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Wan Q.,Chengdu University of Traditional Chinese Medicine | Wan Q.,Sichuan Family Planning Research Institute | Lu H.,Chengdu University of Traditional Chinese Medicine | Lu H.,Sichuan Family Planning Research Institute | And 6 more authors.
Human Cell | Year: 2014

This study investigated the effects of the compound oleanolic acid (OA) in inducing mouse embryonic stem cells (MESC) to differentiate towards germ cells (GC). MESC 1B10 was used as the model cell. 1B10 was cultured, and embryoid bodies (EBs) were produced from 1B10. The EBs were allowed to attach to the bottoms of culturing disks and grow. OA was added into the medium to induce the EBs to differentiate. Retinoic acid (RA) was used as the positive drug. After 72 h, total RNA was extracted, cDNA was synthesized, and real-time fluorescence quantitative PCR was performed to measure the transcriptional expression profiles of 11 reproduction-related genes affected by OA and RA, respectively. When the data were compared, it was found that OA up-regulated the transcriptional levels of Oct-4, GDF-9, Stra8, Mvh, ZP2, ZP3, Itga6, and TP2, and down-regulated transcriptional levels of SCP3, ZP1, and Itgb1; RA up-regulated the transcriptional levels of GDF-9, Stra8, Mvh, ZP2, ZP3, Itga6, Itgb1, and TP2, and down-regulated the transcriptional levels of Oct-4, SCP3, and ZP1. The data showed that OA and RA had similar effects in inducing differentiation of MESC towards GC. © 2013 Japan Human Cell Society and Springer Japan. Source


Wan Q.,Chengdu University of Traditional Chinese Medicine | Wan Q.,Sichuan Family Planning Research Institute | Lu H.,Sichuan Family Planning Research Institute | Lu H.,Chengdu University of Traditional Chinese Medicine | And 7 more authors.
Human Cell | Year: 2014

The women during the menopause period have an increased tendency for the obesity, which represents the more fat production than during the premenopausal period. Although this is not beneficial overall, it could provide a compensatory source for the estrogen production for the menopausal women. So it would be meaningful to find an agent that could inhibit the fat production while does not disturb the total estrogen production by fat tissues. In the present study, the effect of oleanolic acid (OA) on the fat production and the total estrogen production of the differentiating mouse preadipocyte 3T3-L1 as well as the mechanisms behind those effects were preliminarily investigated. The cell line 3T3-L1 was chosen as the model cell because it is usually used for the research about the obesity. During the induced differentiation of 3T3-L1 cells, cells were intervened continuously with OA. The fat production was determined with the oil red staining assay and the total estrogen production was measured with the ELISA assay. Finally, the expression patterns for important genes of the fat production and the estrogen production were studied, respectively with the real-time fluorescence quantitative PCR (qPCR). The results showed that for the differentiating 3T3-L1 cells, OA could significantly inhibit the fat production and did not disturb the total estrogen production significantly. In the mechanism studies, OA was found to significantly down-regulate ACC, the key gene for fat synthesis, which could explain the inhibitory effect of OA on the fat production; OA was also found to significantly up-regulate CYP11A1, CYP17, CYP19, the key genes for the estrogen synthesis and significantly down-regulate CYP1A1, the key gene for the estrogen decomposition, which preliminarily explained the lack of the effect of OA on the total estrogen production. In conclusion, OA was found able to inhibit the fat production while maintaining the total estrogen level and the mechanisms for the above findings were preliminarily clarified, which suggests that OA may be useful to treat the menopausal obesity. © 2014, Japan Human Cell Society and Springer Japan. Source

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