Shri Rawatpura Sarkar Institute of Pharmacy

Kumhāri, India

Shri Rawatpura Sarkar Institute of Pharmacy

Kumhāri, India
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Gidwani B.,University Institute of Pharmacy | Gidwani B.,Shri Rawatpura Sarkar Institute of Pharmacy | Vyas A.,University Institute of Pharmacy | Vyas A.,Shri Rawatpura Sarkar Institute of Pharmacy | Vyas A.,University of Minnesota
International Journal of Biological Macromolecules | Year: 2017

Altretamine is a synthetic drug approved for treatment of ovarian cancer. The only drawback with its formulation is poor aqueous solubility and low oral bioavailability. In the present work an attempt has been made to prepare inclusion complex of altretamine with epichlorohydrin beta cyclodextrin. The complexes were prepared by kneading, co-evaporation and freeze-drying method and were confirmed by FTIR, XRD, DSC, drug content and dissolution study. Kneaded complex possess maximum solubilizing efficiency of 82.63 in 25 mM Epi-β-CD solution. SLNs of pure altretamine and ALT complexed with Epi-β-CD were prepared by modified emulsification–ultrasonication method. The particle size and zeta potential was found to be 151.5 nm and −21.3 mV. The drug release pattern of SLNs was bi-phasic in nature; with an initial burst release followed by sustained drug release. Pharmacokinetic study showed that the average Cmax was found to be 0.94 μg/ml, which was 2.47 times higher as compared to the pure drug. The AUCt for SLNs was 150 min μg h/ml and 54 min μg h/ml for pure ALT suspension which proved that the SLNs exhibited greater absorption compared to the pure drug. Thus, smaller particle size, higher entrapment efficiency and enhanced aqueous solubility led to improvement in oral bioavailability of ALT. © 2017 Elsevier B.V.


Asirvatham R.,Shri Rawatpura Sarkar Institute of Pharmacy | Christina A.J.M.,AIMST University
Advanced Pharmaceutical Bulletin | Year: 2013

Purpose: The aim of present in vitro studies was performed to examine the antioxidant and anticancer activities of ethanol and aqueous extracts of Drosera indica L. Methods: Different concentrations (5 - 640mcg/ml) of the ethanol (EEDI) and aqueous (AEDI) extracts of D.indica L were used in various antioxidant assay methods such as hydroxyl radicals, DPPH, super oxide radical scavenging activity, chelating ability of ferrous ion, nitric oxide radical inhibition, ABTS and reducing power. Ascorbic acid (AA) was used as the standard antioxidant for the free radical scavenging assays. Dalton's Ascitic Lymphoma (DAL) and Ehrlich Ascitic Carcinoma (EAC) cell lines were used as the in vitro cancer models for the tryphan blue dye and LDH leakage assays, where 5 to 250mcg /ml of both EEDI and AEDI were tested. Results: EEDI showed antioxidant activities with the minimum IC50 values of 34.8±0.43 mcg/ml in scavenging of hydroxyl radical and moreover AEDI showed minimum IC50 values of 94.51±0.84 mcg/ml in Fe2+chelating assay. EEDI on the reducing power assay and ABTS showed higher IC50 than standard AA. IC50 values of AEDI on Fe2+ chelating assay and super oxide radical assay was lesser than IC50 value of AA. Both extracts at 250mcg/ml dose showed remarkable increase in the percentage of dead cancer cells (90% by EEDI and 86% by AEDI in DAL model and 89% by EEDI and 80% by AEDI in EAC model). Conclusion: It is concluded from this study that D.indica L exhibited excellent antioxidant activity against the different in vitro antioxidant models and anticancer activity against the two different cell lines tested. © 2013 by Tabriz University of Medical Sciences.


