Sichuan Provincial Hospital for Women and Children

Chengdu, China

Sichuan Provincial Hospital for Women and Children

Chengdu, China
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Liao C.-K.,Sichuan Provincial Hospital for Women and Children | Liu J.-T.,Sichuan Provincial Hospital for Women and Children | Kang M.,Sichuan Provincial Hospital for Women and Children
World Chinese Journal of Digestology | Year: 2017

AIM To assess the value of X-ray barium meal contrast examination in the diagnosis of constipationpredominant irritable bowel syndrome (C-IBS). METHODS From October 2014 to September 2016, 574 women with constipation visited our hospital, of which 165 with C-IBS and 80 with functional constipation (FC) were included in this study and underwent X-ray barium meal contrast examination. According to the structure, morphology and functional changes of the colon as revealed by X-ray barium meal contrast examination, clinical symptoms such as abdominal pain, abdominal discomfort, constipation, and difficulty in defecation as well as disease severity were assessed. RESULTS The transverse colon of patients in both groups exhibited structural or morphological changes such as γ, ω and m types, intestinal cavity expansion, redundant or tortuous sigmoid colon. The anatomical location of abdominal pain was consistent with structural or morphological changes in the colon of C-IBS patients. The redundant or tortuous sigmoid colon in both groups was significantly associated with hard stools, reduced defecation frequency, and difficulty in defecation described in the Bristol Stool Form Scale (P > 0.05). Slow colonic peristalsis, colon rigidity, intestinal cavity expansion and colonic wall changes were significantly associated with C-IBS abdominal pain grade and bowel abnormalities (P < 0.05). Colonic wall changes often suggested slow colonic peristalsis, colonic rigidity, decreased colonic flexibility and intestinal cavity expansion. FC patients lacked typical intestinal irritable symptoms. CONCLUSION X-ray barium contrast examination is of high value in evaluating colorectal morphology, structure and dysfunction and can be used for diagnosing IBS-C. © The Author(s) 2017.

Li W.,University of Sichuan | Han J.,Sichuan Provincial Hospital for Women and Children | Wu Z.-P.,Jiujiang No1 Peoples Hospital | Wu H.,University of Sichuan
World Journal of Gastroenterology | Year: 2017

Aim: to investigate the short-term outcomes and risk factors indicating postoperative death of patients with lesions adjacent to the hepatocaval confluence. Methods: We retrospectively analyzed 54 consecutive patients who underwent hepatectomy combined with inferior vena cava (IVC) and/or hepatic vein reconstruction (HVR) from January 2012 to January 2016 at our liver surgery center. The patients were divided into 5 groups according to the range of IVC and hepatic vein involvement. The patient details, indications for surgery, operative techniques, intra- and postoperative outcomes were compared among the 5 groups. Univariate and multivariate analyses were performed to explore factors predictive of overall operative death. Results: IVC replacement was carried out in 37 (68.5%) patients and HVR in 17 (31.5%) patients. Type I2H2 had the longest operative blood loss, operative duration and overall liver ischemic time (all, P < 0.05). Three patients of Type I3H1 with totally occluded IVC did not need IVC reconstruction. Total postoperative morbidity rate was 40.7% (22 patients) and the operative mortality rate was 16.7 % (9 patients). Factors predictive of operative death included IVC replacement (P = 0.048), duration of liver ischemia (P = 0.005) and preoperative liver function being Child-Pugh B (P = 0.025). Conclusion: IVC replacement, duration of liver ischemia and preoperative poor liver function were risk factors predictive of postoperative death. We should be cautious about IVC replacement, especially in Type I2H2. For Type I3H1, it was unnecessary to replace IVC when the collateral circulation was established. © 2017 Baishideng Publishing Group Inc. All rights reserved.

