Sichuan Academy of Agriculture Science

Chengdu, China

Sichuan Academy of Agriculture Science

Chengdu, China
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Wang Y.,China Agricultural University | Xie J.,CAS Institute of Genetics and Developmental Biology | Zhang H.,China Agricultural University | Guo B.,China Agricultural University | And 18 more authors.
Theoretical and Applied Genetics | Year: 2017

Key message: A stripe rust resistance gene YrZH22 was mapped by combined BSR-Seq and comparative genomics analyses to a 5.92 centimorgan (cM) genetic interval spanning a 4 Mb physical genomic region on wheat chromosome 4BL1.Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases of wheat and severely threatens wheat production worldwide. The widely grown Chinese wheat cultivar Zhoumai 22 is highly resistant to the current prevailing PST race CYR34 (V26). Genetic analysis of F5:6 and F6:7 recombinant inbred line (RIL) populations indicated that adult-plant stripe rust resistance in Zhoumai 22 is controlled by a single gene, temporarily designated YrZH22. By applying bulked segregant RNA-Seq (BSR-Seq), 7 SNP markers were developed and SNP mapping showed that YrZH22 is located between markers WGGB105 and WGGB112 on chromosome arm 4BL. The corresponding genomic regions of the Chinese Spring 4BL genome assembly and physical map of Aegilops tauschii 4DL were selected for comparative genomics analyses to develop nine new polymorphic markers that were used to construct a high-resolution genetic linkage map of YrZH22. YrZH22 was delimited in a 5.92 cM genetic interval between markers WGGB133 and WGGB146, corresponding to 4.1 Mb genomic interval in Chinese Spring 4BL and a 2.2 Mb orthologous genomic region in Ae. tauschii 4DL. The genetic linkage map of YrZH22 will be valuable for fine mapping and positional cloning of YrZH22, and can be used for marker-assisted selection in wheat breeding. © 2017 Springer-Verlag GmbH Germany


Liu Z.,University of Electronic Science and Technology of China | Liu Z.,Sichuan Academy of Agriculture science | Zhang Y.,University of Electronic Science and Technology of China | Zhang Y.,Sichuan Agricultural University | And 5 more authors.
African Journal of Biotechnology | Year: 2011

This study is carried out to analyze the genetic diversity of red spider mite-resistant cotton (Gossypium hirsutum) cultivars that are applied in cultivar identification and breeder's right protection of cottons. The genomic DNA was used as template and random primers were used to analyze the genetic diversity of 21 accessions of Gossypium hirsutum by RAPD-PCR. Among the 100 primers screened, 20 primers could generate 176 fragments, 96.02% of which were polymorphic ones. The similarity coefficient of cultivars was between 0.2273 - 0.9773. The average values of effective number of alleles, Nei's gene diversity and Shannon's information index were 1.7391, 0.4017, and 0.5773, respectively. Cluster analysis based on UPGMA revealed that 21 cultivars could be divided into three groups. The analysis revealed that it was corresponding to the geographical distribution and most of the lines had a wide genetic base. © 2011 Academic Journals.


Liu Z.-H.,University of Electronic Science and Technology of China | Liu Z.-H.,Sichuan Academy of Agriculture science | Zhang Y.,University of Electronic Science and Technology of China | Zhang Y.,Sichuan Agricultural University | And 5 more authors.
African Journal of Biotechnology | Year: 2010

In this study, amplified fragment length polymorphism (AFLP) technique was used to analyze the genealogical relationship of 7 introgressed cotton varieties from the hybridization of upland (Gossypium hirsutum L) and sea-island cottons (Gossypium barbadense L). Ten pairs of primer combinations with high polymorphism and resolution were selected from 64 primers. These 10 primer combinations resulted in a total of 480 bands, of which 374 bands (77.9%) were polymorphic and 51 bands (10.6%) were specific. Cluster analyses showed that 7 varieties of cottons were divided into two groups. Our results suggested that interspecific hybridization is feasible to broaden germplasm of upland cotton and AFLP technique could be applied for the identification of variety purity and genealogical relationship. © 2010 Academic Journals.


