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Patel H.U.,Shri Sarvajanik Pharmacy College | Patel C.N.,Shri Sarvajanik Pharmacy College
Journal of AOAC International | Year: 2010

A simple, precise, and accurate isocratic RP-HPLC method was developed and validated for determination of eprosartan in bulk drug and tablets. Isocratic RP-HPLC separation was achieved on a Phenomenex C 18 column (250 × 4.6 mm id, 5 μm particle size) using the mobile phase 0.5% formic acid-methanol-acetonitrile (80 + 25 + 20, v/v/v, pH 2.80) at a flow rate of 1.0 mL/min. The retention time of eprosartan was 7.64 ± 0.05 min. The detection was performed at 232 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability, and specificity. The method was linear in the concentration range of 10-400 μg/mL with a correlation coefficient of 0.9999. The repeatability for six samples was 0.253% RSD; the intraday and interday precision were 0.21-0.57 and 0.33-0.71% RSD, respectively. The accuracy (recovery) was found to be in the range of 99.86-100.92%. The drug was subjected to the stress conditions hydrolysis, oxidation, photolysis, and heat. Degradation products produced as a result of the stress conditions did not interfere with detection of eprosartan; therefore, the proposed method can be considered stability-indicating.


Patel B.N.,Shri Sarvajanik Pharmacy College | Patel C.N.,Shri Sarvajanik Pharmacy College
Journal of Planar Chromatography - Modern TLC | Year: 2011

A quantitative high-performance thin-layer chromatography (HPTLC) method for determination of darunavir ethanolate (DRV) in tablets has been established and validated. DRV from the formulations was separated and identified on silica gel 60 F 254 HPTLC plates with toluene-ethyl acetate-methanol 7.0:2.0:1.0 (ν/ν) as mobile phase. The plates were developed to a distance of 8 cm. Quantification was performed at λ = 267 nm. Well-resolved bands were obtained for DRV. The method was validated for specificity, precision, robustness, and accuracy. The calibration plot for DRV standard was linear in the range 250-1750 ng per band with r = 0.9994, slope = 0.4253, and intercept = 44.81. The limits of detection and quantification were 15.28 and 45.84 ng per band, respectively. The method is selective, sensitive, and specific, with potential application in pharmaceutical analysis. © Akadémiai Kiadó, Budapest.


Prajapati S.,Shri Sarvajanik Pharmacy College | Patel L.,C U Shah College Institute Of Pharmacy And Research | Patel C.,C U Shah College Institute Of Pharmacy And Research
Iranian Journal of Pharmaceutical Research | Year: 2011

The purpose of this research was to prepare a floating matrix tablet containing domperidone as a model drug. Polyethylene oxide (PEO) and hydroxypropyl methylcellulose (HPMC) were evaluated for matrix-forming properties. A simplex lattice design was applied to systemically optimize the drug release profile. The amounts of PEO WSR 303, HPMC K15M and sodium bicarbonate were selected as independent variables and floating lag time, time required to release 50% of drug (t 50) and 80% of drug (t 80), diffusion coefficient (n) and release rate (k) as dependent variables. The amount of PEO and HPMC both had significant influence on the dependent variables. It was found that the content of PEO had dominating role as drug release controlling factor, but using suitable concentration of sodium bicarbonate, one can tailor the desired drug release from hydrophilic matrixes. The linear regression analysis and model fitting showed that all these formulations followed Korsmeyer and Peppas model, which had a higher value of correlation coefficient (r). The tablets of promising formulation were found to be stable for 3 months under accelerated (40°C/75% RH) stability testing. © 2011 by School of Pharmacy.


