Sichuan UniversitySichuan

West, China

Sichuan UniversitySichuan

West, China
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Zhang S.,Sichuan UniversitySichuan | Li G.,Chinese Academy of Sciences | Liu H.,Chinese Academy of Sciences | Wang Y.,Sichuan UniversitySichuan | And 3 more authors.
RSC Advances | Year: 2017

The mimics of vitamin B6-dependent enzymes that catalyzed an enantioselective full transamination in the pure aqueous phase have been realized for the first time through the establishment of a new “pyridoxal 5′-phosphate (PLP) catalyzed non-covalent cyclodextrin (CD)-keto acid inclusion complexes” system, and various optically active amino acids have been obtained. © The Royal Society of Chemistry.

He L.,University of Sichuan | Huang L.,University of Sichuan | Zhang S.,University of Sichuan | Chen Y.,University of Sichuan | And 2 more authors.
Materials Science and Engineering C | Year: 2017

Bio-inspired phosphorylcholine modification on material surface has shown great promise in constructing biocompatible materials. In this study, poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was grown on poly(ε-caprolactone) (PCL) surface in order to suppress protein adsorption and cells adhesion and to improve blood compatibility. The initiator for surface initiated atom transfer radical polymerization (SI-ATRP) was covalently tethered on PCL surface and then PMPC brushes with diverse graft amounts were grafted to PCL film. X-ray photoelectron spectroscopy (XPS) and water contact angle measurement were used to characterize the modified sheets. The PMPC-grafted PCL sheets showed lower protein adsorption, maintained secondary structure of detached protein, and suppressed adhesion and pseudopodium formation of the platelets, along with keeping longer activated partial thromboplastin time (APTT) in comparison with PCL membranes. At the same time, PMPC-grafted PCL sheets suppressed the LO2 cells adhesion. These results showed that phosphorylcholine SI-ATRP modification on PCL surface may provide PCL more biocompatible in biomaterial applications. © 2017

Zhang Y.-T.,Hong Kong Polytechnic University | Zhang Y.-T.,Sichuan UniversitySichuan | Li-Tsang C.W.P.,Hong Kong Polytechnic University | Au R.K.C.,Hong Kong Polytechnic University
Hong Kong Journal of Occupational Therapy | Year: 2017

Objective/Background To review the effect of mechanical stretch on hypertrophic scars after burn injuries. Methods A systematic review of all controlled trials related to the effect of mechanical stretch on post burn hypertrophic scars was conducted. Studies of conservative scar managements that applied mechanical forces parallel to the scar surface, including stretching exercise, massage, and splinting, were appraised. Eligible studies published in English between 1995 and 2016 were extracted from The Cochrane Library, MEDLINE, CINAHL, Science direct, SPORTDiscus, and Physiotherapy Evidence Database Scale (PEDro). The journals were further screened with inclusion and exclusion criteria. PEDro was selected for further analysis and appraisal. Results There were 853 articles identified. After a standardized screening mechanism stipulated, only nine full-text articles were selected for critical appraisal using PEDro. There were five articles of high quality, two of fair quality, and two of poor quality. Detailed training regime and outcomes of nine studies were summarised, including two studies with stretching exercise, six studies with massage, and one study with splinting. The physical parameters of scar assessments and the range of motion on affected areas were compared. Conclusion From extensive literature search, there was no strong evidence indicating the positive effect of mechanical stretch using stretching exercise, massage, or splinting on hypertrophic scars. A firm conclusion cannot be drawn for the discrepancy of outcome measures and varied effectiveness. Most of the included studies lacked objective evaluation or control group for comparison. Further high quality studies with larger sample size and using standardized measurements are needed. © 2017

Ni Y.-N.,Sichuan UniversitySichuan | Luo J.,Sichuan UniversitySichuan | Yu H.,Sichuan UniversitySichuan | Liu D.,Sichuan UniversitySichuan | And 4 more authors.
Chest | Year: 2017

Background The effects of high-flow nasal cannula (HFNC) on adult patients with acute respiratory failure (ARF) are controversial. We aimed to further determine the effectiveness of HFNC in reducing the rate of endotracheal intubation in adult patients with ARF by comparison to noninvasive positive pressure ventilation (NIPPV) and conventional oxygen therapy (COT). Methods The PubMed, Embase, Medline, and the Cochrane Central Register of Controlled Trials databases, as well as the Information Sciences Institute Web of Science, were searched for all controlled studies that compared HFNC with NIPPV and COT in adult patients with ARF. The primary outcome was the rate of endotracheal intubation; the secondary outcomes were ICU mortality and length of ICU stay. Results Eighteen trials with a total of 3,881 patients were pooled in our final studies. Except for ICU mortality (I2 = 67%, χ2 = 12.21, P = 02) and rate of endotracheal intubation (I2 = 63%, χ2 = 13.51, P = 02) between HFNC and NIPPV, no significant heterogeneity was found in outcome measures. Compared with COT, HFNC was associated with a lower rate of endotracheal intubation (z = 2.55, P = 01) while no significant difference was found in the comparison with NIPPV (z = 1.40, P = 16). As for ICU mortality and length of ICU stay, HFNC did not exhibit any advantage over either COT or NIPPV. Conclusions In patients with ARF, HFNC is a more reliable alternative than NIPPV to reduce the rate of endotracheal intubation than COT. © 2017 American College of Chest Physicians

