Sinhgad Institute of Pharmacy

Pune, India

Sinhgad Institute of Pharmacy

Pune, India
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Shiradkarb M.R.,Lupin Research Park | Botharac K.G.,Sinhgad Institute of Pharmacy
Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry | Year: 2011

The reaction of ethyl 2-amino-4-methylthiazole-5-carboxylate 1 with acetic anhydride followed by reaction with hydrazine hydrate yields the ethyl 2-acetamido-4-methylthiazole-5- carboxylate 2 and N-[5-(hydrazinecarbonyl)-4- methylthiazol-2- yl]acetamide 3, respectively. The compound 3 on further reaction with alcoholic potassium hydroxide-carbon disulphide followed by cyclization with hydrazine hydrate gives N-[5-(4-amino-5- mercapto-4H-1,2,4- triazol-3-yl)-4-methylthiazol-2-yl]acetamide 5. The compound 5 is then condensed with different aromatic aldehydes to offer Schiff bases 6a-h. The Schiff bases on cyclization with chloroacetyl chloride in presence of triethylamine as catalyst furnish the azetidin-2-one 7a-h. The compounds are synthesized in good yield and the chemical structures of the compounds are elucidated from their IR, 1H NMR, and elemental analysis. All the synthesized compounds have been screened for their antimicrobial activity.

Zhiang W.,CAS Institute of Microbiology | Kokare C.,Sinhgad Institute of Pharmacy
International Journal of Biological Macromolecules | Year: 2014

A Nocardiopsis sp. stain B2 with an ability to produce stable α-amylase was isolated from marine sediments. The characterization of microorganism was done by biochemical tests and 16S rDNA sequencing. The α-amylase was purified by gel filtration chromatography by using sephadex G-75. The molecular mass of the amylase was found to be 45kDa by SDS-PAGE and gel filtration chromatography. The isolated α-amylase was immobilized by ionotropic gelation technique using gellan gum (GG). These microspheres were spherical with average particle size of 375.62±21.76 to 492.54±32.18μm. The entrapment efficiency of these α-amylase loaded GG microspheres was found 74.76±1.32 to 87.64±1.52%. Characterization of α-amylase-gellan gum microspheres was confirmed using FTIR and SEM analysis. The in vitro amylase release kinetic have been studied by various mathematical models that follow the Korsmeyer-Peppas model (R2=0.9804-0.9831) with anomalous (non-Fickian) diffusion release mechanism. © 2014.

Kokare C.,Sinhgad Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2013

An extracellular alkaline protease from marine Streptomyces sp. D1 was isolated and characterized. The protease was purified using ammonium sulfate precipitation method, followed by dialysis and Sephadex G-100 gel chromatography, with an 8.82-fold increase in specific activity and a 12.8% recovery. The molecular weight was found to be 28 kDa, determined by SDS-PAGE. The purified enzyme was completely inhibited by phenylmethylsulphonyl fluoride, indicating presence of a serine protease. Protease enzyme was found to have maximum activity at 450C and pH 10, respectively. The enzyme was stable in pH range 8-10 and temperature 45-600C. The enzyme was found to be halotolerant, retains 92.5% of its initial activity after 6 h. The alkaline protease was found stable in presence of non-ionic surfactant (1% Triton X-100) retains 81.5% residual activity after 48 h and 92.57 % residual activity after 6 h with oxidizing agent (1% H2O2). The protease inhibited the growth of several pathogenic organisms such as B. subtilis, E. coli, P. aeruginosa and S. aureus. Enzyme also exhibited good hair removal activity from goat skin. These unique properties make this protease an ideal choice in food, pharmaceutical, leather and detergent industries.

Chakraborty S.,Bharati Vidyapeeth Deemed University | Khopade A.,Bharati Vidyapeeth Deemed University | Biao R.,CAS Institute of Microbiology | Jian W.,CAS Institute of Microbiology | And 6 more authors.
Journal of Molecular Catalysis B: Enzymatic | Year: 2011

A halopalkaliphilic marine Saccharopolyspora sp. stain A9 with an ability to produce surfactants, oxidant and detergent stable α-amylase was isolated from marine sediments collected from west coast of India. The α-amylase from strain A9 was purified to homogeneity with the aid of ammonium sulfate precipitation and gel filtration chromatography by using Sephadex G-75, insoluble corn starch and sephacryl S-100 column, with a 39.01-fold increase in specific activity. SDS-PAGE and zymogram activity staining showed a single band equal to molecular mass of 66 kDa. Enzyme was found to be stable in presence of wide range of NaCl concentration with maximum activity found at 11% (w/v) of NaCl. Enzyme showed remarkable stability towards laboratory surfactants, detergents and oxidants. Glucose, maltose and maltotriose were the main end product of starch hydrolysis, indicating it is α-amylase. © 2010 Elsevier B.V. All rights reserved.

