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Mehta Y.,Shree Ramkrishna Institute of Computer Education and Applied science | Buch S.,InfoSolutions
Proceeding - IEEE International Conference on Computing, Communication and Automation, ICCCA 2016 | Year: 2016

The raw unstructured big data created on social networking platform is having great potential to generate various trends. In this work, we propose a novel approach for meaningful social network data mining with integration of semantic web and linked open data concepts. We propose a semantic proximity based data mining process in our framework for the analysis of employment trends using LinkedIn. The data extraction algorithm and semantic based results are presented which describes the real time dataset and its integration with linked data. We compare different linked data formats to evaluate our results and identify our format of data expression. The process of social data analytics by integrating background knowledge from global linked data is the core concept of our research work. © 2016 IEEE.

Trivedi R.,Shree Ramkrishna Institute of Computer Education and Applied science
International Journal of Pharma and Bio Sciences | Year: 2016

Our aims is to clone the Glucose oxidase (GOx) gene which has been isolated from Aspergillus niger, in turn isolated from the fertile garden soil. Detection of the expression of gox gene in prokaryotic host. Aspergillus niger has been isolated from soil samples taken from Navsari region. Enzyme assay has been done to check enzyme. DNA extraction & PCR amplification was done using Banglore GeNeiTM kits. 90% success has been achieved by obtaining the GOx protein from Escherichia coli BL21 in inclusion bodies. The protein has been purified onto Ni-NTA column. gox gene was successfully isolated and expressed in prokaryotic host, which would be purified by simple procedure. -We conclude that the by-product of gluconic acid fermentation has a great industrial importance. So, for greater production of GOx, the gene can be transferred from donor to the recipient. The gox gene of Aspergillus niger has been isolated and studied for the identification & its expression for improvement of enzyme production by establishing a process based on heterologous expression.

Ghelani A.,Shree Ramkrishna Institute of Computer Education and Applied science | Ghelani A.,Hemchandracharya North Gujarat University | Patel R.,Hemchandracharya North Gujarat University | Mangrola A.,Hemchandracharya North Gujarat University | Dudhagara P.,Veer Narmad South Gujarat University
Genomics Data | Year: 2015

A taxonomic description of bacteria was deduced from 5.78. Mb metagenomic sequence retrieved from Tulsi Shyam hot spring, India using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome contained 10,893 16S rDNA sequences that were analyzed by MG-RAST server to generate the comprehensive profile of bacteria. Metagenomic data are available at EBI under EBI Metagenomics database with accession no. ERP009559. Metagenome sequences represented the 98.2% bacteria origin, 1.5% of eukaryotic and 0.3% were unidentified. A total of 16 bacterial phyla demonstrating 97 families and 287 species were revealed in the hot spring metagenome. Most abundant phyla were Firmicutes (65.38%), Proteobacteria (21.21%) and unclassified bacteria (10.69%). Whereas, Peptostreptococcaceae (37.33%), Clostridiaceae (23.36%), and Enterobacteriaceae (16.37%) were highest reported families in metagenome. Ubiquitous species were Clostridium bifermentans (17.47%), Clostridium lituseburense (13.93%) and uncultured bacterium (10.15%). Our data provide new information on hot spring bacteria and shed light on their abundance, diversity, distribution and coexisting organisms. © 2015 The Authors.

Dudhagara P.,Veer Narmad South Gujarat University | Ghelani A.,Shree Ramkrishna Institute of Computer Education and Applied science | Patel R.,Hemchandracharya North Gujarat University | Chaudhari R.,Hemchandracharya North Gujarat University | Bhatt S.,Hemchandracharya North Gujarat University
Genomics Data | Year: 2015

Bacterial diversity and archaeal diversity in metagenome of the Lonar soda lake sediment were assessed by bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome comprised 5093 sequences with 2,531,282. bp and 53 ± 2% G. +. C content. Metagenome sequence data are available at NCBI under the Bioproject database with accession no. PRJNA218849. Metagenome sequence represented the presence of 83.1% bacterial and 10.5% archaeal origin. A total of 14 different bacteria demonstrating 57 species were recorded with dominating species like Coxiella burnetii (17%), Fibrobacter intestinalis (12%) and Candidatus Cloacamonas acidaminovorans (11%). Occurrence of two archaeal phyla representing 24 species, among them Methanosaeta harundinacea (35%), Methanoculleus chikugoensis (12%) and Methanolinea tarda (11%) were dominating species. Significant presence of 11% sequences as an unclassified indicated the possibilities for unknown novel prokaryotes from the metagenome. © 2015 The Authors.

