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Ali S.S.,Shri Shivaji Science College Amravati | Vidhale N.N.,Shri Shivaji Science College Amravati
Journal of Pure and Applied Microbiology | Year: 2012

In the present study thirty six pseudomonas species were isolated and identified as Pseudomonas fluorescence from rhizospheric soil of different crops such as sugarcane, groundnut and soybean in different places of Parbhani District. All isolates were shown siderophore production on succinate medium. The maximum siderophore production was found in PFSP03 strain i.e 72%. The strain produced both hydroxamate and chatacholate type of siderophore which was maximum at 10mM iron concentration in the medium. Also antifungal activity was observed against phytopathogens viz., Alternaria alternata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Macrophomina phaseolina. Source

Ali S.S.,Shri Shivaji Science College Amravati | Vidhale N.N.,Shri Shivaji Science College Amravati
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

Pseudomonas aeruginosa is an opportunistic pathogen and a leading causes of hospital-aquired pneumonia. In present study 50 Pseudomonas strains was isolated from patients and hospital environment and were identified Pseudomonas aeruginosa. All isolates were studied for siderophore production on different medium at different pH and iron concentration also they were studied for antifungal activity. The result indicated that all 50 isolates showed siderophore production on CAS agar plate. Among the 50 isolates PAUTI4 strain showed maximum siderophore production on succinate medium (61mg/L) without addition of iron at optimum pH 7.0. The cell free supernatant of PAUT14 showed growth inhibition of Aspergillus flavus, A. fumigates, A.niger and Candida albicans. Maximum growth inhibition was found with Aspergillus flavus (67%). Hence Pseudomonas aeruginosa can be found efficient for siderophore production and biocontrol for fungal pathogen. Source

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