Sikkim State Council of Science and Technology

Gangtok, India

Sikkim State Council of Science and Technology

Gangtok, India
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Goyal A.K.,North Bengal University | Goyal A.K.,Assam University | Pradhan S.,Sikkim State Council of Science and Technology | Basistha B.C.,Sikkim State Council of Science and Technology | Sen A.,North Bengal University
3 Biotech | Year: 2015

Dendrocalamus strictus popularly known as ‘Male bamboo’ is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants. © 2014, The Author(s).


Basistha B.C.,Sikkim State Council of Science and Technology | Sharma N.P.,Sikkim State Council of Science and Technology | Lepcha L.,Sikkim State Council of Science and Technology | Arrawatia M.L.,Sikkim State Council of Science and Technology | Sen A.,North Bengal University
Symbiosis | Year: 2010

Hippophae salicifolia D. Don or Sea buckthorn is one species that has great potential for improving ecological and economic developments in the mountain areas of North Sikkim. The plant is found naturally at altitudes ranging from 2,377 to 3,093 m. in riverine (river side or torrential side) and non-riverine (non river or non torrential side) areas where geo-physico-chemical parameters like aerial temperature, aerial moisture, soil pH, soil temperature, and soil moisture varied significantly. We analyzed ecological parameters and species association using standard quadrates. Actinorhizal plants invade nitrogen-poor soils because of their ability to form root nodule symbioses with N 2-fixing actinomycetes known as Frankia. Variation in nodulation with respect to plant height was also observed. This was higher in riverine areas compared to non riverine sites at higher altitudes. © Springer Science+Business Media B.V. 2009.


Middha S.K.,Maharani Lakshmi Ammanni College for Women | Goyal A.K.,North Bengal University | Faizan S.A.,M.S. Ramaiah Institute of Technology | Sanghamitra N.,M.S. Ramaiah Institute of Technology | And 2 more authors.
Journal of Biosciences | Year: 2013

Type 2 diabetes is an inevitably progressive disease, with irreversible β cell failure. Glycogen synthase kinase and Glukokinase, two important enzymes with diverse biological actions in carbohydrate metabolism, are promising targets for developing novel antidiabetic drugs. A combinatorial structure-based molecular docking and pharmacophore modelling study was performed with the compounds of Hippophae salicifolia and H. rhamnoides as inhibitors. Docking with Discovery Studio 3.5 revealed that two compounds from H. Salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, viz Isorhamnetin-3-rhamnoside and Isorhamnetin-7-glucoside, bind significantly to the GSK-3 β receptor and play a role in its inhibition; whereas in the case of Glucokinase, only one compound from both the plants, i.e. vitamin C, had good binding characteristics capable of activation. The results help to understand the type of interactions that occur between the ligands and the receptors. Toxicity predictions revealed that none of the compounds had hepatotoxic effects and had good absorption as well as solubility characteristics. The compounds did not possess plasma protein-binding, crossing blood-brain barrier ability. Further, in vivo and in vitro studies need to be performed to prove that these compounds can be used effectively as antidiabetic drugs. © 2013 Indian Academy of Sciences.

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