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Rashmi B.S.,Rashtreeya Vidyalaya College of Engineering | Liny P.,Shridevi institute of Engineering and technology
International Journal of Pharma and Bio Sciences | Year: 2013

Different fungal species, Aspergillus niger, Aspergillus flavus and Pencillium notatum selected for the production of extracellular fibrinolytic enzymes, Production rate was enhanced by using different carbon source where maltose increased the production rate of enzymes 2.4mg/ml in Aspergillus niger, 2.2mg/ml Aspergillus flavus and glucose 2.2mg/ml in Pencillium notatum. Enzyme was fractionated using ammonium sulfate fractionation, different characteristic studies like protease activity showed 1.4units/mg of proteins in Aspergillus niger, 1.0 units/mg of proteins in Aspergillus flavus, 1.3units/mg of proteins in Penicillium notatum. Fibrinplate method showed 200 μg/ml, 600 μg/ml, 240 μg/ml in Aspergillus niger, Aspergillus flavus, Penicillium notatum respectively and anticoagulation clotting time assay showed 25, 20, 15 min delay in clotting activity in Aspergillus niger, Penicillium notatum, Aspergillus flavus respectively. The Protein bands found on SDS-PAGE for fibrinolytic enzymes from different fungal sp were approximately 14kD from Penicillium notatum, 27kD from Aspergillus niger, 26kD from Aspergillus flavus. The current research showed fibrinolytic enzyme enhancement in maltose and glucose and its characterization specified that be a novel method for yield enhancement.


Basavegowda N.,Shridevi Institute of Engineering and Technology | Sobczak-Kupiec A.,Cracow University of Technology | Malina D.,Cracow University of Technology | Yathirajan H.S.,University of Mysore | And 4 more authors.
Advanced Materials Letters | Year: 2013

Plant mediated synthesis of metallic nanoparticles is an increasing commercial demand due to the wide applicability in various areas such as electronics, catalysis, chemistry, energy, cosmetics and medicine. In the present investigation, synthesis of gold nanoparticles is done by using fruit extracts of Ananas comosus (L.). Nanoparticles were characterized by using UV visible absorption spectra. Their morphology, elemental composition and crystalline phase were determined by scanning electron microscopy, energy dispersive X-ray spectroscopy and selected area electron diffraction. FT-IR analysis was used to confirm the presence of gold nanoparticles in the extracts. The synthesized gold nanoparticles were generally found to be effective as antimicrobial agents against some important human pathogens like E.coli and Streptobacillus sp. which are affecting and cause diseases like food poisoning and rat-bite fever to human beings respectively. © 2013 VBRI press.


Sadananda T.S.,Shridevi Institute of Engineering and Technology | Nirupama R.,Shridevi Institute of Engineering and Technology | Chaithra K.,Shridevi Institute of Engineering and Technology | Govindappa M.,Shridevi Institute of Engineering and Technology | And 2 more authors.
Journal of Medicinal Plants Research | Year: 2011

Thirteen different endophytic fungi were isolated from different parts of Tabebuia argentea. These endophytic fungal extracts were prepared, using ethyl acetate and evaluated for their phytochemical constituents. Aspergillus niger and Alternaria alternata yielded saponins, phenolic compounds, anthraquinones, steroids, cardiac glycosides and tannins. Other endophytes yielded less phytochemical compounds compared to plant extracts. Naphthoquinone (natural lapachol) was identified in A. niger and A. alternata. These two endophytes also exhibited significant antimicrobial activity against an array of pathogenic fungi and bacteria. Endophytic isolates of A. niger and A. alternata are of particular interest because they showed significant antagonistic activity against all tested bacteria and fungi at different range. The total antioxidant capacity and phenolic content of the fungal cultures ranged from 4299 to 5276 μmol/L and from 2.5 to 2.6 mg gallic acid/100 mL culture respectively. The fungal culture, endophytes, A. niger and A. alternata showed strongest antioxidant capacity, having the highest levels of phenolics. This is the first report of lapachol (naphthoquinone) producing endophytes and their antimicrobial and antioxidant activities. This investigation reveals that the metabolites produced by a variety of endophytic fungi can be a potential source of novel natural antimicrobials, antioxidants and anticancer agents. © 2011 Academic Journals.


Govindappa M.,Shridevi Institute of Engineering and Technology | Sadananda T.S.,Shridevi Institute of Engineering and Technology | Channabasava R.,Shridevi Institute of Engineering and Technology | Raghavendra V.B.,Teresian Center
International Journal of Pharma and Bio Sciences | Year: 2011

The ethanol, methanol and water extracts of Tecoma stans effective against tested for antiinflammatory activity, lipoxygenase, xanthine oxidase and acetycholinesterase inhibitory activity. The methanol, ethanol and water extracts showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation is 86.32, 83.81, 61.39 g/ml and red blood cells membrane stabilization with 71.29, 67.78, 54.48 g/ml respectively. Proteinase activity was also significantly inhibited by the methanol (83.91), ethanol (81.88) and water (61.73). In addition, the methanol and ethanol extracts showed anti-lipoxygenase activity range from 39.32-58.92. The study also showed that methanol and ethanol fractions exhibited a moderate xanthine oxidase inhibitory activity comparatively to the acetylcholinestearse which was weakly inhibited by the tested extracts. The results obtained in the present investigation indicate that methanol and ethanol extracts of T. stans can be a potential source of antiinflammatory agents.