Aswar U.,Sinhgad Institute of Pharmacy | Gurav M.,Sinhgad Institute of Pharmacy | More G.,Sinhgad Institute of Pharmacy | Rashed K.,National Research Center of Egypt | Aswar M.,Sinhgad Institute of Pharmacy
Journal of Integrative Medicine | Year: 2014

Objective Solanum xanthocarpum Schrad. & Wendl. (Solanaceae) is present in many Ayurveda compound formulations including Chavanaprasha and Dasamoolarishta. The whole plant is used in conditions such as inflammation, constipation and promoting conception in females. In the present study, we carried out different tests to evaluate the effect of aqueous extract of Solanum xanthocarpum (SXE) in postmenopausal syndrome. Methods The study was carried out in bilaterally ovariectomized one-month-old Wistar rats (40-50 g). Bilaterally ovariectomized (OVX) Wistar rats were divided into four groups (n=6) receiving different treatments, consisting of a vehicle (distilled water), aqueous extract of Solanum xanthocarpum at two different doses (200 and 400 mg/kg) administered orally daily for 90 d and standard drug β estradiol at a dose of 1 mg/kg administered subcutaneously biweekly for 90 d. Estrogenic activity was assessed by vaginal cornification, sexual behavior, serum estradiol and uterine weight to body weight ratio. Antiosteoporotic activity was assessed on the basis of biomechanical and biochemical parameters followed by histopathological studies, and antidepressant activity was assessed by forced swim test. Results SXE showed presence of steroids. At the dose of 200 mg/kg, it significantly improved all the parameters of sexual behavior (P<0.01), caused vaginal cornification, and increased serum estradiol and uterine weight (P<0.01). It also significantly improved all the parameters of bone strength as well as depression (P<0.01). Histopathology of bones confirmed the above findings. Conclusion The study indicated that SXE may provide an effective treatment in the prevention of postmenopausal symptoms. © 2014 Journal of Integrative Medicine Editorial Office. E-edition published by Elsevier (Singapore) Pte Ltd. All rights reserved.

Khopade A.,Bharati Vidyapeeth Deemed University | Biao R.,CAS Institute of Microbiology | Liu X.,CAS Institute of Microbiology | Mahadik K.,Bharati Vidyapeeth Deemed University | And 3 more authors.
Desalination | Year: 2012

A potential biosurfactant producing strain, marine Nocardiopsis B4 was isolated from the West coast of India. Culture conditions involving variations in carbon and nitrogen sources were examined at constant pH, temperature and revolutions per min (rpm), with the aim of increasing productivity in the process. The biosurfactant production was followed by measuring the surface tension, emulsification assay and emulsifying index E24. Enhanced biosurfactant production was carried out using olive oil as the carbon source and phenyl alanine as the nitrogen source. The maximum production of the biosurfactant by Nocardiopsis occurred at a C/N ratio of 2:1 and the optimized bioprocess condition was pH 7.0, temperature 30° C and salt concentration 3%. The production of the biosurfactant was growth dependent. The surface tension was reduced up to 29. mN/m as well as the emulsification index E24 was 80% in 6 to 9. days. Properties of the biosurfactant that was separated by acid precipitation were investigated. The biosurfactant activity was stable at high temperature, a wide range of pH and salt concentrations thus, indicating its application in bioremediation, food, pharmaceutical and cosmetics industries. © 2011 Elsevier B.V.

Khopade A.,Bharati Vidyapeeth Deemed University | Ren B.,CAS Institute of Microbiology | Liu X.-Y.,CAS Institute of Microbiology | Mahadik K.,Bharati Vidyapeeth Deemed University | And 3 more authors.
Journal of Colloid and Interface Science | Year: 2012

The present study demonstrates the production and properties of a biosurfactant isolated from marine Streptomyces species B3. The production of the biosurfactant was found to be higher in medium containing sucrose and lower in the medium containing glycerol. Yeast extract was the best nitrogen source for the production of the biosurfactant. The isolated biosurfactant reduced the surface tension of water to 29. mN/m. The purified biosurfactant was shown critical micelle concentrations of 110. mg/l. The emulsifying activity and stability of the biosurfactant was investigated at different salinities, pH, and temperature. The biosurfactant was effective at very low concentrations over a wide range of temperature, pH, and salt concentration. The purified biosurfactant was shown strong antimicrobial activity. The biosurfactant was produced from the marine Streptomyces sp. using non-hydrocarbon substrates such as sucrose that was readily available and not required extensive purification procedure. Streptomyces species B3 can be used for microbially enhanced oil recovery process. © 2011 Elsevier Inc..

