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Wang Y.,Sichuan Agricultural University | Qiu M.,Sichuan Animal Science Academy | Yang J.,Sichuan Agricultural University | Zhao X.,Sichuan Agricultural University | And 2 more authors.
Infection, Genetics and Evolution | Year: 2014

The major histocompatibility complex (MHC) among chickens has been well established as being associated with disease resistance and pathogens infection, but the genetic differences in MHC between chickens susceptible to certain infections and those chickens that remain uninfected have not been sufficiently determined. In this study, we sought the genetic basis that may underlie differences in susceptibility to infection among chickens by challenging four groups of broilers with Marek's disease virus (MDV). Over the course of the experiment, lesions began to appear between 21 and 35. days post challenge (dpc), and commercial broilers were not necessarily better than indigenous chickens in terms of disease resistance. The four groups showed neutral resistance to MDV infection validated by challenge results and evolutionary analysis of exons 2 and 3 of the MHC class I region. Several variable sites in exon 2 and exon 3 were exclusively appeared in infected chickens. Exon 3 was likely more crucial than exon 2 in disease resistance. Our observations offered a support for a potential association between promiscuous pathogens and conspicuous genetic diversity in the MHC class I region. © 2013 Elsevier B.V.

PubMed | Sichuan Animal Science Academy, IRTA - Institute of Agricultural-Alimentary Research and Technology, University of Arkansas, Southwest University and Sichuan Agricultural University
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016

Orchardgrass (Dactylis glomerata L.), is a well-known perennial forage species; however, rust diseases have caused a noticeable reduction in the quality and production of orchardgrass. In this study, genetic diversity was assessed and the marker-trait associations for rust were examined using 18 EST-SSR and 21 SCoT markers in 75 orchardgrass accessions. A high level of genetic diversity was detected in orchardgrass with an average genetic diversity index of 0.369. For the EST-SSR and SCoT markers, 164 and 289 total bands were obtained, of which 148 (90.24%) and 272 (94.12%) were polymorphic, respectively. Results from an AMOVA analysis showed that more genetic variance existed within populations (87.57%) than among populations (12.43%). Using a parameter marker index, the efficiencies of the EST-SSR and SCoT markers were compared to show that SCoTs have higher marker efficiency (8.07) than EST-SSRs (4.82). The results of a UPGMA cluster analysis and a STRUCTURE analysis were both correlated with the geographic distribution of the orchardgrass accessions. Linkage disequilibrium analysis revealed an average r of 0.1627 across all band pairs, indicating a high extent of linkage disequilibrium in the material. An association analysis between the rust trait and 410 bands from the EST-SSR and SCoT markers using TASSEL software revealed 20 band panels were associated with the rust trait in both 2011 and 2012. The 20 bands obtained from association analysis could be used in breeding programs for lineage selection to prevent great losses of orchardgrass caused by rust, and provide valuable information for further association mapping using this collection of orchardgrass.

PubMed | Sichuan Animal Science Academy and Sichuan Agricultural University
Type: Journal Article | Journal: Bioscience, biotechnology, and biochemistry | Year: 2016

Increasing evidence suggests that micro (mi)RNAs play important roles in various biological process. To evaluate the roles of miRNA in the porcine liver, we investigated the dynamic profiles of microRNAomes using liver tissue from pigs during the embryonic period (embryonic day 90), weaning stage (postnatal day 30), and adult stage (7years old). A total of 186 unique miRNAs were differentially expressed during liver development. We also identified that 17, 13, and 6 miRNAs were specifically abundant at embryonic day 90, postnatal day 30, and at 7years, respectively. Besides regulating basic cellular roles in development, miRNAs expressed at the three developmental stages also participated in regulating embryonic liver development, early hepatic growth and generating a functioning liver, and energy metabolic processes, respectively. Our study indicates that miRNAs are extensively involved in liver development, and provides a valuable resource for the further elucidation of miRNA regulatory roles during liver development.

PubMed | Sichuan University and Sichuan Animal Science Academy
Type: | Journal: Veterinary microbiology | Year: 2016

