Yuan Z.-X.,Chinese Academy of Sciences |
Wu X.-J.,Integrative Traditional and Western Medicine Hospital of Sichuan Province |
Mo J.,University of Western Australia |
Wang Y.-L.,Sichuan Provincial Hospital for Women and Children |
And 2 more authors.
European Journal of Pharmaceutics and Biopharmaceutics | Year: 2015
We have previously demonstrated that peptide fragments (PFs) of the human serum albumin could be developed as potential renal targeting carriers, in particular, the peptide fragment, PF-A299-585 (A299-585 representing the amino acid sequence of the human serum albumin). In this paper, we conjugated triptolide (TP), the anti-inflammatory Chinese traditional medicine, to PF-A299-585 via a succinic acid spacer to give TPS-PF-A299-585 (TP loading 2.2% w/w). Compared with the free TP, TPS-PF-A299-585 exhibited comparable anti-inflammatory activity in the lipopolysaccharide stimulated MDCK cells, but was significantly less cytotoxic than the free drug. Accumulation of TPS-PF-A299-585 in the MDCK cells in vitro and in rodent kidneys in vivo was demonstrated using FITC-labeled TPS-PF-A299-585. Renal targeting was confirmed in vivo in a membranous nephropathic (MN) rodent model, where optical imaging and analyses of biochemical markers were combined to show that TPS-PF-A299-585 was capable of alleviating the characteristic symptoms of MN. The collective data affirm PF-A299-585 to be a useful carrier for targeting TP to the kidney. © 2015 Elsevier B.V. All rights reserved.
PubMed | University of Sichuan, Peking Union Medical College and Sichuan Provincial Hospital For Women and Children
Type: Journal Article | Journal: Stem cell research & therapy | Year: 2016
Liver disease is a major cause of death worldwide. Orthotropic liver transplantation (OLT) represents the only effective treatment for patients with liver failure, but the increasing demand for organs is unfortunately so great that its application is limited. Hepatocyte transplantation is a promising alternative to OLT for the treatment of some liver-based metabolic disorders or acute liver failure. Unfortunately, the lack of donor livers also makes it difficult to obtain enough viable hepatocytes for hepatocyte-based therapies. Currently, a fundamental solution to this key problem is still lacking. Here we show a novel non-transgenic protocol that facilitates the rapid generation of functional induced hepatocytes (iHeps) from human adipose-derived stem cells (hADSCs), providing a source of available cells for autologous hepatocytes to treat liver disease.We used collagenase digestion to isolate hADSCs. The surface marker was detected by flow cytometry. The multipotential differentiation potency was detected by induction into adipocytes, osteocytes, and chondrocytes. Passage 3-7 hADSCs were induced into iHeps using an induction culture system composed of small molecule compounds and cell factors.Primary cultured hADSCs presented a fusiform or polygon appearance that became fibroblast-like after passage 3. More than 95 % of the cells expressed the mesenchymal cell markers CD29, CD44, CD166, CD105, and CD90. hADSCs possessed multipotential differentiation towards adipocytes, osteocytes, and chondrocytes. We rapidly induced hADSCs into iHeps within 10 days in vitro; the cellular morphology changed from fusiform to close-connected cubiform, which was similar to hepatocytes. After induction, most of the iHeps co-expressed albumin and alpha-1 antitrypsin; they also expressed mature hepatocyte special genes and achieved the basic functions of hepatocyte. Moreover, iHep transplantation could improve the liver function of acute liver-injured NPG mice and prolong life.We isolated highly purified hADSCs and rapidly induced them into functional hepatocyte-like cells within 10 days. These results provide a source of available cells for autologous hepatocytes to treat liver disease.
Chen B.-Z.,Sichuan Provincial Hospital for Women and Children |
Tan L.,University of Sichuan |
Zhang L.,Sichuan Orthopaedic Hospital |
Shang Y.-C.,University of Sichuan
Journal of Clinical Anesthesia | Year: 2013
Study Objective: To test the hypothesis that muscle relaxant is not necessary in patients who are undergoing laparoscopic gynecological surgery with a ProSeal Laryngeal Mask Airway (ProSeal LMA™). Design: Prospective, randomized study. Setting: Operating room of Sichuan Provincial Hospital for Women and Children. Patients: 120 adult, ASA physical status 1 and 2 women, aged 18 to 55 years. Interventions and Measurements: Patients were randomly assigned to two groups (n=60) to receive a muscle relaxant (Group MR) or not (Group NMR). General anesthesia was used in patients of both groups for airway management with the ProSeal LMA. Peak airway inflation pressures, airway sealing pressure, minimum flow rate, and recovery time were assessed. Surgical conditions were assessed by the operating gynecologist. The frequency of sore throats was recorded. Main Results: ProSeal LMA insertion was 100% successful. Patients' lungs were ventilated with a maximum sealing pressure of 32 ± 5.1 cm H2O (Group MR) or 31 ± 4.9 cm H2O (Group NMR) (P = 0.341). The seal quality in both groups permitted the use of low flows: 485 ± 291 mL/min in Group MR and 539 ± 344 mL/min in Group NMR (P = 0.2). Surgical conditions were comparable between the two groups. There was no difference in the frequency of sore throats (20% vs 21.7%; P = 0.28). In Group NMR, there was a statistically significant reduction in recovery time versus Group MR (4.5 ± 2.6 min vs 10.3 ± 4.2 min; P < 0.01). Conclusion: Muscle relaxant is not necessary in general anesthesia with a ProSeal LMA. © 2013 Elsevier Inc. All Rights Reserved.
