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Yucheng, China

Sichuan Agricultural University is a university located in Ya'an city, Sichuan province, China and part of the “Project 211”,“PLANT 111”,“plant 2011”, specialized in biotechnology and agricultural science, and as well as offering degrees in physical science, engineering, economics, management, veterinary medicine, liberal arts, pedagogy and law. Wikipedia.

Liu M.,Sichuan Agricultural University
Frontiers in cellular and infection microbiology | Year: 2013

Bartonella are hemotropic bacteria responsible for emerging zoonoses. Most Bartonella species appear to share a natural cycle that involves an arthropod transmission, followed by exploitation of a mammalian host in which they cause long-lasting intra-erythrocytic bacteremia. Persistence in erythrocytes is considered an adaptation to transmission by bloodsucking arthropod vectors and a strategy to obtain heme required for Bartonella growth. Bartonella genomes do not encode for siderophore biosynthesis or a complete iron Fe(3+) transport system. Only genes, sharing strong homology with all components of a Fe(2+) transport system, are present in Bartonella genomes. Also, Bartonella genomes encode for a complete heme transport system. Bartonella must face various environments in their hosts and vectors. In mammals, free heme and iron are rare and oxygen concentration is low. In arthropod vectors, toxic heme levels are found in the gut where oxygen concentration is high. Bartonella genomes encode for 3-5 heme-binding proteins. In Bartonella henselae heme-binding proteins were shown to be involved in heme uptake process, oxidative stress response, and survival inside endothelial cells and in the flea. In this report, we discuss the use of the heme uptake and storage system of B. henselae during its infection cycle. Also, we establish a comparison with the iron and heme uptake systems of Yersinia pestis used during its infection cycle. Source

Nuclear localization signals (NLSs) and nuclear export signals (NESs) are important intramolecular regulatory elements for protein nucleocytoplasmic trafficking. This regulation confers spatial specificity to signal initiation and transduction in eukaryotic cells and thus is fundamental to the viability of all eukaryotic organisms. Here, we developed a simple and rapid method in which activity of putative NLSs or NESs was reported by subcellular localization of two tandem fluorescent proteins in fusion with the respective NLSs or NESs after agroinfiltration-mediated transient expression in leaves of Nicotiana benthamiana (Nb). We further demonstrated that the predicted NES from amino acid residue (aa) 9 to 22 and the NLS from aa91 to 101 in the broad-spectrum disease resistance protein RPW8.2 possess nuclear export and import activity, respectively. Additionally, by testing overlapping fragments covering the full length of RPW8.2, we identified another NLS from aa65 to 74 with strong nuclear import activity and two tandem non-canonical NESs in the C-terminus with strong nuclear export activity. Taken together, our results demonstrated the utility of a simple method to evaluate potential NLSs and NESs in plant cells and suggested that RPW8.2 may be subject to opposing nucleocytoplasmic trafficking forces for its subcellular localization and functional execution. Source

Jiang Y.Z.,Sichuan Agricultural University
Genetics and molecular research : GMR | Year: 2012

We evaluated carcass and meat quality traits of two Chinese native crossbreeds Landrace x Meishan (LM) and Duroc x (Landrace x Meishan) (DLM) and two foreign crossbreeds Duroc x (Landrace x Yorkshire) (DLY) and PIC (an imported five-way crossbreed). One hundred and twenty weaned pigs (half castrated males and half females) were reared and slaughtered at a predestinated slaughter age. The general carcass and meat quality traits were measured and analyzed. The DLY and PIC crosses had significantly heavier live weights (93.39 and 96.33 kg, P < 0.01), significantly higher dressing percentages (80.65 and 79.39%, P < 0.05), significantly bigger loin areas (42.69 and 43.91 cm(2), P < 0.001), and significantly more lean carcasses (65.78 and 66.40%, P < 0.001) than LM and DLM. On the other hand, LM had a significantly lower live weight (70.29 kg, P < 0.01), significantly thicker back fat (3.54 cm, P < 0.001), significantly less lean carcasses (46.82%, P < 0.001), and significantly less ham and breech (26.53%, P < 0.05) than the other crossbreeds. Among meat quality parameters, LM had the highest intramuscular fat content (5.02%, P < 0.001) and the smallest fiber area (3126.45 μm(2), P < 0.01). However, PIC showed the lowest pH(1) (5.82, P < 0.01) and pH(2) (5.63, P < 0.01), the highest drip loss (2.89%, P < 0.01), and the lowest intramuscular fat (1.35%, P < 0.001). We concluded that LM and DLM had good meat quality traits but poorer carcass traits than DLY and PIC; DLY had good carcass and meat quality traits; PIC had good carcass traits, but it had less intramuscular fat, lower pH and higher drip loss. Source

Yuan S.,Sichuan Agricultural University
Cell Death and Disease | Year: 2013

New treatments and new drugs for avian influenza virus (AIV) infection are developed continually, but there are still high mortality rates. The main reason may be that not all cell death pathways induced by AIV were blocked by the current therapies. In this review, drugs for AIV and associated acute respiratory distress syndrome (ARDS) are summarized. The roles of antioxidant (vitamin C) and multiple immunomodulators (such as Celecoxib, Mesalazine and Eritoran) are discussed. The clinical care of ARDS may result in ischemia reperfusion injury to poorly ventilated alveolar cells. Cyclosporin A should effectively inhibit this kind of damages and, therefore, may be the key drug for the survival of patients with virus-induced ARDS. Treatment with protease inhibitor Ulinastatin could also protect lysosome integrity after the infection. Through these analyses, a large drug combination is proposed, which may hypothetically greatly reduce the mortality rate. © 2013 Macmillan Publishers Limited. All rights reserved. Source

Wang X.,Sichuan Agricultural University
Clean - Soil, Air, Water | Year: 2011

Magnetic hydroxyapatite (HAP), which combined superparamagnetic Fe3O4 nanoparticles and HAP, composite materials were prepared by ultrasound method in this paper. It has also been found that these materials have the ability to adsorb phenol in wastewater. The magnetic materials were investigated by scanning electron microscope, X-ray diffraction (XRD), Fourier transform infrared spectroscopy, thermal gravimetric analysis, vibrating sample magnetometer, and N2 adsorption in order to elucidate the morphology, structure, and other properties. When the prepared magnetic materials were calcined at 200°C, the prepared Fe3O4 was oxidized to Fe2O3, possessing loose-shaped holes with a high specific area of 325.2m2/g, a magnetization intensity of 12.5emu/g, and the N2 adsorption isotherm belongs to porous adsorption type I. Moreover, the magnetic HAP can adsorb 90% phenol in wastewater. This means that it is an excellent recyclable phenol sorbent for sewage treatment. Experiments confirmed that the Freundlich adsorption isotherms model applies to lower phenol concentrations (0-50mg/L), while for high phenol concentrations (50-500mg/L) the Langmuir adsorption isotherms model fits. The magnetic sorbents have the capacity to regenerate after reaching adsorption saturation using ethanol as eluant and external magnetic field as separation unit. The efficiency of adsorption was reduced only by 10% over a six time use period. The synthesis of a recyclable magnetic Fe2O3 at HAP sorbent is described here. The magnetic materials specific surface showed good adsorption capacities for phenol. Therewith, an effective, economic, and green method to remove phenol in wastewater is given. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

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