Sichuan Provincial Orthopedics Hospital

Chengdu, China

Sichuan Provincial Orthopedics Hospital

Chengdu, China
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Tao Z.-S.,Wenzhou University | Zhou W.-S.,Guizhou University | Tu K.-K.,Wenzhou University | Huang Z.-L.,Wenzhou University | And 5 more authors.
Injury | Year: 2015

In this study, we tested the effect of Teriparatide (PTH) in combination with β-tricalcium phosphate (β-TCP) as a bone void filler in an ovariectomised rat distal femoral metaphysis model.β-TCP is a completely resorbable synthetic calcium phosphate and the Teriparatide is a drug that can promote bone formation in the condition of osteoporosis. A critical size defect of 3 mm in diameter, a through-hole bone defect, was drilled into each distal femur of the ovariectomised rats. The hole was filled with β-TCP and the rat was injected PTH Teriparatide (30 μg/kg) in peritoneum 5 times per week. After 4and 8 weeks the animals were killed and the degree of bone healing analysed. In total, 60 animals were investigated. When the β-TCP and PTH were used, histological, biochemistry and histomor-phometric evaluations revealed significantly better bone healing in terms of quantity and quality of the newly formed bone. The Ovariectomised rats which suffer from femur metaphysis defect are cured by embedding β-tricalcuim phosphate and intermittently cured by parathyroid hormone (PTH). © 2015 Elsevier Ltd. All rights reserved.


Tao Z.-S.,Wenzhou University | Zhou W.-S.,Guizhou University | Tu K.-K.,Wenzhou University | Huang Z.-L.,Wenzhou University | And 5 more authors.
Injury | Year: 2015

The effect of human parathyroid hormone 1-34 (PTH) and simvastatin (SIM) alone could promote bone healing in osteoporotic osseous integration of the implant, but there are no reports about the combined use of PTH and SIM for promotion of bone healing around implant in osteoporotic settings still limited. This study aims to investigate effects of PTH + SIM on osseous integration of the implant in OVX rats. Female Sprague-Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group control; group SIM; group PTH and group PTH + SIM. Afterwards, all OVX rats received hydroxyapatite (HA)-coated titanium rods (external diameter and length are 1.5 mm and 20 mm) in the femoral medullary canal. Subsequently, the animals from group SIM, group PTH and group PTH + SIM received human parathyroid hormone 1-34 (60 μg/kg, three times a week), SIM (5 mg/kg daily), or both for 12 weeks. Implants were inserted bilaterally in all animals until death at 12 weeks. The bilateral femurs of rats were harvested for evaluation. All groups increased new bone formation around the surface of titanium rods and push-out force; group PTH + SIM showed the strongest effects on new bone formation and biomechanical strength. Additionally, these are significant difference observed in bone formation and push-out force between groups SIM and PTH. This finding suggests that intermittent administration of PTH or SIM alone has an effect to increase new bone formation on the surface of HA-coated implants in the osteoporotic condition, and the additive effects of combination PTH and SIM on osseous integration of the implant in OVX rats. © 2015 Elsevier Ltd. All rights reserved.


PubMed | Wenzhou University, Guizhou University, Wenzhou Central Hospital and Sichuan Provincial Orthopedics Hospital
Type: Journal Article | Journal: Journal of biomaterials applications | Year: 2016

Previous studies have demonstrated the effect of human parathyroid hormone (1-34) (PTH) or strontium-doped hydroxyapatite coating (Sr-HA) on osteoporotic bone implantation. However, reports about effects of PTH plus Sr-HA on bone osseointegration of titanium implants in a state of osteoporosis were limited. This study was designed to investigate the effects of intermittent administration of human parathyroid hormone (1-34) on strontium-doped hydroxyapatite coating (Sr-HA) implant fixation in ovariectomized (OVX) rats. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups including control group, Sr group, PTH group and PTH+Sr group. Forty OVX rats accepted implant insertion in the distal femurs, control group, and PTH group with HA implants and the Sr group and PTH+Sr group with Sr-HA implants. Animals from PTH group and PTH+Sr group then randomly received PTH (60g/kg, 3 times a week) until death at 12 weeks. After 12-week healing period, implants from group PTH+Sr revealed improved osseointegration compared with other treatment groups, which is manifested by the exceeding increase of bone area ratio and bone-to-implant contact, the trabecular microarchitecture and the maximal push-out force displayed by tests like histomorphometry, micro-CT, and biomechanics evaluation. These results demonstrated that PTH+ Sr-HA coatings could enhance implant osseointegration in OVX rats, and suggested the feasibility of using this method to improve implant fixation in osteoporotic bone.