Xie C.,Sichuan UniversitySichuan | Ma B.,Sichuan UniversitySichuan | Wang N.,Sichuan UniversitySichuan | Wan L.,Sichuan UniversitySichuan
Experimental Biology and Medicine | Year: 2017

Liver fibrosis assessment is essential to make a prognosis and to determine the appropriate anti-fibrosis treatment. Non-invasive serum markers are widely studied in patients to assess liver fibrosis due to the limitations of liver biopsy. When using animal models to study the mechanism and intervention of hepatic fibrosis, serum markers might be useful for the continuous assessment of liver fibrosis in individual animals, which could avoid the influence of biological differences between individuals. However, it is unclear whether serum markers can assess hepatic fibrosis in the animal model. In the present study, we evaluated and compared the ability of four serum markers to assess liver fibrosis in bile duct ligation mice. According to the stages of liver fibrosis assessed by pathological changes, mice in this study were divided into five groups (F0, F1, F2, F3, and F4). Subsequently, four serum markers, aspartate aminotransferase-to-alanine aminotransferase ratio (AAR), aspartate aminotransferase-to-platelet ratio index (APRI), fibrosis index based on the 4 factors (FIB-4), and Forns Index, were calculated for each group. Furthermore, the correlations between serum markers and pathological stages and the ability of serological markers to evaluate liver fibrosis were analyzed. AAR, APRI, FIB-4, and Forns Index could significantly distinguish F0-2 from F3-4 mice. APRI, FIB-4, and Forns Index could detect F0-3 from F4 mice. Among these four markers, FIB-4 was the best able to distinguish ≥F2 and ≥F3, with area under the curve values of 0.882 and 0.92, respectively. Forns Index was best for diagnosing F4 with area under the curve value of 0.879. These results demonstrated that serum markers could be used for assessing liver fibrosis in bile duct ligation mice, and therefore, these markers might lead to more accurate diagnostic and therapeutic studies through continuous monitoring in individual animals. Impact statement: The assessment of liver fibrosis is essential for making a prognosis and determining the appropriate anti-fibrosis treatment. In studies focusing on the mechanism and treatment of liver fibrosis using animal models, it would be more accurate to continuously evaluate liver fibrosis in a single animal to avoid individual biological differences. Unfortunately, it is difficult to perform continuous assessment through liver biopsy in the most commonly used rodent models. It is unclear whether serum markers, which have been used in hepatic fibrosis patients, could be used in animal models. Our results demonstrate that serum markers could be used for assessing liver fibrosis in bile duct ligation mice. This study might contribute to more accurate diagnostic and therapeutic studies through continuous monitoring in individual animals. © 2017, © 2017 by the Society for Experimental Biology and Medicine.

Li W.,Sichuan UniversitySichuan | Peng J.,Sichuan UniversitySichuan | Tan L.,Sichuan UniversitySichuan | Wu J.,Sichuan UniversitySichuan | And 4 more authors.
Biomaterials | Year: 2016

Patients suffering from cancer have benefited from combination therapy. Nanocarriers are the ideal candidates for combination therapy. In this study, we constructed docetaxel (DTX) loaded micellar nanomedicines co-loaded with near infrared (NIR) dye-IR820 for photothermal therapy (PTT)/photodynamic therapy (PDT)/chemotherapy of breast cancer. Lyp-1, a tumor homing peptide, was introduced into the nanosystems to construct the active targeting nanomedicine. In order to deliver IR820 to the tumor site and overcome its short lifetime in vivo, a PEI derivative—PCL-g-PEI was introduced. IR820 with negative charge was formed stable static interaction with the amine groups, meanwhile, the absorption of IR820 in the NIR region was weakened. It indicated that the nanosystem constructed in this study may provide an alternative candidate for mild PTT. By the evaluation of the photothermal conversion in vivo, we can confirm that IR820 has been successfully delivered and effectively accumulated in the tumor site. Furthermore, the tumor cells targeting and anticancer performances of this nanosystem have been studied in vitro and in vivo. The results demonstrated Lyp-1 modification has enhanced the tumor targeting delivery of DTX and IR820. By combining PTT and PDT, DTX nanomedicine efficiently inhibited the growth and metastasis of breast cancer in mice. This nanosystem is a promising candidate for combination therapy of breast cancer. © 2016 Elsevier Ltd

Jiang X.,Sichuan UniversitySichuan | Wang Q.,Sichuan UniversitySichuan | Huang R.,Sichuan UniversitySichuan
Clinical Nuclear Medicine | Year: 2015

Nasal iodine activity can be observed on 123Iodine (123I) or 131I wholebody scintigraphy (WBS) commonly as a normal variant caused by nasal or salivary secretion of the tracer. We encountered 2 patients whose increased accumulation of 131I activity was associated with underlying abnormalities. One patient had a nasal polyp, whereas the other had an abscess. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.