Nagaraj B.,Shridevi Institute of Engineering and Technology | Krishnamurthy N.B.,Shridevi Institute of Engineering and Technology | Liny P.,Shridevi Institute of Engineering and Technology | Divya T.K.,Shridevi Institute of Engineering and Technology | Dinesh R.,International Advanced Research Center for Powder Metallurgy And New Materials
International Journal of Pharma and Bio Sciences | Year: 2011

The synthesis of eco-friendly nanoparticles is evergreen branch of nanoscience for biomedical application. Low cost of synthesis and non toxicity are the main features which make it more attractive potential option for biomedical field. Here, we report the synthesis of gold nanoparticles in aqueous medium using flower extracts of Ixora coccinea (Chetty flower) as reducing and stabilizing agent. On treating chloroauric acid solution with extract, rapid reduction of chloroaurate ions is observed leading to the formation of the highly stable gold nanoparticles in solution. The synthesized nanoparticles are confirmed by color changes and it has been characterized by UV-visible spectroscopy. The UV- visible spectra indicate a strong Plasmon resonance that is located at ~550 nm. Presence of this strong broad plasmon peak has been well documented for various Me- NPs, with sizes ranging all the way from 2 to 100 nm. The morphology and size of the biologically synthesized gold nanoparticles were determined using TEM. The images clearly show that the average size of the nanotriangles is about 200 nm, while, the spherical like particles show very small size about 5-10 nm. The study also shows that gold nanoparticles with antibiotic show more inhibitory zones than compared to the standard antibiotics.


Krishnamurthy N.B.,Shridevi Institute of Engineering and Technology | Nagaraj B.,Shridevi Institute of Engineering and Technology | Malakar B.,Shridevi Institute of Engineering and Technology | Liny P.,Shridevi Institute of Engineering and Technology | Dinesh R.,International Advanced Research Center for Powder Metallurgy And New Materials
International Journal of Pharma and Bio Sciences | Year: 2012

An environment friendly technique for green synthesis of gold nanoparticles has been developed using the flower extract of Tagetes Erecta as reducing agent for reduction of Au3+ in aqueous solution. The nanoparticles obtained were characterized by UV-visible spectroscopy. The UV- visible spectra indicate a strong Plasmon resonance that is located at ~500 nm. The morphology and size of the biologically synthesized gold nanoparticles were determined using TEM. The images clearly show that the average size of the nanotriangles is about 200 nm, while, the spherical like particles show very small size about 8-10 nm. The antimicrobial activities of obtained gold particles have been studied with antibiotic, which show more inhibitory zones than the standard antibiotics.


Panduranga Murthy G.,Shridevi Institute of Engineering and Technology | Mamtharani D.R.,Shridevi Institute of Engineering and Technology | Tejas T.S.,Shridevi Institute of Engineering and Technology | Suarlikerimath N.M.,Shridevi Institute of Engineering and Technology
International Journal of Pharma and Bio Sciences | Year: 2011

The white bulbs of 'Wild Onion, Urginea indica' samples were collected from the hillocks near Vaddagere village of Tumkur district, a southern part of Karnataka. The study was performed extensively for the first time, leading to the evaluation of active constituents from wild onion bulb. The extract was prepared with Methanol and subjected for preliminary phyto-chemical and physicochemical analysis. The total ash content, acid insoluble and water soluble ash content were evaluated along with the fluorescence characteristics of the methanolic extract of Wild onion sps. The presence of primary and secondary metabolites such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. The extract was found to possess anti-bacterial activity in E. coli, S. aureus and P. aeruginosa isolated from infected patients. The Minimum inhibitory concentration (MIC) was also evaluated by 'Tube dilution' method and the result was found to be considerably effective against selected pathogenic bacteria. Such an effect might contribute to explaining the traditional use of wild onion sps, Urginea indica in the treatment of wound healing. The antioxidant activity was estimated by using DPPH free radical scavenging assay and the activity was increased with increase in concentration of methanolic fraction of wild Onion sps. The fractions of wild onion sps are free radical scavengers and are able to react with the DPPH radical, which might be attributed to their electron donating ability and suggested that antioxidant components in this Wild Onion sps was capable of reducing oxidants and scavenging free radicals. This also indicates that, tubers of wild onion, Urginea indica' are of therapeutic potential due to their high freeradical scavenging activity. The presence of high amount of saponins justifies the practice of treatment for disturbances in the gastrointestinal tract by Traditional healers. The role of phyto-chemical constituents of this Wild Onion, Urginea indica sps in traditional medicine treatment is discussed. Hence, the formulation of extract of Urginea indica' needs to be purified using biophysical techniques towards development of a potential drug/ lead molecule against microbial infection, inflammation and wound healing respectively.