Kaur C.D.,Shri Rawatpura Sarkar Institute of Pharmacy | Kaur C.D.,Pandit Ravishankar Shukla University | Saraf S.,Pandit Ravishankar Shukla University | Saraf S.,Guru Ghasidas University
International Journal of Drug Delivery | Year: 2011

Botanical photochemopreventives are used to fight against the adverse effects of ultra violet rays on skin which leads to photoaging. Phytoconstituents present in Punica granatum extract have antioxidant, anti-carcinogenic and photoprotective properties which was proved by estimation of phenolic content, sun protection factor and antioxidant effect determination of the alcoholic extract. Extract loaded nanotransfersomes were prepared, evaluated for size, entrapment efficiency and incorporated into the cream. The presence of vesicles in the cream was confirmed by the transmission electron microscopy and the efficacy of the formulated creams by the physicochemical parameters. The nanotransfersomal and conventional cream formulations were in vivo tested for change in skin hydration (Corneometer), viscoelasticity (Cutometer) and sebum content (Sebumeter). The photoprotective effect of formulations was further confirmed by biochemical studies. Improved skin properties (viscoelasticity, skin hydration, sebum content) and biochemical parameters (superoxide dismutase, catalase, total protein and ascorbic acid level) with decreased malondialdehyde level was observed with nanotransfersomal cream formulations as compared to conventional creams. The efficacy was in the order nanotransfersomal creams > Conventional creams> Empty nanotransfersomal cream > Base cream.


PubMed | Shri Rawatpura Sarkar Institute of Pharmacy
Type: Journal Article | Journal: International journal of biological macromolecules | Year: 2016

The effect of Ca


PubMed | Shri Rawatpura Sarkar Institute of Pharmacy
Type: | Journal: International journal of biological macromolecules | Year: 2016

In the present work, an unreported graft copolymer of carboxymethyl xanthan gum and acrylamide has been synthesised by free radical polymerisation in a nitrogen atmosphere using ammonium persulphate as an initiator. The optimum reaction conditions adopted for affording maximum percentage of grafting including its grafting efficiency were obtained by varying the concentration of carboxymethyl xanthan gum from 4 to 24 g dm(-3); ammonium persulphate from 510(-4) to 3010(-4)mol dm(-3); acrylamide from 0.4 to 1.2 mol dm(-3); reaction temperature from 55 to 75C and reaction time from 30 to 90 min. The synthesised graft copolymer has been characterised by (1)H NMR, FTIR spectroscopy, X-ray diffraction measurement, thermal analysis, viscosity measurement and scanning electron microscopy. However, grafting of acrylamide onto carboxymethyl xanthan gum backbone enhanced its thermal stability. This graft copolymer might be well exploited globally as a potential carrier for drug delivery system.


Sahu R.,Shri Rawatpura Sarkar Institute of Pharmacy | Tiwari S.,Shri Rawatpura Sarkar Institute of Pharmacy | Kalyani G.,Shri Rawatpura Sarkar Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Substituted salicylic acid and thiosemicarbazide were refluxed in acidic medium to obtain 2-amino-5-(o-hydroxysubstituted phenyl)-1, 3, 4-thiadiazol (1) which on treatment with various aryl aldehydes and then with thioglycolic acid gives 2-(substitutedphenyl)-3-(2-hydroxysubstitutedphenyl-1, 3, 4-thiadiazol-2yl) thiazolidin-4-ones (3). Structure of the compounds (3a-l) were confirmed on the basis of IR and 1H NMR data. All the compounds were tested against Staphylococcus areus, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger.


Anand K.,Sarguja University | Pranita K.,Shri Rawatpura Sarkar Institute of Pharmacy
International Journal of Pharmacognosy and Phytochemical Research | Year: 2013

The present study deals with the Pharmacognostical & Phytochemical study of the plant "Jussiaea suffruticosa" for its identification and to distinguish it from the co-existing weeds and adulteration. In the present investigation, microscopical characters are evaluated and different parameters are applied for the physico-chemical studies include evaluation of colour, consistency of different extracts, extractive value, ash value, moisture content, fluorescence analysis and also qualitative phytochemial screening was performed. It was earlier reported as an astringent, carminative, laxative, diuretic and anthelmintic properties. Since there is no proper information regarding this plant, our efforts were devoted to study the pharmacognostical and phytochemical properties of this plant. Thus, present study revealed the plant extract contains different chemical constituents like alkaloid, phenolic compound, saponin, flavonoid, proteins & amino acids.


