Time filter

Source Type

Gidwani B.,University Institute of Pharmacy | Gidwani B.,Shri Rawatpura Sarkar Institute of Pharmacy | Vyas A.,University Institute of Pharmacy | Vyas A.,Shri Rawatpura Sarkar Institute of Pharmacy | Vyas A.,University of Minnesota
International Journal of Biological Macromolecules | Year: 2017

Altretamine is a synthetic drug approved for treatment of ovarian cancer. The only drawback with its formulation is poor aqueous solubility and low oral bioavailability. In the present work an attempt has been made to prepare inclusion complex of altretamine with epichlorohydrin beta cyclodextrin. The complexes were prepared by kneading, co-evaporation and freeze-drying method and were confirmed by FTIR, XRD, DSC, drug content and dissolution study. Kneaded complex possess maximum solubilizing efficiency of 82.63 in 25 mM Epi-β-CD solution. SLNs of pure altretamine and ALT complexed with Epi-β-CD were prepared by modified emulsification–ultrasonication method. The particle size and zeta potential was found to be 151.5 nm and −21.3 mV. The drug release pattern of SLNs was bi-phasic in nature; with an initial burst release followed by sustained drug release. Pharmacokinetic study showed that the average Cmax was found to be 0.94 μg/ml, which was 2.47 times higher as compared to the pure drug. The AUCt for SLNs was 150 min μg h/ml and 54 min μg h/ml for pure ALT suspension which proved that the SLNs exhibited greater absorption compared to the pure drug. Thus, smaller particle size, higher entrapment efficiency and enhanced aqueous solubility led to improvement in oral bioavailability of ALT. © 2017 Elsevier B.V.


PubMed | Shri Rawatpura Sarkar Institute of Pharmacy
Type: Journal Article | Journal: International journal of biological macromolecules | Year: 2016

The effect of Ca


PubMed | Shri Rawatpura Sarkar Institute of Pharmacy
Type: | Journal: International journal of biological macromolecules | Year: 2016

In the present work, an unreported graft copolymer of carboxymethyl xanthan gum and acrylamide has been synthesised by free radical polymerisation in a nitrogen atmosphere using ammonium persulphate as an initiator. The optimum reaction conditions adopted for affording maximum percentage of grafting including its grafting efficiency were obtained by varying the concentration of carboxymethyl xanthan gum from 4 to 24 g dm(-3); ammonium persulphate from 510(-4) to 3010(-4)mol dm(-3); acrylamide from 0.4 to 1.2 mol dm(-3); reaction temperature from 55 to 75C and reaction time from 30 to 90 min. The synthesised graft copolymer has been characterised by (1)H NMR, FTIR spectroscopy, X-ray diffraction measurement, thermal analysis, viscosity measurement and scanning electron microscopy. However, grafting of acrylamide onto carboxymethyl xanthan gum backbone enhanced its thermal stability. This graft copolymer might be well exploited globally as a potential carrier for drug delivery system.


Pandey A.,Shri Rawatpura Sarkar Institute of Pharmacy | Sawarkar H.,Shri Rawatpura Sarkar Institute of Pharmacy | Singh M.,RCPSR | Kashyap P.,Shri Rawatpura Sarkar Institute of Pharmacy | Ghosh P.,Shri Rawatpura Sarkar Institute of Pharmacy
International Journal of ChemTech Research | Year: 2011

A simple, precise and accurate UV spectrophotometric method has been developed and validated for the estimation of Telmisartan in bulk and tablet dosage form. The zero order spectra of Telmisartan in 0.1N NaOH shows λmax at 234.0 nm and estimation was carried out by A(1% 1cm) and by comparison with standard. Calibration graph was found to be linear (r2 =0.999) over the concentration range of 4-24 μg/mL. The proposed method was validated for its accuracy, precision, specificity, ruggedness and robustness. The method can be adopted in its routine analysis.


