Entity

Time filter

Source Type


Zhang S.,Gansu Agricultural University | Zhang S.,Key Laboratory of Grassland Ecosystems | Zhang S.,Pratacultural Engineering Laboratory of Gansu Province | Zhang S.,Sino Us Centers For Grazingland Ecosystems Sustainability | And 6 more authors.
BioControl | Year: 2014

Trichoderma longibrachiatum can be used for the control of Heterodera avenae in crops, but the effectiveness and possible mechanisms are unknown. Here we determined the efficacy and the mechanism responsible for the nematode control in spring wheat (Triticum aestivum L.). Wheat seedlings inoculated with T. longibrachiatum at the concentrations from 1.5 × 104 to 1.5 × 108 spores ml-1 significantly increased plant height, root length, and plant biomass; decreased H. avenae infection in both rhizospheric soil and roots; and enhanced chlorophyll content, root activity, and the specific activities of resistance-related enzymes (peroxidase, polyphenol oxidase and phenylalanine ammonia lyase), compared to the control. Those reactions occurred soon after T. longibrachiatum inoculation and the effect reached the maximum 7-9 days after inoculation. Promoting competitive plant growth and inducing enzyme-trigged resistance serve as the main mechanism responsible for T. longibrachiatum against H. avenae. T. longibrachiatum can be considered an effective biocontrol agent against H. avenae in wheat. © 2014 International Organization for Biological Control (IOBC). Source


Zhang S.,Gansu Agricultural University | Zhang S.,Key Laboratory of Grassland Ecosystems | Zhang S.,Pratacultural Engineering Laboratory of Gansu Province | Zhang S.,Sino Us Centers For Grazingland Ecosystems Sustainability | And 10 more authors.
Biological Control | Year: 2014

Heterodera avenae is a devastating plant pathogen that causes significant yield losses in many crops, but there is a lack of scientific information whether this pathogen can be controlled effectively using biocontrol agents. Here we determined the parasitic and lethal effects of Trichoderma longibrachiatum against H. avenae and the possible mechanism involved in this action. Both in vitro and greenhouse experiments were conducted. In vitro, T. longibrachiatum at the concentrations of 1.5×104 to 1.5×108spores per ml had a strong parasitic and lethal effect on the cysts of H. avenae, with the concentration of 1.5×108spores per ml having >90% parasitism 18days after treatments. In greenhouse, T. longibrachiatum inoculation decreased H. avenae infection in wheat (Triticum aestivum) significantly. Observations with microscopes revealed that after mutual recognition with cysts, the spore of T. longibrachiatum germinated with a large number of hyphae, and reproduced rapidly on the surface of cysts. Meanwhile, the cysts surface became uneven, with some cysts producing vacuoles, and the others splitting. Finally the cysts were dissolved by the metabolite of T. longibrachiatum. Chitinase activity increased in the culture filtrates of T. longibrachiatum and reached the maximum 4days after inoculation in the medium supplemented with colloidal chitin (1.02U/minperml) and nematode cysts (0.78U/minperml). The parasitism and inhibition of cysts through the increased extracellular chitinase activity serves as the main mechanism with which T. longibrachiatum against H. avenae. In conclusion, T. longibrachiatum has a great potential to be used as a biocontrol agent against H. avenae. © 2014 Elsevier Inc. Source


Zhang S.,Gansu Agricultural University | Zhang S.,Key Laboratory of Grassland Ecosystems | Zhang S.,Pratacultural Engineering Laboratory of Gansu Province | Zhang S.,Sino Us Centers For Grazingland Ecosystems Sustainability | And 5 more authors.
Applied Soil Ecology | Year: 2015

Meloidogyne incognita is one of the most important soil-borne and plant parasitic pathogens in cucumber worldwide. An ideal strategy is to develop and use effective and environmentally-friendly bio-control agents to control the plant parasitic pathogen M. incognita. This study determined the biocontrol efficacy of Trichoderma longibrachiatum, a fungal species native to China, against M. incognita. In vitro, T. longibrachiatum at the concentrations of 1.5×105 to 1.5×107 conidiaml-1 had a strong lethal and parasitic effect on the second stage juveniles of M. incognita, with the concentration of 1.5×107 conidiaml-1 inhibited and parasitized the second stage juveniles >88% 14 days after treatments. Microscope observations revealed that after the mutual recognition with the second stage juveniles, the conidia of T. longibrachiatum adhered or parasitized on the surface of second stage juveniles, germinated with a large number of hyphae and penetrated the integument, and was reproduced on the surface of second stage juveniles. Meanwhile, the surface of second stage juveniles became deformed and some were completely dissolved by the metabolite of T. longibrachiatum. The maximum protease activity of T. longibrachiatum was 3.35Umin-1ml-1 at the 5th day after the treatment of second stage juveniles. In greenhouse experiments, the concentrations of T. longibrachiatum ranging from 1.5×105 to 1.5×107 conidiaml-1 all decreased M. incognita infection and increased plant height, root length, shoot and root fresh weights in cucumber significantly compared to the control. T. longibrachiatum can be considered to be a promising bio-control agent against M. incognita with a high efficacy. © 2015. Source

Discover hidden collaborations