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Li X.,University of Sichuan | Zeng W.-C.,University of Sichuan | Zhu D.-Y.,University of Sichuan | Feng J.-L.,University of Sichuan | And 4 more authors.
Process Biochemistry | Year: 2017

This study explored the possible application of collagen hydrolysate (CH) in the fermentation process using Bacillus pumilus as probe bacterial strain. When CH acted as an only source of carbon and nitrogen in the culture medium, B. pumilus was observed to show normal characterizations during its growing process. Meanwhile, the utilization degree of CH by B. pumilus could be enhanced when wheat bran was added (used as carbon source) in the fermentation process. It was found that the highest alkaline protease activity was 325 U/mL when only CH was used, while it was increased to 620 U/mL with the addition of wheat bran. Besides, with different combination of soybean meal and CH, it was found that the ability using CH to produce alkaline protease is comparable to that of soybean meal in the presence of wheat bran. Moreover, the growth behaviors of the strain were not affected by inoculation concentrations, but the time of producing alkaline protease was greatly saved as reducing loading volume. Present investigation suggested that CH can be used as carbon and nitrogen sources in the fermentation process. © 2017 Elsevier Ltd


Luo H.,Sichuan Agricultural University | Luo H.,Sichuan UniversityChengdu | Li M.,Sichuan UniversityChengdu | Xu R.,Sichuan UniversityChengdu | And 4 more authors.
Sustainable Environment Research | Year: 2012

The pollution of urban stormwater runoff discharged to river cannot be ignored. Through a mixed stormwater sampling strategy at discharge outlet adjacent to river, this study assessed the urban surface runoff pollution characteristics caused by a variety of land uses in a small urban watershed in the city of Shenzhen in China. The rainfall events were monitored from Oct. 2007 to Sep. 2008. Results show that the pollution caused by the urban surface runoff in studied area is serious and needs to be treated. The first flushes of chemical oxygen demand (COD), suspended solids (SS), biochemical oxygen demand, especially COD and SS, were apparent, but total nitrogen and total phosphorus were not evident. The event mean concentrations (EMCs) of COD were about two times of the value of the nearby cities, Macao and Zhuhai. Comparing with other counties, the EMCs of different pollutants in the studied area in Shenzhen were much higher than the values of South Korea, USA and Canada. In order to treat surface runoff pollution, the values from first 30-40% runoff volume can be considered as split-flow control criteria to enable more effective and economical design of best management practice facilities. © 2012, Chinese Institute of Environmental Engineering. All rights reserved.


PubMed | Capital Medical University, CAS Chengdu Institute of Biology, Sichuan UniversityChengdu and Chengdu University of Technology
Type: | Journal: Frontiers in microbiology | Year: 2016

Dental caries is a biofilm-dependent disease that largely relies on the ability of Streptococcus mutans to synthesize exopolysaccharides. Although the rnc gene is suggested to be involved in virulence mechanisms in many other bacteria, the information regarding it in S. mutans is very limited. Here, using deletion or overexpression mutant assay, we demonstrated that rnc in S. mutans significantly positively regulated exopolysaccharide synthesis and further altered biofilm formation. Meanwhile, the cariogenecity of S. mutans was decreased by deletion of rnc in a specific pathogen-free (SPF) rat model. Interestingly, analyzing the expression at mRNA level, we found the downstream vic locus was repressed by rnc in S. mutans. Using deep sequencing and bioinformatics analysis, for the first time, three putative microRNA-size small RNAs (msRNAs) targeting vicRKX were predicted in S. mutans. The expression levels of these msRNAs were negatively correlated with vicRKX but positively correlated with rnc, indicating rnc probably repressed vicRKX expression through msRNAs at the post-transcriptional level. In all, the results present that rnc has a potential role in the regulation of exopolysaccharide synthesis and can affect vicRKX expressions via post-transcriptional repression in S. mutans. This study provides an alternative avenue for further research aimed at preventing caries.