Li L.,Lanzhou University | Ding J.,Lanzhou University | Han J.,Sichuan Provincial Hospital for Women and Children | Wu H.,University of Sichuan
Medicine (United States) | Year: 2017

Surgical site infection (SSI) is one of the major morbidities after radical resection for perihilar cholangiocarcinoma (PHCC). This study aimed to clarify the risk factors and construct a nomogram to predict SSIs in patients with PHCC. A total of 335 consecutive patients who underwent hepatectomy combined with hepaticojejunostomy between January 2013 and December 2015 were analyzed retrospectively. SSIs, including incisional (superficial and deep) and space/organ infection, were defined according to the Centers for Disease Control and Prevention (CDC)'s National Nosocomial Infection Surveillance (NNIS) system. Risk factors associated with postoperative SSIs were analyzed by univariate and multivariate analyses. A nomogram was developed on the basis of results from the multivariate logistic model and the discriminatory ability of the model was analyzed. PHCC patients had higher organ/space SSI rate than incisional SSI rate after radical resection. Multivariate analysis showed that risk factors indicating postoperative overall SSIs (incisional and organ/space) included coexisting cholangiolithiasis [odds ratio (OR): 6.77; 95% confidence interval (95% CI): 2.40-19.11; P<.001], blood loss >1500mL (OR: 4.77; 95% CI: 1.45-15.65; P=.010), having abdominal surgical history (OR: 5.85; 95% CI: 1.91-17.97; P=.002), and bile leakage (OR: 15.28; 95% CI: 5.90-39.62; P<.001). The β coefficients from the multivariate logistic model were used to construct the model for estimation of SSI risk. The scoring model was as follows: -4.12 +1.91 × (coexisting cholangiolithiasis=1) + 1.77 × (having previous abdominal surgical history=1) +1.56 × (blood loss >1500mL=1) + 2.73 × (bile leakage=1). The discriminatory ability of the model was good and the area under the receiver operating characteristic (ROC) curve (AUC) was 0.851. In PHCC patients, there may be a relationship between postoperative SSIs and abdominal surgical history, coexisting cholangiolithiasis, bile leakage, and blood loss. The nomogram can be used to estimate the risk of postoperative SSIs in patients with PHCC. © 2017 the Author(s).

Yuan Z.-X.,Chinese Academy of Sciences | Wu X.-J.,Integrative Traditional and Western Medicine Hospital of Sichuan Province | Mo J.,University of Western Australia | Wang Y.-L.,Sichuan Provincial Hospital for Women and Children | And 2 more authors.
European Journal of Pharmaceutics and Biopharmaceutics | Year: 2015

We have previously demonstrated that peptide fragments (PFs) of the human serum albumin could be developed as potential renal targeting carriers, in particular, the peptide fragment, PF-A299-585 (A299-585 representing the amino acid sequence of the human serum albumin). In this paper, we conjugated triptolide (TP), the anti-inflammatory Chinese traditional medicine, to PF-A299-585 via a succinic acid spacer to give TPS-PF-A299-585 (TP loading 2.2% w/w). Compared with the free TP, TPS-PF-A299-585 exhibited comparable anti-inflammatory activity in the lipopolysaccharide stimulated MDCK cells, but was significantly less cytotoxic than the free drug. Accumulation of TPS-PF-A299-585 in the MDCK cells in vitro and in rodent kidneys in vivo was demonstrated using FITC-labeled TPS-PF-A299-585. Renal targeting was confirmed in vivo in a membranous nephropathic (MN) rodent model, where optical imaging and analyses of biochemical markers were combined to show that TPS-PF-A299-585 was capable of alleviating the characteristic symptoms of MN. The collective data affirm PF-A299-585 to be a useful carrier for targeting TP to the kidney. © 2015 Elsevier B.V. All rights reserved.

PubMed | University of Sichuan, Peking Union Medical College and Sichuan Provincial Hospital For Women and Children
Type: Journal Article | Journal: Stem cell research & therapy | Year: 2016

