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Bai Y.,Southern Medical University | Bai Y.,Sichuan Academy of Medical Science | Sun Y.,Southern Medical University | Sun Y.,Guangdong Medical College | And 6 more authors.
Oncotarget | Year: 2014

Secretagogin (SCGN) has recently been identified to play a crucial role in cell apoptosis, receptor signaling and differentiation. However, its clinical significance and functional roles in SCLC chemoresistance remain unknown. Here we examined the expression of SCGN in clinical samples from SCLC patients and evaluated its relation with clinical prognosis. Then up and down-regulation of SCGN were carried out in SCLC cell lines to assess its influence on chemoresistance. Furthermore, luciferase reporter assay was used to evaluate whether SCGN is a novel direct target of miR-494. Our results revealed that elevated expression of SCGN was correlated with the poorer prognosis of SCLC patients and the more significant correlation with chemosensitivity. We also found that knockdown of SCGN expression in H69AR and H446AR cells increased chemosensitivity via increasing cell apoptosis and cell cycle arrest of G0/G1 phase, while over-expression of SCGN reduced chemosensitivity in sensitive H69 and H446 cells. SCGN as a novel target of miR-494 by luciferase reporter assay, up-regulation of miR-494 can sensitize H69AR cells to chemotherapeutic drugs. These results suggest SCGN is involved in the chemoresistance of SCLC under the regulation of miR-494 and may be a potential biomarker for predicting therapeutic response in treatment SCLC. Source

Xiong F.,Sichuan Academy of Medical Science
PLoS ONE | Year: 2015

Hepatocellular carcinoma is one of the most common malignancies worldwide, with a high risk of portal vein tumor thrombus (PVTT). Some promising results have been achieved for venous metastases of hepatocellular carcinoma; however, the etiology of PVTT is largely unknown, and it is unclear why the incidence of PVTT is not proportional to its distance from the carcinoma. We attempted to address this issue using physical concepts and mathematical tools. Finally, we discuss the relationship between the probability of a collision event and the microenvironment of the PVTT. Our formulae suggest that the collision probability can alter the tumor microenvironment by increasing the number of tumor cells. © 2015 Fei Xiong. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Wen H.-L.,Central South University | Wen H.-L.,Sichuan Academy of Medical Science | Liang Z.-S.,Central South University | Zhang R.,The Seventh Peoples Hospital of Chengdu | Yang K.,Central South University
Cardiovascular Diabetology | Year: 2013

Aims: Given the importance of inflammation in the onset and progression of diabetic cardiomyopathy, we investigated the potential protective effects of triptolide, an anti-inflammatory agent, in streptozotocin-induced diabetic rat model and in H9c2 rat cardiac cells exposed to high glucose. Methods and results: Diabetic rats were treated with triptolide (100, 200, or 400 μg/kg/day respectively) for 6 weeks. At the end of this study, after cardiac function measurements were performed, rats were sacrificed and their hearts were harvested for further histologic and molecular biologic analysis. Enhanced activity and expression of nuclear factor-kappaB (NF-κB) p65 in diabetic hearts were associated with increased inflammatory response, as demonstrated by increased pro-inflammatory cytokines, cell adhesion molecules and invading inflammatory cells, as well as increased fibrosis, in line with impaired left ventricular function. Triptolide attenuated these morpho-functional alterations. Furthermore, triptolide (20 ng/ml) also attenuated high glucose-induced inflammation in H9c2 rat cardiac cells. Conclusion: Our data demonstrate that anti-inflammatory effects of triptolide involving the NF-κB signaling pathway can improve left ventricular function under diabetic conditions, suggesting triptolide treatment might be beneficial in diabetic cardiomyopathy. © 2013 Wen et al.; licensee BioMed Central Ltd. Source

Liu K.,Chinese University of Hong Kong | Chen L.J.,Chinese University of Hong Kong | Lai T.Y.Y.,Chinese University of Hong Kong | Tam P.O.S.,Chinese University of Hong Kong | And 6 more authors.
Ophthalmology | Year: 2014

Purpose To investigate the associations of genetic variants in the high-density lipoprotein (HDL) metabolism pathway with neovascular age-related macular degeneration (AMD) and polypoidal choroidal vasculopathy (PCV). Design Cross-sectional, case-control association study. Participants A Chinese case-control group of 200 neovascular AMD patients, 233 PCV patients, and 275 control subjects. Methods Eight single nucleotide polymorphisms (SNPs) from 6 genes of the HDL metabolism pathway and 2 known AMD-associated SNPs, rs800292 (from complement factor H [CFH]) and rs11200638 (from HtrA serine peptidase 1 [HTRA1]), were genotyped in all study subjects using the TaqMan genotyping technology (Applied Biosystems, Foster City, CA). Main Outcome Measures Allele and genotypic frequencies of selected SNPs. Results The SNP rs3764261 in the cholesteryl ester transfer protein (CETP) gene was associated significantly with neovascular AMD (P = 1.82×10-4; odds ratio [OR], 1.89) and PCV (P = 4.04×10-4; OR, 1.80). The associations remained significant after adjusting for the CFH SNP rs800292 and the HTRA1 SNP rs11200638. A significant interaction between the CETP SNP rs3764261 and the CFH SNP rs800292 existed in both neovascular AMD and PCV, the rs800292 G allele conferring a significantly increased risk of the diseases only in individuals carrying the risk allele T of rs3764261. A borderline association was detected between the ATP-binding cassette, subfamily G, member 1 (ABCG1) gene SNP rs57137919 and PCV (P = 0.03). Conclusions Our results showed that CETP is a susceptibility gene for neovascular AMD and PCV and that ABCG1 a putative gene for PCV. CETP exerts a modifying effect on CFH in the genetic risk. Our data suggest a link of the HDL metabolism pathway with neovascular AMD and PCV. © 2014 by the American Academy of Ophthalmology Published by Elsevier Inc. Source

Xiao H.T.,Sichuan Academy of Medical Science
European review for medical and pharmacological sciences | Year: 2012

Asthma is a major epidemic affecting up to one third people in developed countries over the last decades, and making a crucial impact on morbidity rates. The classical characters of asthma in human are airway inflammation, airway hyperreactivity, and airway remodeling. Hygiene hypothesis, inflammation cells and signaling pathway in asthma were involved in Toll-like receptors (TLRs). TLRs are a kind of pattern recognition receptors, which are important in recognition of various pathogens. TLRs have been seen as a key target for asthma treatment, so a promising approach for asthma treatment was adopted to the multiwayly modulating toll-like receptors way.   Source

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