Sinisa Stankovic Institute for Biological Research

Belgrade, Serbia

Sinisa Stankovic Institute for Biological Research

Belgrade, Serbia
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Wu B.,University of Hamburg | Wu B.,Huazhong University of Science and Technology | Matic D.,University of Hamburg | Matic D.,Sinisa Stankovic Institute for Biological Research | And 6 more authors.
Experimental Neurology | Year: 2012

It is widely accepted that the immune system plays important functional roles in regeneration after injury to the spinal cord. Immune response towards injury involves a complex interplay of immune system cells, such as neutrophils, macrophages and microglia, T- and B-lymphocytes. We investigated the influence of the lymphocyte component of the immune system on the locomotor outcome of severe spinal cord injury in a genetic mouse model of immune suppression. Transgenic mice lacking mature T- and B-lymphocytes due to the recombination activating gene 2 gene deletion (RAG2-/- mice) were subjected to severe compression of the lower thoracic spinal cord, with the wild-type mice of the same inbred background serving as controls. According to both the Basso Mouse Scale score and single frame motion analysis, the RAG2-/- mice showed improved recovery in comparison to control mice at six weeks after injury. Better locomotor function was associated with enhanced catecholaminergic and cholinergic reinnervation of the spinal cord caudal to injury and increased axonal regrowth/sprouting at the site of injury. Myelination of axons in the ventral column measured as g-ratio was more extensive in RAG2-/- than in control mice 6. weeks after injury. Additionally, the number of microglia/macrophages was decreased in the lumbar spinal cord of RAG2-/- mice after injury, whereas the number of astrocytes was increased compared with controls. We conclude that T- and B-lymphocytes restrict functional recovery from spinal cord injury by increasing numbers of microglia/macrophages as well as decreasing axonal sprouting and myelination. © 2012 Elsevier Inc.

Stevanovic I.D.,Military Medical Academy | Jovanovic M.D.,Military Medical Academy | Colic M.,Military Medical Academy | Jelenkovic A.,Sinisa Stankovic Institute for Biological Research | And 2 more authors.
Brain Research Bulletin | Year: 2010

The present experiment was carried out to determine the effectiveness of nitric oxide synthase inhibitors: 7-nitroindazole and aminoguanidine in modulating the toxicity of aluminium chloride on acetylcholine esterase activity, as well as behavioural and morphological changes of Wistar rats. For biochemical analysis the animals were killed 10 min, 3 h, 3 days and 30 days after the treatment and forebrain cortex, striatum, basal forebrain and hippocampus were removed. The biochemical changes observed in neuronal tissues show that nitric oxide synthase inhibitors exert as protective action in aluminium chloride-treated animals. In the present study, active avoidance learning was significantly impaired after aluminium chloride injection, while pretreatment with nitric oxide synthase inhibitors prevented the behavioural deficits caused between 26th and 30th day after intrahippocampal application of neurotoxin. Our data suggest that aluminium may cause learning and memory deficits, while the treatment with specific nitric oxide synthase inhibitors may prevent learning and memory deficits caused by aluminium chloride. We have also applied immunohistochemical techniques to identify neuronal- and inducible-nitric oxide synthase expression 30 days after aluminium chloride and nitric oxide synthase inhibitors injections. Our data suggest that 7-nitroindazole and aminoguanidine can be effective in the protection of toxicity induced by aluminium chloride. © 2010 Elsevier Inc. All rights reserved.

Stevovic S.,Union University of Serbia | Mikovilovic V.S.,Union University of Serbia | Calic-Dragosavac D.,Sinisa Stankovic Institute for Biological Research
African Journal of Biotechnology | Year: 2010

The aim of this work was to define the correlation between heavy metal (mercury, lead, cadmium, chrome and nickel) concentration changes (in soil, leaf, stem, root and essential oil in Tansy) and type and distance from source pollution. The concentration was monitored on different locations: 1) Chemical industry pan evo (with accident situation); 2) Pan evo industrial zone; 3) highway; 4) Ada Ciganlija recreation zone and 5) Top ider park. Mercury (Hg) concentration was analyzed as a function of time, starting from accident situation from 1999 to 2008. Hg had maximum concentration of 131 200 mg/kg at a soil depth of 0 - 15 cm. After three, six and nine years, linear accident Hg concentrations in soil decreased from 85 400 via 41 060 to 106.0 mg/kg. Mercury concentration which results in the location where accident occurred showed that 6 years was necessary for concentration to drop below the limits and for revitalization of standard industrial Tansy vegetation. Concentrations of Hg in the Tansy plants from contaminated site were 5, 10, 100 and 200 times greater than in industrial zone, highway, Ada Ciganlija and Top ider, respectively. The highest amount of lead (Pb) was in leaves (14.1 mg/kg) and in essential oil (0.7 mg/kg) of Tansy near the highway. However, Pb concentration in soil and plant was decreased with square of highway distance. © 2010 Academic Journals.

