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Wang Y.,Sichuan Agricultural University | Qiu M.,Sichuan Animal Science Academy | Yang J.,Sichuan Agricultural University | Zhao X.,Sichuan Agricultural University | And 2 more authors.
Infection, Genetics and Evolution | Year: 2014

The major histocompatibility complex (MHC) among chickens has been well established as being associated with disease resistance and pathogens infection, but the genetic differences in MHC between chickens susceptible to certain infections and those chickens that remain uninfected have not been sufficiently determined. In this study, we sought the genetic basis that may underlie differences in susceptibility to infection among chickens by challenging four groups of broilers with Marek's disease virus (MDV). Over the course of the experiment, lesions began to appear between 21 and 35. days post challenge (dpc), and commercial broilers were not necessarily better than indigenous chickens in terms of disease resistance. The four groups showed neutral resistance to MDV infection validated by challenge results and evolutionary analysis of exons 2 and 3 of the MHC class I region. Several variable sites in exon 2 and exon 3 were exclusively appeared in infected chickens. Exon 3 was likely more crucial than exon 2 in disease resistance. Our observations offered a support for a potential association between promiscuous pathogens and conspicuous genetic diversity in the MHC class I region. © 2013 Elsevier B.V. Source


Liu J.B.,Southwest University of Science and Technology | Yang Y.K.,Sichuan Animal Science Academy | He J.,Southwest University of Science and Technology
Livestock Science | Year: 2015

The objective of this study was to investigate the effects of postweaning high-fat (HF)/high energy diets on lipid metabolism response in adipose tissue of normal birth weight (NBW) and intrauterine growth retarded (IUGR) pigs. Twenty NBW and twenty IUGR male piglets were fed either a control diet (without lard) or a HF diet (supplemented with 10% lard) from weaning (d 28) to slaughter at 111.4±2.2. kg of body weight. Feed intake and body weight of pigs were recorded monthly. Blood and backfat samples were collected at the end of the experiment and analyzed for plasma levels of metabolites and hormone, activities and mRNA expressions of enzymes involved in lipid metabolism. The results showed that plasma concentrations of leptin and cholesterol, backfat thickness, index of backfat thickness, and mRNA expressions of leptin and adipocyte differentiation-related protein (ADRP) in backfat were affected by the interaction of birth weight and postweaning diet (P<0.05). Compared with NBW littermates, IUGR pigs had lower average daily feed intake (ADFI) and average daily gain (ADG) but greater slaughter age and plasma levels of triglyceride (P<0.01). Lipid content, adipocyte diameter, activities of fatty acid synthase (FAS) and malic enzyme (ME), mRNA expressions of FAS and peroxisome proliferator-activated receptor gamma (PPARγ) in adipose tissue of IUGR pigs were greater than that of NBW pigs (P<0.01). Moreover, IUGR pigs had lower glucose-6-phosphate dehydrogenase (G-6-PDH) activity and mRNA levels of HSL compared with NBW pigs (P<0.01). Pigs fed HF diets had lower ADFI, slaughter age (P<0.01), mRNA expression abundances of LPL and FAS (P<0.01), and activities of FAS and ME (P<0.05) but greater ADG, plasma levels of triglyceride (P<0.05), and gain to feed ratio than pigs fed control diets. In summary, our results indicated that IUGR pigs had greater capability in adipose tissue lipid deposition than that of NBW offspring in response to postweaning HF/high energy diets. © 2015 Elsevier B.V. Source


Wang L.,Southwest University for Nationalities | Wei Y.,Sichuan Animal Science Academy
Biochemical Genetics | Year: 2012

Ferritin is a ubiquitous and conserved iron storage protein that plays a central role in iron metabolism. The ferritin heavy chain subunit (FerH) homolog was isolated from yellow grouper (Epinephelus awoara) spleen using suppression subtractive hybridization and RACE-PCR. The nucleotide sequence of FerH full-length cDNA was 1173 bp and contained an open reading frame of 534 bp, encoding a putative protein of 177 amino acids. The encoded protein shows 78-94% identity with homologs. Based on phylogenetic analysis, yellow grouper FerH is highly conserved throughout evolution and is closer to European seabass than to other species. RT-PCR analysis demonstrated that FerH was widely expressed in various healthy tissues and significantly up-regulated in liver, spleen, and anterior kidney by lipopolysaccharide. The results suggest that yellow grouper FerH may play a role in immune response. © Springer Science+Business Media, LLC 2011. Source


Zhang X.Y.,Sichuan Animal Science Academy
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2012

Best-hybridized crossing should ideally result in optimal exploitation of heterosis of lines and capitalize on expressed heterosis. Rabbit breeding is heading in the direction of breeding for best-hybridized crossing of meat rabbits. Most special sire lines are selected for post-weaning average daily gain and marketing weight. Post-weaning growth has a negative and favorable genetic correlation with the feed conversion ratio, which is used in indirect selection for feed conversion ratio. The most common selection criteria for special maternal lines are related to litter size at birth or at weaning. Since the heritability of most reproductive traits is low, we must collect as many individual and relative records as possible in the genetic evaluation of rabbits. The BLUP procedure under an animal repeatability model is the most common procedure used for evaluation of animals in selection programs for special lines of meat rabbits. Direct selection for litter size is less efficient than selection for post-weaning growth, but the estimation of heterosis is generally higher for litter size than that for the post-weaning growth. Evaluation of heterosis could be performed by estimating crossbreeding parameters in the cross or comparing contemporary productivity among purebreds and crossbreds. Here, we reviewed breeding of special lines, exploitation of heterosis of crossbreds, and establishment of crossbreeding system of hybrid meat rabbits and summarized the methodologies of breeding special lines, criteria in selection programs, and the result of heterosis estimates. Source


Wang L.,Southwest University | Fan J.,Southwest University | Fan J.,Sichuan Animal Science Academy | Yu M.,Southwest University | And 3 more authors.
Molecular Biology Reports | Year: 2011

As one of the transport systems on the sperm plasma membrane, CD4 molecule plays a distinct role in the process of sperm/DNA interaction. This makes it possible to explain the mechanism of sperm-mediated gene transfer (SMGT), which at present is still a mystery in this area. In this study, seminal samples of 60 individuals from seven breed bucks were collected to detect the ability of sperm in internalizing exogenous DNA, and genomic DNA from 147 individual blood samples (including 60 bucks referred ahead) were extracted to test the polymorphisms of CD4 genes by using PCR-SSCP technique. Then the correlation between them was evaluated. The results showed that: (1) it was a novel finding that breed-dependence of exogenous DNA binding to goat spermatozoa. There was the most significant difference among the buck breeds of sperm in binding exogenous DNA (F(6, 53) = 4.811, P = 0.001) and in internalizing them into nuclei (F(6, 53) = 4.587, P = 0.001). The ability of Lezhi Black goat was the lowest (P < 0.01) among the seven breeds. (2) There was no significant correlation between the ability of sperm in internalizing exogenous DNA and each semen quality parameter (P > 0.05). (3) In particular, three single-nucleotide polymorphisms (SNP) were described and there was one SNP (G/A700) of CD4 gene that made G234R substitution in the amino acid sequence of CD4 molecule. Nanjiang Yellow goat and Lezhi Black goat had higher hereditary variation compared with other breeds. (4) CD4 polymorphisms were highly associated with the ability of sperm in internalizing exogenous DNA. The SNP of Caprine CD4 gene exon 6 might be an important molecular marker of the ability to internalize exogenous DNA into sperm. © 2010 Springer Science+Business Media B.V. Source

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