Alameda, CA, United States
Alameda, CA, United States
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Patent
Singulex and The Regents Of The University Of California | Date: 2017-05-17

The invention provides methods for the sensitive detection of cardiac troponin. Such methods are used in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.


Patent
Singulex and The Regents Of The University Of California | Date: 2016-11-10

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.


Disclosed is an analyzer for determining the presence, absence or amount of an analyte in a sample, wherein the analyzer comprises: (a) an electromagnetic radiation source (301) that emits electromagnetic radiation that defines an interrogation space comprising a focused spot of a beam from the electromagnetic radiation source (301); (b) a capillary flow cell (313) for passing a sample through the interrogation space; (c) a source of motive force for moving the sample in the capillary flow cell (313); (d) an electromagnetic radiation detector (309) operatively connected to the interrogation space that detects photons emitted from the interrogation space during a plurality of bins; and (e) a processor (310) operatively connected to the detector (309), wherein the processor determines the presence, absence or amount of an analyte in a sample by:(i) determining a threshold photon value corresponding to a background signal in the interrogation space, (ii) determining the presence of a photon emitting species comprising or corresponding to the analyte in the interrogation space in each bin of the plurality of bins by identifying bins having a photon value greater than the threshold value, (iii) comparing the number of bins having a photon value greater than the threshold value to a standard curve that is generated using one or more samples of known standard concentrations.


Patent
Singulex and The Regents Of The University Of California | Date: 2015-10-23

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.


Methods, kits, and systems for determining vulnerable plaque burden in a subject. The methods involve measuring an amount of at least one biomarker in a blood sample from the subject, wherein the at least one biomarker is selected from the group consisting of vascular endothelial growth factor-A (VEGF), cardiac troponin I (cTnI), and matrix metalloproteinase-9 (MMP-9). The concentration of these biomarkers in a sample from the subject are used to determine vulnerable plaque burden in the subject.


Patent
Singulex | Date: 2015-06-29

Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnI, BNP) and vascular inflammation (IL-6, TNF, IL-17a) biomarkers provided a sensitivity of 94% for association with CHF.


The invention encompasses analyzers and analyzer systems that include a single molecule analyzer, methods of using the analyzer and analyzer systems to analyze samples, either for single molecules or for molecular complexes. The single molecule uses electromagnetic radiation that is translated through the sample to detect the presence or absence of a single molecule. The single molecule analyzer provided herein is useful for diagnostics because the analyzer detects single molecules with zero carryover between samples.


Patent
Singulex | Date: 2015-12-17

Analyzers and analyzer systems that include an analyzer for determining multiple label species, methods of using the analyzer and analyzer systems to analyze samples, are disclosed herein. The analyzer includes one or more sources of electromagnetic radiation to provide electromagnetic radiation at wavelengths within the excitation bands of one or more fluorophore species to an interrogation space that is translated through the sample to detect the presence or absence of molecules of different target analytes. The analyzer may also include one or more detectors configured to detect electromagnetic radiation emitted from the one or more fluorophore species. The analyzer for determining multiple target molecule species provided herein is useful for diagnostics because the concentration of multiple species of target molecules may be determined in a single sample and with a single system.


Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.


Disclosed are methods for diagnosing, staging, and predicting risk for developing rheumatoid arthritis and other inflammatory diseases, and methods for identifying treatment responders and non-responders.

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