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Wang P.,Kunming University of Science and Technology | Xiao Y.,Kunming Nanjiang Pharmaceutical Corporation of Ltd | Liu W.,Sichuan Kelun Pharmaceutical Co. | Wang J.,Kunming University of Science and Technology | Yang Y.,Kunming University of Science and Technology
Food Chemistry | Year: 2014

A rapid, simple, sensitive and environmentally friendly method has been developed for the determination of three oestrogens (17β-estradiol (17β-E2), estrone (E1), and diethylstilbestrol (DES)) in milk samples by using vortex-assisted hollow fibre liquid-phase microextraction (VA-HF-LPME) and high performance liquid chromatography. Method is based on the microextraction of oestrogens from sample solution into 15 μL of nonanoic acid as extracting agent, which is placed inside the hollow fibre followed by vortex-mixing. Vortex provided effective and mild mixing of sample solution and increased the contact between analytes and boundary layers of the hollow fibre, thereby enhancing mass transfer rate and leading to high recovery of target analytes. The extraction equilibrium is achieved within 2 min. Parameters influencing the recovery were investigated and optimized. The proposed technique provided good linearity (>0.9984), repeatability (RSD = 2.56-4.38), low limits of detection (0.06-0.17 ng mL-1), and high enrichment factor (330). © 2014 Elsevier Ltd. All rights reserved.

Zhang Z.-W.,Sichuan Agricultural University | Xu X.-C.,Chengdu University of Technology | Liu T.,Sichuan Kelun Pharmaceutical Co. | Yuan S.,Sichuan Agricultural University
Oxidative Medicine and Cellular Longevity | Year: 2016

Reactive oxygen species (ROS) play a crucial role in the inflammatory response and cytokine outbreak, such as during virus infections, diabetes, cancer, cardiovascular diseases, and neurodegenerative diseases. Therefore, antioxidant is an important medicine to ROS-related diseases. For example, ascorbic acid (vitamin C, VC) was suggested as the candidate antioxidant to treat multiple diseases. However, long-term use of high-dose VC causes many side effects. In this review, we compare and analyze all kinds of mitochondrion-permeable antioxidants, including edaravone, idebenone, α-Lipoic acid, carotenoids, vitamin E, and coenzyme Q10, and mitochondria-targeted antioxidants MitoQ and SkQ and propose astaxanthin (a special carotenoid) to be the best antioxidant for ROS-burst-mediated acute diseases, like avian influenza infection and ischemia-reperfusion. Nevertheless, astaxanthins are so unstable that most of them are inactivated after oral administration. Therefore, astaxanthin injection is suggested hypothetically. The drawbacks of the antioxidants are also reviewed, which limit the use of antioxidants as coadjuvants in the treatment of ROS-associated disorders. © 2016 Zhong-Wei Zhang et al.

Yang Q.,Shenyang Pharmaceutical University | Ma Y.,Sichuan Kelun Pharmaceutical Co. | Zhao Y.,Shenyang Pharmaceutical University | She Z.,Shenyang Pharmaceutical University | And 4 more authors.
International Journal of Nanomedicine | Year: 2013

Background: Sequential low-dose chemotherapy has received great attention for its unique advantages in attenuating multidrug resistance of tumor cells. Nevertheless, it runs the risk of producing new problems associated with the accelerated blood clearance phenomenon, especially with multiple injections of PEGylated liposomes. Methods: Liposomes were labeled with fluorescent phospholipids of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and epirubicin (EPI). The pharmacokinetics profile and biodistribution of the drug and liposome carrier following multiple injections were determined. Meanwhile, the antitumor effect of sequential low-dose chemotherapy was tested. To clarify this unexpected phenomenon, the production of polyethylene glycol (PEG)-specific immunoglobulin M (IgM), drug release, and residual complement activity experiments were conducted in serum. Results: The first or sequential injections of PEGylated liposomes within a certain dose range induced the rapid clearance of subsequently injected PEGylated liposomal EPI. Of note, the clearance of EPI was two- to three-fold faster than the liposome itself, and a large amount of EPI was released from liposomes in the first 30 minutes in a complement-activation, direct-dependent manner. The therapeutic efficacy of liposomal EPI following 10 days of sequential injections in S180 tumor-bearing mice of 0.75 mg EPI/kg body weight was almost completely abolished between the sixth and tenth day of the sequential injections, even although the subsequently injected doses were doubled. The level of PEG-specific IgM in the blood increased rapidly, with a larger amount of complement being activated while the concentration of EPI in blood and tumor tissue was significantly reduced. Conclusion: Our investigation implied that the accelerated blood clearance phenomenon and its accompanying rapid leakage and clearance of drug following sequential low-dose injections may reverse the unique pharmacokinetic-toxicity profile of liposomes which deserved our attention. Therefore, a more reasonable treatment regime should be selected to lessen or even eliminate this phenomenon. © 2013 Yang et al, publisher and licensee Dove Medical Press Ltd.

Sichuan Kelun Pharmaceutical Co. | Date: 2008-10-28


Sichuan Kelun Pharmaceutical Co. | Date: 2015-11-30

Needles for medical purposes; suture needles; catheters; Bed pans; Incontinence bed pads; Hospital beds for use by burn patients; cannulae; appliances for washing body cavities; Droppers for administering medication, sold empty; Syringes for medical purposes and for injections; drainage tubes for medical purposes; Dropper bottles for administering medication, sold empty; hypodermic syringes; Inhalers for medical purposes, sold empty; Medical fluid injectors; Insufflators for medical purposes; Medical apparatus and instruments for use in surgery; Dialysis machines; Cases fitted for medical instruments and/or for use by doctors; sphygmomanometers; stents; X-ray tubes for medical purposes.

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