Sichuan Provincial Orthopedic Hospital

Chengdu, China

Sichuan Provincial Orthopedic Hospital

Chengdu, China
SEARCH FILTERS
Time filter
Source Type

Zhong R.-X.,Sichuan Provincial Orthopedic Hospital | Feng G.-F.,Sichuan Provincial Orthopedic Hospital | Liu Y.-J.,Chengdu University of Traditional Chinese Medicine | Zhong L.,Chengdu University of Traditional Chinese Medicine | And 2 more authors.
Tropical Journal of Pharmaceutical Research | Year: 2016

Purpose: To develop a high-performance liquid chromatography (HPLC) fingerprint method for the quality control and origin discrimination of Gastrodiae rhizoma. Methods: Twelve batches of G. rhizoma collected from Sichuan, Guizhou and Shanxi provinces in china were used to establish the fingerprint. The chromatographic peak (gastrodin) was taken as the reference peak, and all sample separation was performed on a Agilent C18 (250 mm×4.6 mmx5 μm) column with a column temperature of 25 °C. The mobile phase was acetonitrile/0.8 % phosphate water solution (in a gradient elution mode) and the flow rate of 1 mL/min. The detection wavelength was 270 nm. The method was validated as per the guidelines of Chinese Pharmacopoeia. Results: The chromatograms of the samples showed 11 common peaks, of which no. 4 was identified as that of Gastrodin. Data for the samples were analyzed statistically using similarity analysis and hierarchical cluster analysis (HCA). The similarity index between reference chromatogram and samples’ chromatograms were all > 0.80. The similarity index of G. rhizoma from Guizhou, Shanxi and Sichuan is evident as follows: 0.854 - 0.885, 0.915 - 0.930 and 0.820 - 0.848, respectively. The samples could be divided into three clusters at a rescaled distance of 7.5: S1 - S4 as cluster 1; S5 - S8 cluster 2, and others grouped into cluster 3. Conclusion: The findings indicate that HPLC fingerprinting technology is appropriate for quality control and origin discrimination of G. rhizoma. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.


Zhu L.-Y.,Sichuan Provincial Orthopedic Hospital | Ma J.,Sichuan Provincial Orthopedic Hospital | Tu H.,Sichuan Provincial Orthopedic Hospital
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: To compare the release and transdermal behavior in vitro between Dinggui Huoluo Rubber Patch (DHRP) and Dinggui Gel Paste (DGP) which is the improved form. Methods: Microporous filter membrane or Strat-M membrane and advanced Franz diffusion cell were chosen in the release and transdermal experiment in vitro. The content of notopterol, the active component of the key herb Notopterygium incisum in the preparation, was quantitatively determined by HPLC method to determine the release rate, penetration quantity, and penetration rate of the two forms. Results: The penetration formula of DGP was Ln = 9.208 t-18.50, r = 0.948. The in vitro cumulative release rate, penetration quantity, and penetration rate were respectively 85.34%, 0.661 8 mg/g, and 65.67%. The penetration formula of DHRP was Ln = 4.285 t-6.704, r = 0.977. The in vitro cumulative release rate, penetration quantity, and penetration rate were respectively 67.87%, 0.181 6 mg/g, and 31.35%. Conclusion: The transdermal behavior of DGP is obviously better than that of DHRP while its quantity and the rate of penetration and release are all better than those of rubber patch. Meanwhile the gel paste is less sensitized and more convenient to use. © 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Loading Sichuan Provincial Orthopedic Hospital collaborators
Loading Sichuan Provincial Orthopedic Hospital collaborators