Bai Y.,Shandong Agricultural University |
Zhao X.,Shandong Agricultural University |
Qi C.,Central Hospital of Taian City |
Wang L.,Shandong Agricultural University |
And 6 more authors.
Human and Experimental Toxicology | Year: 2014
Chromium picolinate (CrPic), which is used as a nutritional supplement and to treat type 2 diabetes, has gained much attention because of its cytotoxicity. This study evaluated the effects of CrPic on the viability of the chick embryo fibroblast (CEF) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, morphological detection, and flow cytometry. The results show that lower concentrations of CrPic (8 and 16 μM) did not damage CEF viability (p > 0.05). However, higher CrPic concentrations (400 and 600 μM) indicated a highly significant effect on the production of intracellular reactive oxygen species, alteration of mitochondrial membrane potential, intracellular calcium ion concentration, and the apoptosis rate (p < 0.01), contrary to lower CrPic concentrations (8 and 16 μM) and control group. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrPic was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress-induced mitochondrial dysfunction was apoptotic death. © 2014 The Author(s).
Miao Z.,Shandong Agricultural University |
Miao Z.,Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province |
Cai Y.,Shandong Agricultural University |
Cai Y.,Sino German Cooperative Research Center for Zoonosis of Animal Origin of Shandong Province |
And 6 more authors.
Aerobiologia | Year: 2012
To accurately quantify airborne Aspergillus fumigatus (A. fumigatus) spores in rabbit houses, the real-time polymerase chain reaction (real-time PCR) and culture-based counting method (CCM) were employed to determine the airborne A. fumigatus spore concentrations. The results showed that, of the three rabbit houses (A, B, and C), the average concentrations of airborne A. fumigatus spores determined by real-time PCR were 3.0 × 10 3, 3.3 × 10 3, and 1.5 × 10 3 spores/m 3 air, respectively, while those determined by CCM were 2.5 × 10 2, 2.8 × 10 2, and 1.1 × 10 2 colony-forming unit/m 3 air (CFU/m 3 air), respectively, i.e., the former concentration was 12-14 times higher than the latter one. Therefore, the conventional CCM underestimated the concentrations of airborne fungal spores, and it is insufficient to determine the microbial aerosol concentration and evaluate the health risk only using CCM. © Springer Science+Business Media B.V. 2011.