Jiang H.,Sichuan UniversityChengdu | Liu W.,Sichuan UniversityChengdu | Li G.,Sichuan UniversityChengdu | Fan T.,Sichuan UniversityChengdu | Mao B.,Sichuan UniversityChengdu
Alternative Therapies in Health and Medicine | Year: 2016

Context • Upper airway cough syndrome (UACS), previously called postnasal drip syndrome (PNDS), has been considered universally to be one of the most common causes of chronic cough. As an important part of complementary and alternative therapy, traditional Chinese medicine (TCM) has found an exact curative therapy for chronic cough through clinical practice for thousands of years. Objective • The aim of the current review was to investigate systematically the beneficial and adverse effects of Chinese medicinal herbs (CMH) in the treatment of UACS. Design • The research team performed searches in 11 main databases from respective inception to October 31, 2015, supplemented with manual retrieval of other data. Only randomized, controlled trials (RCTs) reporting on the effectiveness of CMH in patients with UACS were included. Descriptive and quantitative data on the studies’ designs, population demographics, interventions, outcomes, and methodological quality were extracted and tabulated. Methodological quality was assessed using the Cochrane risk-of-bias system and the quality of the evidence was evaluated using the Grades of Recommendation Assessment, Development, and Evaluation (GRADE) system. Participants • The reviewed studies included 1355 participants—720 in the CMH groups and 635 in the control groups—of both genders, from various professional and ethnic groups, and with a wide range of ages. They all had a duration of cough symptoms of longer than 8 wk and a clinical diagnosis of chronic cough induced by UACS that was supported by appropriate physical findings. Outcome Measures • The primary outcomes included (1) TCM recovery rate and (2) TCM cough symptom score. TCM’s curative effect was calculated as the cumulative percentage of the symptom-score reduction (PSSR), estimated between baseline and postintervention. The cough symptom scores were graded according to the Chinese Criteria Guiding Principle of Clinical Research on New Drugs of TCM, with the scores being classified into 4 grades. Those scores ranged from 0–3 (ie, 0, 1, 2, 3, respectively), or 0–9 (ie, 0, 3, 6, 9, respectively), with the higher scores signifying a more frequent and severe cough. Results • A total of 16 studies that had been published in Chinese journals was ultimately identified for the review. The majority of methodological judgments demonstrated an unclear risk of bias. A meta-analysis was conducted using a random effects model due to the poor homogeneity of the studies. Compared with Western medicine (WM), patients in both the CMH groups and the integrated therapy groups showed (1) a higher TCM recovery rate; (2) better relief of primary symptoms, including cough and postnasal dripping; (3) a reduction in physical signs, including the cobblestone appearance of the oropharyngeal mucosa or mucus in the oropharynx; and (4) a lower risk of cough relapse. No severe adverse events occurred in either group. Conclusions • CMH may be a safe and effective alternative for the treatment of UACS. The study highlighted the paucity of reliable clinical evidence for CMH and the need for RCTs of higher quality in the future. © 2016, InnoVision Communications. All rights reserved.


Wang L.,Sichuan UniversityChengdu | Jin X.,Sichuan UniversityChengdu | Zhao X.,Sichuan UniversityChengdu | Liu D.,Sichuan UniversityChengdu | And 6 more authors.
Oral Diseases | Year: 2014

Focal dermal hypoplasia (FDH), or Goltz-Gorlin syndrome, is a rare syndrome and may result in multisystem disorders. Several reviews of FDH have been published. However, the last comprehensive review of this disorder appeared more than 20 years ago. To date, a number of new clinical manifestations have been reported and considerable knowledge has accumulated regarding etiology and pathogenetic mechanisms. The purpose of this review is to gather these more recent data and to provide organized and reliable information. So we reviewed 159 cases of FDH that had been reported from 1990 to 2012, summarized the new discoveries, and suggested a potential standard for the diagnosis of FDH. We also reported on a Chinese girl with FDH, who was clinically and histologically in accord with FDH, as an example. © 2013 John Wiley & Sons A/S.


Human respiratory syncytial virus (HRSV) is a major pathogen that causes worldwide seasonal epidemic disease in infants due to its genetic variations. However, published information on the molecular epidemiology of HRSV was never reported particularly in Chengdu of West China. During five consecutive seasons (from 2009 to 2014), 433 (23.7%) of 1827 samples from hospitalized patients were identified as HRSV positive. Epidemiological characteristics of HRSV revealed that subtype A viruses (62.7%) prevailed in the first three epidemic seasons and faded in the next two seasons, while subtype B viruses (37.3%) kept circulating in five epidemic periods. According to the phylogenetic analysis of glycoprotein (G) gene, five HRSV genotypes NA1, ON1, BA9, BA-C, and CB1 were found in Chengdu. The predominant circulating genotype changed from NA1 in the period of 2010-2012 to BA9 of 2013-2014. The newly emerging ON1 was first reported in West China in October 2013. The early genotypes BA-C and CB1 were replaced by the prevailing BA9 after the third epidemic peak. Genetic mutations in glycosylation sites of G protein were found in HRSV variants, suggesting the virus is able to escape the immune recognition and attack. This study elucidated the local HRSV epidemic was associated with the alternate circulation of multiple genotypes and with the change of glycosylation sites of G protein. © 2016 Wiley Periodicals, Inc.