PubMed | Navsari Agricultural University, Sardar Vallabhbhai National Institute of Technology, Surat and Shree Ramkrishna Institute of Computer Education and Applied science
Type: | Journal: Journal of fluorescence | Year: 2016

In this work, highly luminescent carbon dots (CDs) were synthesized by the hydrothermal method at 170C for 12h using pasteurized milk as a carbon source. The prepared CDs exhibited bright blue fluorescence under UV light illumination at 365nm. The CDs show fluorescence life time of ~4.89ns at excitation wavelength of 370nm. The effect of different solvents on the fluorescence property of CDs was also investigated. The lisinopril (Lis)-loaded CDs were fabricated by self-assembly of lisinopril on the surfaces of CDs, which were characterized by UV-visible and FT-IR spectroscopic techniques. The controlled release of lisinopril from the Lis-CDs was realized at pH values of 5.2, 6.2 and 7.4, respectively. The results of the cytotoxicity and confocal laser scanning microscopic images indicate that the Lis-CDs were successfully uptaken by HeLa cells without apparent cytotoxicity. The synthesized CDs show great potential as drug vehicles with good biocompatibility, sustained release of lisinopril from CDs, indicating that the CDs can act as a promising drug delivery system for therapeutic delivery and/or bioimaging applications.

Chauhan K.,Shree Ramkrishna Institute of Computer Education and Applied science | Gulati R.,Veer Narmad South Gujarat University
Smart Innovation, Systems and Technologies | Year: 2016

Glaucoma is one of the second most important cause of blindness after cataracts. Detection of glaucoma is essential to prevent visual damage. In India, Glaucoma is the third leading cause of blindness. This paper discusses an algorithm developed for detection and diagnosis of glaucoma. The algorithm calculates the Cup to Disc Ratio (CDR) which is obtained through image processing of the retinal images obtained from stratus OCT from Sudhalkar Eye Hospital, Vadodara. Image processing has been carried out on 120 retinal images obtained from Stratus OCT out which 79 are glaucomatous eyes and 41 are normal eyes. OTSU histogram and watershed algorithm have been used for image segmentation. Image analysis has been carried out through image segmentation, which is the process of dividing an image into regions or object. Morphological operations have been performed for the enhancement of the images to extract optic cup and optic disc region from the eye. The accuracy of the algorithm is 94 %. MATLAB software has been used for image processing on the retinal images. © Springer International Publishing Switzerland 2016.

Toral D.,Shree Ramkrishna Institute of Computer Education and applied science
International Journal of ChemTech Research | Year: 2014

2-Hydroxy-5-Ethylacetophenone Oxime (HEAO) has been used as a reagent for the study of Ni (II) chelate. The reagent gives green precipitate at pH 7.0 to 8.0. Ni (II) is quantitatively precipitated by HEAO between pH 5.5 to 10.0 and it was determined gravimetrically at pH 7.0 to 8.0. After drying the precipitate at 110° C its composition corresponds to the formula Ni(C10H12O2 N)2. Structure of green coloured complex has been assigned on the basis of Job's method, mole-ratio method and IR spectra.

Ishani D.,Shree Ramkrishna Institute of Computer Education and Applied science | Tina S.,Shree Ramkrishna Institute of Computer Education and Applied science
Journal of Interdisciplinary and Multidisciplinary Research | Year: 2015

In recent years it has become widely recognized that bacteriophages have several potential applications in the food industry. They have been proposed as alternatives to antibiotics in animal health, as bio preservatives in food and as tools for detecting pathogenic bacteria throughout the food chain. Bacteriophages are viruses that only infect and lyse bacterial cells. Consequently, they display two unique features relevant in and suitable for food safety. Namely, Their safe use as they are harmless to mammalian cells and their high host specificity that allows proper starter performance in fermented products and keeps the natural microbiota undisturbed. However, the recent approval of bacteriophages as food additives has opened the discussion about 'edible viruses'. In this review, we examine the promising uses of phages for the control of foodborne pathogens and the drawbacks on which more research is needed to further exploit these biological entities.

Snehkunj R.,Shree Ramkrishna Institute of Computer Education and Applied science | Mehta R.,Sk Patel Institute Of Management And Computer Studies | Jani A.N.,Sk Patel Institute Of Management And Computer Studies
Advances in Intelligent Systems and Computing | Year: 2016

In the current scenario of Biomedical Research, the Magnetic Resonance Imaging (MRI) images visual analytics processing applications are facing challenges of the use of different techniques under different frameworks supported by different software tools. The establishment of different frameworks under different software tools and migration of data from one framework to another as well as one tool or environment to another poses critical difficulties and large time consumption. To reduce this hassle, the need of common framework is identified and the same is undertaken in this work. To address this issue, a framework MRI Image Pattern Detection Framework (MIPDF) is proposed to take care for the reduction of cited hassle and improved visual analytics to support medical professionals in their act of detection and diagnosis of diseases by identifying regularity and irregularity with improved visualization and analytics results. © Springer India 2016.

Shah K.B.,Institute of Management Sciences | Trivedi R.,Shree Ramkrishna Institute of Computer Education and Applied science
International Journal of Pharma and Bio Sciences | Year: 2012

An extracellular phytase from Aspergillus tamari was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromatography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maximal phytate-degrading activity at pH 8.5. Optimum temperature for the degradation of phytate was 28°C. The kinetic parameters for the hydrolysis of nitro phenyl phosphate disodium salt were determined to be Km = 54 μmol-land kcat = 190 sec-1 at pH 8.5 and 28°C. The purified enzyme was rather specific for phytate dephosphorylation. It was shown that the phytase preferably dephosphorylates myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-Ins(1,2,4,5,6)P5, DIns(1,2,5,6)P4, D-Ins(1,2,6)P3, D-Ins(1,2)P2 to finally Ins(2)P.

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