Sichuan Provincial Peoples Hospital

Chengdu, China

Sichuan Provincial Peoples Hospital

Chengdu, China
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AIM: Tyrosine kinase receptor-B (TrkB), a high-affinity receptor for brain-derived neurotrophic factor (BDNF), functions via specific binding with the BDNF. Although studies have shown that BDNF promotes the expression of glutamate transporter-1 (GLT-1) in the glial cells during neurodegeneration, evidence is lacking regarding whether BDNF/TrkB pathway is associated with the abnormal function of GLT-1.The aim of this study is to analyze the expressions of TrkB and GLT-1 in the brain of pentylenetetrazol (PTZ) kindling model of chronic epilepsy in rats, and to provide evidence for the correlation between the BDNF/TrkB pathway and GLT-1 and clues for the prevention and treatment targets of epilepsy.MATERIAL AND METHODS: In total, 40 Sprague-Dawley rats were randomly classified as model (n= 30) and control (n= 10) groups respectively. Western blotting was used for analyzing the expression of TrkB and GLT-1, and double immunofluorescence staining was used for detecting the cells positive for both glial fibrillary acidic protein (GFAP) and TrkB in the hippocampus and temporal cortex 7 days after establishing the kindling model.RESULTS: Compared with the control group, protein level expression of TrkB and GLT-1, and mean optical density (OD) value of the cells positive for both GFAP and TrkB in the hippocampus and temporal cortex were significantly increased after the onset of epilepsy (P < 0.05).CONCLUSION: The BDNF/TrkB pathway may participate in the epileptogenesis through modulating the biological effect of GLT-1 in the glial cells of PTZ kindling rats, though the specific mechanisms require further investigations.


He X.,Sichuan Provincial Peoples Hospital
Journal of Clinical Dermatology | Year: 2017

Objective: To detect the levels of thyroxin and thyroid autoantibodies in patients with alopecia areata(AA). Methods: Chemiluminescenee immunoassay was used to measure the levels of free triiodothyronin(FT3), free thyronxine(FT4), thyrotrophin(TSH), thyroglobulin antibody(TGAh) and thyroperoxidase antibody (TPOAb) in 111 patients with AA and 85 healthy controls. The positive rates of thyroid antibodies were compared between AA and normal controls. Results: In comparison with normal controls, the levels of serum FT3 and FT4 were higher while the levels of TSH were lower in patients with AA, but no significant differences for either of them. TGAb positive rate was no significant differences between AA group and normal controls. In contrast, positive rate of TPOAb in patients with AA was significantly higher than that in normal controls(P<0.05). The positive rates of neither TGAb nor TPOAb differed significantly between the mild and severe AA group, or between the recurrent and non-recurrent AA group. The positive rate of TPOAb was higher in female patients than in male patients(P<0.05). Conclusion: The positive rate of TPOAb is significantly higher in patients with AA, and higher in female patients than in male patients.


Zhang J.,Sichuan Provincial Peoples Hospital
Latin American Journal of Pharmacy | Year: 2017

Irinotecan is the first-line anti-lung cancer drug, and irinotecan undergoes two-step metabolic reaction to form the glucuronide of SN-38 after administration of irinotecan. Ticagrelor is a platelet aggregation inhibitor produced by AstraZeneca, and approximately 1/3 of ticagrelor is metabolized to form its active metabolite AR-C124910. Irinotecan-ticagrelor interaction was evaluated through determining the inhibition of AR-C124910 on the glucuronidation of SN-38 using in vitro human liver microsomes (HLMs) incubation system. Initial screening of the inhibition of AR-C124910 on the glucuronidation of SN-38 showed that 100 μM of AR-C124910 significantly inhibited the glucuronidation metabolism of SN-38 (p < 0.01). Furthermore, concentration-dependent inhibition of AR-C124910 on the glucuronidation of SN-38 was demonstrated, and the IC50 value was calculated to be 12.5 μM. Lineweaver-Burk plot showed that AR-C124910 competitively inhibited SN-38 glucuronidation. Using the second plot, the inhibition kinetic parameter (Ki) was calculated to be 32.7 μM. In conclusion, ticagrelor is firstly metabolized to form its active metabolite AR-C124910, and AR-C124910 strongly inhibited the glucuronidation of SN-38. Therefore, potential ticagrelor-irinotecan interaction was demonstrated in this study through evaluating the inhibition of AR-C124910 on the glucuronidation of SN-38. © 2017, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.