Yang E.N.,Sichuan Academy of Agriculture science | Zou Y.C.,Sichuan Academy of Agriculture science | Yang W.Y.,Sichuan Academy of Agriculture science | Tang Y.L.,Sichuan Academy of Agriculture science | And 2 more authors.
Czech Journal of Genetics and Plant Breeding | Year: 2011

Sichuan is an important wheat producing province of China where severe stripe rust epidemics occur annually. Developing high-yielding wheat varieties with good and stable stripe rust resistance is a foremost breeding objective of all breeding programs. Because minor gene based adult-plant resistance (APR) is considered durable, a shuttle breeding program between Sichuan Academy of Agricultural Sciences (SAAS) and CIMMYT was initiated in 2000 to transfer APR identified in CIMMYT wheats to wheat germplasm adapted in Sichuan. During 2007-2009, a total of 669 advanced generation lines obtained from this shuttle breeding effort were provided to the Plant Protection Research Institute, SAAS for official multi-environment stripe rust tests, and 231 elite lines were characterized for yield performance by the agronomists at the Crop Research Institute, SAAS. Between 11-39% lines were highly resistant depending on the year of testing and 17 (7.3%) lines had 5% or higher yields than the check mean. The adapted resistant lines are being used by various breeding programs to enhance resistance diversity, and three lines are being tested in National or Provincial Yield Trials for possible releases.


Zhang F.,Sichuan Academy of Agriculture Science | Jiang W.,University of Sichuan | Wang X.,University of Sichuan | Huang J.,Sichuan University | And 4 more authors.
Fresenius Environmental Bulletin | Year: 2011

Alcoholic wastewater was used as culture medium to biosynthesize bioflocculant after some bioflocculant-producing strains were isolated from activated sludge in a wastewater treatment plant. Two strains with the highest flocculating rates were identified as Bacillus cereus and Pichia membranifaciens. The highest flocculating rate of mix-strains was up to 88.0% within 24 hours, and the bioflocculant production period of mix-strains was only half the time compared to that associated with a single strain. The optimal culture conditions of mix-strains were studied, and the bioflocculant was produced under these conditions. The bioflocculant had characteristics of polysaccharides and its total polysaccharide content was up to 63.4% (w/w). The homogeneous monosaccharide in it was glucose with a concentration of 40.1% (w/w) of polysaccharides. However, the total protein was only 0.87% (w/w). The bioflocculant was thermo-stable and its molecular weight was 50798 Da. The bond between the bioflocculant and kaolin granule was an electrovalent bond, and bridging effect occurred during the process of flocculating. © by PSP.


He X.-L.,Guangdong Institute of Microbiology | He X.-L.,Sichuan Academy of Agriculture science | Li T.-H.,Guangdong Institute of Microbiology | Xi P.-G.,South China Agricultural University | And 2 more authors.
Fungal Diversity | Year: 2013

Taxonomy and phylogeny of Entoloma s.l. subgenus Pouzarella in China are carried out based on morphological characters and molecular data. Seven species in subg. Pouzarella are recognized in China and a key to these species is provided. Five species, Entoloma changchunense, E. crassicystidiatum, E. furfuraceum, E. subaraneosum and E. tenuissimum, are described as new; a new Chinese record E. dysthaloides is also presented in this paper. Among them, E. crassicystidiatum was discovered from southern China, and the others were found in northeastern China. Molecular phylogeny of subg. Pouzarella were conducted based on nLSU, mtSSU and RPB2, and the phylogenetic positions of the five new species are discussed. The placement of subg. Pouzarella in Entoloma s.l. clade is well resolved in all analyses presented here, and the monophyly of subg. Pouzarella is strongly supported by the morphological and molecular phylogeny. The infrageneric classifications in this group were not well recovered in the present study; and further studies based on more samples and gene loci are needed to a better understanding of the infrageneric taxa in subg. Pouzarella in the future. © 2012 Mushroom Research Foundation.

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