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Zhang L.,Chinese Academy of Agricultural Sciences | Liu H.-B.,Chinese Academy of Agricultural Sciences | Gu J.-G.,Chinese Academy of Agricultural Sciences | Song Y.-F.,Chinese Academy of Agricultural Sciences | And 8 more authors.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban | Year: 2013

An agar plate antagonism experiment in combining with in vivo screening experiment was conducted to study the affinity and bacteriostasis spectrum of the combination of biocontrol agents Trichoderma longbrachitum and Streptomyces jingyangensis to Nicotiana tabacum seedlings, with the effects of each agent and their combination on the N. tabacum seedlings growth, induced resistance, and resistance to Phytophthora nicotianae analyzed. The two agents had no interactive inhibitory effect and showed higher affinity to N. tabacum, and the agents themselves as well as their metabolites had higher bacteriostasis activities and wider bacteriostasis spectrum to P. nicotiaonae, Pythium aphanidermatum, and Alternaria alternate in different habitats. The combination of the two agents affected the morphological characteristics of the seedlings underground and aboveground parts, promoted the growth of root, stem, and leaf, and increased the root volume, total surface area, length, and average diameter as well as the stem height and size and the leaf length, width, and biomass, with these promotion effects being superior than those of the single-agent treatment. The combination of the two agents also increased the activities of the defensive enzymes superoxide dismutase, catalase, phenylalanine ammonia lyase, and peroxidase in the seedlings root significantly, with the relative control efficiency against P. nicotianae reached 69.3%, as compared to the conventional treatment. This study showed that the combination of T. longbrachitum and S. jingyangensis was a compatible combination with higher affinity and efficiency. This combination showed a synergistic effect of the two agents in plant disease control and in promoting plant growth, being able to promote the tobacco seedlings growth and control the P. nicotianae effectively.


Zhang L.,Chinese Academy of Agricultural Sciences | Zhang L.,University of Chinese Academy of Sciences | Liu H.-B.,Chinese Academy of Agricultural Sciences | Gu J.-G.,Chinese Academy of Agricultural Sciences | And 12 more authors.
Chinese Journal of Applied Ecology | Year: 2013

An agar plate antagonism experiment in combining with in vivo screening experiment was conducted to study the affinity and bacteriostasis spectrum of the combination of biocontrol agents Trichoderma longbrachitum and Streptomyces jingyangensis to Nicotiana tabacum seedlings, with the effects of each agent and their combination on the N. tabacum seedlings growth, induced resistance, and resistance to Phytophthora nicotianae analyzed. The two agents had no interactive inhibitory effect and showed higher affinity to N. tabacum, and the agents themselves as well as their metabolites had higher bacteriostasis activities and wider bacteriostasis spectrum to P. nicotianae, Pythium aphanidermatum, and Alternaria alternate in different habitats. The combination of the two agents affected the morphological characteristics of the seedlings underground and aboveground parts, promoted the growth of root, stem, and leaf, and increased the root volume, total surface area, length, and average diameter as well as the stem height and size and the leaf length, width, and biomass, with these promotion effects being superior than those of the single-agent treatment. The combination of the two agents also increased the activities of the defensive enzymes superoxide dismutase, catalase, phenylalanine ammonia lyase, and peroxidase in the seedlings root significantly, with the relative control efficiency against P. nicotianae reached 69.3%, as compared to the conventional treatment. This study showed that the combination of T. longbrachitum and S. jingyangensis was a compatible combination with higher affinity and efficiency. This combination showed a synergistic effect of the two agents in plant disease control and in promoting plant growth, being able to promote the tobacco seedlings growth and control the P. nicotianae effectively.


Zhang L.,Tobacco Research Institute of CAAS | Liu H.,Tobacco Research Institute of CAAS | Gu J.,Chinese Academy of Agricultural Sciences | Lei Q.,Sichuan Tobacco Monopoly Administration | And 4 more authors.
Tobacco Science and Technology | Year: 2013

In order to investigate the co-effects of compound microbial inoculum and organic-inorganic fertilizer on the resistance, growth, yield and quality of flue-cured tobacco, the activity of various defensive enzymes in tobacco roots and leaves at different growing stages as well as the changes of tobacco yield and quality were determined with field trials. The results indicated that proportionate application of compound microbial inoculum with organic and inorganic fertilizers significantly improved the agronomic character of tobacco, enhanced the activities of defensive enzymes, such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and phenylalanine ammonia lyase (PAL) in tobacco roots and leaves, and reduced the morbidity and disease index relative to tobacco black shank disease, tobacco mosaic virus disease and tobacco brown spot disease. Correlation analysis showed that the activities of defensive enzymes (PAL, SOD, and POD) in tobacco roots positively correlated to those in tobacco leaves. Proportionate application increased the contents of total sugar and reducing sugar in tobacco leaves, especially increased potassium content significantly, which made the chemical composition in tobacco leaves more harmonious. The experiments indicated that the application of compound microbial inoculum together with 75% organic and inorganic fertilizers was beneficial to the growth, development of flue-cured tobacco and increasing its yield, quality and resistance to stress.

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