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Pu J.,Sichuan Provincial Hospital for Women and Children | Zeng W.-Y.,University of Sichuan
Journal of Sichuan University (Medical Science Edition) | Year: 2010

Objective To investigate the relationship between tumor necrosis factor receptor II (TNFR II ) gene 196T/G polymorphism and preterm labor in Han population in Chengdu. Methods Samples were collected from 96 subjects in corresponding period, 46 preterm labor pregnant women (we collected partial placental tissues of preterm labor, 21 cases of infectious group with chorioamnionitis, 25 cases of noninfectious group without chorioamnionitis), and 50 normal labor pregnant women. The DNA was extracted from each sample by using Chelex-100 method, then PCR-RFLP was performed to determine the TNFR II 196 gene polymorphism. Results 1 TNFR II 196 genotype frequencies of 196M/M(TT), 196M/R(TG) + 196R/R(GG) were 71. 7%, 21. 7% + 6.5% and 80.0%, 20.0%+0.0% in preterm labor and normal control group respectively. Allele frequencies of R (G), M (T) were 17.4%, 82.6% and 10.0%, 90.0%, respectively. There were no significant difference in frequencies of genotype and allele in TNFR II 196 gene polymorphism between two groups (P>0. 05, P>0. 05, respectively). 2 Close correlation was observed between the different genotypes and the chorioamnionitis (x2 = 11.088,P<0. 05). The odds ratio (OP) for TG+GG genotype was 12.65, 95% CI 2.359, 67.848, with more than 12. 65 times probability of chorioamnionitis than that of TT genotype group. Conclusion Polymorphism in 196 site of TNFR II gene was not crucial in preterm labor genesis, TG(GG) genotype may contribute to susceptibility to chorioamnionitis in the process of preterm labor in Chinese Han population. Source


Zhou S.,University of Sichuan | Yu P.,West Health Institute | Yu P.,University of Sichuan | Guan L.,West Health Institute | And 5 more authors.
European Journal of Obstetrics Gynecology and Reproductive Biology | Year: 2013

Objectives: The underlying mechanisms of protective immunity of placental trophoblast cells against bacterial infection remain largely unknown. NOD1 are intracellular pattern recognition receptors that are activated by bacterial peptides and mediate innate immunity. This study aimed to investigate the expression and function of NOD1 in first trimester trophoblast cells, and evaluate the potential role of trophoblast cells in infection-associated inflammation. Study design: Human extravillous trophoblast cell line HTR8 cells were stimulated with various concentrations of iE-DAP for various periods of time. NOD1 expression was detected by immunofluorescence, and the changes in NOD1 and RICK mRNA and protein in H8 cells were determined by real-time polymerase chain reaction and Western blot analysis. The concentrations of interleukin (IL)-8 and IL-6 secreted by H8 cells were examined by enzyme-linked immunosorbent assay. NF-κB transcription activity and P65 expression were detected by electrophoretic mobility shift assay and Western blot analysis. Results: H8 cells expressed NOD1, and the effects of iE-DAP on NOD1 were dose- and time-dependent. The concentration of IL-8 increased gradually with increasing concentration of iE-DAP, and the levels of IL-8 and IL-6 were associated with the duration of exposure to iE-DAP. The dose of iE-DAP was significantly associated with expression of RICK and P65, and stimulation of H8 cells by iE-DAP altered NF-κB transcription activity. Conclusions: NOD1 may have a role in mediating infection-associated inflammation. Once iE-DAP is recognized by NOD1, the inflammatory response may be induced via NOD1-RICK-NF-κB-mediated pathways. © 2013 Elsevier Ireland Ltd. All rights reserved. Source


Chen B.-Z.,University of Sichuan | Luo L.-H.,Zhejiang University | Jiang L.,Sichuan Provincial Peoples Hospital | Wang R.-R.,University of Sichuan | And 2 more authors.
Journal of Clinical Anesthesia | Year: 2011

