Palaskar S.,Sinhgad dental college and hospital
Journal of Clinical and Diagnostic Research | Year: 2010
Aims: To compare the Papanicolaou's (Pap) and May Grunwald's Giemsa (MGG) staining techniques which are done to detect micronuclei (MN) in exfoliated buccal mucosal cells in individuals with different tobacco habits. To determine the severity of human buccal cell changes which are associated with smoking and smokeless (''chewing'') tobacco (SLT). Methods and Material: A total of 45 male subjects (15 smokers, 15 smokeless tobacco users and 15 non users/ non smokers) were examined. Two cytological smears were taken from the apparently normal buccal mucosa from each individual. 45 smears (1 per individual) were wet fixed and stained with Pap and the remaining 45 smears were air dried and stained with the MGG stain. All the smears were assessed for cellularity and were scored for MN. Statistical analysis used: The ANOVA (one way analysis of variance) was used to analyse the frequency of cells with micronuclei. Bonferroni multiple comparisons were done to determine the significance (p<0.05) of the mean difference. Results: MNs were easily seen in the clear cytoplasm in the Pap smears. Regarding the MGG smears, bacteria and cell debris masked the effect of the MNs as compared to the Pap smears, where the fixative destroyed the bacteria and clearly demarcated the cell boundaries. The score of the MN frequency decreased as we moved from the smokeless tobacco chewers to the smokers and then to the non users and the non smokers. Conclusions: Pap is a better stain as compared to MGG for counting micronuclei. Smokeless tobacco chewers showed an increased number of MNs as compared to the smokers, thus laying emphasis on the greater carcinogenic potential of tobacco which was used in the chewable form.
Nagpal D.K.J.,Sinhgad Dental College and Hospital |
Prabhu P.R.,Sinhgad Dental College and Hospital |
Palaskar S.,Sinhgad Dental College and Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2013
Leiomyosarcoma (LMS) is an uncommon malignant spindle cell tumor of the head and neck region. The occurrence is particularly rare in the buccal mucosa of the oral cavity. It is a rapidly growing tumor with aggressive behavior and poor prognosis. Method: This article presents a rare case of primary leimyosarcoma of the buccal mucosa in a 35 year old female and retrospective analysis of primary oral LMS published in the English literature since past 20 years is done. Diagnosis was confirmed by immunohistochemistry profile showing positivity for vimentin, smooth muscle actin (SMA), high proliferative index displayed by Ki-67, focal positivity for pan-CK and negativity for S-100. Conclusion: Based on the presence of malignant spindle cells showing positivity for vimentin and SMA, a diagnosis of leiomyosarcoma was made.
Mittal N.,Desh Bhagat Dental College |
Palaskar S.,Sinhgad Dental College and Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2012
Background: The etiology of oral lichen planus (OLP) is not fully understood. It is generally considered to be a T-cell mediated chronic inflammatory oral mucosal disease. There is increasing evidence that chronic inflammation is linked to the diseases associated with endothelial dysfunction and is involved in the induction of aberrant angiogenesis. Aim: Our aim was to evaluate the role of angiogenesis in the pathogenesis of OLP by immunohistochemistry, using the CD34 antibody. Materials and Methods: Forty tissue sections (7 of erosive lichen planus, 18 of reticular oral lichen planus, and 15 of normal oral mucosa), were assessed for microvessel density (MVD) in five selected areas of high inflammatory infiltrate by immunohistochemistry for the expression of CD34 antibody. Results and Conclusion: The mean MVD was 44.47 in the control group (normal oral mucosa) and 97.24 in the OLP group, showing that there is increased angiogenesis in the latter. Reticular OLP had mean MVD of 84.61 and erosive OLP had mean MVD of 129.71, showing relatively greater angiogenesis in erosive OLP as compared to reticular OLP. Thus, angiogenesis can be considered to play a role in both the etiopathogenesis and the progression of OLP.
