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Liu Y.-X.,Sichuan Agricultural University | Sun X.-H.,Sichuan Agricultural University | Xu B.-L.,Sichuan Agricultural Academy of Science | Chen G.-Y.,Sichuan Agricultural University
Journal of Plant Sciences

Plant architecture is governed by the action of meristems. During vegetative development, the shoot apical meristem is responsible for initiating all of the above-ground structures including the nodes, internodes, leaves, axillary meristems and the inflorescence. Five barley mutants with low-tiller have been found, currently including, low number of tillersl (Intl.a), absent lower lateralsl (alsl), intermedium-b (int-b), uniculm2 (cul2.b), uniculm4 (cul4) and semi brachytic (uzu). Specifically, the cul2.b mutant failed to develop tillers, while the Intl.a mutant can produce 1-4 tillers. Genetic analysis indicated that two mutant phenotypes were caused by two recessive genes cul2.b and Intl.a, respectively. In this study, two F2 populations, 279 individuals derived from Bowmanx GSHO 531 and 184 individuals derived from BowmanxGSHO 1984 were developed for mapping the cul2.b and lntl. a genes using Simple Sequence Repeats (SSR) markers. F3 populations were created to identify genotypes of F2 individuals. Ultimately, cul2.b was located between SSR markers GBM1212 and Bmag 0613 on the long arm of chromosome 6H, with distances of 12.7 and 13.2 cm to the two markers, respectively. Another five SSR markers (GBM 1319, GBM 1423, Bmag 0807, Bmag 0378 and Bmag 0003) on chromosome 6H were also found around the cul2 gene, with distances of 19.6, 33.3, 34.1, 71.5 and 80 cm to the cul2.b gene. The Intl.a gene was positioned 7.8 cm away from GBM 1043 on chromosome 3H. This study narrowed the block of tiller development gene in the cul2 and lntl mutant. It is a benefit for further map-based clone of the genes. © 2012 Academic Journals Inc. Source

Yang L.-J.,Sichuan Agricultural University | Yang L.-J.,Xinxiang Institute of Agriculture | Liu Y.-X.,Sichuan Agricultural University | Xu B.-L.,Sichuan Agricultural Academy of Science | And 2 more authors.
Asian Journal of Biochemistry

One hundred and thirteen low-molecular-weight glutenin subunits encoding sequences and parts of upstream were characterized from Triticum dicoccoides. The encoded proteins of 113 genes had similar structures to previously characterized LMW-GS. These sequences had 856~1402 nucleotides in length with 2~26 repeat motifs. Most of the sequences were typical LMW-m glutenin subunits genes and the frequency of SNPs was 1.6 out of 10 bases and A-G mutation was the most frequent. Fourteen deduced amino acid sequences were found to be possessed with an additional cysteine residue in C-ter I. Thirty-six haplotypes were detected and phylogenetic analysis indicated that the 36 haplotypes could be classified into 3 haplotype groups. Individually classifications based on the four main domains of LMW-GS DNA sequences, 5'flanking, single peptide, N-terminals and C-terminal, were in agreement with the classification based on the coding regions. Consilient evolution was found between domains of LMW-GS as well as each domain and the whole coding region. The results revealed the important information of low-molecular-weight glutenin subunit gene family and contributed to our understanding of functional aspects of the low-molecular-weight glutenin subunit genes. © 2011 Academic Journals Inc. Source

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