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Salmani Manjunath P.,Shri BM Patil Medical College | Mindolli P.B.,Al Ameen Medical College
Journal of Communicable Diseases | Year: 2011

A rapid test for diagnosis of malaria based on acridine orange staining of centrifuged blood samples in a microhaematocrit tube (QBC) was compared with Leishman stained thin peripheral blood smear in 287 samples. Malaria was diagnosed in 44 patients by Leishman staining technique and in 65 patients by QBC method. The QBC method allowed detection of an additional 21 cases. Thus the prevalence rate of malaria during the study was 22.65%. In 222 Patients who were negative by the QBC technique, the Leishman stained smears were also negative for malarial parasite. Although QBC method was superior to the smear for malarial parasite detection, species identification was difficult by this technique. The QBC method provides a reliable, quick, easily mastered, accurate method for diagnosis of malaria. The QBC system can also be used in the diagnosis of other parasitic diseases from blood (Filariasis). However, Leishman stained thin blood film still appear superior for species identification. Source


Tangeda P.R.,Prathima Institute of Medical science | Patil S.,Shri BM Patil Medical College | Shastri N.,Prathima Institute of Medical science | Noorali S.N.,Prathima Institute of Medical science
Journal of Clinical and Diagnostic Research | Year: 2016

Introduction: Anaemia affects various organs in body including the heart. In anaemia, oxygen carrying capacity of blood decreases. Iron depletion and the amount of stored iron are reduced in iron deficiency anaemia which limits red cell production. However, the studies which show the effect of anaemia on myocardial function during pregnancy are few in India. Aim: To study the effect of iron deficiency anaemia on myocardial function by ECG during second trimester of pregnancy and to compare ECG changes with normal pregnant women in second trimester. Materials and Methods: The study was conducted at antenatal OPD between Oct 2014 to Jul 2015. Hundred pregnant women were selected and divided into 2 groups. A total of 50 normal pregnant women (control group) in 2nd trimester (10-14 weeks of gestation) were compared with equal number of pregnant women with anaemia (study group) in 2nd trimester, aged between 20-30 years. Electrocardiogram was recorded using Philips twelve channel ECG machine model TC20 in both control and study groups to evaluate myocardial performance. Haematological parameters were analysed by SYSMEX auto analyser. Analysis of Variance (One way ANOVA) was used for comparison between study and control groups and the data was analysed by t-tests. Results: In our study a significant decrease in QRS duration and increase in QTc were observed in study group (p<0.05). T-wave abnormalities like flat and negative T-waves in lead II, III, avF, V2-V4 were more frequent (p<0.05). 90% of subjects in study group had tachycardia and ECG abnormalities. There was a negative correlation between Hb level, serum ferritin and tachycardia, ECG abnormalities. Conclusion: Pregnancy with Iron deficiency anaemia brings about various changes in ECG, suggesting that anaemia and volume overload in pregnancy is a risk factor that may lead to cardiac hypertrophy. © 2016 Journal of Clinical and Diagnostic Research. All Rights Reserved. Source


Salmani M.P.,Shri BM Patil Medical College | Mindolli P.B.,Shri BM Patil Medical College
Journal of Clinical and Diagnostic Research | Year: 2011

Rapid diagnosis of malaria is pre-requisite for effective treatment and reducing mortality and morbidity of malaria. In this study, Quantitative Buffy Coat (QBC) was compared with thick and thin peripheral blood smears and malaria antigen test. A total of 387 samples were collected from patients presenting with fever and chills. Malaria was diagnosed in 60, 72 and 56 patients by Leishman staining technique, QBC method and malaria antigen test respectively. The QBC method allowed an additional 12 cases. Thus the prevalence rate of malaria during the study was 18.6%. In 315 patients who were negative by the QBC, malaria antigen test and the Leishman stained smears were also negative for malarial parasite. Although QBC method was superior to the smear for malarial parasite detection, species identification was not possible in 32 cases by this technique. The QBC method has its advantages in terms of speed, sensitivity and ease, especially in an endemic area as ours. The QBC method helps in the diagnosis of jaundice, aplastic anaemia and kala-azar. The QBC system can also be used in the diagnosis of other parasitic diseases such as filariasis. However, Leishman stained thin blood smear still appear superior for species identification. Source