Lenhardt M.,Sinisa Stankovic Institute for Biological Research | Hegedis A.,Serbian Institute for Multidisciplinary Research | Maletin S.,University of Novi Sad | Cirkovic M.,University of Novi Sad
Reviews in Fish Biology and Fisheries | Year: 2011

A total of 22 fish species have been introduced into the inland waters of Serbia, either intentionally or accidentally. This paper provides a summary of data concerning time and reason of introduction, mode of expansion, degree of acclimatization, impact on native fish and estimated area of recent distribution. Four of the non-native fish species currently occupy more than 51% of Serbian territory while 5 of them occupy between 21-50% of territory. This paper reviews impacts of introduced freshwater fish in Serbia based on collected data. © 2010 Springer Science+Business Media B.V.

Cirovic D.,University of Belgrade | Rakovic M.,Sinisa Stankovic Institute for Biological Research | Milenkovic M.,Sinisa Stankovic Institute for Biological Research | Paunovic M.,Natural History Museum
Biological Invasions | Year: 2011

Since its first appearance in the second half of the 1980s, the Small Indian Mongoose (Herpestes auropunctatus) has spread along the entire coastline of Montenegro, from the Croatian border in the north to the Albanian border in the south. Its present range includes an area of 447.3 km2 and is limited to the narrow coastal zone up to 300 m above sea level. The distribution of this species generally corresponds to the thick Mediterranean maquis and garigue vegetation. The Small Indian Mongoose spread to the territory of Montenegro through natural immigration from neighboring Croatia, where it was introduced in the past. The southward expansion along the coastline of the Adriatic Sea has increased the previously known European range by about 150 km in the last 20 years. The appearance of this species in the "Adriatic triangle", one of the most important European centers herpetofauna diversity, could lead to catastrophic consequences for this fauna. © 2010 Springer Science+Business Media B.V.

Miljkovic D.,Sinisa Stankovic Institute for Biological Research | Timotijevic G.,University of Belgrade | Stojkovic M.M.,University of Belgrade
FEBS Letters | Year: 2011

Astrocytes are the most abundant cell population within the CNS of mammals. Their glial role is perfectly performed in the healthy CNS as they support functions of neurons. The omnipresence of astrocytes throughout the white and grey matter and their intimate relation with blood vessels of the CNS, as well as numerous immunity-related actions that these cells are capable of, imply that astrocytes should have a prominent role in neuroinflammatory disorders, such as multiple sclerosis (MS). The role of astrocytes in MS is rather ambiguous, as they have the capacity to both stimulate and restrain neuroinflammation and tissue destruction. In this paper we present some of the proved and the proposed functions of astrocytes in neuroinflammation and discuss the effect of MS therapeutics on astrocytes. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Tucic B.,Sinisa Stankovic Institute for Biological Research | Miljkovic D.,Sinisa Stankovic Institute for Biological Research
Plant Species Biology | Year: 2010

Fluctuating asymmetry (FA), the subtle, random difference between the left and right side of bilateral structures, has often been used as a tool for evaluating developmental instability (DI) in natural populations subjected to environmental stresses. A general assumption underlying these studies is that the level of DI should increase with stress intensity. We examined the level of floral FA in six natural populations of Iris pumila experiencing sun-exposed (more stressful) and shaded (less stressful) environmental conditions. We used two single-trait indices (size-dependent FA1 and size-scaled FA8a) and one multi-trait index (FA17) to assess the FA levels in three floral traits: fall width, standard width and style branch width. Although floral FA was present in all FAs and appeared to be greater in plants from full sunlight than in those beneath vegetation canopy, only the FA17 index detected a significant FA-stress association. Although the FA1 index had no statistical power to reveal differences in the floral FA at any of the hierarchical levels studied, between alternative light habitats, among populations from comparable environmental conditions, among individual clones within each population or between different floral organs of a single flower, the FA8a index detected significant between-trait variation in the degree of floral FA within the same Iris individuals. © 2010 The Authors. Journal compilation © 2010 The Society for the Study of Species Biology.