Wang Y.,Sichuan UniversitySichuan | Qiao Y.,Sichuan UniversitySichuan | Li W.,Sichuan UniversitySichuan
International Journal of Clinical and Experimental Medicine | Year: 2016

Objectives: To investigate the predictive value of computed tomography imaging in diagnosis of extratemporal facial nerve schwannoma. Materials and methods: Computed tomography records of eight patients with extratemporal facial nerve schwannoma managed from 2007~2015 were reviewed. Tumors of all cases were totally removed surgically. Intraoperative frozen section examination was employed. Results: computed tomography of one patient whose tumor derived from buccal branch demonstrated imaging of a round mass with clear margin; four out of five patients whose tumors derived from facial nerve trunk revealed a significant tail pointing to the stylomastoid foramen with one exception; one patient whose tumor located at the margin of parotid gland demonstrated round mass just outside the stylomastoid foramen deep to the mastoid process with a tail pointing to the parotid gland; one case of synchronous schwannomas took place in the parotid gland and parapharyngeal space presented obscure tail-like imaging. Diagnoses of schwannoma were inclined in all intraoperative frozen section examinations. There was no tumor relapse in all cases after one to three years follow-up while the tumor-involved facial nerve or nerve branches were disturbed in all cases. Conclusion: Computed tomography findings can provide some useful information for the diagnosis of extratemporal facial nerve schwannoma especially for those derived from nerve trunk. © 2016, E-Century Publishing Corporation. All rights reserved.

Sun L.,West China Hospital of Sichuan UniversitySichuan | Li Y.,Sichuan UniversitySichuan | Yang B.,West China Hospital of Sichuan UniversitySichuan
Biochemical and Biophysical Research Communications | Year: 2016

Long non-coding RNAs (lncRNAs) was found to play critical roles in tumorigenesis, hence, screen of tumor-related lncRNAs, identification of their biological roles is important for understanding the processes of tumorigenesis. In this study, we identified the expressing difference of several tumor-related lncRNAs in breast cancer samples and found that, MEG3, which is downregulated in non-small cell lung cancer (NSCLC) tumor tissues, is also downregulated in breast cancer samples compared with adjacent tissues. For figuring out the effect of MEG3 in breast cancer cells MCF7 and MB231, we overexpressed MEG3 in these cells, and found that it resulted the inhibition of proliferation, colony formation, migration and invasion capacities by enhancing p53’s transcriptional activity on its target genes, including p21, Maspin and KAI1. MEG3 presented similar effects in MB157, which is a p53-null breast cancer cell line, when functional p53 but not p53R273H mutant, which lacks transcriptional activity, was introduced. Surprisingly, overexpression of MEG3 activates p53’s transcriptional activity by decreasing MDM2’s transcription level, and thus stabilizes and accumulates P53. Taken together, our findings indicate that MEG3 is downregulated in breast cancer tissues and affects breast cancer cells’ malignant behaviors, which indicate MEG3 a potential therapeutic target for breast cancer. © 2016

Chen J.,Sichuan Orthopedic HospitalSichuan | Chen J.,Sichuan UniversitySichuan | Xiao J.,Sichuan UniversitySichuan
International Journal of Clinical and Experimental Medicine | Year: 2015

The purpose of our study was to accurately visualize and measure the normal anatomy and size of the pterygoid canal with thin-section (0.5 mm), high-resolution computed tomography (HRCT) as well as multiplanar reconstruction (MPR) and curved planar reconstruction (CPR) technologies to obtain credible and comprehensive information for clinical management. Both axial HRCT and MPR images of the pterygopalatine fossa were obtained in 167 normal adult subjects, who exhibited variable positions of pterygoid canal relative to the sphenoid sinus floor and cavity. The morphology and size of the pterygoid canal was observed and measured, respectively. All pterygoid canals (100%, 334/334) were well delineated on HRCT images. Statistical analyses showed no significant difference between the mean length of the left (12.6 ± 2.3 mm) and right pterygoid canals (12.5 ± 2.9 mm) (P = 0.405). The mean diameter of anterior, median and posterior opening of the left and right pterygoid canals exhibited no significant differences (all P > 0.05); the bilateral median opening possessed the smallest diameter (P < 0.001). Submillimeter, thin-section HRCT scan and appropriate postprocessing reconstruction technologies could clearly visualize the morphologic features of the pterygoid canal and adjacent structures, which may be helpful for making diagnostic and therapeutic decisions. © 2015, E-Century Publishing Corporation. All rights reserved.

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