Shah H.J.,Torrent Pharmaceutical Ltd. | Shah H.J.,Shri Sarvajanik Pharmacy College | Subbaiah G.,Torrent Pharmaceutical Ltd. | Patel D.M.,Torrent Pharmaceutical Ltd. | And 2 more authors.
Journal of Chromatographic Science | Year: 2010

A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) method has been developed and validated for the determination of lamotrigine in human plasma using multiplexing technique (two HPLC units connected to one MS-MS). Lamotrigine was extracted from human plasma by solidphase extraction technique using Oasis Hydrophilic Lipophilic Balance (HLB) or N-vinylpyrrolidone and divinylbenzene cartridge. A structural analog, 3, 5-diamino-6-phenyl-1, 2, 4-triazine, was used as an internal standard (IS). A BetaBasic C8 column was used for the chromatographic separation of analytes. The mass transition [M+H]+ ions used for detection were m/z 256.0 → 211.0 for lamotrigine and m/z 188.0 → 143.0 for IS. The method involved a simple multiplexing, rapid solid-phase extraction without evaporation and reconstitution. The proposed method has been validated for a linear range of 0.025 to 10.000 μg/mL with a correlation coefficient ≥ 0.9991. The limit of quantification for lamotrigine was 0.025 μg/mL, and limit of detection was 50.000 pg/mL. The intra-run and inter-run precision and accuracy were within 10.0% for intra-HPLC runs and inter-HPLC runs. The overall recoveries for lamotrigine and IS were 97.9% and 92.5%, respectively. Total MS run time was 1.4 min per sample. The validated method has been successfully used to analyze human plasma samples for applications in pharmacokinetic, bioavailability, bioequivalence, or in vitro in vivo correlation studies.


Soni J.P.,Shri Sarvajanik Pharmacy College | Sen D.Y.,Shri Sarvajanik Pharmacy College | Modh K.K.,Shri Sarvajanik Pharmacy College
Journal of Applied Pharmaceutical Science | Year: 2011

In the present investigation, a series of some synthesis and biological evaluation of some pyrazolone derivatives with imidazole, benzimidazole and benztriazole moiety were synthesized and tested for their anti-inflammatory activity in-vitro using celecoxib as a reference drug. Compound 8d was found to be the most potent derivative of the series with 75 %inhibition of inflammation.


Patel G.H.,Shri Sarvajanik Pharmacy College | Prajapati S.T.,Shri Sarvajanik Pharmacy College | Patel C.N.,Shri Sarvajanik Pharmacy College
Research Journal of Pharmacy and Technology | Year: 2011

This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of cinitapride and pantoprazole in pharmaceutical dosage form. Chromatographic separation of the drugs was performed on aluminium plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of ethyl acetate: methanol 9: 1(v/v). Densitometric evaluation of the separated zones was performed at 278 nm. The two drugs were satisfactorily resolved with Rf values 0.43±0.00 and 0.62±0.01 for cinitapride and pantoprazole respectively. The accuracy and reliability of the method was assessed by evaluation of linearity 100-700 ng/spot for cinitapride and 1000-7000 ng/spot for pantoprazole, precision (intra-day RSD 0.05-0.40% and inter-day RSD 0.13-0.39% for cinitapride, and intra-day RSD 0.10-0.20% and inter-day RSD 0.13-0.60% for pantoprazole), accuracy (98.6 - 100.4% for cinitapride and 98.2 - 101.5% for pantoprazole), and specificity (100.40 ± 1.01% for cinitapride and 99.19 ± 0.99% for pantoprazole), in accordance with ICH guidelines. This method was successfully applied to the determination of these drugs in pharmaceutical dosage forms. © RJPT All right reserved.


Kapadia K.B.,Shri Sarvajanik Pharmacy College | Bhatt P.A.,JKK Nataraja Dental College and Hospital | Shah J.S.,Shri Sarvajanik Pharmacy College
Journal of Pharmacology and Pharmacotherapeutics | Year: 2012