Daji W.,Sichuan UniversityChengdu | Dachuan Z.,Sichuan UniversityChengdu | Dachuan Z.,PLA Logistical Engineering University | Huafeng L.,PLA Logistical Engineering University | Guoxu C.,PLA Logistical Engineering University
Tribology Transactions | Year: 2015

Muscovite/La2O3 composite powders were prepared by ball-milling solid-state chemical reaction at room temperature. The phase composition and micromorphology of the composite powders were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). The tribological properties of different samples were tested and compared using four-ball wear testing on an MMW-1A multifunctional friction and wear testing machine. The SEM micrography and energy spectrum of the composite powders illustrated that La2O3 particles were coated on the surface of muscovite particles. The results of the friction tests indicated that lubrication oil with muscovite/La2O3 composite powders presents better friction reducing and antiwear properties than that of the base oil, and the friction coefficients and diameters of wear scars decreased by 47.6 and 11.2% using 500SN base oil with 0.6g/L of muscovite/La2O3 composite powders as additives, respectively. The composite powders with 5 wt% La2O3 present the best comprehensive tribological properties. The micromorphology and chemical composition of the worn surface were analyzed by SEM and EDX, which confirm that the composite powders directly participate in the complicated physicochemical process of reactions on the worn surfaces, therefore improving the tribological properties of the base oil. © 2015, Copyright © Society of Tribologists and Lubrication Engineers.


Chen C.,Sichuan UniversityChengdu | Gu S.,Sichuan UniversityChengdu | Jiang X.,Sichuan UniversityChengdu | Zhang Z.,Sichuan UniversityChengdu
Environmental Toxicology | Year: 2015

Arsenite is a certainly apoptosis inducer in various cell types. However, the detailed mechanism underlying how arsenite trigger apoptosis remains elusive. In this study, using human bronchial epithelial cell as a culture system, we demonstrated that arsenite-induced nuclear translocation of nuclear factor kappa B (NF-κB) resulted in the release of cytochrome c, the modulation of Fas and FasL, caspase activation, and ultimately leading to cell apoptosis. Importantly, we showed for the first time that the NF-κB-mediated apoptosis induced by arsenite was regulated by G protein-adenylate cyclase (AC)-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway. Inhibition of this classical G protein signaling pathway by a typical PKA inhibitor, H-89, caused the inactivation of NF-κB, the depletion of caspase-3, 8 and 9 activities, and thus reducing the level of cell apoptosis. Taken together, our results indicate that arsenite is able to trigger cell apoptosis in human bronchial epithelial cells through the nuclear translocation of NF-κB, which can be modulated by G protein signaling pathway. These findings further suggest that inhibition of G protein-mediated pathway by specific inhibitors may be a potential strategy for the prevention of arsenite toxicity. © 2015 Wiley Periodicals, Inc.


PubMed | Sichuan UniversityChengdu and Chengdu University of Technology
Type: Case Reports | Journal: International journal of clinical and experimental pathology | Year: 2016

Alveolar rhabdomyosarcoma (ARMS) is a common soft tissue tumor in children which can rarely metastasize to the breast in adults. Here we report the rare case of a 42-year-old Asian woman, who was diagnosed with ARMS of the nasopharynx and paranasal sinuses, and got a complete remission (CR) after surgery and chemoradiotherapy. Then the patient relapsed in the unilateral breast seventeen months later. Histology and immunohistochemistry of the primary sites and the breast lesions, combined with FISH, have been performed to confirm the diagnosis of metastatic alveolar rhabdomyosarcoma. With a rational therapeutic regimen of surgery, chemotherapy and radiotherapy, the patient has got a complete remission again.


PubMed | Sichuan UniversityChengdu and Chengdu University of Technology
Type: | Journal: Frontiers in cellular and infection microbiology | Year: 2016

Candida leukoplakia (OLK) is a kind of oral leukoplakia combined with chronic candidal infection, which plays an important role in the malignant transformation of OLK. However, little is known about the etiology, including susceptibility of leukoplakia to candidal adhesion, invasion and infection. Some antimicrobial peptides secreted by oral epithelial cells or fibroblasts potentially have antifungal activities against

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