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Reggio Calabria, Italy

Barbaro A.,SIMEF | Fernandez-Formoso L.,University of Santiago de Compostela | Phillips C.,University of Santiago de Compostela | Carracedo T.,University of Santiago de Compostela | Lareu M.V.,University of Santiago de Compostela
Legal Medicine | Year: 2013

Using a stand-alone pentaplex comprising two standard-length short tandem repeats (STRs): D12S391 and D1S1656 plus three mini-STRs: D2S441, D10S1248 and D22S1045, all recently adopted to extend the European Standard Set (ESS) STRs, we have examined the genotyping performance of the new markers in 111 challenging casework samples. Although commercial kits now combine the five new STRs with existing core loci, we found the ESS-pentaplex we developed in-house performed better than both MiniFiler (comprising eight miniaturized STRs) and the NGM kit that includes the new STRs in a 15-marker multiplex. Our findings suggest at least part of the improved sensitivity of recently available ESS STRs can be attributed to the loci themselves as well as applying long-standing, robust primer designs that were first designed for the extended ESS markers by the laboratories that originally developed them. Therefore the ESS-pentaplex provides an ideal adjunct to Identifiler or MiniFiler to allow laboratories to assess the new STRs alongside existing standard loci, measure performance with challenging material and generate population frequency data ahead of a final decision on which additional STRs will extend the reconfigured CODIS core set. © 2012 Elsevier Ireland Ltd.

Presciuttini S.,University of Pisa | Toni C.,University of Pisa | Alu M.,University of Modena and Reggio Emilia | Asmundo A.,Messina University | And 15 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

We collected published and unpublished data from 17 contributing groups participating in the GeFI (Italian Forensic Geneticists). The total number of typed subjects was 1114 males and 777 females, coming from 11 regions of North, Centre, and South Italy, and Sardinia. Individual's multilocus genotypes included 4-12 loci. The total number of typed markers was 29, scattered along the X-chromosome genetic map in several clusters; the most used marker was DXS7423 (2429 gene copies); the mean number of subjects typed per marker was 336 for males and 208 for females. Data are available online. © 2011 Elsevier Ireland Ltd.

Barbaro A.,SIMEF | Cormaci P.,SIMEF | Votano S.,SIMEF
Forensic Science International: Genetics Supplement Series | Year: 2011

To fully maximize the utility of DNA databases for tracing the origins of criminal activity, the streamlining of database sample processing must also be accompanied by high performance chemistries. Blood and buccal samples are often used for convicted-felon DNA profile databases, as these samples are easy to obtain. The AmpF. lSTR NGM ™ (Next Generation Multiplex) kit provides enhanced performance on degraded and inhibited samples and tolerance to PCR inhibitors, so we tested the kit ability to amplify directly blood and buccal samples deposited on special collecting cards. The ability to amplify stored DNA directly would allow the lab to get results more efficiently by eliminating the DNA purification step, minimizing the possibility of contamination reducing costs and time accelerating testing by as much as 30% so that lab faster would send consistent results to national database. © 2011 Elsevier Ireland Ltd.

Barbaro A.,SIMEF | Cormaci P.,SIMEF | La Marca A.,SIMEF
Forensic Science International: Genetics Supplement Series | Year: 2011

A genetic population study for SE33 locus has performed from a sample of 130 individuals coming from South of Italy (Calabria) since at least 3 generations, using the next generation multiplex AmpFlSTR NGM SElect™ PCR Amplification Kit by Applied Biosystems. Allele frequencies and statistical parameters of forensic interest (Power of Discrimination, Power of Exclusion Matching Probability, etc.) were calculated using PowerStats v1.2 software. Results demonstrate the usefulness of SE33 for forensic identification, which should be added to the set of STR loci routinely studied in caseworks and in complex paternity cases. © 2011 Elsevier Ireland Ltd.

Barbaro A.,SIMEF | Cormaci P.,SIMEF | Falcone G.,SIMEF
Forensic Science International: Genetics Supplement Series | Year: 2011

Isolation of DNA from forensic samples is strongly conditioned by many factors such as wide variety of samples, degradation by environmental exposure, presence of PCR inhibitors or limited quantity of starting material. BTA performance in the extraction of genomic DNA has been evaluated from real casework powdered bones, teethes, carbonized tissues cigarettes, and tape adhesive lifts. DNA extracted from these samples was in sufficient quantities for downstream applications; the method was successfully able to remove inhibitors producing highly purified DNA; IPC values of Quantifiler® Human DNA Quantification Kit were consistent and within the normal range. The combination of BTA+AmpFℓSTR NGM PCR Amplification Kit provided a powerful tool for the typing of difficult forensic samples. © 2011 Elsevier Ireland Ltd.

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