Liver disease is a major cause of death worldwide. Orthotropic liver transplantation (OLT) represents the only effective treatment for patients with liver failure, but the increasing demand for organs is unfortunately so great that its application is limited. Hepatocyte transplantation is a promising alternative to OLT for the treatment of some liver-based metabolic disorders or acute liver failure. Unfortunately, the lack of donor livers also makes it difficult to obtain enough viable hepatocytes for hepatocyte-based therapies. Currently, a fundamental solution to this key problem is still lacking. Here we show a novel non-transgenic protocol that facilitates the rapid generation of functional induced hepatocytes (iHeps) from human adipose-derived stem cells (hADSCs), providing a source of available cells for autologous hepatocytes to treat liver disease.We used collagenase digestion to isolate hADSCs. The surface marker was detected by flow cytometry. The multipotential differentiation potency was detected by induction into adipocytes, osteocytes, and chondrocytes. Passage 3-7 hADSCs were induced into iHeps using an induction culture system composed of small molecule compounds and cell factors.Primary cultured hADSCs presented a fusiform or polygon appearance that became fibroblast-like after passage 3. More than 95 % of the cells expressed the mesenchymal cell markers CD29, CD44, CD166, CD105, and CD90. hADSCs possessed multipotential differentiation towards adipocytes, osteocytes, and chondrocytes. We rapidly induced hADSCs into iHeps within 10 days in vitro; the cellular morphology changed from fusiform to close-connected cubiform, which was similar to hepatocytes. After induction, most of the iHeps co-expressed albumin and alpha-1 antitrypsin; they also expressed mature hepatocyte special genes and achieved the basic functions of hepatocyte. Moreover, iHep transplantation could improve the liver function of acute liver-injured NPG mice and prolong life.We isolated highly purified hADSCs and rapidly induced them into functional hepatocyte-like cells within 10 days. These results provide a source of available cells for autologous hepatocytes to treat liver disease.

Chen B.-Z.,Sichuan Provincial Hospital for Women and Children | Tan L.,University of Sichuan | Zhang L.,Sichuan Orthopaedic Hospital | Shang Y.-C.,University of Sichuan
Journal of Clinical Anesthesia | Year: 2013

Study Objective: To test the hypothesis that muscle relaxant is not necessary in patients who are undergoing laparoscopic gynecological surgery with a ProSeal Laryngeal Mask Airway (ProSeal LMA™). Design: Prospective, randomized study. Setting: Operating room of Sichuan Provincial Hospital for Women and Children. Patients: 120 adult, ASA physical status 1 and 2 women, aged 18 to 55 years. Interventions and Measurements: Patients were randomly assigned to two groups (n=60) to receive a muscle relaxant (Group MR) or not (Group NMR). General anesthesia was used in patients of both groups for airway management with the ProSeal LMA. Peak airway inflation pressures, airway sealing pressure, minimum flow rate, and recovery time were assessed. Surgical conditions were assessed by the operating gynecologist. The frequency of sore throats was recorded. Main Results: ProSeal LMA insertion was 100% successful. Patients' lungs were ventilated with a maximum sealing pressure of 32 ± 5.1 cm H2O (Group MR) or 31 ± 4.9 cm H2O (Group NMR) (P = 0.341). The seal quality in both groups permitted the use of low flows: 485 ± 291 mL/min in Group MR and 539 ± 344 mL/min in Group NMR (P = 0.2). Surgical conditions were comparable between the two groups. There was no difference in the frequency of sore throats (20% vs 21.7%; P = 0.28). In Group NMR, there was a statistically significant reduction in recovery time versus Group MR (4.5 ± 2.6 min vs 10.3 ± 4.2 min; P < 0.01). Conclusion: Muscle relaxant is not necessary in general anesthesia with a ProSeal LMA. © 2013 Elsevier Inc. All Rights Reserved.

Zhuang L.,Sichuan Provincial Hospital for Women and Children | Chen D.-X.,Sichuan Provincial Hospital for Women and Children | Yao Y.-H.,Sichuan Provincial Hospital for Women and Children
Journal of Reproduction and Contraception | Year: 2013

Cesarean scar pregnancy (CSP) occurs when a gestation sac is implanted in the previous lower segment cesarean scar. The incidence of CSP is increasing worldwide. Uterine ateriovenous malformation (UAVM) is a rare gynecologic disease. Both of these diseases can cause severe vaginal bleeding and produce high morbidity rate. We describe a case of UAVM induced by a CSP. The patient suffered intermittent vaginal bleeding after two dilatation and curettage (D&C) one month before admission. The suspected diagnosis of CSP and UAVM were made after using transvaginal ultrasound with color Doppler and human chorionic gonadotropin (hCG) examination. Resection of the involved area including the cesarean scar and UAVM by laparotomy was performed successfully and the diagnosis was confirmed by the last pathologic result. © 2013 The Editorial Board of Journal of Reproduction and Contraception.