Dubey S.,Shri Rawatpura Sarkar Institute of Pharmacy | Kashyap P.,Shri Rawatpura Sarkar Institute of Pharmacy | Pandey A.,Shri Rawatpura Sarkar Institute of Pharmacy | Thakur D.,Shri Rawatpura Sarkar Institute of Pharmacy
International Journal of PharmTech Research | Year: 2013

Pioglitazone is an oral hypoglycemic agent. It selectively stimulates the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ). Literature review revealed that 1st order derivative spectroscopic method is not available. The aim of this study is to develop a simple, accurate, precise and economical procedure for estimation of Pioglitazone by first order derivative spectroscopy. The method is based upon determination of D1 value of Pioglitazone at 231.5 nm, in 0.1N NaOH. Different analytical performance parameters such as linearity, range, system precision, robustness, sensitivity and accuracy were determined according to the ICH guidelines. Pioglitazone at its λ max shows linearity in the concentration range 15- 65μg/mL.


Pandey A.,Shri Rawatpura Sarkar Institute of Pharmacy | Sawarkar H.,Shri Rawatpura Sarkar Institute of Pharmacy | Singh M.,RCPSR | Kashyap P.,Shri Rawatpura Sarkar Institute of Pharmacy | Ghosh P.,Shri Rawatpura Sarkar Institute of Pharmacy
International Journal of ChemTech Research | Year: 2011

A simple, precise and accurate UV spectrophotometric method has been developed and validated for the estimation of Telmisartan in bulk and tablet dosage form. The zero order spectra of Telmisartan in 0.1N NaOH shows λmax at 234.0 nm and estimation was carried out by A(1% 1cm) and by comparison with standard. Calibration graph was found to be linear (r2 =0.999) over the concentration range of 4-24 μg/mL. The proposed method was validated for its accuracy, precision, specificity, ruggedness and robustness. The method can be adopted in its routine analysis.


Sahu S.,Shri Rawatpura Sarkar Institute of Pharmacy | Sahu S.,Pandit Ravishankar Shukla University | Saraf S.,Pandit Ravishankar Shukla University | Kaur C.D.,Shri Rawatpura Sarkar Institute of Pharmacy | Kaur C.D.,Pandit Ravishankar Shukla University
Pakistan Journal of Biological Sciences | Year: 2013

This study describes the release and retention of a herbal lipophilic drug in sustained and controlled manner in skin layers, given topically, intended for skin cancer. Quercetin -loaded nanoparticles were prepared by nanoprecipitation technique using ethylcellulose as polymer. Ethylcellulose was selected as it is biocompatible, but non-biodegradable and hence can act as a reservoir in skin furrows and ducts. It was observed that the Quercetin: Ethylcellulose: Tween 80 at different ratios affects particle sizes along with yield and entrapment efficiency. It was found that the size of nanoparticles could be varied by changing the speed of agitation and sonication. The nanoparticles were prepared in particle size range 228.77±2.0 nm and the zeta potential of the selected formulation were found to be -16.7 mV, which shows the stability of the preparation. The percent entrapment efficiency was found to be in the range from 51.96 to 53.93% and percent loading capacity in the range 34.19 to 5.12%. The amount of drug release from nanoparticles and of drug retained in skin was compared using ex vivo study which shows that the drug being lipophilic could be retained in the skin for longer duration thus reducing the dose and frequency of drug administration. Further the amount of drug reaching to other organs is also reduced since the systemic absorption of drug was low. Thus, Quercetin loaded nanoparticles were prepared for topical use. © 2013 Asian Network for Scientific Information.

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