Gupta A.,Pandit Ravishankar Shukla University | Kaur C.D.,Pandit Ravishankar Shukla University | Kaur C.D.,Shri Rawatpura Sarkar Institute of Pharmacy | Saraf S.,Pandit Ravishankar Shukla University
Artificial Cells, Nanomedicine and Biotechnology | Year: 2016

The aim of this study was to design a targeted drug delivery system carrying a natural anticancer drug Quercetin (Qu), specifically for skin cancer. A central composite design was applied separately for each ligand, and the quadratic model was used for the process. The surface morphology was confirmed by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR), and in vitro release studies were also performed. The MTT assay was performed against two different cell lines, to measure their anticancer potentials and their targeting ability. The study thus reveals that MA-Qu-PLGA and FA-Qu-PLGA nanoparticles (NPs) can be used as effective drug delivery systems for skin cancer treatment encompassing natural drugs. © 2015 Informa Healthcare USA, Inc.


Sahu S.,Shri Rawatpura Sarkar Institute of Pharmacy | Sahu S.,Pandit Ravishankar Shukla University | Saraf S.,Pandit Ravishankar Shukla University | Kaur C.D.,Shri Rawatpura Sarkar Institute of Pharmacy | Kaur C.D.,Pandit Ravishankar Shukla University
Pakistan Journal of Biological Sciences | Year: 2013

This study describes the release and retention of a herbal lipophilic drug in sustained and controlled manner in skin layers, given topically, intended for skin cancer. Quercetin -loaded nanoparticles were prepared by nanoprecipitation technique using ethylcellulose as polymer. Ethylcellulose was selected as it is biocompatible, but non-biodegradable and hence can act as a reservoir in skin furrows and ducts. It was observed that the Quercetin: Ethylcellulose: Tween 80 at different ratios affects particle sizes along with yield and entrapment efficiency. It was found that the size of nanoparticles could be varied by changing the speed of agitation and sonication. The nanoparticles were prepared in particle size range 228.77±2.0 nm and the zeta potential of the selected formulation were found to be -16.7 mV, which shows the stability of the preparation. The percent entrapment efficiency was found to be in the range from 51.96 to 53.93% and percent loading capacity in the range 34.19 to 5.12%. The amount of drug release from nanoparticles and of drug retained in skin was compared using ex vivo study which shows that the drug being lipophilic could be retained in the skin for longer duration thus reducing the dose and frequency of drug administration. Further the amount of drug reaching to other organs is also reduced since the systemic absorption of drug was low. Thus, Quercetin loaded nanoparticles were prepared for topical use. © 2013 Asian Network for Scientific Information.


PubMed | Pandit Ravishankar Shukla University and Shri Rawatpura Sarkar Institute of Pharmacy
Type: Journal Article | Journal: Pakistan journal of biological sciences : PJBS | Year: 2014

This study describes the release and retention of a herbal lipophilic drug in sustained and controlled manner in skin layers, given topically, intended for skin cancer. Quercetin -loaded nanoparticles were prepared by nanoprecipitation technique using ethylcellulose as polymer. Ethylcellulose was selected as it is biocompatible, but non-biodegradable and hence can act as a reservoir in skin furrows and ducts. It was observed that the Quercetin: Ethylcellulose: Tween 80 at different ratios affects particle sizes along with yield and entrapment efficiency. It was found that the size of nanoparticles could be varied by changing the speed of agitation and sonication. The nanoparticles were prepared in particle size range 228.77 +/- 2.0 nm and the zeta potential of the selected formulation were found to be -16.7 mV, which shows the stability of the preparation. The percent entrapment efficiency was found to be in the range from 51.96 to 53.93% and percent loading capacity in the range 34.19 to 5.12%. The amount of drug release from nanoparticles and of drug retained in skin was compared using ex vivo study which shows that the drug being lipophilic could be retained in the skin for longer duration thus reducing the dose and frequency of drug administration. Further the amount of drug reaching to other organs is also reduced since the systemic absorption of drug was low. Thus, Quercetin loaded nanoparticles were prepared for topical use.