Li S.-M.,Sichuan Provincial Hospital for Women and Children
Chinese Journal of Evidence-Based Medicine | Year: 2010

Objective: To investigate the application of hysteroscopy in pathological changes of infertility uterus. Methods: The clinical data of 226 cases of infertility females receiving hysteroscopy from January 2007 to June 2009 in Sichuan Provincial Hospital for Women and Children were retrospectively analyzed. Results: In 226 cases, hysteroscopic examination identified 147 cases of intrauterine diseases (65.04%), including 56 cases of intrauterine adhesion (24.78%), 32 cases of endometritis (14.16%), 27 cases of endometrial polyps (11.94%), 15 cases of uterine malformation (6.64%), 9 cases of submucous myoma (3.98%), 3 cases of endometrial tuberculosis (1.33%), 3 cases of uterus cavity narrow (1.33%), 2 cases of cervical internal relaxation (0.88%). No postoperative complications occurred, except for a small amount of vaginal bleeding. Conclusion: For the diagnosis of the pathological changes in uterus, hysteroscopy is a direct and accurate method with less operative duration, less trauma, less pain, quick recovery, no complications, and no necessity for hospitalization. It is worth to be popularized. © 2010 Editorial Board of Chin J Evid-based Med.

Wang L.-C.,University of Sichuan | Chen E.-Q.,University of Sichuan | Cao J.,Sichuan Provincial Hospital for Women and Children | Liu L.,University of Sichuan | And 6 more authors.
Hepatology International | Year: 2011

Purpose: Either combination treatment or monotherapy using agents with a high genetic barrier are recommended for hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB). The aim of this study was to compare effect of naïve HBeAg-negative CHB patients with either de novo combination of lamivudine (LAM) and adefovir dipivoxil (ADV) or entecavir (ETV) monotherapy. Methods: HBeAg-negative CHB patients (n = 71) with ALT levels between 2 and 10 times the upper normal limit and HBV DNA levels >104 copies/mL were enrolled. Patients were treated with either LAM 100 mg plus ADV 10 mg per day (n = 31) or ETV 0.5 mg per day (n = 40) for 48 weeks. Results: The average reduction in HBV DNA level compared with baseline were 5.16 ± 1.69 log in the LAM + ADV group and 5.36 ± 1.70 log in the ETV group by week 48 (P = 0.624). The virological response (VR) rates were 80.65 and 77.5%, the biochemical response (BR) rates were 93.55 and 90.00% at week 48 in the LAM + ADV and ETV groups, respectively. There was no significant difference in the VR and BR between the two groups. During the 48-week treatment period, virological breakthrough and serious side effects were not noted in any patient. Conclusions: Both LAM + ADV combination therapy and ETV monotherapy are effective in naïve HBeAg-negative CHB patients, but further studies are needed to obtain long-term results. © 2011 Asian Pacific Association for the Study of the Liver.

PubMed | Chongqing Medical University and Sichuan Provincial Hospital for Women and Children
Type: | Journal: BioMed research international | Year: 2016

Objective. Blood-brain barrier (BBB) is a key obstacle that prevents the medication from blood to the brain. Microbubble-enhanced cavitation by focused ultrasound can open the BBB and proves to be valuable in the brain drug delivery. The study aimed to explore the feasibility, efficacy, and safety of unilateral opening of BBB using diagnostic ultrasound targeted microbubbles destruction in rats. Methods. A transtemporal bone irradiation of diagnostic ultrasound and intravenous injection of lipid-coated microbubbles were performed at unilateral hemisphere. Pathological changes were monitored. Evans Blue extravasation grades, extraction from brain tissue, and fluorescence optical density were quantified. Lanthanum nitrate was traced by transmission electron microscopy. Results. After diagnostic ultrasound mediated microbubbles destruction, Evans Blue extravasation and fluorescence integrated optical density were significantly higher in the irradiated hemisphere than the contralateral side (all p < 0.01). Erythrocytes extravasations were demonstrated in the ultrasound-exposed hemisphere (4 1, grade 2) while being invisible in the control side. Lanthanum nitrate tracers leaked through interendothelial cleft and spread to the nerve fiber existed in the irradiation side. Conclusions. Transtemporal bone irradiation under DUS mediated microbubble destruction provides us with a more accessible, safer, and higher selective BBB opening approach in rats, which is advantageous in brain targeted drugs delivery.

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