Li B.,Chengdu University of Technology |
Li B.,Sichuan Huada Judical Expertize Institute |
Wan J.,Chengdu University of Technology |
Xu G.-Y.,Sichuan Huada Judical Expertize Institute |
And 4 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2012
Objective To investigate the effect of compressive stress on the factors for liver regeneration including NF-κB , signal transducers and activators of transcription 3 (STAT3), c-fos and c-jun in hepatocytes Chang cell line. Methods Human hepatocytes Chang cell line were subjected to compressive stress at 1000 μstain or 2000 μstain, expression of NF-κb P65, p-STAT3, c-fos and c-jun were detected by Western blot or RT-PCR at 30 min, l h , 2 h , 3 h , 4 h , 6h after application of compressive stress to indicate the priming of hepatocytes proliferation. Results Enhanced expressions of NF-κb P65 and p-STAT3 were observed in hepatocytes under compressive stress indicated by Western blot, the magnitude of compressive stress loaded significantly affected the level of expression of NF-κb P65 at 2 h (P = 0. 046) and p-STAT3 at 1 h (P = 0. 039) , the peak of expression of p-STAT3 was at 30 minutes after stress-loading while NF-κb P65 was at 1 hour; RT-PCR showed that expression of c-fos was not significantly different between 1000 μstain and 2000 μstain at each time point, and expression of c-jun was significantly different at 30 minutes (P = 0. 026), 1 h (P = 0. 031), 2 h (P = 0. 033) after compressive stress loading. Conclusion These results indicate that compressive stress may play an important role in initiating the process of liver regeneration.