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Nakhon Pathom, Thailand

Dee-In W.,Silpakom University Sanam Chant Palace | Thepsithar C.,Silpakom University Sanam Chant Palace | Thongpukdee A.,Silpakom University Sanam Chant Palace | Chanjirakul K.,Silpakom University Sanam Chant Palace | Obsuwan K.,Silpakom University Sanam Chant Palace
Acta Horticulturae | Year: 2014

Shoots, about 1 cm long, of chrysanthemum (Dendranthema grandiflora) 'Cayman' were cultured on modified MS medium supplemented with 0.5-1.5 mg L -1 paclobutrazol (PBZ) singly or in combinations with 1-2 mg L -1 N6-benzyladenine (BA). All explants were cultured for two-culture cycles with a 5-week interval. For treatments comprising of the combinations of PBZ and BA; BA was omitted in the second culture cycle. Length of shoots (1.02-1.45 cm) and number of nodes (4.1-7.9) were reduced when higher concentrations of PBZ or BA were applied, compared to the control set without PBZ and BA (9.82 cm long, 10.6 nodes and 1.0 shoot). Higher numbers of shoots (11.3-15.7) were found from BA while only one shoot was obtained from PBZ. Medium containing 1.5 mg L-1 PBZ in combinations with 1.0 or 2.0 mg L-1 BA provided the best results with 0.50-0.78 cm long, 7.6-8.0 nodes and 4.0-4.5 shoots at the first culture cycle and 4.8-5.52 cm long, 7.4-7.6 nodes and 2.1-2.8 shoots at the second culture cycle. Source


Thongpukdee A.,Silpakom University Sanam Chant Palace | Chanjirakul K.,Silpakom University Sanam Chant Palace | Thepsithar C.,Silpakom University Sanam Chant Palace | Obsuwan K.,Silpakom University Sanam Chant Palace | Chantadech R.,Silpakom University Sanam Chant Palace
Acta Horticulturae | Year: 2014

In vitro salt tolerance and the efficiency of L-proline as salt protectant or induced salt tolerance of chrysanthemum (Dendranthema glandiflora) were investigated. Shoots, about 1 cm long, of 'Money Maker Improve' chrysanthemum were cultured on modified MS media supplemented with 0-200 mM NaCl for 2 culture cycles with a four-week interval. Shoot length, number of shoots and total fresh weight were gradually decreased when higher concentrations of NaCl were added to the media. At the concentrations of 110, 140 and 170 mM NaCl, shoot length (2.14-1.57 cm long), leaf number (14.2-9.4) and total fresh weight (0.99-0.61 g) were less than those grown at the concentration of 0 and 20 mM NaCl (8.17 and 7.56 cm long, 22.8 and 19.5 leaves, 2.02 and 1.40 g total fresh weight, respectively). L-proline at the concentrations of 1 or 10 mM was added into the medium containing 110-170 mM NaCl to induce salt tolerance of the shoots. It was found that 1 mM L-proline induced higher salt tolerance of shoots (providing 6.10-2.52 cm height, 22.9-11.6 leaves and 3.02-1.03 g fresh weight, repectively) than 10 mM L-proline did (2.72-1.18 cm height, 15.8-14.3 leaves and 1.99-0.97 g fresh weight, respectively). Source

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