Pareek A.,Clinical Research Laboratories | Chandurkar N.,Clinical Research Laboratories | Thomas N.,Christian Medical College | Viswanathan V.,Hospital for Diabetes and Diabetes Research Center | And 13 more authors.
Current Medical Research and Opinion | Year: 2014

Objective:To compare efficacy and safety of hydroxychloroquine with pioglitazone in type 2 diabetes mellitus (T2DM). Research design and methods:This double-blind study randomized 267 uncontrolled type 2 diabetes patients (HbA1c ≥7.5% and ≤11.5%), post 3 months' treatment with glimepiride/gliclazide and metformin, to additionally receive hydroxychloroquine 400mg/day (n=135) or pioglitazone 15mg/day (n=132) for 24 weeks. Efficacy was assessed by changes in HbA1c, fasting (FBG) and post-prandial (PPG) blood glucose at Week 12 and Week 24. Results:At Week 12 and Week 24, HbA1c, FBG and PPG significantly reduced from baseline in both groups. Mean reduction in glycemic parameters at Week 12 (HbA1c: -0.56% vs -0.72%, p=0.394; FBG: -0.99mmol/L vs -1.05mmol/L, p=0.878; PPG: -1.93mmol/L vs -1.52mmol/L, p=0.423) and Week 24 (HbA1c: -0.87% vs -0.90%, p=0.909; FBG: -0.79mmol/L vs -1.02mmol/L, p=0.648; PPG: -1.77mmol/L vs -1.36mmol/L, p=0.415) was not significantly different between the hydroxychloroquine and pioglitazone groups. Change in total cholesterol (TC) and LDL-C was significant in favor of hydroxychloroquine (TC: -0.37mmol/L vs 0.03mmol/L, p=0.002; LDL-C: -0.23mmol/L vs 0.09mmol/L, p=0.003). Triglycerides significantly reduced in both groups at Week 24. Mean HDL-C remained unchanged. Study treatments were well tolerated. Conclusion:With favorable effects on glycemic parameters and lipids, hydroxychloroquine may emerge as well tolerated therapeutic option for T2DM. Limitations:The sample size for this study was small. However, based on the encouraging results of this proof-of-concept study, longer duration studies in larger population can be conducted to further confirm these findings. Trial registration details:Clinical Trial Registry-India URL: http://ctri.nic.in, Registration Number: CTRI/2009/091/001036. © 2014 All rights reserved. Source


Salmani M.P.,Shri BM Patil Medical College | Mindolli P.B.,Shri BM Patil Medical College | Vishtvanath G.,JJM Medical College
Journal of Communicable Diseases | Year: 2011

Chlamydia trachomatis has currently emerged as the most common sexually transmitted pathogen. It is usually asymptomatic and is difficult to diagnose clinically. It is one of the causes for bad Obstetric History (BOH) and infertility. Women at highest risk often have the least access to health care facilities. Therefore there is a need for a rapid, simple, inexpensive and non-invasive test to detect C. trachomatis infection. Serological testing forms the mainstay of diagnosing the disease and to treat BOH and infertility. Hence the present study was conducted. Enzyme linked immunosorbent Assay (ELISA) was used for detection of IgG antibodies against C. trachomatis. Out of 260 cases, 130 had history of BOH, 80 had history of infertility and 50 healthy pregnant women (HPW) were used as controls. The seropositivity of C. trachomatis in the study was 25.4% (66). Out of 130 cases of BOH, seropositivity was 27.7% (36). Out of 80 cases of infertility, seropositivity was 35% (28) and out of 50 cases of HPW seropositivity was 4% (2). In BOH cases, women with history of two abortions showed seropositivity of 7.3% and women with history of three or more abortions showed seropositivity of 62.5%. Hence, seropositivity of C. trachomatis infection was found to be significant among women with BOH and infertility as compared to HPW. Source

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