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Mogili R.,Jawaharlal Nehru Technological University Anantapur | Mogili R.,Siddhartha Institute of Pharmaceutical science | Kanala K.,Jawaharlal Nehru Technological University Anantapur | Chandu B.R.,P.A. College | Bannoth C.K.,Jawaharlal Nehru Technological University Anantapur
Chromatographia | Year: 2011

A simple, sensitive, selective, rapid, rugged, reproducible and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for quantitative estimation of rizatriptan (RZ) in human plasma using rizatriptan-d 6 (RZD6) as internal standard (IS). Chromatographic separation was performed on Ascentis Express RP Amide C18, 50 × 4.6 mm, 2.7 μm column with isocratic mobile phase composed of 10 mM ammonium formate:acetonitrile (20:80 v/v) at flow rate of 0.5 mL min-1. RZ and RZD6 were detected with proton adducts at m/z (amu) 270.2 → 201.2 and 276.1 → 207.1, respectively, in multiple reaction monitoring (MRM) positive mode. Liquid-liquid extraction was used and validated over a linear concentration range of 0.1-100.0 ng mL-1 with correlation coefficient r 2 ≥ 0.9981. The limit of quantification (LOQ) and limit of detection (LOD) were found to be 0.1 ng mL-1 and 12.5 fg, respectively. Intra- and inter-day precision were within 1.7-3.1% and 2.8-3.7%, and accuracy within 96.0-101.7% and 99.7-101.4% for RZ. Drug was found to be stable throughout three freeze-thaw cycles. The method was successfully employed for analysis of plasma samples following oral administration of RZ (10 mg) in 25 healthy Indian male human volunteers under fasting conditions. © 2011 Springer-Verlag.


Ravi Kumar K.,Siddhartha Institute of Pharmaceutical science | Babu Rao Ch.,Siddhartha Institute of Pharmaceutical science | Chandra Sekhar K.B.,Jawaharlal Nehru Technological University Anantapur
International Journal of ChemTech Research | Year: 2010

A simple, rapid and selective RP-HPLC method has been developed for quantification Capecitabine from bulk drug and pharmaceutical formulations using a mobile phase consisting mixture of Buffer and acetonitrile in the ratio (80:40) (v/v) at the flow rate of 1.2 ml/min. An Hypersil BDS C8, 250, 5mm, was used as stationary phase. The retention time for Capecitabine was 15min. Linearity was observed in the concentration range of 40 to 60 μg/ml, with good linearity response greater than 0.997. The mean % recovery obtained is 100.1%. The proposed method is precise, accurate, selective and rapid for the determination of Capecitabine in capsules.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Jayakar B.,Salem College
International Journal of Pharma and Bio Sciences | Year: 2011

The present study deals with the application of α N-Phthilimido and acetylated tyrosine and determination of tyrosinase inhibitory activity which serves as an useful target in the treatment of hyper pigmentation skin disorder. The tyrosinase inhibitory activity of α N-Phthilimido tyrosine found to be highly significant (p< 0.0001) when compared with that of acetyltyrosine.


Jayakar B.,Salem College | Srinivasan R.,Siddhartha Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2010

The phthaloyl and acetyl group are common protecting groups from are common protecting group for amines in organic synthesis 1 and are also important pharmacophores 2. Common method are imide synthesis include dehydrative condensation of an anhydride and amino acid at high temperature, the acid-catalyzed cyclization of N-substituted amic acid. Abdol Reza Hajipour 3, rapidly synthesised Phthalimide derivatives by microwave irradiation. A simple extremely fast, and high yielding method for the reaction of phthalic anhydride with a number of amino acids using microwave irradiation under solventless dry condition has been developed. The aim of the study was to design, synthesize and investigate the antimicrobial and antifungal activities, anticancer activities of some α N-Phthilimido and acetylated derivatives of amino acids. The chemical structures of the titled compound were confirmed by IR, 13CNMR and elemental analysis. All the compounds were screened for antimicrobial activity against gram positive, gram negative bacteria (Escherichia coli, Klebsiella, Staphylococcus epidermitis, Bacillus cereus, Micrococcus leteus, Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger).


Srinivasan R.,Siddhartha institute of pharmaceutical science | Manoj L.,Siddhartha institute of pharmaceutical science | Kalyani M.,Siddhartha institute of pharmaceutical science | Jyothi K.,Siddhartha institute of pharmaceutical science | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2011

The brain and spinal column make up the central nervous system (CNS), where all vital functions of the body are controlled. When tumors arise in the central nervous system, they are especially problematic because a persons thought processes and movements can be affected. These tumors can also be difficult to treat because the tissues surrounding a tumor that may be affected by surgery or radiation may play a vital role in functioning. There are two broad types of cancers occurring within this system. Primary tumors originate in the central nervous system, whereas secondary tumors migrate from cancers located elsewhere in the body, such as breast cancers. Secondary, or metastatic, brain tumors, are more common than primary brain cancers. This section focuses on primary stages on brain cancer.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Ravali B.,Siddhartha Institute of Pharmaceutical science | Suvarchala P.,Siddhartha Institute of Pharmaceutical science | Honey A.,Siddhartha Institute of Pharmaceutical science | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2011