Zhuang L.,Sichuan Provincial Hospital for Women and Children |
Chen D.-X.,Sichuan Provincial Hospital for Women and Children |
Yao Y.-H.,Sichuan Provincial Hospital for Women and Children
Journal of Reproduction and Contraception | Year: 2013
Cesarean scar pregnancy (CSP) occurs when a gestation sac is implanted in the previous lower segment cesarean scar. The incidence of CSP is increasing worldwide. Uterine ateriovenous malformation (UAVM) is a rare gynecologic disease. Both of these diseases can cause severe vaginal bleeding and produce high morbidity rate. We describe a case of UAVM induced by a CSP. The patient suffered intermittent vaginal bleeding after two dilatation and curettage (D&C) one month before admission. The suspected diagnosis of CSP and UAVM were made after using transvaginal ultrasound with color Doppler and human chorionic gonadotropin (hCG) examination. Resection of the involved area including the cesarean scar and UAVM by laparotomy was performed successfully and the diagnosis was confirmed by the last pathologic result. © 2013 The Editorial Board of Journal of Reproduction and Contraception.
Tang F.,Sichuan Provincial Hospital for Women and Children |
Xiao B.,Sichuan Provincial Hospital for Women and Children |
Xiong Q.,Sichuan Provincial Hospital for Women and Children |
Yang M.,Sichuan Provincial Hospital for Women and Children
Journal of Obstetrics and Gynaecology Research | Year: 2010
Magnesium intoxication is a result of excessively high serum magnesium levels, which are generally represented by a patient's total serum magnesium concentration. This is the first case report in PubMed in which a female patient showed no signs of magnesium intoxication when her total serum magnesium concentration was 4.34 mg/dL, but showed signs of magnesium intoxication (i.e. absent-patellar reflex) when the concentration was 3.66 mg/dL. We concluded that the serum magnesium level cannot be represented by the total serum magnesium concentration to a full extent; a lower total serum magnesium concentration may still lead to magnesium intoxication. © 2010 Japan Society of Obstetrics and Gynecology.
Li S.-M.,Sichuan Provincial Hospital for Women and Children
Chinese Journal of Evidence-Based Medicine | Year: 2010
Objective: To investigate the application of hysteroscopy in pathological changes of infertility uterus. Methods: The clinical data of 226 cases of infertility females receiving hysteroscopy from January 2007 to June 2009 in Sichuan Provincial Hospital for Women and Children were retrospectively analyzed. Results: In 226 cases, hysteroscopic examination identified 147 cases of intrauterine diseases (65.04%), including 56 cases of intrauterine adhesion (24.78%), 32 cases of endometritis (14.16%), 27 cases of endometrial polyps (11.94%), 15 cases of uterine malformation (6.64%), 9 cases of submucous myoma (3.98%), 3 cases of endometrial tuberculosis (1.33%), 3 cases of uterus cavity narrow (1.33%), 2 cases of cervical internal relaxation (0.88%). No postoperative complications occurred, except for a small amount of vaginal bleeding. Conclusion: For the diagnosis of the pathological changes in uterus, hysteroscopy is a direct and accurate method with less operative duration, less trauma, less pain, quick recovery, no complications, and no necessity for hospitalization. It is worth to be popularized. © 2010 Editorial Board of Chin J Evid-based Med.
Wang L.-C.,University of Sichuan |
Chen E.-Q.,University of Sichuan |
Cao J.,Sichuan Provincial Hospital for Women and Children |
Liu L.,University of Sichuan |
And 6 more authors.
Hepatology International | Year: 2011
Purpose: Either combination treatment or monotherapy using agents with a high genetic barrier are recommended for hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB). The aim of this study was to compare effect of naïve HBeAg-negative CHB patients with either de novo combination of lamivudine (LAM) and adefovir dipivoxil (ADV) or entecavir (ETV) monotherapy. Methods: HBeAg-negative CHB patients (n = 71) with ALT levels between 2 and 10 times the upper normal limit and HBV DNA levels >104 copies/mL were enrolled. Patients were treated with either LAM 100 mg plus ADV 10 mg per day (n = 31) or ETV 0.5 mg per day (n = 40) for 48 weeks. Results: The average reduction in HBV DNA level compared with baseline were 5.16 ± 1.69 log in the LAM + ADV group and 5.36 ± 1.70 log in the ETV group by week 48 (P = 0.624). The virological response (VR) rates were 80.65 and 77.5%, the biochemical response (BR) rates were 93.55 and 90.00% at week 48 in the LAM + ADV and ETV groups, respectively. There was no significant difference in the VR and BR between the two groups. During the 48-week treatment period, virological breakthrough and serious side effects were not noted in any patient. Conclusions: Both LAM + ADV combination therapy and ETV monotherapy are effective in naïve HBeAg-negative CHB patients, but further studies are needed to obtain long-term results. © 2011 Asian Pacific Association for the Study of the Liver.
