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Olennikov D.N.,Russian Academy of Sciences | Tankhaeva L.M.,Russian Academy of Sciences | Rokhin A.V.,Irkutsk State University | Agafonova S.V.,Siberian Institute of Plant Physiology and Biochemistry
Chemistry of Natural Compounds | Year: 2012

Six pigment fractions (total yield 52.13% of raw material mass) were isolated by fractionation of polymeric components of melanin from Inonotus obliquus (Pers.) Pil. sclerotia. The isolated fractions differed in degree of aromaticity, molecular-weight distribution, and content of functional groups according to elemental analysis; UV, IR, and 13C NMR spectroscopy; and gel chromatography. The dominant components were highly aromatic polymers of molecular weight 2-20 kDa with a high content of carboxylic and phenolic hydroxyls. It was found that fractions with a high degree of aromaticity and content of pyrocatechol groups exhibited pronounced antioxidant activity. © 2012 Springer Science+Business Media, Inc.

Olennikov D.N.,Institute of General and Experimental Biology | Dudareva L.V.,Siberian Institute of Plant Physiology and Biochemistry | Osipenko S.N.,Siberian Institute of Plant Physiology and Biochemistry | Penzina T.A.,Russian Academy of Sciences
Journal of the Serbian Chemical Society | Year: 2010

The essential oils from five samples of leaves of Rhododendron aureum from the Irkutsk region, Pribaikal'e, Russian Federation, were isolated by hydrodistillation and analyzed by a combination of GC and GC/MS. Compounds representing 70.5-78.3 % of the oils were identified. Twenty-seven compounds were identified according to their chromatographic retention indices and mass spectra. The major components of the oils were calarene (10.4-66.4 %), β-bourbonene (0.5-27.4 %), a-selinene (2.1-8.0 %) and kaur-16-ene (2.0-6.3 %). It was found that the chemical composition of Rh. aureum essential oil depends on the altitude of the growing plants.

Fogelqvist J.,Swedish University of Agricultural Sciences | Verkhozina A.V.,Siberian Institute of Plant Physiology and Biochemistry | Katyshev A.I.,Siberian Institute of Plant Physiology and Biochemistry | Pucholt P.,Swedish University of Agricultural Sciences | And 4 more authors.
BMC Evolutionary Biology | Year: 2015

Background: Hybridization and introgression are said to occur relatively frequently in plants, and in particular among different species of willows. However, data on the actual frequency of natural hybridization and introgression is rare. Here, we report the first fine-scale genetic analysis of a contact zone shared between the three basket willow species, Salix dasyclados, S. schwerinii and S. viminalis in the vicinity of the Lake Baikal in Southern Siberia. Individuals were sampled in fourteen populations and classified as pure species or hybrids based on a set of morphological characters. They were then genotyped at 384 nuclear SNP and four chloroplast SSR loci. The STRUCTURE and NewHybrids softwares were used to estimate the frequency and direction of hybridization using genotypic data at the nuclear SNP loci. Results: As many as 19 % of the genotyped individuals were classified as introgressed individuals and these were mainly encountered in the centre of the contact zone. All introgressed individuals were backcrosses to S. viminalis or S. schwerinii and no F1 or F2 hybrids were found. The rest of the genotyped individuals were classified as pure species and formed two clusters, one with S. schwerinii individuals and the other with S. viminalis and S. dasyclados individuals. The two clusters were significantly genetically differentiated, with F ST ∈=∈0.333 (0.282-0.382, p∈<∈0.001). In contrast, for the chloroplast haplotypes, no genetic differentiation was observed as they were completely shared between the species. Based on morphological classification only 5 % of the individuals were classified as introgressed individuals, which was much less than what was detected using genotypic data. Conclusions: We have discovered a new willow hybrid zone with relatively high frequency of introgressed individuals. The low frequency of F1 hybrids indicates that ongoing hybridization is limited, which could be because of the presence of reproductive barriers or simply because the conditions are not favorable for hybridization. We further conclude that in order to get a complete picture of the species composition of a hybrid zone it is necessary to use a combination of morphological characters and genetic data from both nuclear and chloroplast markers. © 2015 Fogelqvist et al.

Osipova S.,Siberian Institute of Plant Physiology and Biochemistry | Permyakov A.,Siberian Institute of Plant Physiology and Biochemistry | Permyakova M.,Siberian Institute of Plant Physiology and Biochemistry | Davydov V.,Siberian Institute of Plant Physiology and Biochemistry | And 2 more authors.
Cereal Research Communications | Year: 2011

Variation in tolerance of prolonged drought was identified among a set of single chromosome bread wheat substitution lines, involving the replacement of each cv. Chinese Spring chromosome in turn with its homologue from a synthetic hexaploid (Triticum dicoccoides × Aegilops tauschii). Water stress was applied under controlled conditions by limiting the supply of water to 30% from 100% aqueous soil. The reaction to the resulting long-term drought stress was quantified by three indices, based on grain yield components. Enhanced drought tolerance was associated with the presence of donor chromosomes 1A, 5A, 1D, 3D, 5D and 6D, and enhanced susceptibility with chromosomes 3A, 4B and 7D. © 2011 American Physical Society.

Pyatrikas D.V.,Siberian Institute of Plant Physiology and Biochemistry | Fedoseeva I.V.,Siberian Institute of Plant Physiology and Biochemistry | Varakina N.N.,Siberian Institute of Plant Physiology and Biochemistry | Rusaleva T.M.,Siberian Institute of Plant Physiology and Biochemistry | And 4 more authors.
FEMS Microbiology Letters | Year: 2015

Moderate heat shock increased reactive oxygen species (ROS) production that led to cell death in glucose-grown Saccharomyces cerevisiae cells. Conditions that disturb mitochondrial functions such as treatment by uncouplers and petite mutation were shown to inhibit ROS production and protects cell from thermal death. Hence, mitochondria are responsible for ROS production and play an active role in cell death. An increase in ROS production was accompanied by hyperpolarization of inner mitochondrial membrane. All agents suppressing hyperpolarization also suppressed heat-induced ROS production. It was supposed that generation of ROS under moderate heat shock in glucose-grown S. cerevisiae cells is driven by the mitochondrial membrane potential. © FEMS 2015. All rights reserved.

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