Aswar U.M.,Sinhgad Institute of Pharmacy | Kandhare A.D.,Indus Biotech Private Ltd 1 | Mohan V.,Indus Biotech Private Ltd 1 | Thakurdesai P.A.,Indus Biotech Private Ltd 1
Phytotherapy Research | Year: 2015

The objective of the present work was to evaluate anti-allergic effects of intranasal administration of type-A procynidines polyphenols (TAPP) based standardized hydroalcoholic extract of Cinnamomum zeylanicum bark (TAPP-CZ) in ovalbumin (OVA)-induced experimental allergic rhinitis (AR) in BALB/c mice. Sixty male BALB/c mice were divided into six groups of ten each (G1-G6). The mice from G1 were nonsensitized and maintained as normal group. Remaining mice (G2-G6) were sensitized with OVA (500 μL solution, intraperitoneal) on alternate days for 13 days and had twice daily intranasal treatment from day 14-21 as follows: G2 (AR control) received saline, G3 (positive control, XLY) received xylometazoline (0.5 mg/mL, 20 μL/nostril) and G4-G6 received TAPP-CZ (3, 10 and 30 μg/kg in nostril), respectively. On day 21, mice were challenged with OVA (5 μL/nostril, 5% solution) and assessments (nasal signs, biochemical and histopathological) were performed. Treatment with TAPP-CZ (10 and 30 μg/kg in nostril) showed significant attenuation in OVA-induced alterations of the nasal (number of nasal rubbing and sneezing), biochemical markers (serum IgE and histamine), haematological, morphological (relative organ weight of spleen and lung) and histopathological (nasal mucosa and spleen) parameters. In conclusion, TAPP-CZ showed anti-allergic efficacy in animal model of AR. Copyright © 2014 John Wiley & Sons, Ltd. Copyright © 2014 John Wiley & Sons, Ltd.

Bobade V.,Bharati Vidyapeeth Deemed University | Bodhankar S.L.,Bharati Vidyapeeth Deemed University | Aswar U.,Sinhgad Institute of Pharmacy | Vishwaraman M.,Indus Biotech Private Ltd | Thakurdesai P.,Indus Biotech Private Ltd
Chinese Journal of Natural Medicines | Year: 2015

The present study aimed at evaluation of prophylactic efficacy and possible mechanisms of asiaticoside (AS) based standardized extract of Centella asiatica (L.) Urban leaves (INDCA) in animal models of migraine. The effects of oral and intranasal (i.n.) pretreatment of INDCA (acute and 7-days subacute) were evaluated against nitroglycerine (NTG, 10 mg·kg-1, i.p.) and bradykinin (BK, 10 μg, intra-arterial) induced hyperalgesia in rats. Tail flick latencies (from 0 to 240 min) post-NTG treatment and the number of vocalizations post-BK treatment were recorded as a measure of hyperalgesia. Separate groups of rats for negative (Normal) and positive (sumatriptan, 42 mg·kg-1, s.c.) controls were included. The interaction of INDCA with selective 5-HT1A, 5-HT1B, and 5-HT1D receptor antagonists (NAN-190, Isamoltane hemifumarate, and BRL-15572 respectively) against NTG-induced hyperalgesia was also evaluated. Acute and sub-acute pre-treatment of INDCA [10 and 30 mg·kg-1 (oral) and 100 μg/rat (i.n.) showed significant anti-nociception activity, and reversal of the NTG-induced hyperalgesia and brain 5-HT concentration decline. Oral pre-treatment with INDCA (30 mg·kg-1, 7 d) showed significant reduction in the number of vocalization. The anti-nociceptive effects of INDCA were blocked by 5-HT1A and 5-HT1B but not 5-HT1D receptor antagonists. In conclusion, INDCA demonstrated promising anti-nociceptive effects in animal models of migraine, probably through 5-HT1A/1B medicated action. © 2015 China Pharmaceutical University.

Datar P.A.,Sinhgad Institute of Pharmacy
Journal of Pharmaceutical Analysis | Year: 2015

Abstract Bioanalytical methods are widely used for quantitative estimation of drugs and their metabolites in physiological matrices. These methods could be applied to studies in areas of human clinical pharmacology and toxicology. The major bioanalytical services are method development, method validation and sample analysis (method application). Various methods such as GC, LC-MS/MS, HPLC, HPTLC, micellar electrokinetic chromatography, and UFLC have been used in laboratories for the qualitative and quantitative analysis of carbamazepine in biological samples throughout all phases of clinical research and quality control. The article incorporates various reported methods developed to help analysts in choosing crucial parameters for new method development of carbamazepine and its derivatives and also enumerates metabolites, and impurities reported so far. © 2015 The Authors.

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