Andrade N.N.,Nair Hospital Dental College |
Raikwar K.,Sinhgad Dental College and Hospital
British Journal of Oral and Maxillofacial Surgery | Year: 2010
Teratomas of the tongue are rare, and often accompany other anomalies within the head and neck. We describe a combination of anomalies in a 6-week-old infant with teratoma and bifid tip of the tongue, severe tongue tie, and polydactyly. The teratoma was excised and the tongue tie released with no complications. © 2010 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
Shetty V.D.,Sinhgad Dental College and Hospital |
Bijle M.N.A.,Dr DY Patil Dental College and Hospital |
Patil S.,MS Ramaiah Dental College and Hospital
Journal of Contemporary Dental Practice | Year: 2013
Aim: To assess and compare the quality of life and oral health status among institutionalized elderly in Pune. Materials and methods: A cross-sectional, quantitative exploratory study was conducted in persons 60 years of age in an institutionalized elderly home. The GOHAI questionnaire was completed by a single examiner, who interviewed the patients in their local language. The oral examination was carried out according to WHO oral health survey using the DMFT-index, community periodontal index (CPI), periodontal loss of attachment (PLA), prosthetic use, and needs according to criteria established by the WHO by a calibrated examiner. Descriptive analysis was carried out using absolute and relative frequencies of the qualitative variables and means with respective standard deviations for the quantitative variables. The Student's t-test was applied to compare groups. Results: Out of the total 110 individuals interviewed for the study, 64% were males and 36% were females. Individuals with up to 20 missing teeth and individuals with more than 20 missing teeth were 20 and 80% respectively. Based on the prosthetic need, 66% individuals needed replacement of teeth by dentures in one or both arches while 34% did not need any replacement by complete denture. The mean value for physical dimension was 5.40 whereas for psychosocial dimension, pain/discomfort and behavioral dimension were 8.02, 4.58 and 7.32. Conclusion: The quality of life was found to be more favorable in individuals with less than 20 teeth missing. The quality of life among complete denture wearers in both the arches was better as compared to the quality of life among nondenture wearers. Prosthetic replacement by complete denture helps the individuals to maintain better oral health. Clinical significance: The data obtained from the present study may serve as a reference point for comparisons of the magnitude of quality-of-life indicators relating to oral health. Clinical significance: The data obtained from the present study may serve as a reference point for the magnitude of quality-of-life indicators relating to oral health.
Palaskar S.J.,Sinhgad Dental College and Hospital |
Garde J.,Sinhgad Dental College and Hospital |
Bartake A.,Sinhgad Dental College and Hospital |
Narang B.,Sinhgad Dental College and Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2014
The teratoid cyst is a rare variant of the dermoid cyst which seldom occurs in the oral cavity. If seen, they generally present as slow growing cysts of the floor of mouth, reported commonly in the 2nd and 3rd decade of life in males. Histopathologically, dermoid cyst is classified as epidermoid cyst, true dermoid cyst and teratoid cyst depending on the presence of adnexal structures and derivatives of all three germ layers. Herewith we report a rare case of teratoid cyst of the floor of the mouth, in a 2-year-old female child, which was present since birth. © 2014, Medknow. All rights reserved.
Shinde A.,Sinhgad Dental College and Hospital |
Ganu J.,Government Medical College |
Naik P.,MVPS Dr Vasantrao Pawar Medical College |
Sawant A.,Sinhgad Dental College and Hospital
Biomedical Research | Year: 2012
The exact pro-oxidant and antioxidant status in alcoholic liver disease among the chronic alcoholics is still not clear. Alcoholic liver disease (ALD) & its complications are still one of the most frequent causes of death in both developing and developed counties. The present study was conducted to add a new insight to the question, whether ethanol induces oxidative stress, change in the level of antioxidant vitamins ascorbic acid & vitamins E (non- enzymatic antioxidant parameters), trace elements Zinc & Selenium & activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx) & catalase in erythrocytes. Study was performed in 40 patients with alcoholic liver disease (study subject) & compared to 40 age & sex matched healthy subject (controls). It was observed that they was a significant increase in MDA (Malondialdehyde), SOD, GPx and significant decrease in ascorbic acid, vitamin E, zinc, selenium & catalase activity in patients with alcoholic liver disease when compared to controls. The results of our study have shown higher free radical production, as evidenced by the increased MDA & decreased levels of ascorbic acid, vitamin E, selenium & catalase activity, supporting the hypothesis that they is increased oxidative stress in patients with ALD. The increased activities of antioxidant enzymes may be a compensatory regulatory response to increased oxidative stress. The decreased antioxidative status support the hypothesis that lipid peroxidation is an important causative factor in the pathogenesis of ALD. These data clearly indicates that, antioxidant mechanisms might be impaired in these patients. These findings also provide a therotical basis for the development of novel therapeutic strategies such as antioxidant supplementation.
