Sinopharm Wuhan Pharmaceutical Industry Design Institute

Wuhan, China

Sinopharm Wuhan Pharmaceutical Industry Design Institute

Wuhan, China

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Ran J.,Tianjin University | Jia S.,Tianjin University | Liu Y.,Tianjin University | Zhang W.,Tianjin University | And 2 more authors.
Bioresource Technology | Year: 2010

In this paper, we introduced a polydiol (mixture of 1,2-propanediol, 1,3-propanediol, and 2,3-butanediol) to improve the covalent crosslinking adsorption process of immobilized catalase onto chitosan beads. The adsorption behavior was investigated by means of adsorption kinetics and adsorption isotherm. The protein content in crosslinking agent required for approximately 45 min to reach the relative equilibrium, and the protein content in solution of the control group and the pretreated group were 6.63 lg/mL and 6.20 lg/mL, respectively. The maximum catalase adsorption capacity of the control group and the pretreated group were observed as 23.118 lg/g and 25.688 lg/g at pH 7.0, respectively. Temperature profiles showed that 40 °C was the ideal temperature for active domain of catalase, and the relative activity of pretreated group was 1.12 times higher than that of the control group. The Km value of the control group (67 mM) was higher than that of the pretreated group (54 mM). Thermal stability, operational stability, and the effect of surfactant on catalase adsorption were also explored in this study.


Zhang C.-Y.,Huazhong University of Science and Technology | Zhang C.-Y.,SINOPHARM Wuhan Pharmaceutical Industry Design Institute | Dong Y.-S.,Huazhong University of Science and Technology | Li Y.-L.,Huazhong University of Science and Technology | And 4 more authors.
Huaxue Gongcheng/Chemical Engineering (China) | Year: 2013

The volumetric oxygen transfer coefficient of plant cells suspension cultures in the bioreactor differs substantially from the values predicted from aqueous solution or sulfite oxidation. Therefore, there is a real need to have a reliable on-line method for measuring volumetric oxygen transfer coefficient during cultures. A new method for determination of the volumetric oxygen transfer coefficient was proposed based on the dissolved oxygen concentration-time during the aeration following nongassing. The volumetric oxygen transfer coefficient of Taxus media cells suspension cultures in a 10 L bioreactor was measured. The influence of the power input per unit volume and the superficial velocity on the volumetric oxygen transfer coefficient was investigated. An experiential correlation among the volumetric oxygen transfer coefficient KLa, the power input per unit volume N3D2, and the superficial velocity vS in the bioreactor were established. The changes of the volumetric oxygen transfer coefficient, the oxygen uptake rate and the specific oxygen consumption rate for suspension cultures of Taxus media cells in a 10 L bioreactor were researched, and the approaches of the determination of the oxygen uptake rate, the specific oxygen consumption rate and the oxygen saturation concentration in the broth were discussed. The result shows that the effect of aeration amount on the volumetric oxygen transfer coefficient is much higher than that of agitation speed; with the increase of cell biomass, the volumetric oxygen transfer coefficient decreases, the oxygen uptake rate increases, and the specific oxygen consumption rate first increases and then decreases. The results are helpful to the operation control, reactor design and amplification of Taxus media cell suspension cultures in the bioreactor.


Zhang C.-Y.,Huazhong University of Science and Technology | Zhang C.-Y.,Key Laboratory of Molecular Biophysics of Ministry of Education | Zhang C.-Y.,SINOPHARM Wuhan Pharmaceutical Industry Design Institute | Dong Y.-S.,Huazhong University of Science and Technology | And 9 more authors.
Biochemical Engineering Journal | Year: 2013

For a better understanding of the simulation, optimization, and process control in cell cultures, good kinetic models are necessary for large scale plant cell culture. In this paper, the systematic kinetics of taxol production by Taxus media cell suspension cultures in a stirred 15-L bioreactor under substrate-sufficient conditions and the absence of inducer intervention were studied. A kinetic model of cell growth was established by logistic equation, and kinetic unstructured models of substrate consumption, product synthesis and rheological behavior were constituted, which incorporated energy spilling. These models were verified by comparing the simulation results with those obtained experimentally. These results showed that energy spilling was a key factor that must be considered in constructing unstructured kinetic models of Taxus media cell suspension cultures in a stirred bioreactor under substrate-sufficient conditions. Besides, an optimized operation measure of decreasing energy spilling was proposed. An increase of 17.64% in cell biomass and 14.88% in taxol concentration were obtained when the strategy of limiting added carbon several times was experimentally implemented in a 15-L bioreactor. Results demonstrated that these established models should be helpful in the process prediction and operation optimization to guide the production and amplification of Taxus media cell suspension cultures in a bioreactor. © 2012 Elsevier B.V.


Zhang C.-Y.,Huazhong University of Science and Technology | Zhang C.-Y.,Ministry of Education Key Laboratory of Molecular Biophysics | Zhang C.-Y.,SINOPHARM Wuhan Pharmaceutical Industry Design Institute | Dong Y.-S.,Huazhong University of Science and Technology | And 7 more authors.
Wuhan Ligong Daxue Xuebao/Journal of Wuhan University of Technology | Year: 2012

In this paper, rheological properties of Taxus media cells suspension cultures in a bioreactor were investigated. The rheological equations were obtained by regression analysis. The results demonstrated that the suspension cultures broth of Taxus media cells at different fermentation time exhibited pseudoplastic non-Newtonian fluid, the broth apparent viscosity increased linearly with the increase in cell concentration and there was the linear functional relations between the apparent viscosity and the percentage aggregation. The pseudoplasticity and consistency coefficient of the broth increased with time from lag phase to stationary phase of cell growth. Subsequently, both appeared to decrease. At the same time the flow behavior index decreased with time from lag phase to stationary phase and increased during decline phase of cell growth. The results should be helpful in operation control, reactor design and amplification of Taxus media cell suspension cultures in a bioreactor.

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