Velickovic M.V.,Sinisa Stankovic Institute for Biological Research
Periodicum Biologorum | Year: 2010

Background and Purpose: Developmental stability (DS) or homeostasis refers to the ability of an individual to produce a consistent phenotype in a given environment. Reduced DS can result from a wide variety of environmentally (or genetically) induced perturbations. The main aim of the presented paper is to highlight the importance of the differences in ability of Tilia cordata leaves to buffer their development under contrasting environmental conditions and points to the concept that developmental stability is character specific. Materials and Methods: Three different techniques were performed in this study: fluctuation asymmetry (FA) and leaf size as integrative measures of environmental stress during leaf developmental processes and within - plant variance in leaf morphology, presented as coefficient of morphological variation (CV). The study tested the hypothesis that the population from a chronically polluted area would express greater developmental instability in leaf traits. Two bilateral, linear dimensions on each leaf: leafwidth (LW) and lobe length (LL) were analyzed. Results: The three different measures of developmental stability all showed a trend for T. cordata leaves in the polluted area to be developmentally less stable than leaves from the reference area. Leaves in the reference area were significantly larger compared with those from the polluted site. Although leaves tend to be larger on the outside of a tree's crown, the pattern found here was the reverse. Both, outside and inside leaves from the polluted area had significantly higher FAs than leaves from the same position sampled in the reference area for both traits.Within-tree variance assessed as CV showed that LL was a more variable measure than LW. Moreover, the data suggest that LL is under more selective pressure to adapt to current environmental conditions than LW. Conclusions: Obtained data suggest that T. cordata leaves may represent a reliable indicator for developmental stability evaluation studies based on an assay using a combination of end-points. Furthermore, my results high-lighted the differences in ability of leaf morphometric characters to buffer their development under contrasting environmental conditions.

Jaric I.,Serbian Institute for Multidisciplinary Research | Ebenhard T.,Swedish University of Agricultural Sciences | Lenhardt M.,Sinisa Stankovic Institute for Biological Research
Reviews in Fish Biology and Fisheries | Year: 2010

Populations of six sturgeon species in the Danube River (beluga, Russian sturgeon, stellate sturgeon, sterlet, ship sturgeon and Atlantic sturgeon) have experienced severe decline during the last several decades, mostly due to the unsustainable fishery, river fragmentation and water pollution. Present lack of knowledge on basic sturgeon demography, life history and relative effects of different negative factors is further hindering implementation of efficient policy and management measures. In the present study, population viability analysis in a Vortex simulation model has been conducted in order to assess the state of the six Danube sturgeon species, their future risk of extinction and to determine the most suitable conservation and management measures. Population viability analysis has revealed a large sensitivity of the Danube sturgeon populations to changes in the natural mortality, fecundity, age at maturity and spawning frequency. It was also confirmed that the sturgeons are highly susceptible to even moderate levels of commercial fishery, and that their recovery is a multi-decadal affair. Stocking with adult individuals was shown to produce considerably greater effect on population persistence than stocking with juveniles, but the latter approach is probably still preferable since it avoids many inherent problems of aquaculture cultivation. This study represents the first population viability analysis of the Danube sturgeons. © 2009 Springer Science+Business Media B.V.

Jaric I.,Serbian Institute for Multidisciplinary Research | Visnjic-Jeftic Z.,Serbian Institute for Multidisciplinary Research | Cvijanovic G.,Serbian Institute for Multidisciplinary Research | Gacic Z.,Serbian Institute for Multidisciplinary Research | And 3 more authors.
Microchemical Journal | Year: 2011

The accumulation of heavy metals in fish has been extensively studied and well documented. However, the research has been mainly focused on the muscle tissue, while the distribution patterns among other tissues, such as liver and gills, have been mostly neglected. Within the present study, the concentrations of Ag, Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Se, Sr, Zn and Li in the muscle, gills, liver and intestine of the sterlet (Acipenser ruthenus) from the Danube River have been assessed by using the inductively-coupled plasma optical spectrometry. The analysis has revealed a high degree of differential accumulation of the studied heavy metals and trace elements in the tissues of the Danube sterlet. Liver appeared to be the main heavy metal storage tissue, while the lowest levels of analyzed metals were found in muscles. Heavy metal concentrations in the muscle were at acceptable levels for human consumption, except partly for cadmium. We conclude that, in order to enable a more efficient control of contaminants in fish products, there is an apparent need to resolve the problem of lack of legal limits for many metals in European legislation. © 2010 Elsevier B.V.

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