Yuan K.,University of North Dakota | Yuan K.,University of Sichuan | Xie K.,Sichuan Provincial Peoples Hospital | Fox J.,University of North Dakota | And 4 more authors.
Gastroenterology | Year: 2013

Background & Aims Little is known about functions of microRNA (miR) passenger strands (miR*) or their roles in tumor development or progression. We screened for miRs and miR* with levels that were altered in metastatic colorectal cancer (CRC) cells and human tumor samples and investigated their targets and effects on cell function and tumor progression in mice. Methods We performed array-based profile analysis to identify miRs with levels that were increased more than 2-fold in metastatic (SW620) CRC cells compared with nonmetastatic (SW480) cells. Quantitative polymerase chain reaction and in situ hybridization analyses were used to measure miRNA levels in CRC cell lines and human tumor samples. We used miRNA duplex mimics or inhibitors to increase and decrease levels of miRNA in CRC cells and assessed their activities and ability to form metastatic xenograft tumors in nude mice. Results Levels of miR-221* and miR-224 were reduced in metastatic compared with nonmetastatic CRC cells; levels in human tumor samples correlated inversely with tumor stage and metastasis to lymph nodes as well as patient survival times. SW480 cells transfected with miR-221* or miR-224 inhibitors had increased motility in vitro compared with SW480 control cells and formed larger, more metastatic tumors when injected into mice. SW620 cells transfected with miR-221* or miR-224 mimics had reduced migration and motility in vitro and formed smaller tumors with fewer metastases in mice compared with control SW620 cells. We identified the 3′ untranslated region of MBD2 messenger RNA as a target of miR-221* and miR-224. MBD2 silences the gene encoding maspin, a suppressor of metastasis. In CRC cells, we found that miR-221* and miR-224 increase the expression of maspin through MBD2 down-regulation. Conclusions In metastatic CRC cells, reduced levels of miR-221* and miR-224 increase levels of MBD2, thereby decreasing expression of the metastasis suppressor maspin. Increased activities of miR-221* and miR-224 reduce growth and metastasis of CRC xenograft tumors in mice; these miRs might be developed as therapeutic reagents or biomarkers of CRC progression. © 2013 by the AGA Institute.


Hu T.,Sichuan Provincial Peoples Hospital | Lu Y.-R.,Sichuan Provincial Peoples Hospital
Cancer Cell International | Year: 2015

Background: Long non-coding RNAs (lncRNAs) are increasingly implicated in the regulation of the progression of malignancy. Aim: To clarify the relations among BCYRN1 (brain cytoplasmic RNA 1, a long non-coding RNA), c-MYC and cell metastasis of non-small-cell lung cancer (NSCLC). Methods: Real-time PCR was used to measure expression of BCYRN1 in NSCLC. Knockdown and overexpression of c-MYC were respectively performed using shRNA and lentivirus to investigate its effect on BCYRN1 expression. BCYRN1 was respectively knockdown and overexpressed by siRNA and BCYRN1 mimics to investigate its role in regulating cell metastasis in vitro. ChIP (chromatin immunoprecipitation) assay was performed to confirm the binding of c-MYC to the promoter of BCYRN1. Expression levels of matrix metalloproteinases (MMP9 and MMP13) were determined using real-time PCR and Western blotting. Results: BCYRN1 is upregulated and targeted by c-MYC in NSCLC, leading to the increase of cell motility and invasiveness. RNA interference and lentivirus infection showed a positive correlation between the expressions of c-MYC and BCYRN1. ChIP assay confirmed the binding of c-MYC to the promoter region of BCYRN1 gene. In-vitro cell metastasis experiments demonstrated that BCYRN1 was necessary in the c-MYC-regulated cell migration and invasion. The mRNA and protein expression levels of MMP9 and MMP13 descended with the decreasing BCYRN1 level and ascended with the upregulation of BCYRN1. Conclusion: These findings uncover a regulatory mechanism in NSCLC cells involving the metastasis-promoting lncRNA BCYRN1 that improves expressions of the key metastasis-supporting proteins MMP9 and MMP13. © Hu and Lu; licensee BioMed Central.