Dansena H.,Shri Rawatpura Sarkar Institute of Pharmacy | Hj D.,Shri Rawatpura Sarkar Institute of Pharmacy | Chandrakar K.,Shri Rawatpura Sarkar Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

The chemistry of pyrimidine derivatives plays an important role in the field of drugs, agriculture chemicals, and many biological processes. The chemistry of pyrimidine is a provoking field. In recent decades, a large number of pharmacological studies has been done on pyrimidine and their derivative. However, still more research is required in order to necessity the biological compounds. Numerous methods for the synthesis of pyrimidine and also their diverse reaction generate an enormous scope in the field of medicinal chemistry. The utility of pyrimidines as synthon for various biologically active compounds has given impetus to these studies. The pyrimidine derivatives show various biological activities including antitubercular, antioxidant, anti-inflammatory, anticonvulsant, antimicrobial, antibacterial, antiplasmodial, antifungal, anticancer, and analgesic activity. This article aims to review the recent works on pyrimidine derivatives together with the biological potential during the past years. © 2015, Asian Journal of Pharmaceutical and Clinical Research. All right reserved.


Kumar T.,Shri Rawatpura Sarkar Institute of Pharmacy | Gupta A.,Shri Rawatpura Sarkar Institute of Pharmacy | Gidwani B.,Shri Rawatpura Sarkar Institute of Pharmacy | Kaur C.D.,Shri Rawatpura Sarkar Institute of Pharmacy
International Journal of Biological Chemistry | Year: 2015

Euphorbia tithymaloidus is a shrub having a range of activities like anti-inflammatory, antioxidant, anti-malarial, anti-tuberculosis, antifungal, antibacterial, antidaibetic and wound healing etc., but the anthelminthic activity of this plant is still under consideration. In this study carried out the phytochemical and anthelminthic activity of Euphorbia tithymaloidus in order to estimate its potential in treating the intestinal worms. The in vitro anthelmintic activity was performed on ethanolic leaf extract using adult earthworms (Pheretima posthuma). The standard drug used was albendazole in the concentration of 15-60 mg mL-1 to note the paralysis time and death time. The result of the study showed that the ethanolic leaf extract shows the presence of certain phytoconstituents like flavonoids, carbohydrates, steroids and alkaloids. The reaction time was noted as paralysis time and death time for all the concentrations and compared with standard albendazole. The extract exhibited remarkable activity at a concentration of 45 mg mL-1 as compared to standard drug albendazole within a shorter time span of less than 10 min. © 2015 Academic Journals Inc.


PubMed | Shri Rawatpura Sarkar Institute of Pharmacy
Type: Journal Article | Journal: Advanced pharmaceutical bulletin | Year: 2013

The aim of present in vitro studies was performed to examine the antioxidant and anticancer activities of ethanol and aqueous extracts of Drosera indica L.Different concentrations (5 - 640mcg/ml) of the ethanol (EEDI) and aqueous (AEDI) extracts of D.indica L were used in various antioxidant assay methods such as hydroxyl radicals, DPPH, super oxide radical scavenging activity, chelating ability of ferrous ion, nitric oxide radical inhibition, ABTS and reducing power. Ascorbic acid (AA) was used as the standard antioxidant for the free radical scavenging assays. Daltons Ascitic Lymphoma (DAL) and Ehrlich Ascitic Carcinoma (EAC) cell lines were used as the in vitro cancer models for the tryphan blue dye and LDH leakage assays, where 5 to 250mcg /ml of both EEDI and AEDI were tested.EEDI showed antioxidant activities with the minimum IC50 values of 34.80.43 mcg/ml in scavenging of hydroxyl radical and moreover AEDI showed minimum IC50 values of 94.510.84 mcg/ml in Fe(2+)chelating assay. EEDI on the reducing power assay and ABTS showed higher IC50 than standard AA. IC50 values of AEDI on Fe(2+) chelating assay and super oxide radical assay was lesser than IC50 value of AA. Both extracts at 250mcg/ml dose showed remarkable increase in the percentage of dead cancer cells (90% by EEDI and 86% by AEDI in DAL model and 89% by EEDI and 80% by AEDI in EAC model).It is concluded from this study that D.indica L exhibited excellent antioxidant activity against the different in vitro antioxidant models and anticancer activity against the two different cell lines tested.

Loading Shri Rawatpura Sarkar Institute of Pharmacy collaborators
Loading Shri Rawatpura Sarkar Institute of Pharmacy collaborators