Bhuvaneshwari N.,Shridevi Institute of Engineering and Technology | Aryaputra A.,Shridevi Institute of Engineering and Technology
2013 IEEE 2nd Global Conference on Consumer Electronics, GCCE 2013 | Year: 2013

Technology innovation fast-forwards natural selection in the telecommunications marketplace by destroying old markets and creating new ones in their place. IPTV usually means distribution of television or video content over a controlled IP network, where the end consumer receives the information through a set-top box which is connected to its normal broadband connection. Just because its name is IPTV (Internet Protocol Television) it does not mean that information is sent over the internet, only that IP protocol is used. So we should not consider streaming video over the internet as IPTV. Many of the world's major telecommunications providers are exploring IPTV as a new revenue opportunity from their existing markets and as a defensive measure against encroachment from more conventional Cable Television services. Also, there is a growing number of IPTV installations within schools, universities, corporations and local institutions. Will the content of IPTV be different from that of the traditional video delivery systems of broadcast television, video recordings, cable television and satellite television? How are the new IPTV business structured? What new modes of intermediation are replacing older forms in this market? Are things moving away from a mass audience model for high end television toward a more niche oriented approach? These are some of the questions that we have tried to answer in this paper. We have also given an insight to the hope of IPTV in Indian market. © 2013 IEEE.


Santoyo G.,Universidad Michoacana de San Nicolás de Hidalgo | del Orozco-Mosqueda M.C.,Universidad Michoacana de San Nicolás de Hidalgo | Govindappa M.,Shridevi Institute of Engineering and Technology
Biocontrol Science and Technology | Year: 2012

Plant pathogens are responsible for many crop plant diseases, resulting in economic losses. The use of bacterial agents is an excellent option to fight against plant pathogens and an excellent alternative to the use of chemicals, which are offensive to the environment and to human health. Two of the most common biocontrol agents are members of the Bacillus and Pseudomonas genera. Both bacterial genera have important traits such as plant growth-promoting (PGP) properties. This review analyzes pioneering and recent works and the mechanisms used by Bacillus and Pseudomonas in their behaviour as biocontrol and PGP agents, discussing their mode of action by comparing the two genera. Undoubtedly, future integrated research strategies for biocontrol and PGP will require the help of known and novel species of both genera. © 2012 Copyright Taylor and Francis Group, LLC.


Geetha D.D.,Bangalore University | Nalini N.,Shridevi Institute of Engineering and Technology | Biradar R.C.,Bangalore University
AI Communications | Year: 2015

Wireless Sensor Networks (WSNs) have been extensively used in various applications such as environmental monitoring, industrial monitoring, agriculture, green house monitoring, structural monitoring, passive localization, tracking and battlefield surveillance. Sensor nodes in these applications are required to sense and process the physical conditions like temperature, pressure, humidity, rainfall, fog, etc. and route the data to a predefined base station or a sink node. In most of these applications, sensor nodes are deployed in public domain and they are prone to be attacked by many types of attacks where in the data confidentiality, integrity and authentication are compromised. Some times, it is difficult to correctly locate the compromised data unless we use autonomous third party that uses intelligent software techniques to safeguard our data and correctly means route it to destined party. In this paper, we propose a Trust based Neighbor Identification in Wireless Sensor Networks (TNIWSN) using agents to identify trustworthy nodes in a network. The trusted neighbor identification is necessary for routing the data through trustworthy neighbors and avoid untrusted neighbors that are compromised by various threats. The proposed scheme operates in following phases. (1) Defining safeguard agency that consists of one static agent known as Safeguard Manager Agent (SMA) and one mobile agent known as Trusted Neighbor Agent (TNA) and a knowledge base. (2) Safeguard agency identifies trustworthy neighbor nodes using static and mobile agents by means of trust model that comprise of the probability model and Message Authentication Code (MAC) model. The probability model identifies trusted neighbors based upon the probabilities of trustworthiness of wireless channel and the trustworthiness of sensor node.MAC model encrypts the message using the two keys k1 and k2 are generated with k-ERF (Error Resilient Function) key generation process to ensure the trustworthiness of neighbors identified by the probability model. (3) MACs are dynamically computed by agents (either on sender node or on neighbor node) by generating keys with the help of k-ERF. (4) Agents effectively identify possible security threats on wireless channel and node. Simulation analysis shows that TNIWSN outperforms Neighbor based Malicious Node Detection (NMND) in Wireless Sensor Networks in terms of average success ratio and memory overhead. © 2015 IOS Press and the authors.

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