Study Objective: To investigate intracuff pressure changes in the ProSeal Laryngeal Mask Airway (PLMA) during 50% nitrous oxide (N2O) anesthesia. Design: Prospective, randomized study. Setting: Operating room of a university-affiliated hospital. Patients: 40 ASA 1 and 2 children, aged 2 to 6 years, weighing 10-20 kg, undergoing elective inguinal herniorrhaphy with general anesthesia. Interventions and Measurements: Patients were assigned to two groups (n = 20). Patients in Group A were anesthetized with 50% N 2O in oxygen and sevoflurane, while patients in Group B were anesthetized with 50% air in oxygen and sevoflurane. PLMA cuffs were inflated with air. PLMA intracuff pressures starting from a baseline pressure of 30 mmHg were recorded using a pressure transducer for 30 minutes. The occurrence of postoperative throat discomfort was recorded. Main Results: In Group A, who inhaled 50% N2O for 30 minutes, intracuff pressures reached 86.7 ± 10.4 mmHg, while in Group B, who inhaled 50% air for 30 minutes, intracuff pressures were 29.7 ± 1.0 mmHg. The postoperative throat complication rate in Group A was 45%, which was significantly higher than in Group B (5%). Conclusion: PLMA intracuff pressure increased significantly during 50% N2O anesthesia. The children's postoperative throat complication rate also was increased. © 2011 Elsevier Inc. Source


Li S.-M.,Sichuan Provincial Hospital for Women and Children
Chinese Journal of Evidence-Based Medicine | Year: 2010

Objective: To investigate the application of hysteroscopy in pathological changes of infertility uterus. Methods: The clinical data of 226 cases of infertility females receiving hysteroscopy from January 2007 to June 2009 in Sichuan Provincial Hospital for Women and Children were retrospectively analyzed. Results: In 226 cases, hysteroscopic examination identified 147 cases of intrauterine diseases (65.04%), including 56 cases of intrauterine adhesion (24.78%), 32 cases of endometritis (14.16%), 27 cases of endometrial polyps (11.94%), 15 cases of uterine malformation (6.64%), 9 cases of submucous myoma (3.98%), 3 cases of endometrial tuberculosis (1.33%), 3 cases of uterus cavity narrow (1.33%), 2 cases of cervical internal relaxation (0.88%). No postoperative complications occurred, except for a small amount of vaginal bleeding. Conclusion: For the diagnosis of the pathological changes in uterus, hysteroscopy is a direct and accurate method with less operative duration, less trauma, less pain, quick recovery, no complications, and no necessity for hospitalization. It is worth to be popularized. © 2010 Editorial Board of Chin J Evid-based Med. Source


Dai L.,University of Sichuan | Wang W.,University of Sichuan | Zhang S.,University of Sichuan | Jiang Q.,Sichuan Provincial Hospital for Women and Children | And 5 more authors.
Cell Biology International | Year: 2012

miR-15 (microRNA 15) and miR-16 are frequently deleted or down-regulated in many cancer cell lines and various tumour tissues, suggesting that miR-15a/16-1 plays important roles in tumour progression and might be a method for cancer treatment. We have developed a vector-based plasmid to explore the anti-tumour efficacy of miR-15a/16-1 in colon cancer in vivo. It is proposed that miR-15a and miR-16-1 target cyclin B1 (CCNB1), which associates with several tumorigenic features such as survival and proliferation. The levels of miR-15a and miR-16-1 in colon cancer cells were inversely correlated with CCNB1 expression, and there was consensus between miR-15a/16-1 and CCNB1 mRNA sequences by analysing homology. Vector-based miR-15a/16-1 expression plasmid was constructed and transfected into HCT 116 and SW620 colon cancer cells in vitro. The effects produced on cell viability and angiogenesis were analysed using flow cytometric analysis, colony formation analysis and tube formation analysis. CCNB1 expression down-regulation was checked by Western blotting. Systemic delivery of miR-15a/16-1 plasmids encapsulated in cationic liposome led to a significant inhibition of subcutaneous tumour growth and angiogenesis in tumour tissues, whereas no effects were observed with liposome carrying the non-specific plasmid. In summary, miR-15a/16-1 has been applied in colon cancer treatment in vivo, and resulted in effective colon tumour xenografts growth arrest and angiogenesis decrease. These findings suggest that systemic delivery of vector-based miR-15a/16-1 expression plasmid can be an approach to colon cancer therapy. © The Author(s) Journal Compilation © 2012 International Federation for Cell Biology. Source

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