Sinovet Beijing Biotechnology Co.

Beijing, China

Sinovet Beijing Biotechnology Co.

Beijing, China
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Liu X.,Chinese Academy of Agricultural Sciences | Wang F.-X.,Chinese Academy of Agricultural Sciences | Zhu H.-W.,Chinese Academy of Agricultural Sciences | Sun N.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Archives of Virology | Year: 2016

Porcine circovirus type 2 (PCV2) is an important emerging pathogen that has been causatively associated with multifactorial disease syndromes in pigs and other species. It has a worldwide distribution and causes significant economic losses in the swine industry. Its genome is dynamically evolving through recombination and mutation, and the circulating genotypes of PCV2 strains in Asia are PCV2a, PCV2b and PCV2d. In this study, 12 PCV isolates were evaluated and identified by amplification, sequencing, and phylogenetic analysis, and the results revealed a new monophyletic group of PCV in China. More importantly, three of these isolates shared high homology within the ORF1 region with a strain of genotype PCV2c that was detected only in Denmark. Phylogeographic analysis of these isolates suggested that the isolates may have arisen in Denmark and that they were then transported to China. © 2016, Springer-Verlag Wien.


Wang H.,Shandong Sinder Technology Co. | Guo S.,Longcheng Middle School | Li Z.,Sinovet Beijing Biotechnology Co. | Xu X.,Jiangyan Animal Health Inspection Institute | And 2 more authors.
Archives of Virology | Year: 2017

H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension culture conditions for the avian influenza H9N2 virus (Re-2 strain) in Madin-Darby Canine Kidney (MDCK) cells, we studied the culture conditions for cell growth and proliferation parameters for H9N2 virus replication. MDCK cells were successfully cultured in suspension, from a small scale to industrial levels of production, with passage time and initial cell density being optimized. The influence of pH on the culture process in the reactor has been discussed and the process parameters for industrial production were explored via amplification of the 650L reactor. Subsequently, we cultivated cells at high cell density and harvested high amounts of virus, reaching 10log2 (1:1024). Furthermore an animal experiment was conducted to detect antibody. Compared to the chicken embryo virus vaccine, virus cultured from MDCK suspension cells can produce a higher amount of antibodies. The suspension culture process is simple and cost efficient, thus providing a solid foundation for the realization of large-scale avian influenza vaccine production. © 2017 Springer-Verlag GmbH Austria


Li Z.,Chinese Academy of Agricultural Sciences | Li Z.,Sinovet Beijing Biotechnology Co. | Xu X.,Jiangyan Animal Health Inspection Institute | Leng X.,Jilin Agricultural University | And 4 more authors.
Archives of Virology | Year: 2017

Several biological processes as well as infectious agents, physiological or environmental stress, and perturbed antioxidant response can promote oxidative stress. Oxidative stress usually happens when cells are exposed to more electrically charged reactive oxygen species (ROS) such as H2O2 or O2 −. ROS are well known for being both beneficial and deleterious. Recent studies have indicated that ROS are deleterious to cells, leading to programmed cell death (PCD) at high concentrations. At low concentrations, however, ROS can act as signaling molecules in a variety of cellular processes. In this review, we present an update of our current understanding of the role and regulation of reactive oxygen species in various viral infections, cellular signaling pathways and immune responses. We then discuss how the antioxidant defense system acts as an antiviral effector to limit cell damage. © 2016, Springer-Verlag Wien.


Zhang S.,Chinese Academy of Agricultural Sciences | Tan B.,Chinese Academy of Agricultural Sciences | Ding Y.,Inner Mongolia Agricultural University | Wang F.,Chinese Academy of Agricultural Sciences | And 5 more authors.
Virology Journal | Year: 2014

Background: Bovine viral diarrhea virus (BVDV) is a pathogen found worldwide in calves. It can cause significant economic losses in agriculture. Many BVDV strains have been isolated in China. However, the pathogenesis and complete gene characteristics of BVDV isolate have yet not been reported in China. Here, a BVDV isolate was isolated and its pathogenesis and complete genome were studied. Results: A new isolate of bovine viral diarrhea virus, named JL-1, was isolated from the spleen of a sick cow with diarrhea using MDBK cell culture. The complete genome of JL-1 is 12,276 nucleotides and contains a 5′-UTR of 382 nucleotides, a 3′-UTR of 188 nucleotides, and a large ORF encoding a polyprotein consisting of 3,901 amino acids. Genomic comparison and phylogenetic analyses of complete genomic sequence clearly showed that JL-1 fell into the BVDV-1b subtype. The result of pathogenesis of JL-1 strain showed that all infected calves developed clinical signs of elevated rectal temperatures, decreased leucopenia, and viral discharge. Viral antigen was detected in infected animal tissues using immunohistochemistry. Animals in the mock were normal. These results demonstrated that BVDV JL-1 was a virulent strain. Conclusions: This is the first study to report the pathogenesis and complete gene characterization of the BVDV strain in China. This report may set a good foundation for further study of BVDV in China. © 2014 Zhang et al.; licensee BioMed Central Ltd.