Yang C.,Sichuan Provincial Peoples Hospital | Pan Y.,Sichuan Provincial Peoples Hospital
Tumor Biology | Year: 2015

The molecular mechanisms underlying the anti-cancer effects of chemotherapy drugs are not completely understood. Here, we studied the effects of fluorouracil (5-FU) on gastric carcinoma (GC) cells. We found that 5-FU dose-dependently inhibited the growth of GC cells, in either a cell counting kit-8 (CCK-8) assay or a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Moreover, autophagy-associated protein 6 (ATG6) or Beclin-1 was dose-dependently activated by 5-FU in GC cells. Further, microRNA (miR)-30 was found to be regulated by 5-FU, and bioinformatics analysis showed that miR-30 targeted the 3′-UTR of Beclin-1 to inhibit its translation. Together, these data suggest that 5-FU may suppress miR-30 to upregulate Beclin-1 to induce autophagic cell death and cell proliferation arrest in GC cells. © 2015 International Society of Oncology and BioMarkers (ISOBM)


Peng Q.,Sichuan Provincial Peoples Hospital
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2013

Kawasaki disease (KD) is a common autoimmune vasculitis. It has been regarded as the leading cause of acquired heart disease in children. This study aimed to assess the relationship between genome-wide association study (GWAS)-linked gene loci and KD. By March of 2013, the published GWAS literatures of KD were retrieved from the databases including PubMed, MEDLINE, Web of Science, Cochrane Library, CNKI, VIP and Wanfang, and the gene loci associated with KD at genome-wide significance of P < 5.0×10(-8) were determined. For each of the gene loci, one single-nucleotide polymorphism (SNP) with strong association with KD was chosen for meta-analysis. Then the published case-control studies reporting the associations of the SNPs with KD were collected from English and Chinese databases with the same criteria. The Meta-analyses were conducted with RevMan 5.1 software after screening and evaluation. A total of 4 gene loci including FCGR2A, BLK, CD40 and HLA were observed having association with KD at genome-wide significance of P < 5.0×10(-8) in at least one GWAS. The risk alleles of the SNPs in the gene loci were all more common in patients with KD relative to controls in the systematic reviews with 8, 4, 6 and 4 extracted case-control studies, respectively[ FCGR2A rs1801274: P < 0.001, OR = 1.40, 95% CI (1.30, 1.51); BLK rs2254546: P < 0.001, OR = 1.69, 95% CI (1.52, 1.88); CD40 rs4813003: P < 0.001, OR = 1.31, 95% CI (1.22, 1.41); HLA rs2857151: P < 0.001, OR = 1.41, 95% CI (1.27, 1.57)]. The significant publication bias was not observed in the meta-analyses. Our results confirmed the overall association of the 4 gene loci with KD in observed populations, together with the consistent presence of the relationship between BLK or HLA and KD in the populations, suggesting that it is hopeful to find the genetic marker combination predicting the risk of KD, the formation of secondary coronary artery lesions and the resistance of intravenous immunoglobulin, by further seeking the function SNPs of the gene loci and investigating the effect on the important clinical phenotypes of KD.


Gu Q.,Sichuan Provincial Peoples Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To investigate the diagnostic and therapeutic methods of orbital apex syndrome caused by sinus diseases and to achieve earlier diagnosis and timely treatment. Clinical data of 6 cases in our department from January 2008 to March 2012 were retrospectively analyzed. All cases had surgical treatment. All cases were preoperatively diagnosed by CT, MRI or intranasal endoscopy. The postoperative pathology showed 2 cases with fungal sphenoiditis, 2 cases with ethmoid mucocele and 2 cases with sphenoid mucocele. Orbital apex syndrome caused by sinus diseases is very rare. The possible causes of misdiagnosis are low incidence of the disease, nonspecific eye symptoms, and unawareness of the doctor, especially ophthalmologist. CT, MRI or intranasal endoscopy can greatly improve the diagnosis of the disease. Endoscopic sinus surgery is a safe and effective method for orbital apex syndrome caused by sinus diseases,which is the primary therapy for the disease.


