Time filter

Source Type

Zhang S.,Chinese Academy of Agricultural Sciences | Tan B.,Chinese Academy of Agricultural Sciences | Ding Y.,Inner Mongolia Agricultural University | Wang F.,Chinese Academy of Agricultural Sciences | And 5 more authors.
Virology Journal | Year: 2014

Background: Bovine viral diarrhea virus (BVDV) is a pathogen found worldwide in calves. It can cause significant economic losses in agriculture. Many BVDV strains have been isolated in China. However, the pathogenesis and complete gene characteristics of BVDV isolate have yet not been reported in China. Here, a BVDV isolate was isolated and its pathogenesis and complete genome were studied. Results: A new isolate of bovine viral diarrhea virus, named JL-1, was isolated from the spleen of a sick cow with diarrhea using MDBK cell culture. The complete genome of JL-1 is 12,276 nucleotides and contains a 5′-UTR of 382 nucleotides, a 3′-UTR of 188 nucleotides, and a large ORF encoding a polyprotein consisting of 3,901 amino acids. Genomic comparison and phylogenetic analyses of complete genomic sequence clearly showed that JL-1 fell into the BVDV-1b subtype. The result of pathogenesis of JL-1 strain showed that all infected calves developed clinical signs of elevated rectal temperatures, decreased leucopenia, and viral discharge. Viral antigen was detected in infected animal tissues using immunohistochemistry. Animals in the mock were normal. These results demonstrated that BVDV JL-1 was a virulent strain. Conclusions: This is the first study to report the pathogenesis and complete gene characterization of the BVDV strain in China. This report may set a good foundation for further study of BVDV in China. © 2014 Zhang et al.; licensee BioMed Central Ltd.


Wang W.,Institute of Special Economic Animal and Plant Science | Wang W.,Sinovet Beijing Biotechnology Co. | Shi X.,Sinovet Beijing Biotechnology Co. | Tong Q.,Sinovet Beijing Biotechnology Co. | And 5 more authors.
Virology Journal | Year: 2014

Background: Bovine viral diarrhea virus (BVDV) is one of the most important pathogens in cattle. Previously, BVDV sub-genotypes of 1b, 1c, 1d, and 1 m were detected in China. However, isolation of BVDV type 1a from cattle has not been reported in China. In 2010, twenty nasal swabs and blood samples were collected from the cattle suspected BVDV infection in Henan province, China. A BVDV isolate was isolated using cell culture, and the pathogenesis of the virus isolate was studied. Methods. Virus isolation was performed on MDBK cells. The virus identification was conducted by RT-PCR, neutralization test and immunofluorescence assay. In order to determine the genotype of the newly isolated virus, the 5′ un-translated region (5′UTR) of the virus isolate was cloned, sequenced and phylogenetically analyzed. To evaluate the virulence of the virus isolate, four BVDV sero-negative calves were intranasally inoculated with the virus suspension. Rectal temperatures and clinical signs were recorded daily. Blood samples were analyzed for changes in white blood cell counts, and tissue samples were taken for histopathology analysis. Results: A new isolate of bovine viral diarrhea virus (BVDV), named HN01, was isolated from the nasal swabs using MDBK cell culture. The HN01 strain caused cytopathic effect (CPE) in MDBK cell cultures after two passages. The virus specifically reacted to BVDV1-specific monoclonal antibody in an immunofluorescence assay. A fragment of 288 bp of genome from this isolate was amplified by the RT-PCR. Phylogenetic analysis of 5′UTR indicated that the virus was BVDV 1a. In the pathogenesis study, four calves experimentally infected with the BVDV strain developed depression, cough and other clinical signs. Calves showed high temperature over 40°C, and white blood cell counts dropped more than 40%. Conclusions: A new subgenotype 1a strain of BVDV was firstly isolated from dairy cattle in China. The experimental infection showed that the virus was moderate pathogenic to cattle and can be used as a BVDV challenge virus to evaluate the efficacy of BVDV vaccines in the target animals. © 2014 Wang et al.; licensee BioMed Central Ltd.