Previous reports showed that infection of porcine reproductive and respiratory syndrome virus (PRRSV) stimulated a variable host response and pig susceptibility to PRRSV was largely dependent on its genetic composition. In the present study, host susceptibility of Tibetan pig to PRRSV was compared with other two pig breeds, ZangMei black and Large White, by challenge of them with highly pathogenic PRRSV (HP-PRRSV). In the first challenge test, each eight piglets of the three breeds were inoculated with HP-PRRSV and clinical symptoms, viremia and animal mortality were examined up to 28 days post inoculation (DPI). In the secondary pathological study, each twelve piglets of the three breeds were challenged and three pigs of each breed were sacrificed on 4, 7, and 14 DPI for examination of gross damage and lung microscopic lesions. The results showed that no typical clinical signs such as cough, diarrhea and high fever were observed in challenged Tibetan pigs, which however all occurred in Large White accompanied with 40% mortality (3/8). In addition, a significant low and short viremia was detected specifically in Tibetan pigs. Based on histopathological analysis of lung sections, a mild to moderate interstitial pneumonia in Tibetan pigs and a much severe pneumonia in Large White were identified on 7-14 DPI. In summary, the study demonstrated that three genetically different pig breeds exhibited a differential host susceptibility to HP-PRRSV and Tibetan pig was much less susceptible to the virus in the three tested pig breeds.

Wang L.,Southwest University for Nationalities | Wei Y.,Sichuan Animal Science Academy
Biochemical Genetics | Year: 2012

Ferritin is a ubiquitous and conserved iron storage protein that plays a central role in iron metabolism. The ferritin heavy chain subunit (FerH) homolog was isolated from yellow grouper (Epinephelus awoara) spleen using suppression subtractive hybridization and RACE-PCR. The nucleotide sequence of FerH full-length cDNA was 1173 bp and contained an open reading frame of 534 bp, encoding a putative protein of 177 amino acids. The encoded protein shows 78-94% identity with homologs. Based on phylogenetic analysis, yellow grouper FerH is highly conserved throughout evolution and is closer to European seabass than to other species. RT-PCR analysis demonstrated that FerH was widely expressed in various healthy tissues and significantly up-regulated in liver, spleen, and anterior kidney by lipopolysaccharide. The results suggest that yellow grouper FerH may play a role in immune response. © Springer Science+Business Media, LLC 2011.

Liu J.B.,Southwest University of Science and Technology | Yang Y.K.,Sichuan Animal Science Academy | He J.,Southwest University of Science and Technology
Livestock Science | Year: 2015

The objective of this study was to investigate the effects of postweaning high-fat (HF)/high energy diets on lipid metabolism response in adipose tissue of normal birth weight (NBW) and intrauterine growth retarded (IUGR) pigs. Twenty NBW and twenty IUGR male piglets were fed either a control diet (without lard) or a HF diet (supplemented with 10% lard) from weaning (d 28) to slaughter at 111.4±2.2. kg of body weight. Feed intake and body weight of pigs were recorded monthly. Blood and backfat samples were collected at the end of the experiment and analyzed for plasma levels of metabolites and hormone, activities and mRNA expressions of enzymes involved in lipid metabolism. The results showed that plasma concentrations of leptin and cholesterol, backfat thickness, index of backfat thickness, and mRNA expressions of leptin and adipocyte differentiation-related protein (ADRP) in backfat were affected by the interaction of birth weight and postweaning diet (P<0.05). Compared with NBW littermates, IUGR pigs had lower average daily feed intake (ADFI) and average daily gain (ADG) but greater slaughter age and plasma levels of triglyceride (P<0.01). Lipid content, adipocyte diameter, activities of fatty acid synthase (FAS) and malic enzyme (ME), mRNA expressions of FAS and peroxisome proliferator-activated receptor gamma (PPARγ) in adipose tissue of IUGR pigs were greater than that of NBW pigs (P<0.01). Moreover, IUGR pigs had lower glucose-6-phosphate dehydrogenase (G-6-PDH) activity and mRNA levels of HSL compared with NBW pigs (P<0.01). Pigs fed HF diets had lower ADFI, slaughter age (P<0.01), mRNA expression abundances of LPL and FAS (P<0.01), and activities of FAS and ME (P<0.05) but greater ADG, plasma levels of triglyceride (P<0.05), and gain to feed ratio than pigs fed control diets. In summary, our results indicated that IUGR pigs had greater capability in adipose tissue lipid deposition than that of NBW offspring in response to postweaning HF/high energy diets. © 2015 Elsevier B.V.

Li X.,University of Sichuan | Cai H.,University of Sichuan | Xu J.,University of Sichuan | Ying S.,Sichuan Animal Science Academy | Zhang Y.,University of Sichuan
Amino Acids | Year: 2010

Protein-protein interactions (PPIs) play crucial roles in a number of biological processes. Recently, protein interaction networks (PINs) for several model organisms and humans have been generated, but few large-scale researches for mice have ever been made neither experimentally nor computationally. In the work, we undertook an effort to map a mouse PIN, in which protein interactions are hidden in enormous amount of biomedical literatures. Following a co-occurrence-based text-mining approach, a probabilistic model-naïve Bayesian was used to filter false-positive interactions by integrating heterogeneous kinds of evidence from genomic and proteomic datasets. A support vector machine algorithm was further used to choose protein pairs with physical interactions. By comparing with the currently available PPI datasets from several model organisms and humans, it showed that the derived mouse PINs have similar topological properties at the global level, but a high local divergence. The mouse protein interaction dataset is stored in the Mouse protein-protein interaction DataBase (MppDB) that is useful source of information for system-level understanding of gene function and biological processes in mammals. Access to the MppDB database is public available at http://bio.scu.edu.cn/mppi . © 2009 Springer-Verlag.