Garde J.B.,Sinhgad Dental College and Hospital
BMJ case reports | Year: 2012
Odontogenic keratocyst (OKC) is a unique cyst because of its locally aggressive behaviour, high recurrence rate and characteristic histological appearance. A radical surgical approach is commonly advocated with morbid and disfiguring results. This approach also presents several reconstructive obstacles especially in anterior mandible. In this case report the authors present a 25-year-old male patient with a large OKC and treatment with decompression followed by enucleation, and chemical cauterisation. This approach though demanding prolonged treatment time and postoperative follow-up decreases the morbidity to a great extent in a young individual. At the same time, it allows an opportunity for the maxillofacial surgeon to preserve the natural dentition, maintain function and safeguard cosmesis. In our case, the authors effectively achieved all the aforementioned objectives. At the same time a radical option of treatment is still left in the armoury if required at a later date.
Padalkar R.K.,Padmashree Dr Vitthalrao V Patil Foundations Medical College |
Shinde A.V.,Sinhgad Dental College and Hospital |
Patil S.M.,Padmashree Dr Vitthalrao V Patil Foundations Medical College
Biomedical Research | Year: 2012
The present study was carried out in patients with chronic kidney diseases (CKD) and type 2 diabetes mellitus to evaluate the status of lipid profile, malondialdehyde and superoxide dismutase in total 50 patients of CKD and 50 patients of type 2 diabetes mellitus and 40 healthy individuals were included as control in this study. After 12 hrs, fasting 5 ml. venous blood sample was collected, allowed to clot for half an hour and serum was separated. Lipid profile was measured by using commercially available kit of metro lab auto analyzer. Serum MDA was measured by Wilbur et al method, serum SOD levels were measured by Marklund and Marklund method. The values are expressed as mean ± SD and P value of less than 0.05 was considered as significant. The levels of total cholesterol, LDL cholesterol were decreased where as Triglcerides (TG), VLDL Cholesterol were elevated in CKD patients as compared to control group, though the values were not statistically significant (P>0.05). In type 2 diabetic patients HDL cholesterol significantly decreased (P<0.05) while other parameters of lipid profile were significantly increased (P<0.05) as compared to control group. Serum MDA and SOD were significantly higher (P<0.05) in CKD and type 2 diabetic patients, but not statistically significant as compare to control. In conclusion study shows significant lipoprotein abnormalities in CKD and type 2 diabetes mellitus patients when compared to control. High levels of MDA in CKD and diabetic patients indicating to an increased oxidative stress, the increasing SOD level trying to fight against oxidative stress.
Ramamoorth M.,Sinhgad Dental College and Hospital |
Narvekar A.,Sinhgad Dental College and Hospital
Journal of Clinical and Diagnostic Research | Year: 2015
Non-viral vectors are simple in theory but complex in practice. Apart from intra cellular and extracellular barriers, number of other challenges also needs to be overcome in order to increase the effectiveness of non-viral gene transfer. These barriers are categorized as production, formulation and storage. No one-size-fits-all solution to gene delivery, which is why in spite of various developments in liposome, polymer formulation and optimization, new compounds are constantly being proposed and investigated. In this review, we will see in detail about various types of non-viral vectors highlighting promising development and recent advances that had improved the non-viral gene transfer efficiency of translating from “Bench to bedside”. © 2015, Journal of Clinical and Diagnostic Research. All rights reserved.