Wang W.,Institute of Special Economic Animal and Plant Science | Wang W.,Sinovet Beijing Biotechnology Co. | Shi X.,Sinovet Beijing Biotechnology Co. | Wu Y.,Sinovet Beijing Biotechnology Co. | And 5 more authors.
Veterinary Immunology and Immunopathology | Year: 2014

Bovine viral diarrhea virus (BVDV) 1a and 1b strains are the predominant subgenotypes in China. Because of the genetic and antigenic variability among different BVDV strains, a vaccine effective in one region may fail to protect against infections caused by different virus strains in another region. No BVDV vaccine developed with the predominant strains in China are available. In this study, the immunogenicity of an inactivated Chinese BVDV 1a NM01 vaccine strain was evaluated by challenging with a Chinese BVDV 1b JL strain. Ten 2-4-month-old calves were intramuscularly vaccinated with a single dose of the vaccine strain and boosted with same dose three weeks after the first vaccination, with five mock immunized calves serving as a control group. The average titer of neutralization antibody to BVDV 1a and BVDV 1b of immunized calves reached 1:410 and 1:96, respectively, at 21 days post the second vaccination. Twenty-one days post the second vaccination, all calves were challenged with strain JL. The clinical signs, such as the temperature and leukopenia of the immunized calves and viral shedding, were significantly less than the mock immunized calves after challenging with the virulent BVDV 1b strain, indicating that the BVDV 1a vaccine strain elicited efficacious protection against the endemic BVDV 1b strain in China. To the best of our knowledge, this is the first report of an inactivated BVDV vaccine which demonstrated effective cross-protection against BVDV type 1b infection in China. © 2014.


Wang W.,Institute of Special Economic Animal and Plant Science | Wang W.,Sinovet Beijing Biotechnology Co. | Shi X.,Sinovet Beijing Biotechnology Co. | Chen C.,Institute of Special Economic Animal and Plant Science | And 2 more authors.
Virus Genes | Year: 2014

In January 2013, several clinical signs of cattle with diarrhea, cough, nasal discharge, and fever were reported in Jilin province, China. One virus named SD1301 was isolated and identified. Complete genome of the virus is 12258nt in length and contains a 5′UTR, one open reading frame encoding a polyprotein of 3,897 amino acids and a 3′UTR. Phylogenetic analysis of 5′UTR, Npro, E1 and E2 gene demonstrated the virus belonged to BVDV 2b, and genetically related to the BVDV strain Hokudai-Lab/09 from Japan in 2010. This bovine viral diarrhea virus displays a unique genetic signature with 27-nucleotide deletion in the 5′UTR, which is similar to the bovine viral diarrhea virus C413 (AF002227). This was the first confirmed isolation of ncp BVDV2b circulating in bovine herd of China. © 2014 Springer Science+Business Media New York.


PubMed | Chinese Academy of Agricultural Sciences, Sinovet Beijing Biotechnology Co., Jilin Agricultural University and Jiangyan Animal Health Inspection Institute
Type: | Journal: Archives of virology | Year: 2016

Several biological processes as well as infectious agents, physiological or environmental stress, and perturbed antioxidant response can promote oxidative stress. Oxidative stress usually happens when cells are exposed to more electrically charged reactive oxygen species (ROS) such as H


PubMed | Chinese Academy of Agricultural Sciences, Sinovet Beijing Biotechnology Co. and Jiangyan Animal Health Inspection Institute
Type: Journal Article | Journal: BMC veterinary research | Year: 2016

Porcine reproductive and respiratory syndrome (PRRS) remains a major threat to swine industry all over the world. The aim of this study was to investigate the mechanism of pathogenesis and immune responses caused by a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV).All piglets experimentally infected with a HP-PRRSV TJ strain virus developed typical clinical signs of PRRS. The percentages of CD3These results clearly indicated that HP-PRRSV TJ strain infection would activate host humoral immune response at the early period post infection and cause severe pathological damages on lungs and inhibit cellular immune response after infection.


