observed. Results: Esophageal pouch was demonstrated in 11 fetuses, among which EA was confirmed in 9 fetuses after delivery, while other 2 fetuses were lost during following-up. Esophageal pouch was not demonstrated in 11 fetuses, and all were postpartum confirmed normal neonates. The earliest gestational age of pouch visualization was 26 weeks. Conclusion: Prenatal ultrasonography has important value in the diagnosis of EA. Multiple views scanning of the neck and upper mediastinum is helpful to detect esophageal pouch, which can increase the detection rate of EA.