Leucas aspera commonly known as 'Thummichittu' is distributed throughout India and in the plains of Mauritius, java and philippines. The plant is used traditionally as an antipyretic and insecticide. Medicinally, it has been proven to possess various pharmacological activities like antifungal, antioxidant, antimicrobial, antinociceptive and cytotoxic activity and as an antidote to snake venom .Leucas leaves are used in chronic rheumatism. Further studies reveal the presence of various phytochemical constituents mainly triterpenoids, oleanolic acid, ursolic acid and b-sitosterol, nicotine, sterols, glucoside, diterpenes, phenolic compounds (4-(24-hydroxy-1-oxo-5-n-propyltetracosanyl)-phenol). These studies reveal that L. aspera is a source of medicinally active compounds and have various pharmacological effects; hence, this drug encourage finding its new therapeutic uses.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Reddy C.U.M.,Sri Ramachandra ollege of Pharmacy | Jayakar B.,Salem College
International Journal of Pharma and Bio Sciences | Year: 2011

After glucose, amino acids like glutamine, methonine, glycine, alanine, phenyl alanine are a major metabolite necessary synthesizing for the cancer cell. In the synthesis of DNA and RNA, major portions of nitrogen atoms are supplied by amino acids. Structural variants of amino acids may antagonize enzymes involved in DNA and RNA synthesis. A QSAR (quantitative structure-activity relationships) study was performed on some previously synthesized amino acid analogues in order to get insight in the substitution requirements for their anticancer activity as well as to overcome the symmetry restriction of De Novo model and time consuming determination of partition coefficients of Hansch analysis. A good QSAR model was obtained considering anticancer activity, i.e., log % of tumor weight inhibition which expresses the biological activity, of three phthilimido derivatives which is a analogues of amino acid as dependent variable and substitution contribution at specific position as independent variable as evidenced by the statistical data (r = 0.8122, s = 0.1196, F = 1.3755).


Macherla S.,Siddhartha Institute of Pharmaceutical science | Murahari N.K.,Siddhartha Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2012

Stem cells are biological cells found in all multicellular organisms, that can divide through mitosis and differentiate into diverse specialized cell types and can self renew to produce more stem cells. In mammals, there are two broad types of stem cells: embryonic stem cells that are isolated from the inner cell mass of blastocysts, and adult stem cells that are found in various tissues. In adult organisms, stem cells and progenitor cells act as a repair system for the body, replenishing adult tissues. In a developing embryo, stem cells can differentiate into all the specialized cells, but also maintain the normal turnover of regenerative organs, such as blood, skin, or intestinal tissues. Medical researchers believe that stem cell therapy has the potential to dramatically change the treatment of human disease. A number of adult stem cell therapies already exist, particularly bone marrow transplants that are used to treat leukemia. In the future, medical researchers anticipate being able to use technologies derived from stem cell research to treat a wide variety of diseases including cancer, Parkinson's disease, spinal cord injuries, Amyotrophic lateral sclerosis, multiple sclerosis, and muscle damage, amongst a number of other impairments and conditions.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Lakshmana G.,Siddhartha Institute of Pharmaceutical science | Anjinayalu B.,Siddhartha Institute of Pharmaceutical science | Anil Kumar D.,Siddhartha Institute of Pharmaceutical science | Shalem Raju K.,Siddhartha Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2014

Naturevolution is a term necessary to evaluate ourselves in term metabolism of natural metabolites. Our studies insist on natural products more extremely powerful treatment for cancer cures. The truth is they actually hate people using any form of natural cures for cancer. Mainly anticancer agents are obtained from plants (vincristine, vinblastine, etoposide, paclitaxel, camptothecin, topotecan and irinotecan), marine organisms (citarabine, aplidine and dolastatin 10) and microorganisms (dactinomycin, bleomycin and doxorubicin)some of the fruits and vegetables also has anticancer activity.some of the plant products which are in daily usage are capsaicin (red chilli), diosgenin (fenugreek), 6-gingerol (ginger), ellagic acid (pomegranate), curcumin (turmeric), resveratrol (red grapes, peanuts and berries), genistein (soybean), diallyl sulfide (allium), S-allyl cysteine (allium), allicin (garlic), lycopene (tomato), ursolic acid (apple, pears, prunes), silymarin (milk thistle), anethol (anise, camphor, and fennel), catechins (green tea), eugenol (cloves), indole-3- carbinol (cruciferous vegetables), limonene (citrus fruits), beta carotene (carrots), and dietary fiber.in this review the active principle obtained from natural products and novel lead compounds along with mechanism of action has been included.


Srinivasan R.,Siddhartha Institute of Pharmaceutical science | Ravi Kumar K.,Siddhartha Institute of Pharmaceutical science | Prem Kumar P.,Siddhartha Institute of Pharmaceutical science
International Journal of ChemTech Research | Year: 2010

The aim of the study was to design, synthesize and investigate the antimicrobial & fungal activity of some α N-Phthilimido derivatives of amino acids. The chemical structures of the titled compound were confirmed by IR, 13CNMR and elemental analysis. All the compounds are screened for antimicrobial activity against gram positive, gram negative bacteria (Escherichia coli, Klebsiella, Staphylococcus epidermitis, Bacillus cereus, Micrococcus leteus, Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger).

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