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Murumkar P.R.,Sinhgad Institute of Pharmacy Narhe | Sharma M.K.,Sinhgad Institute of Pharmacy Narhe | Shinde A.C.,Sinhgad Institute of Pharmacy Narhe | Bothara K.G.,Sinhgad Institute of Pharmacy Narhe
Medicinal Chemistry Research | Year: 2013

A series of pyrrolidine-based tartrate diamides having selective tumor necrosis factor-α converting enzyme (TACE) inhibitory activity was selected for the three-dimensional quantitative structure-activity relationship (3D-QSAR) studies. Total 76 compounds were selected by considering a high deviation in the biological activity and structural variations. The quality and predictive power of 3D-QSAR, comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) models for the atom-based, centroid/atom-based, data-based alignments were performed. Various models were developed with the help of these alignments. The best model was developed with data-based alignment. The optimal predictive CoMFA model was obtained with cross-validated r 2 = 0.53 with six component, non-cross-validated r 2 = 0.94, standard error of estimates 0.23, F-value = 121.98 and optimal CoMSIA model was obtained with cross-validated r 2 = 0.53 with five components, non-cross-validated r 2 = 0.93, standard error of estimates = 0.24 and F-value = 138.83. These models also showed the best test set prediction with predictive r 2 value of 0.65 and 0.73, respectively. Thus, on the basis of predictive power COMSIA model appeared to be the best one. The statistical parameters from these models indicate that the data are being well fitted and also have high predictive ability. Moreover, the resulting 3D-CoMFA/CoMSIA contour maps provide useful guidance for designing of highly active TACE inhibitors. © 2012 Springer Science+Business Media New York.


Gawande V.,Sinhgad Institute of Pharmacy Narhe | Singh A.K.,Sinhgad Institute of Pharmacy Narhe | Rathor P.,Sinhgad Institute of Pharmacy Narhe | Damania D.,Sinhgad Institute of Pharmacy Narhe
Journal of Analytical Chemistry | Year: 2016

The objective of the present study was to develop and validate a rapid and simple stability indicating analytical method for estimating Ilaprazole. Ilaprazole was subjected to different stress conditions prescribed by International Conference on Harmonization (ICH) such as hydrolysis, oxidation, photolytic and dry heat degradation conditions. The drug was very susceptible to degradation under hydrolysis and photolytic conditions, less susceptible to oxidation and stable under dry heat degradation condition. An acceptable separation of drug and its degradants was achieved by using a C-18 column and mobile phase composed of ammonium acetate buffer (pH 3.2)—acetonitrile (55: 45, v/v). Flow rate was 1 mL/min and detection wavelength was set at 303 nm. Retention time of drug was found to be 6.6 min and analysis can be completed within 10 min. The method was validated with respect to linearity, precision, accuracy, robustness, LOD and LOQ as per ICH. The method was linear (R2 = 0.996) in the range of 2.5–250 μg/mL. The recovery was in the range from 99.2–100.2%. © 2016, Pleiades Publishing, Ltd.

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