PubMed | Wenzhou Central Hospital, Jingmen No1 Peoples Hospital, Hangzhou Bay Hospital of Ningbo, Wenzhou University and 2 more.
Type: Journal Article | Journal: Medical & biological engineering & computing | Year: 2016

Surface modification techniques have been applied to generate titanium implant surfaces that promote osseointegration for the implants in cementless arthroplasty. However, its effect is not sufficient for osteoporotic bone. Strontium (Sr) promotes osteoblast proliferation and inhibits osteoclast proliferation and positively affects bone regeneration. The aim of this study was to confirm the effects of strontium-substituted hydroxyapatite (Sr-HA) coating via electrochemical deposition on implants osseointegration in the osteoporotic condition. Female Sprague Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group HA; group 5% Sr-HA; group 10% Sr-HA; and group 20% Sr-HA. Afterward, all rats from groups HA, 5% Sr-HA, 10% Sr-HA, and 20% Sr-HA received implants with hydroxyapatite coating containing 0, 5, 10, and 20% Sr. Implants were inserted bilaterally in all animals until death at 12weeks. The bilateral femurs of rats were harvested for evaluation. All treatment groups increased new bone formation around the surface of titanium rods and push-out force; group 20% Sr-HA showed the strongest effects on new bone formation and biomechanical strength. Additionally, these are significant differences in bone formation and push-out force was observed between groups 5% Sr-HA and 10% Sr-HA. This finding suggests that Sr-HA coating can improve implant osseointegration, and the 20% Sr coating exhibited the best properties for implant osseointegration among the tested coatings in osteoporosis rats.


PubMed | Wenzhou University, Guizhou University, Wenzhou Central Hospital and Sichuan Provincial Orthopedics Hospital
Type: Journal Article | Journal: Injury | Year: 2015

In this study, we tested the effect of Teriparatide (PTH) in combination with -tricalcium phosphate (-TCP) as a bone void filler in an ovariectomised rat distal femoral metaphysis model.-TCP is a completely resorbable synthetic calcium phosphate and the Teriparatide is a drug that can promote bone formation in the condition of osteoporosis. A critical size defect of 3mm in diameter, a through-hole bone defect, was drilled into each distal femur of the ovariectomised rats. The hole was filled with -TCP and the rat was injected PTH Teriparatide (30g/kg) in peritoneum 5 times per week. After 4and 8 weeks the animals were killed and the degree of bone healing analysed. In total, 60 animals were investigated. When the -TCP and PTH were used, histological, biochemistry and histomor-phometric evaluations revealed significantly better bone healing in terms of quantity and quality of the newly formed bone. The Ovariectomised rats which suffer from femur metaphysis defect are cured by embedding -tricalcuim phosphate and intermittently cured by parathyroid hormone (PTH).


PubMed | Wenzhou University, Guizhou University, Wenzhou Central Hospital and Sichuan Provincial Orthopedics Hospital
Type: Journal Article | Journal: Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery | Year: 2015

The objective of this study was to evaluate the effect of following combined treatment with parathyroid hormone (1-34) (PTH) and -tricalcium phosphate (-TCP) on local bone formation in a rat 3-mm critical-sized defect at the distal femur. Fourteen weeks were allowed to pass before defect surgery for the establishment of osteopenic animal models chronically fed a low-protein diet. All animals were randomly divided into four groups: group PTH; group -TCP, group PTH+-TCP, and a control group. All rats then underwent a surgical procedure to create bone defects in the bilateral distal femurs, and -TCP was implanted into critical-sized defects for the groups designated as -TCP and group PTH+-TCP. After the defect operation, all animals from group PTH and group PTH+-TCP received following subcutaneous injections with PTH (60g/kg, three times per week) until euthanasia at 4 and 8 weeks. The distal femurs and blood were collected for evaluation. The results of study showed the strongest effect on accelerating the local bone formation with treatment -TCP and PTH at 4 weeks and 8 weeks. The results from our study demonstrate that a combination of PTH and -TCP had an additive effect on local bone formation in osteopenic rats chronically fed a low-protein diet.


PubMed | Wenzhou University, Wenzhou Central Hospital and Sichuan Provincial Orthopedics Hospital
Type: Journal Article | Journal: Medical & biological engineering & computing | Year: 2016

The objective of this study was to evaluate the effect of following combined treatment with parathyroid hormone (1-34) (PTH) and beta-tricalcium phosphate (-TCP) on local bone formation in a rat 3-mm critical-size defect at distal femur. Twelve weeks after bilateral ovariectomy (OVX) and sham operation (sham), all animals were randomly divided into four groups: group OVX, group OVX+-TCP, group sham, and group sham+-TCP, then all rats underwent bone defect in the bilateral distal femurs, and -TCP were implanted into critical-sized defects for group OVX+-TCP and group sham+-TCP. After defect operation, all animals were received following subcutaneous injections with PTH (60g/kg, three times a week) until death at 4 and 8weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that systemic usage of PTH or local usage of -TCP can increase the healing of defects in OVX or sham rats. Furthermore, treatments with PTH and -TCP showed a stronger effect on accelerating the local bone formation than used alone. Osteoporosis can limit the function of PTH and/or -TCP. The results from our study demonstrate that combination of PTH and -TCP had an additive effect on local bone formation in non-osteoporosis and/or osteoporosis rats.