Wang W.,Institute of Special Economic Animal and Plant Science | Wang W.,Sinovet Beijing Biotechnology Co. | Shi X.,Sinovet Beijing Biotechnology Co. | Wu Y.,Sinovet Beijing Biotechnology Co. | And 5 more authors.
Veterinary Immunology and Immunopathology | Year: 2014

Bovine viral diarrhea virus (BVDV) 1a and 1b strains are the predominant subgenotypes in China. Because of the genetic and antigenic variability among different BVDV strains, a vaccine effective in one region may fail to protect against infections caused by different virus strains in another region. No BVDV vaccine developed with the predominant strains in China are available. In this study, the immunogenicity of an inactivated Chinese BVDV 1a NM01 vaccine strain was evaluated by challenging with a Chinese BVDV 1b JL strain. Ten 2-4-month-old calves were intramuscularly vaccinated with a single dose of the vaccine strain and boosted with same dose three weeks after the first vaccination, with five mock immunized calves serving as a control group. The average titer of neutralization antibody to BVDV 1a and BVDV 1b of immunized calves reached 1:410 and 1:96, respectively, at 21 days post the second vaccination. Twenty-one days post the second vaccination, all calves were challenged with strain JL. The clinical signs, such as the temperature and leukopenia of the immunized calves and viral shedding, were significantly less than the mock immunized calves after challenging with the virulent BVDV 1b strain, indicating that the BVDV 1a vaccine strain elicited efficacious protection against the endemic BVDV 1b strain in China. To the best of our knowledge, this is the first report of an inactivated BVDV vaccine which demonstrated effective cross-protection against BVDV type 1b infection in China. © 2014.


PubMed | Chinese Academy of Agricultural Sciences, Sinovet Beijing Biotechnology Co., Jilin Agricultural University and Jiangyan Animal Health Inspection Institute
Type: | Journal: Archives of virology | Year: 2016

Several biological processes as well as infectious agents, physiological or environmental stress, and perturbed antioxidant response can promote oxidative stress. Oxidative stress usually happens when cells are exposed to more electrically charged reactive oxygen species (ROS) such as H


PubMed | Chinese Academy of Agricultural Sciences, Sinovet Beijing Biotechnology Co. and Jiangyan Animal Health Inspection Institute
Type: Journal Article | Journal: BMC veterinary research | Year: 2016

Porcine reproductive and respiratory syndrome (PRRS) remains a major threat to swine industry all over the world. The aim of this study was to investigate the mechanism of pathogenesis and immune responses caused by a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV).All piglets experimentally infected with a HP-PRRSV TJ strain virus developed typical clinical signs of PRRS. The percentages of CD3These results clearly indicated that HP-PRRSV TJ strain infection would activate host humoral immune response at the early period post infection and cause severe pathological damages on lungs and inhibit cellular immune response after infection.


Objective: To develop a one-step RT-PCR method for identification of wild virus strain and gene-deleted attenuated vaccine virus strain TJM-F92 of porcine reproductive and respiratory syndrome virus (PRRSV). Methods: A pair of primers were designed according to the nsp2 gene sequences of highly pathogenic PRRSV TJ strain and gene-deleted attenuated vaccine virus strain TJM-F92, based on which a one-step RT-PCR method for identification of various wild and gene-deleted attenuated strains of PRRSV was developed and verified for specificity, sensitiveness, sensitivity and reproducibility. Results: The gene fragment at a length of 1 100 bp was amplified from TJ strain, while that at a length of 740 bp from TJM-F92 strain, indicating that the two strains were identified correctly. The developed one-step RT-PCR method showed high sensitiveness, by which PRRSV-TJ vaccine strain at 101 TCID 50/ml was detected. Both the coincidence rates of determination results of known positive and negative serum samples of piglets were 100%, indicating high sensitivity of the method. The method also showed high reproducibility. Conclusion: The developed one-step RT-PCR method might be used for clinical identification of wild PRRSV infection and PPRSV vaccination in pigs inoculated by vaccine prepared with TJM-F92 strain, which was helpful to evaluation of immune effect of vaccine and early diagnosis of wild PRRSV infection.


Leng X.,Institute of Special Wild Economic Animal and Plant Science | Leng X.,Institute of Special Wild Economic Animal and Plant science | Li Z.,Institute of Special Wild Economic Animal and Plant Science | Li Z.,Institute of Special Wild Economic Animal and Plant science | And 4 more authors.
Clinical and Vaccine Immunology | Year: 2012

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is characterized by high fever and high mortality in pigs of all ages and has severely affected the pork industry of China in the last few years. An attenuated HP-PRRSV strain, TJM, was obtained by passaging HP-PRRSV strain TJ on MARC-145 cells for 92 passages. Porcine reproductive and respiratory syndrome virus (PRRSV)- and antibody-free pigs were inoculated intramuscularly with TJM (105.0 50% tissue culture infective doses [TCID50]) and challenged at 28, 60, 120, and 180 days postimmunization (dpi). The results showed that 5/5, 5/5, 5/5, and 4/5 immunized pigs were protected from the lethal challenge and did not develop fever and clinical diseases at each challenge, respectively. Compared to control pigs, vaccinated pigs showed much milder pathological lesions and gained significantly more weight (P < 0.01). Sequence analysis of different passages of strain TJ showed that the attenuation resulted in a deletion of a continuous 120 amino acids (aa), in addition to the discontinuous 30-aa deletion in the nsp2 region. The analysis also demonstrated that the 120-aa deletion was genetically stable in vivo. These results suggested that HP-PRRSV TJM was efficacious against a lethal challenge with a virulent HP-PRRSV strain, and effective protection could last at least 4 months. Therefore, strain TJM is a good candidate for an efficacious modified live virus vaccine as well as a useful molecular marker vaccine against HP-PRRSV. Copyright © 2012, American Society for Microbiology. All Rights Reserved.


Patent
Sinovet Beijing Biotechnology Co. | Date: 2012-05-25

The present disclosure provides vaccine compositions comprising a PRRSV vaccine and a second porcine vaccine, which are substantially free from immuno-inhibition against each other. The second porcine virus vaccine can be CSFV and/or PRV. The preparation methods for the vaccines and the formulations are also provided. The vaccine compositions provided herein confer protective immunity to pigs against porcine reproductive and respiratory syndrome, classical swine fever, and/or pseudorabies.

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