Objective: To determine the association between altered thyroid hormones and insulin resistance (IR). Materials and Methods: Eight euthyroid (EU), eight hypothyroid (HO), and eight hyperthyroid (HR) patients with no past medical history were studied in this cross-sectional study at the Care Institute of Medical Sciences, Ahmedabad, India, The fasting blood sample were analyzed for thyroid stimulating hormone (TSH), lipid profile, insulin, and glucose. Homeostatic model assessment (HOMA) was calculated for assessing IR. Results: HOMA values were significantly higher in HR and HO groups as compared to the EU group (P < 0.05). Insulin levels were also found to be significantly increased in HR and HO groups as compared to the EU group (P < 0.05). Cholesterol, triglycerides (TG), and low density lipoprotein (LDL) were significantly raised in HO as compared to EU and HR groups (P < 0.05) whereas high density lipoprotein levels (HDL) were lower. HOMA and insulin were found to be positively correlated with TSH in HO and negatively in HR. Conclusion: Thyroid disorder, including both hypo- and hyper have been associated with IR due to various mechanisms such as altered insulin secretion and lipid levels. IR was comparable in patients with both HO and HR. Although HO and HR constitute an IR state, more studies need to be done in order to clarify the underlying pathogenic mechanism. Thus, an altered thyroid state can lead to IR leading to glucose-related disorder such as diabetes dyslipidemia.


Patel B.,Shri Sarvajanik Pharmacy College | Prajapati P.,Shri Sarvajanik Pharmacy College | Patel C.,Shri Sarvajanik Pharmacy College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

In the present investigation mucoadhesive oral controlled release tablets of indomethacin were formulated for prolong anti inflammatory and antipyretics with analgesic action. Matrix tablets of indomethacin were formulated using two mucoadhesive polymers namely carbopol 934 LR and hydroxyl propyl cellulose. Solubility of Indomethacin was increased by solid dispersion using PEG 6000. Two layered tablets formulation, designed with an immediately releasing layer containing loading dose (25 mg Indomethacin) using a super disintegrate Ac-Di-Sol and a sustain releasing layer (75 mg Indomethacin) using Carbopol 934 LR or hydroxyl propyl cellulose. Carbopol 934 LR mucoadhesive polymers gave better controlled drug release compare to hydroxyl propyl cellulose and mixture of Carbopol 934 LR and Hydroxyl propyl cellulose as 1:1 in mucoadhesive controlled release oral bilayer tablets of indomethacin. © 2010 RJPBCS.


Patel G.H.,Shri Sarvajanik Pharmacy College | Prajapti S.T.,Shri Sarvajanik Pharmacy College | Patel C.N.,Shri Sarvajanik Pharmacy College
International Journal of Pharmacy and Technology | Year: 2012

A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Cinitapride and Pantoprazole. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5μ, 250 mm x 4.6 mm] utilizing a mobile phase of acetonitrile: phosphate buffer (80:20 v/v, pH 6.8) at a flow rate of 1.0 ml/min with UV detection at 278 nm. The retention time of Cinitapride and Pantoprazole was 3.18 min and 4.725 min respectively. The method is accurate (98.22-101.66% and 98.5- 101.40% for cinitapride and pantoprazole respectively), precise (method precision 0.44% and intermediate precision 0.78%) and linear within range 1.5-12μg/ml and 20-160 μg/ml for cinitapride and pantoprazole respectively. The correlation coefficient was found to be r 2=0.9991 for both the drugs. The detection limit of Cinitapride and Pantoprazole was 0.064 μg/ml and 0.78 μg/ml while quantification limit was 0.205 μg/ml and 2.38 μg/ml respectively. The proposed method is applicable to routine analysis for simultaneous estimation of Cinitapride and Pantoprazole in pharmaceutical dosage form.


Chavda H.V.,Shri Sarvajanik Pharmacy College | Patel C.N.,Shri Sarvajanik Pharmacy College
Trends in Biomaterials and Artificial Organs | Year: 2010

The synthesis of superporous hydrogel composites (SPHCs) with carboxylmethylcellulose sodium (NaCMC) as a composite material was carried out by solution polymerization. The characterization studies were performed by measurement of apparent density, swelling studies, mechanical strength studies, and scanning electron microscopy (SEM). In double distilled water SPHCs showed tremendous increase in equilibrium swelling capacity. But, when SPHCs were placed in simulated gastric fluid showed less equilibrium swelling capacity. SPHCs showed improved penetration pressure as the NaCMC concentration increased. SEM images clearly indicate the formation of interconnected pore, capillary channels, and the adherence of NaCMC molecules around the periphery of pores. © Society for Biomaterials and Artificial Organs (India), 2010.

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