Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province

Taian, China

Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province

Taian, China
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Hao H.,Shandong Agricultural University | Hao H.,Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province | Li C.,Shandong Agricultural University | Li C.,Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province | And 25 more authors.
Veterinary Microbiology | Year: 2014

To further investigate the airborne infection mechanism of Marek's disease virus (MDV), a MDV aerosol infection model was established, and the generation, transmission and infectiosity of MDV aerosols were monitored in this study. Two positive/negative pressure isolators, in which SPF chickens were raised, were connected with a closed conduit. Two repetitive trials, Trial 1 (T1) and Trial 2 (T2) were carried out for objective assessment. Air samples were collected using the AGI-30 sampler. Viral DNA in air samples and feather follicle samples were detected using real-time quantitative PCR (QRT-PCR). MDV in air and blood samples was detected by indirect immunofluorescence assay (IFA). In chickens of isolator A (MDV inoculation group), MDV was detected in feather follicles in 100% of the tested chickens at 6 days post inoculation (dpi) in both trials; and MDV was isolated from blood samples at 9-10dpi. MDV DNA was detected in air samples from isolator A at 12dpi in T1 and 14dpi in T2 and concentration of aerosolized MDV DNA was peaked at 3.84×106copies/m3 air at 40dpi in T1, and 6.17×105copies/m3 air at 38dpi in T2, respectively. Infectious MDV (cell culture) was isolated from isolator A at 17 in T1 and 19dpi in T2, respectively. MDV aerosol in Isolator B was almost same as isolator A. Viremia was detected in isolator B at 26-30dpi. The incidence of viremia in isolator B reached 70% at 3 months post inoculation. These results demonstrated that infected chicken could discharge virus, the MDV could form aerosols and infect neighboring chickens. Understanding the mechanism of generation and infection of MDV aerosols is helpful to prevent and control MD. © 2014 Elsevier B.V.


Bai Y.,Shandong Agricultural University | Zhao X.,Shandong Agricultural University | Qi C.,Central Hospital of Taian City | Wang L.,Shandong Agricultural University | And 6 more authors.
Human and Experimental Toxicology | Year: 2014

Chromium picolinate (CrPic), which is used as a nutritional supplement and to treat type 2 diabetes, has gained much attention because of its cytotoxicity. This study evaluated the effects of CrPic on the viability of the chick embryo fibroblast (CEF) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, morphological detection, and flow cytometry. The results show that lower concentrations of CrPic (8 and 16 μM) did not damage CEF viability (p > 0.05). However, higher CrPic concentrations (400 and 600 μM) indicated a highly significant effect on the production of intracellular reactive oxygen species, alteration of mitochondrial membrane potential, intracellular calcium ion concentration, and the apoptosis rate (p < 0.01), contrary to lower CrPic concentrations (8 and 16 μM) and control group. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrPic was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress-induced mitochondrial dysfunction was apoptotic death. © 2014 The Author(s).


Miao Z.,Shandong Agricultural University | Miao Z.,Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province | Cai Y.,Shandong Agricultural University | Cai Y.,Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province | And 6 more authors.
Aerobiologia | Year: 2012

To accurately quantify airborne Aspergillus fumigatus (A. fumigatus) spores in rabbit houses, the real-time polymerase chain reaction (real-time PCR) and culture-based counting method (CCM) were employed to determine the airborne A. fumigatus spore concentrations. The results showed that, of the three rabbit houses (A, B, and C), the average concentrations of airborne A. fumigatus spores determined by real-time PCR were 3.0 × 10 3, 3.3 × 10 3, and 1.5 × 10 3 spores/m 3 air, respectively, while those determined by CCM were 2.5 × 10 2, 2.8 × 10 2, and 1.1 × 10 2 colony-forming unit/m 3 air (CFU/m 3 air), respectively, i.e., the former concentration was 12-14 times higher than the latter one. Therefore, the conventional CCM underestimated the concentrations of airborne fungal spores, and it is insufficient to determine the microbial aerosol concentration and evaluate the health risk only using CCM. © Springer Science+Business Media B.V. 2011.

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