Siddhartha Institute of Pharmaceutical science

Narasaraopet, India

Siddhartha Institute of Pharmaceutical science

Narasaraopet, India
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Mallikarjuna Rao N.,Sri Ramakrishna Institute of Paramedical science | Kumar K.R.,Siddhartha Institute of Pharmaceutical science | Bagyalakshmi J.,Sri Ramakrishna Institute of Paramedical science | Ravi T.K.,Sri Ramakrishna Institute of Paramedical science
International Journal of Research in Pharmaceutical Sciences | Year: 2010

A simple, sensitive, precise and specific reverse phase high performance liquid chromatographic method was developed and validated for the determination of voglibose in bulk and tablet dosage forms. It was found that the excipient in the tablet dosage forms does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on RP-18e, Hibar RT column (250×4.6mm) with a mobile phase composed of 0.025M potassium dihydrogen phosphate pH 2.5: acetonitrile: methanol (40:55:5) in isocratic mode at a flow rate of 1ml/min. As voglibose cannot be directly detected with high sensitivity, it was derivatized with Taurine and sodium periodate. The detection was monitored at 282nm. The calibration curve for voglibose was linear from 100 to 500ng/ml. The interday and intraday precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of voglibose in bulk and its tablet dosage forms. LOD and LOQ for voglibose were found to be 30ng/ml and 100ng/ml. Accuracy (recoveries: 99.8-101.2%) and reproducibility were found to satisfactory. © Pharmascope Foundation.


Konidala S.K.,Siddhartha Institute of Pharmaceutical science
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

A simple validated RP HPLC method for the estimation of Ritonavir in pharmaceutical dosage form and bulk was developed for routine analysis. This method was developed by selecting Agilent TC C18 (250 × 4.6 mm, 5 μ) column as stationary phase and Water: Acetonitrile (20:80 v/v) pH adjusted to 3 as mobile phase. Flow rate of mobile phase was maintained at 1 ml/min at ambient temperature throughout the experiment. Quantification was achieved with ultraviolet (DAD) detection at 239 nm. The retention time obtained for Ritonavir was at 4.3 min. The detector response was linear in the concentration range of 10 - 80μg/ml. This method has been validated and shown to be Specific, Sensitive, Precise, Linear, Accurate, Rugged and Robust. Hence, this method can be applied for routine quality control of Ritonavir in dosage forms as well as in bulk drug.


Mogili R.,Jawaharlal Nehru Technological University Anantapur | Mogili R.,Siddhartha Institute of Pharmaceutical science | Kanala K.,Jawaharlal Nehru Technological University Anantapur | Bannoth C.K.,Jawaharlal Nehru Technological University Anantapur
Scientia Pharmaceutica | Year: 2011

In this study, authors developed a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantification of Amisulpride in human plasma using Amisulpride-d5 as an internal standard (IS). Chromatographic separation was performed on Zorbax Bonus-RP C18, 4.6 × 75 mm, 3.5 μm column with an isocratic mobile phase composed of 0.2% formic acid:methanol (35:65 v/v), at a flow-rate of 0.5 mL/min. Amisulpride, Amisulpride-d5 was detected at m/z 370.1→242.1 and 375.1→242.1. The drug and the IS were extracted by a liquid-liquid extraction method. The method was validated over a linear concentration range of 2.0-2500.0 ng/mL for Amisulpride with a correlation coefficient of (r2) ≥ 0.9982. This method demonstrated intra- and inter-day precision within 0.9 to 1.7 and 1.5 to 2.8 % and intra- and inter-day accuracy within 98.3 to 101.5 and 96.0 to 101.0 % for Amisulpride. Amisulpride was found to be stable at 3 freeze-thaw cycles, bench top and auto sampler stability studies. The developed method was successfully applied to a pharmacokinetic study. © Mogili et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria.


Mogili R.,Jawaharlal Nehru Technological University Anantapur | Mogili R.,Siddhartha Institute of Pharmaceutical science | Kanala K.,Jawaharlal Nehru Technological University Anantapur | Bannoth C.K.,Jawaharlal Nehru Technological University Anantapur
Der Pharmacia Lettre | Year: 2011

The present study aims at developing a simple, sensitive and specific liquid chromatography.tandem mass spectrometry (LC-MS/MS) method for the quantification of Esomeprazole (EO) in human plasma using Omeprazole-d3 (OMD3) as an internal standard (IS). Chromatographic separation was performed on Xbridge C18, 50 x 4.6 mm, 5 μm column with an isocratic mobile phase composed of 5mM Ammonium formate (pH 9.0) : Acetonitrile (70:30 v/v) at a flow-rate of 0.6 mL/min. EO and OMD3 were detected with proton adducts at m/z 346.1→198.0 and 349.0→197.9 in multiple reaction monitoring (MRM) positive mode respectively. EO and OMD3 were extracted by Precipitation method. The method was validated over a linear concentration range of 5.0-2000.0 ng/mL with a correlation coefficient of (r2) ≥ 0.9989. This method demonstrated intra and inter-day Precision within 1.6 to 2.3 and 2.0 to 2.2 % and Accuracy within 97.9 to 100.7 and 98.0 to 99.3 %. EO was found to be stable throughout freeze-thawing cycles, bench top and postoperative stability studies. This method was utilized successfully for the analysis of plasma samples following oral administration of EO (40 mg) in 27 healthy Indian male human volunteers under fasting conditions.