Zhu J.,Sichuan Provincial Peoples Hospital
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2011

To study the effectiveness of avascular necrosis of the femoral head treated by lesions clearance, Between March 2008 and May 2010, 14 patients (16 compact bone grafting, and porous tantalum rod implantation. hips) with avascular necrosis of the femoral head were treated by lesions clearance, compact bone grafting, and implantation of porous tantalum rod. Of 15 cases, 13 were male (15 hips) and 1 was female (1 hip) with a median age of 42.2 years (range, 18-73 years), including traumatic in 1 case (1 hip), alcoholic in 4 cases (4 hips), and steroid-induced in 9 cases (11 hips); 3 hips were at Association Research Circulation Osseous (ARCO) stage I and 13 hips were at ARCO stage II. The Harris score was 51.89 +/- 12.42, and the X-ray score was 31.88 +/- 4.03. All the cases were diagnosed by X-ray films and MRI. The median disease duration was 2.5 years (range, 6 months to 7 years). All the patients accepted the operation of lesions clearance by slotting at the neck of femur, then, compact bone grafting, and implantation of porous tantalum rod were performed. The affected limb could not bear weight loading at 1-3 months after operation and partly bear weight loading after 3 months of operation. Primary healing of incision was achieved in all patients and no complication occurred. The patients were followed up 24 months on average (range, 13-36 months). Two patients underwent total hip arthroplasty at 4 months and 2 years respectively because of even worsened pain and collapsed femoral heads; 12 patients achieved obvious pain relief with a survival rate 87.5% (14/16). The postoperative Harris score was 84.89 +/- 17.96, showing significant difference when compared with preoperative score (t = -8.038, P = 0.001). The X-ray examination showed definite ossification, increased density, regular arrangement of the trabeculae and no collapsed femoral head. The X-ray score was 32.19 +/- 6.57, showing no significant difference when compared with preoperative score (t = -2.237, P = 0.819). Lesions clearance, compact bone grafting, and implantation of porous tantalum rod for avascular necrosis of the femoral head have a good short-term clinical result.


Wu B.,Sichuan Provincial Peoples Hospital
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2013

To investigate the administration of far lateral craniocervical approach in the jugular foramen (JF) tumors. A retrospective analysis was performed in 14 cases of JF tumors (9 neurilemmomas, 3 meningiomas, 1 glomus jugulare tumor, and 1 adenoid cystic carcinoma) surgically treated between January 2009 and January 2012, with focus on the surgical approach. Six patients (6/14) showed hydrocephalus. The tumor type was composed of 5 intracranial and intraforamen tumors with patent or occluded jugular bulb, 1 intracranial tumor with extension into the upper cervical canal, 4 extracranial and intra foramen tumors, 4 intra- and extracranial dumbbell-shaped communicating tumors involving the parapharyngeal space above C2 or extending caudally below C3. Far lateral postcondylar approach (FLPC) was carried out in 2 cases, far lateral tansjugular process approach (FLTJP) in 3 cases, combined FLPC + C1-2 semi-laminectomy approach in 1 case, combined FLTJP + trans-C1 transverse process approach in 7 cases, and combined FLTJP + neck approach with dissection of carotid sheath to the skull base in 1 case. Endovascular embolotherapy prior to surgical resection was performed in 1 glomus jugulare tumor. Total tumor removal was achieved in 12 patients and subtotal removal in 2 patients, with no cerebrospinal fluid leakage or operative mortality. New cranial nerve paresis occurred after surgery in 1 case of facial nerve and 1 case of lower cranial nerve. Transient worsening of preoperative lower cranial nerve deficits was noted in 3 patients. Long-term follow-up study ranging from 5 to 32 months (average 13.7 months) showed 7 patients with lower cranial nerve deficits (6 preexisting and 1 new), with exception of one preoperative lower cranial nerve dysfunction due to the infiltration of an adenoid cystic carcinoma, experienced favorable improvement with recovery of adequate swallowing function, but voice disturbance remained in 4 cases. One patient with new facial nerve deficit presented with partial improvement and the hydrocephalus in 6 patients all spontaneously regressed. There was no tumor recurrence in patients receiving total removal and no tumor progression in patients undergoing subtotal removal. FLTJP is a basic approach for JF tumors. The combined cranial and cervical approach should be considered in those tumors extending into the upper cervical canal and parapharyngeal space. The associated hydrocephalus seldom requires additional surgical management.

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