Zhang H.,Chinese Academy of Agricultural Sciences | Zhang H.,Sinovet Beijing Biotechnology Co. | Zhang Z.,Sinovet Beijing Biotechnology Co. | Zhang Z.,Jinan University | And 6 more authors.
Archives of Virology | Year: 2015

In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 106.125 TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC2.0 automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16–24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain. © 2015, Springer-Verlag Wien.


Objective: To develop a one-step RT-PCR method for identification of wild virus strain and gene-deleted attenuated vaccine virus strain TJM-F92 of porcine reproductive and respiratory syndrome virus (PRRSV). Methods: A pair of primers were designed according to the nsp2 gene sequences of highly pathogenic PRRSV TJ strain and gene-deleted attenuated vaccine virus strain TJM-F92, based on which a one-step RT-PCR method for identification of various wild and gene-deleted attenuated strains of PRRSV was developed and verified for specificity, sensitiveness, sensitivity and reproducibility. Results: The gene fragment at a length of 1 100 bp was amplified from TJ strain, while that at a length of 740 bp from TJM-F92 strain, indicating that the two strains were identified correctly. The developed one-step RT-PCR method showed high sensitiveness, by which PRRSV-TJ vaccine strain at 101 TCID 50/ml was detected. Both the coincidence rates of determination results of known positive and negative serum samples of piglets were 100%, indicating high sensitivity of the method. The method also showed high reproducibility. Conclusion: The developed one-step RT-PCR method might be used for clinical identification of wild PRRSV infection and PPRSV vaccination in pigs inoculated by vaccine prepared with TJM-F92 strain, which was helpful to evaluation of immune effect of vaccine and early diagnosis of wild PRRSV infection.


Leng X.,Institute of Special Wild Economic Animal and Plant Science | Leng X.,Institute of Special Wild Economic Animal and Plant science | Li Z.,Institute of Special Wild Economic Animal and Plant Science | Li Z.,Institute of Special Wild Economic Animal and Plant science | And 4 more authors.
Clinical and Vaccine Immunology | Year: 2012

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is characterized by high fever and high mortality in pigs of all ages and has severely affected the pork industry of China in the last few years. An attenuated HP-PRRSV strain, TJM, was obtained by passaging HP-PRRSV strain TJ on MARC-145 cells for 92 passages. Porcine reproductive and respiratory syndrome virus (PRRSV)- and antibody-free pigs were inoculated intramuscularly with TJM (105.0 50% tissue culture infective doses [TCID50]) and challenged at 28, 60, 120, and 180 days postimmunization (dpi). The results showed that 5/5, 5/5, 5/5, and 4/5 immunized pigs were protected from the lethal challenge and did not develop fever and clinical diseases at each challenge, respectively. Compared to control pigs, vaccinated pigs showed much milder pathological lesions and gained significantly more weight (P < 0.01). Sequence analysis of different passages of strain TJ showed that the attenuation resulted in a deletion of a continuous 120 amino acids (aa), in addition to the discontinuous 30-aa deletion in the nsp2 region. The analysis also demonstrated that the 120-aa deletion was genetically stable in vivo. These results suggested that HP-PRRSV TJM was efficacious against a lethal challenge with a virulent HP-PRRSV strain, and effective protection could last at least 4 months. Therefore, strain TJM is a good candidate for an efficacious modified live virus vaccine as well as a useful molecular marker vaccine against HP-PRRSV. Copyright © 2012, American Society for Microbiology. All Rights Reserved.


Patent
Sinovet Beijing Biotechnology Co. | Date: 2012-05-25

The present disclosure provides vaccine compositions comprising a PRRSV vaccine and a second porcine vaccine, which are substantially free from immuno-inhibition against each other. The second porcine virus vaccine can be CSFV and/or PRV. The preparation methods for the vaccines and the formulations are also provided. The vaccine compositions provided herein confer protective immunity to pigs against porcine reproductive and respiratory syndrome, classical swine fever, and/or pseudorabies.

Loading Sinovet Beijing Biotechnology Co. collaborators
Loading Sinovet Beijing Biotechnology Co. collaborators