PubMed | Wenzhou University, Guizhou University, Wenzhou Central Hospital and Sichuan Provincial Orthopedics Hospital
Type: Journal Article | Journal: Injury | Year: 2015

The effect of human parathyroid hormone 1-34 (PTH) and simvastatin (SIM) alone could promote bone healing in osteoporotic osseous integration of the implant, but there are no reports about the combined use of PTH and SIM for promotion of bone healing around implant in osteoporotic settings still limited. This study aims to investigate effects of PTH+SIM on osseous integration of the implant in OVX rats. Female Sprague-Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group control; group SIM; group PTH and group PTH+SIM. Afterwards, all OVX rats received hydroxyapatite (HA)-coated titanium rods (external diameter and length are 1.5mm and 20mm) in the femoral medullary canal. Subsequently, the animals from group SIM, group PTH and group PTH+SIM received human parathyroid hormone 1-34 (60g/kg, three times a week), SIM (5mg/kg daily), or both for 12 weeks. Implants were inserted bilaterally in all animals until death at 12 weeks. The bilateral femurs of rats were harvested for evaluation. All groups increased new bone formation around the surface of titanium rods and push-out force; group PTH+SIM showed the strongest effects on new bone formation and biomechanical strength. Additionally, these are significant difference observed in bone formation and push-out force between groups SIM and PTH. This finding suggests that intermittent administration of PTH or SIM alone has an effect to increase new bone formation on the surface of HA-coated implants in the osteoporotic condition, and the additive effects of combination PTH and SIM on osseous integration of the implant in OVX rats.


PubMed | Wenzhou Central Hospital, Jingmen No 1 Peoples Hospital, Hangzhou Bay Hospital of Ningbo, Wenzhou University and 2 more.
Type: | Journal: Materials science & engineering. C, Materials for biological applications | Year: 2016

Surface modification techniques have been applied to generate titanium implant surfaces that promote osseointegration for the implants in cementless arthroplasty. However, its effect is not sufficient for osteoporotic bone. Zinc (Zn), magnesium (Mg), and strontium (Sr) present a beneficial effect on bone growth, and positively affect bone regeneration. The aim of this study was to confirm the different effects of the fixation strength of Zn, Mg, Sr-substituted hydroxyapatite-coated (Zn-HA-coated, Mg-HA-coated, Sr-HA-coated) titanium implants via electrochemical deposition in the osteoporotic condition. Female Sprague-Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group HA; group Zn-HA; group Mg-HA and group Sr-HA. Afterwards, all rats from groups HA, Zn-HA, Mg-HA and Sr-HA received implants with hydroxyapatite containing 0%, 10% Zn ions, 10% Mg ions, and 10% Sr ions. Implants were inserted bilaterally in all animals until death at 12 weeks. The bilateral femurs of rats were harvested for evaluation. All treatment groups increased new bone formation around the surface of titanium rods and push-out force; group Sr-HA showed the strongest effects on new bone formation and biomechanical strength. Additionally, there are significant differences in bone formation and push-out force was observed between groups Zn-HA and Mg-HA. This finding suggests that Zn, Mg, Sr-substituted hydroxyapatite coatings can improve implant osseointegration, and the 10% Sr coating exhibited the best properties for implant osseointegration among the tested coatings in osteoporosis rats.


PubMed | Wenzhou University, Guizhou University, Wenzhou Central Hospital and Sichuan Provincial Orthopedics Hospital
Type: Journal Article | Journal: Journal of materials science. Materials in medicine | Year: 2016

The effect of human parathyroid hormone 1-34 (PTH) and simvastatin (SIM) alone could promote bone healing in osteoporotic implant fixation, but there are no reports about the combined use of PTH and SIM for promotion of bone healing around implant in osteoporotic settings. This study aims to investigate effects of PTH+SIM on implant stabilization in osteopenic rats. Fourteen weeks after chronically fed a low protein diet, osteopenic rats randomly received implants. Subsequently, the animals were randomly divided into four groups: Control, SIM, PTH and PTH+SIM. Then all rats from groups PTH, SIM and PTH+SIM received PTH (40g/kg, three times a week), SIM (25mg/kg, daily), or both for 12weeks. The results of our study indicated that all treatments promoted bone healing around implant compared to Control, but PTH+SIM treatment showed significantly stronger effects than PTH or SIM alone in histological, micro-CT, and biomechanical tests. The results indicated additive effects of PTH and SIM on implant fixation in osteoporotic rats.

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