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Padalkar R.K.,Padmashree Dr Vitthalrao V Patil Foundations Medical College | Shinde A.V.,Sinhgad Dental College and Hospital | Patil S.M.,Padmashree Dr Vitthalrao V Patil Foundations Medical College
Biomedical Research

The present study was carried out in patients with chronic kidney diseases (CKD) and type 2 diabetes mellitus to evaluate the status of lipid profile, malondialdehyde and superoxide dismutase in total 50 patients of CKD and 50 patients of type 2 diabetes mellitus and 40 healthy individuals were included as control in this study. After 12 hrs, fasting 5 ml. venous blood sample was collected, allowed to clot for half an hour and serum was separated. Lipid profile was measured by using commercially available kit of metro lab auto analyzer. Serum MDA was measured by Wilbur et al method, serum SOD levels were measured by Marklund and Marklund method. The values are expressed as mean ± SD and P value of less than 0.05 was considered as significant. The levels of total cholesterol, LDL cholesterol were decreased where as Triglcerides (TG), VLDL Cholesterol were elevated in CKD patients as compared to control group, though the values were not statistically significant (P>0.05). In type 2 diabetic patients HDL cholesterol significantly decreased (P<0.05) while other parameters of lipid profile were significantly increased (P<0.05) as compared to control group. Serum MDA and SOD were significantly higher (P<0.05) in CKD and type 2 diabetic patients, but not statistically significant as compare to control. In conclusion study shows significant lipoprotein abnormalities in CKD and type 2 diabetes mellitus patients when compared to control. High levels of MDA in CKD and diabetic patients indicating to an increased oxidative stress, the increasing SOD level trying to fight against oxidative stress. Source

Palaskar S.,Sinhgad Dental College and Hospital
Journal of Clinical and Diagnostic Research

Aims: To compare the Papanicolaou's (Pap) and May Grunwald's Giemsa (MGG) staining techniques which are done to detect micronuclei (MN) in exfoliated buccal mucosal cells in individuals with different tobacco habits. To determine the severity of human buccal cell changes which are associated with smoking and smokeless (''chewing'') tobacco (SLT). Methods and Material: A total of 45 male subjects (15 smokers, 15 smokeless tobacco users and 15 non users/ non smokers) were examined. Two cytological smears were taken from the apparently normal buccal mucosa from each individual. 45 smears (1 per individual) were wet fixed and stained with Pap and the remaining 45 smears were air dried and stained with the MGG stain. All the smears were assessed for cellularity and were scored for MN. Statistical analysis used: The ANOVA (one way analysis of variance) was used to analyse the frequency of cells with micronuclei. Bonferroni multiple comparisons were done to determine the significance (p<0.05) of the mean difference. Results: MNs were easily seen in the clear cytoplasm in the Pap smears. Regarding the MGG smears, bacteria and cell debris masked the effect of the MNs as compared to the Pap smears, where the fixative destroyed the bacteria and clearly demarcated the cell boundaries. The score of the MN frequency decreased as we moved from the smokeless tobacco chewers to the smokers and then to the non users and the non smokers. Conclusions: Pap is a better stain as compared to MGG for counting micronuclei. Smokeless tobacco chewers showed an increased number of MNs as compared to the smokers, thus laying emphasis on the greater carcinogenic potential of tobacco which was used in the chewable form. Source

Garde J.B.,Sinhgad Dental College and Hospital
BMJ case reports

Odontogenic keratocyst (OKC) is a unique cyst because of its locally aggressive behaviour, high recurrence rate and characteristic histological appearance. A radical surgical approach is commonly advocated with morbid and disfiguring results. This approach also presents several reconstructive obstacles especially in anterior mandible. In this case report the authors present a 25-year-old male patient with a large OKC and treatment with decompression followed by enucleation, and chemical cauterisation. This approach though demanding prolonged treatment time and postoperative follow-up decreases the morbidity to a great extent in a young individual. At the same time, it allows an opportunity for the maxillofacial surgeon to preserve the natural dentition, maintain function and safeguard cosmesis. In our case, the authors effectively achieved all the aforementioned objectives. At the same time a radical option of treatment is still left in the armoury if required at a later date. Source

Shetty V.D.,Sinhgad Dental College and Hospital | Bijle M.N.A.,Dr DY Patil Dental College and Hospital | Patil S.,MS Ramaiah Dental College and Hospital
Journal of Contemporary Dental Practice

Aim: To assess and compare the quality of life and oral health status among institutionalized elderly in Pune. Materials and methods: A cross-sectional, quantitative exploratory study was conducted in persons 60 years of age in an institutionalized elderly home. The GOHAI questionnaire was completed by a single examiner, who interviewed the patients in their local language. The oral examination was carried out according to WHO oral health survey using the DMFT-index, community periodontal index (CPI), periodontal loss of attachment (PLA), prosthetic use, and needs according to criteria established by the WHO by a calibrated examiner. Descriptive analysis was carried out using absolute and relative frequencies of the qualitative variables and means with respective standard deviations for the quantitative variables. The Student's t-test was applied to compare groups. Results: Out of the total 110 individuals interviewed for the study, 64% were males and 36% were females. Individuals with up to 20 missing teeth and individuals with more than 20 missing teeth were 20 and 80% respectively. Based on the prosthetic need, 66% individuals needed replacement of teeth by dentures in one or both arches while 34% did not need any replacement by complete denture. The mean value for physical dimension was 5.40 whereas for psychosocial dimension, pain/discomfort and behavioral dimension were 8.02, 4.58 and 7.32. Conclusion: The quality of life was found to be more favorable in individuals with less than 20 teeth missing. The quality of life among complete denture wearers in both the arches was better as compared to the quality of life among nondenture wearers. Prosthetic replacement by complete denture helps the individuals to maintain better oral health. Clinical significance: The data obtained from the present study may serve as a reference point for comparisons of the magnitude of quality-of-life indicators relating to oral health. Clinical significance: The data obtained from the present study may serve as a reference point for the magnitude of quality-of-life indicators relating to oral health. Source

Andrade N.N.,Nair Hospital Dental College | Raikwar K.,Sinhgad Dental College and Hospital
British Journal of Oral and Maxillofacial Surgery

Teratomas of the tongue are rare, and often accompany other anomalies within the head and neck. We describe a combination of anomalies in a 6-week-old infant with teratoma and bifid tip of the tongue, severe tongue tie, and polydactyly. The teratoma was excised and the tongue tie released with no complications. © 2010 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved. Source

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