PubMed | Chongqing Medical University and Sichuan Provincial Hospital for Women and Children
Type: | Journal: BioMed research international | Year: 2016
Objective. Blood-brain barrier (BBB) is a key obstacle that prevents the medication from blood to the brain. Microbubble-enhanced cavitation by focused ultrasound can open the BBB and proves to be valuable in the brain drug delivery. The study aimed to explore the feasibility, efficacy, and safety of unilateral opening of BBB using diagnostic ultrasound targeted microbubbles destruction in rats. Methods. A transtemporal bone irradiation of diagnostic ultrasound and intravenous injection of lipid-coated microbubbles were performed at unilateral hemisphere. Pathological changes were monitored. Evans Blue extravasation grades, extraction from brain tissue, and fluorescence optical density were quantified. Lanthanum nitrate was traced by transmission electron microscopy. Results. After diagnostic ultrasound mediated microbubbles destruction, Evans Blue extravasation and fluorescence integrated optical density were significantly higher in the irradiated hemisphere than the contralateral side (all p < 0.01). Erythrocytes extravasations were demonstrated in the ultrasound-exposed hemisphere (4 1, grade 2) while being invisible in the control side. Lanthanum nitrate tracers leaked through interendothelial cleft and spread to the nerve fiber existed in the irradiation side. Conclusions. Transtemporal bone irradiation under DUS mediated microbubble destruction provides us with a more accessible, safer, and higher selective BBB opening approach in rats, which is advantageous in brain targeted drugs delivery.
PubMed | Fujian Provincial Hospital, University of Sichuan, Zhengzhou University and Sichuan Provincial Hospital for Women and Children
Type: Journal Article | Journal: Journal of clinical anesthesia | Year: 2015
To evaluate the effect of remifentanil on the isoflurane end-tidal concentration required to eliminate movement reaction upon surgical incision in children.Prospective, double blinded, serial study.Operating room of a university-affiliated hospital.Patients of ASA status 1 or 2, aged 4 to 7 years, scheduled for either inguinal hernia repair or orchidopexy surgery with general anesthesia.After endotracheal intubation, 108 children serially received 1 of 6 dose (nil, 0.05, 0.10, 0.15, 0.20, or 0.25 g kg(-1) min(-1)) of remifentanil. End-tidal isoflurane concentration was adjusted according to a Dixons up-and-down approach. Twenty-five minutes after starting the remifentanil infusion, the surgical incision was performed. The response of patients was classified as either response or no response. Response was defined as a purposeful response in response to skin incision.The MAC of isoflurane were 1.50 0.16%, 1.33 0.27%, 0.93 0.13%, 0.73 0.27%, 0.63 0.19%, and 0.60 0.15% for remifentanil infusion rates of nil, 0.05, 0.10, 0.15, 0.20, and 0.25 g kg(-1) min(-1), respectively.The MAC of isoflurane decreased with increasing infusion rate of remifentanil, showing an initial step reduction followed by a ceiling effect.
PubMed | Sichuan Provincial Hospital for Women and Children
Type: Journal Article | Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2014
To study the mortality of children under five and the causes of death together with related trend of dynamics, from 2001 to 2013 in Sichuan province.Using the Children Death Monitoring Network under five in Sichuan province to obtain basic data. Descriptive statistics and chi-square were used to describe the mortalities in children and infants as well as the causes of death, in both rural and urban areas of Sichuan province.In Sichuan province, the mortality of children under five decreased from 35.30 in 2001 to 11.77 in 2013. In 2013, mortality in the rural areas was 2.37 times more than that in the urban area. The proportion of neonatal deaths among the mortality in children under five was 44.72%. Pneumonia, congenital heart diseases and premature or low birth weigh were the top three causes of death for children under five. Among them, the top three causes of death for urban area were congenital heart disease, drowning, and premature or low birth weight/birth asphyxia. Meanwhile, the top three causes of death in rural areas were pneumonia, premature birth/low birth weight and birth asphyxia. Overall, the mortality rates of birth asphyxia, pneumonia and low birth weight gradually decreasd but drowning, diarrhea and traffic accidents fluctuated.The mortality of children under five in Sichuan province was 13, which had already met the goal set for the year 2020. However, reducing the mortality in rural areas, narrowing the gap between urban and rural areas seemed the main part of the future endeavor while focus of prevention should be adjusted according to the causes of death.