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Wang Y.C.,Sichuan Agricultural University | Deng J.L.,Sichuan Agricultural University | Xu S.W.,Northeast Agricultural University | Peng X.,Sichuan Key Laboratory of Animal Disease and Human Health | And 4 more authors.
Poultry Science | Year: 2012

Zearalenone (ZEA) is an estrogenic mycotoxin. It is produced by several Fusarium species and can contaminate food and feed. To investigate the role of calcium homeostasis in ZEA-induced toxicity of poultry and elucidate its cytotoxic mechanism, splenic lymphocytes isolated from chickens were exposed to ZEA (0-25 μg/mL) for 48 h. The intracellular calcium concentration ([Ca2+]i), pH, calmodulin (CaM) mRNA levels, and Na+/K+-ATPase activities and Ca2+-ATPase activities were detected by the fluorescent dyes Fluo-3/AM and BCECF/AM, quantitative real-time PCR, and chromatometry. Supernatant CaM concentrations were simultaneously detected by ELISA. As the ZEA exposure concentration increased, the [Ca2+] i and CaM mRNA levels gradually increased, while intracellular pH, CaM concentrations of supernatants, and intracellular Na+/K+-ATPase and Ca2+-ATPase activities gradually decreased in a dose-dependent manner. There were significant differences (P < 0.05 or P < 0.01) between the treatment groups and the control group. These results indicate that ZEA cytotoxicity arises by causing an imbalance in calcium homeostasis and intracellular acidification in lymphocytes. © 2012 Poultry Science Association Inc.


Wang Y.C.,Sichuan Agricultural University | Deng J.L.,Sichuan Agricultural University | Xu S.W.,Northeast Agricultural University | Peng X.,Sichuan Key Laboratory of Animal Disease and Human Health | And 4 more authors.
The Scientific World Journal | Year: 2012

Zearalenone (ZEN) is an estrogenic mycotoxin produced by several Fusarium species, which can contaminate food and feed. These compounds elicit a wide spectrum of toxic effects, including the capacity to alter normal immune function. In this study, the in vitro effects of the treatment of ConA-stimulated splenic lymphocytes with ZEN (0-25g/mL) were examined. ZEN modulates the expression of IL-2, IL-6, and IFN-. The IL-2 levels were up to fourfold higher (P<0.05) compared with the levels in the control at toxin concentrations of 25g/mL after 48h of treatment. The IL-6 levels were critically suppressed at this concentration; these changes were very statistically significant (P<0.05). At lower ZEN concentrations (0.1, 0.4 and 1.6g/mL), the IFN- levels changed slightly; however at 6.25 and 25g/mL, the IFN- results reached statistical significance compared with the control levels (P<0.05). These data suggest that ZEN has potent effects on the expression of chicken splenic lymphocytes cytokines at the mRNA level. Copyright © 2012 Y. C. Wang et al.

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