Zhang X.Y.,Sichuan Animal Science Academy
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2012

Best-hybridized crossing should ideally result in optimal exploitation of heterosis of lines and capitalize on expressed heterosis. Rabbit breeding is heading in the direction of breeding for best-hybridized crossing of meat rabbits. Most special sire lines are selected for post-weaning average daily gain and marketing weight. Post-weaning growth has a negative and favorable genetic correlation with the feed conversion ratio, which is used in indirect selection for feed conversion ratio. The most common selection criteria for special maternal lines are related to litter size at birth or at weaning. Since the heritability of most reproductive traits is low, we must collect as many individual and relative records as possible in the genetic evaluation of rabbits. The BLUP procedure under an animal repeatability model is the most common procedure used for evaluation of animals in selection programs for special lines of meat rabbits. Direct selection for litter size is less efficient than selection for post-weaning growth, but the estimation of heterosis is generally higher for litter size than that for the post-weaning growth. Evaluation of heterosis could be performed by estimating crossbreeding parameters in the cross or comparing contemporary productivity among purebreds and crossbreds. Here, we reviewed breeding of special lines, exploitation of heterosis of crossbreds, and establishment of crossbreeding system of hybrid meat rabbits and summarized the methodologies of breeding special lines, criteria in selection programs, and the result of heterosis estimates.

Liu J.B.,Southwest University of Science and Technology | Yang Y.K.,Sichuan Animal Science Academy | He J.,Southwest University of Science and Technology | Zeng F.K.,Southwest University of Science and Technology
Livestock Science | Year: 2014

Two experiments were conducted to compare 2 types of basal diet (semi-purified cornstarch diet and corn-based practical diet) in the determination of true P digestibility (TPD) in soybean (SBM) and canola (CM) meals for growing pigs by the regression method. In each study, forty-eight 25-kg barrows were assigned to 6 dietary treatments arranged in a 2×3 factorial of 2 types of diet (semi-purified cornstarch diet or corn-based practical diet), and 3 graded levels of SBM (Exp. 1) or CM (Exp. 2) at 120, 240, or 360. g/kg. Both experiments consisted of a 5-d adjustment period and a 7-d total collection period with ferric oxide used as a marker to indicate the initiation and termination of fecal collection. Our results showed that dietary P intake, fecal P output, digested P, and retained P were increased linearly with graded increasing levels of SBM or CM for growing pigs in each type of diet (P<0.01). Regression of digested P against dietary P intake resulted in the estimated TPD in SBM for pigs fed the semi-purified cornstarch diet and the corn-based practical diet to be 40.01 and 37.49%, respectively. The respective estimates of TPD in CM were calculated to be 30.19 and 27.22% for pigs fed the semi-purified diet and the practical diet. There was no difference in the estimates of TPD in SBM and CM between pigs fed the semi-purified diet and the practical diet. In summary, our data indicated that diet type (semi-purified or practical diet) does not influence the determination of TPD in assay ingredients for growing pigs by the regression method. Therefore, a semi-purified cornstarch diet can be used for determination of P digestibility. © 2014 Elsevier B.V.

Gu Y.R.,Sichuan Animal Science Academy
Genetics and molecular research : GMR | Year: 2012

Determination of an optimal set/number of internal control microRNA (miRNA) genes is a critical, but often undervalued, detail of quantitative gene expression analysis. No validated internal genes for miRNA quantitative PCR (q-PCR) in pig milk were available. We compared the expression stability of six porcine internal control miRNA genes in pig milk from different lactation periods (1 h, 3 days, 7 days, 14 days, 21 days, and 28 days postpartum), using an EvaGreen q-PCR approach. We found that using the three most stable internal control genes to calculate the normalization factor is sufficient for producing reliable q-PCR expression data. We also found that miRNAs are superior to ribosomal RNA (rRNA) and snRNA, which are commonly used as internal controls for normalizing miRNA q-PCR data. In terms of economic and experimental feasibility, we recommend the use of the three most stable internal control miRNA genes (miR-17, -107 and -103) for calculating the normalization factors for pig milk samples from different lactation periods. These results can be applied to future studies aimed at measuring miRNA abundance in porcine milk.

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