Ravi Kumar K.,Siddhartha Institute of Pharmaceutical science | Babu Rao Ch.,Siddhartha Institute of Pharmaceutical science | Chandra Sekhar K.B.,Jawaharlal Nehru Technological University Anantapur
International Journal of ChemTech Research | Year: 2010

A simple, rapid and selective RP-HPLC method has been developed for quantification Capecitabine from bulk drug and pharmaceutical formulations using a mobile phase consisting mixture of Buffer and acetonitrile in the ratio (80:40) (v/v) at the flow rate of 1.2 ml/min. An Hypersil BDS C8, 250, 5mm, was used as stationary phase. The retention time for Capecitabine was 15min. Linearity was observed in the concentration range of 40 to 60 μg/ml, with good linearity response greater than 0.997. The mean % recovery obtained is 100.1%. The proposed method is precise, accurate, selective and rapid for the determination of Capecitabine in capsules.


Srinivasan R.,Siddhartha institute of pharmaceutical science | Manoj L.,Siddhartha institute of pharmaceutical science | Kalyani M.,Siddhartha institute of pharmaceutical science | Jyothi K.,Siddhartha institute of pharmaceutical science | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2011

The brain and spinal column make up the central nervous system (CNS), where all vital functions of the body are controlled. When tumors arise in the central nervous system, they are especially problematic because a persons thought processes and movements can be affected. These tumors can also be difficult to treat because the tissues surrounding a tumor that may be affected by surgery or radiation may play a vital role in functioning. There are two broad types of cancers occurring within this system. Primary tumors originate in the central nervous system, whereas secondary tumors migrate from cancers located elsewhere in the body, such as breast cancers. Secondary, or metastatic, brain tumors, are more common than primary brain cancers. This section focuses on primary stages on brain cancer.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Ravali B.,Siddhartha Institute of Pharmaceutical science | Suvarchala P.,Siddhartha Institute of Pharmaceutical science | Honey A.,Siddhartha Institute of Pharmaceutical science | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2011

Leucas aspera commonly known as 'Thummichittu' is distributed throughout India and in the plains of Mauritius, java and philippines. The plant is used traditionally as an antipyretic and insecticide. Medicinally, it has been proven to possess various pharmacological activities like antifungal, antioxidant, antimicrobial, antinociceptive and cytotoxic activity and as an antidote to snake venom .Leucas leaves are used in chronic rheumatism. Further studies reveal the presence of various phytochemical constituents mainly triterpenoids, oleanolic acid, ursolic acid and b-sitosterol, nicotine, sterols, glucoside, diterpenes, phenolic compounds (4-(24-hydroxy-1-oxo-5-n-propyltetracosanyl)-phenol). These studies reveal that L. aspera is a source of medicinally active compounds and have various pharmacological effects; hence, this drug encourage finding its new therapeutic uses.


Macherla S.,Siddhartha Institute of Pharmaceutical science | Murahari N.K.,Siddhartha Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2012

Stem cells are biological cells found in all multicellular organisms, that can divide through mitosis and differentiate into diverse specialized cell types and can self renew to produce more stem cells. In mammals, there are two broad types of stem cells: embryonic stem cells that are isolated from the inner cell mass of blastocysts, and adult stem cells that are found in various tissues. In adult organisms, stem cells and progenitor cells act as a repair system for the body, replenishing adult tissues. In a developing embryo, stem cells can differentiate into all the specialized cells, but also maintain the normal turnover of regenerative organs, such as blood, skin, or intestinal tissues. Medical researchers believe that stem cell therapy has the potential to dramatically change the treatment of human disease. A number of adult stem cell therapies already exist, particularly bone marrow transplants that are used to treat leukemia. In the future, medical researchers anticipate being able to use technologies derived from stem cell research to treat a wide variety of diseases including cancer, Parkinson's disease, spinal cord injuries, Amyotrophic lateral sclerosis, multiple sclerosis, and muscle damage, amongst a number of other impairments and conditions.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Lakshmana G.,Siddhartha Institute of Pharmaceutical science | Anjinayalu B.,Siddhartha Institute of Pharmaceutical science | Anil Kumar D.,Siddhartha Institute of Pharmaceutical science | Shalem Raju K.,Siddhartha Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2014

Naturevolution is a term necessary to evaluate ourselves in term metabolism of natural metabolites. Our studies insist on natural products more extremely powerful treatment for cancer cures. The truth is they actually hate people using any form of natural cures for cancer. Mainly anticancer agents are obtained from plants (vincristine, vinblastine, etoposide, paclitaxel, camptothecin, topotecan and irinotecan), marine organisms (citarabine, aplidine and dolastatin 10) and microorganisms (dactinomycin, bleomycin and doxorubicin)some of the fruits and vegetables also has anticancer activity.some of the plant products which are in daily usage are capsaicin (red chilli), diosgenin (fenugreek), 6-gingerol (ginger), ellagic acid (pomegranate), curcumin (turmeric), resveratrol (red grapes, peanuts and berries), genistein (soybean), diallyl sulfide (allium), S-allyl cysteine (allium), allicin (garlic), lycopene (tomato), ursolic acid (apple, pears, prunes), silymarin (milk thistle), anethol (anise, camphor, and fennel), catechins (green tea), eugenol (cloves), indole-3- carbinol (cruciferous vegetables), limonene (citrus fruits), beta carotene (carrots), and dietary fiber.in this review the active principle obtained from natural products and novel lead compounds along with mechanism of action has been included.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Ravi Kumar K.,Siddhartha Institute of Pharmaceutical science | Prem Kumar P.,Siddhartha Institute of Pharmaceutical science
International Journal of ChemTech Research | Year: 2010

The aim of the study was to design, synthesize and investigate the antimicrobial & fungal activity of some α N-Phthilimido derivatives of amino acids. The chemical structures of the titled compound were confirmed by IR, 13CNMR and elemental analysis. All the compounds are screened for antimicrobial activity against gram positive, gram negative bacteria (Escherichia coli, Klebsiella, Staphylococcus epidermitis, Bacillus cereus, Micrococcus leteus, Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger).

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