Shri Siddhartha Medical College

Tumkūr, India

Shri Siddhartha Medical College

Tumkūr, India
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Anusuya Devi D.,Shri Siddhartha Medical College | Ramachander R.,ESIC Medical College
Indian Journal of Public Health Research and Development | Year: 2016

Background: Extended-spectrum beta-lactamases (ESBL) may not always be detected in routine susceptibility tests. This study reports the performance of the cefepime-clavulanate compared with cefotaxime–clavulanate disc diffusion method for the detection of extended-spectrum β-lactamases in AmpC coproducing Enterobacteriaceae. Materials & Methods: Consecutive non-duplicate isolates of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolated from clinical samples were tested for ESBL by both the standard CLSI double-disk diffusion method using ceftazidime and cefotaxime disks and compared with cefepime/cefepime-clavulanate. Isolates were also tested for the presence of AmpC beta-lactamase by AmpC disk test and MBL was detected by EDTA disc potentiation test. Results: Among the 100 clinical isolates tested, ESBL production was seen in 34 (34%), Amp C production in 36 (36%), ESBL in Amp C coproduction in 24 (24%) of the isolates, MBL production in 8 (8%) isolates. Conclusion: The study emphasizes the high prevalence of multidrug resistant enterobacteriacae producing beta-lactamase enzymes of diverse mechanisms. Thus proper antibiotic policy and measures to restrict the indiscriminative use of cephalosporins and carbapenems should be taken to minimize the emergence of this multiple beta-lactamase producing pathogens. © 2016, Indian Journal of Public Health Research and Development. All rights reserved.


Anusuya Devi D.,Shri Siddhartha Medical College | Ramachander R.,Shri Siddhartha Medical College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

Enterobacteriacae producing ESBL, Amp C & Metallo beta lactamases have been increasingly reported worldwide. These organisms usually exhibit multidrug resistance that is not always detected in routine susceptibility tests. This leads to uncontrolled spread of ESBL & Amp C producing organisms and related treatment failures. Hence, detection of ESBL, Amp C & MBL is important in the routine clinical laboratory. A total of 100 consecutive Enterobacteriacae i.e. E.coli, Klebsiella spp, Citrobacter spp, Proteus spp isolates from various clinical samples were included in this study. Detection of ESBL production was done by phenotypic confirmatory test as per CLSI guidelines. Amp C production was detected by Amp C disk test as described by Black et al.MBL was detected by EDTA disc potentiation test. Among the 100 clinical isolates tested, ESBL production was seen in 34 (34%), Amp C production in 36 (36%), ESBL & Amp C coproduction in 24 (24%) of the isolates, MBL production in 8 (8%) isolates. The study emphasizes the high prevalence of multidrug resistant enterobacteriacae producing beta-lactamase enzymes of diverse mechanisms. Thus proper antibiotic policy and measures to restrict the indiscriminative use of cephalosporins and carbapenems should be taken to minimize the emergence of this multiple beta-lactamase producing pathogens.

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