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Ambasana M.A.,Shree M And N Virani Science College | Kapuriya N.P.,Shree M And N Virani Science College | Faldu N.J.,Shree M And N Virani Science College | Ladva K.D.,Shree M And N Virani Science College
Der Pharmacia Lettre | Year: 2014

The current study was carried out to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of Ticagrelor in bulk form. The solvent used throughout the experiment was Methanol. Absorption maxima (λmax) of the Ticagrelor were found to be 224 and 255 nm. The Beer's law was obeyed in the range of 2-7μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.141x + 0.125 with correlation coefficient R2 of 0.998. The recovery values for Ticagrelor ranged from 99.59% to 101.66%. The % RSD was 0.37% and 0.39%, respectively for intraday and interday precision. The limit of detection and limit of quantification was 0.05μg/mL and 0.20μg/mL respectively. Method was statistically validated for accuracy, precision, linearity and solution stability; hence it is directly applicable for the determination of Ticagrelor in API and bulk form.

Senthilkumar K.P.,Shree M And N Virani Science College | Senthilkumar K.P.,Madura College Autonomous | Thirumurugan R.,Bharathidasan University
Molecular Biology Reports | Year: 2014

Tobacco use and environmental air pollution are the established etiological factors in head and neck cancer (HNC) progression. Nevertheless, not all the inhabitants with high usage of tobacco from the same polluted locality are suffering with HNC and this is due to the existence of factors like inter-individual genetic polymorphisms, life time exposure to tobacco and the rate of xenobiotic metabolism enzyme (XME) activity. The present study investigates the polymorphic genotypes of the most important XME, glutathione-S-transferase Mu 1 (GST M1) and Theta 1 (GST T1) as the risk modulator to HNC among tobacco-habituated inhabitants of Saurashtra in Gujarat, a region in western India. A population based case-control study was done in 252 HNC patients and 504 healthy controls. Blood samples were collected from the subjects and investigated for polymorphic genotypes of GST M1 and GST T1. Estimation of the odds of risks was done by logistic regressions. Among the subjects with high usage of tobacco, M1 not null-T1 null genotypes presence was found as risk reducing factor to HNC with 0.334 folds (95 % CI; 0.170-0.659). The presence of M1 null-T1 not null genotypes was found with susceptibility to HNC among the subjects with no habit of tobacco chewing, adjusted odds ratio (AOR) 3.170 (1.128-8.913) and no habit of smoking, AOR of 2.544 (1.094-5.963). The present study reveals the finding of significantly increased risk to HNC by interactions of GST M1 null-GST T1 not null polymorphic genotypes among the subjects with nil or less tobacco usage shed some light for the insights of biomarker application in early detection of HNC. © 2014 Springer Science+Business Media.

Senthilkumar K.P.,The Madura College Autonomous | Senthilkumar K.P.,Shree M And N Virani Science College | Thirumurugan R.,The Madura College Autonomous | Thirumurugan R.,Bharathidasan University
Asian Pacific Journal of Cancer Prevention | Year: 2012

Background: Tobacco contains agents which generate various potent DNA adducts that can cause gene mutations. Production of DNA adducts may be neutralized by glutathione S transferase (GST) along with other phase I and phase II enzyme systems. The existence of null type of GST among the population increases the susceptibility to various disorders and diseases. The present study focuses on the impact of high tobacco usage and possible null type mutation in GST loci. Methods: Genotypes of GST were detected by multiplex polymerase chain reaction in unrelated 504 volunteers of high tobacco using natives of Gujarat. Allelic frequencies were calculated using Statistical Package for Social Studies-16 software. Hardy Weinberg Equilibrium (HWE) was calculated using Chi square test. Two sided Fisher's significance test was used to compare allelic frequencies of different populations. Results: The frequency of homozygous null genotype of GSTM1 and GSTT1 were 20% (95% CI 16.7-23.9) and 35.5% (95% CI 31.4-39.9) respectively. The GSTM1 and GSTT1 null allele frequency distribution in the Gujarat population was significantly deviating from HWE. GSTT1 null frequency of Gujaratians was significantly higher and different to all reported low tobacco using Indian ethnics, while GSTM1 was not differing significantly. Conclusion: Tobacco usage significantly influences the rate of mutation and frequency of GSTT1 and M1 null types among the habituates. The rate of mutation in GSTT1 loci was an undeviating response to the dose of tobacco usage among the population. This mutational impact of tobacco on GSTT1 postulates the possible gene - environment interaction and selection of null genotype among the subjects to prone them under susceptible status for various cancers and even worst to cure the population with GSTT1 dependent drugs.

Senthilkumar K.P.,The Madura College Autonomous | Senthilkumar K.P.,Shree M And N Virani Science College | Thirumurugan R.,The Madura College Autonomous | Thirumurugan R.,Bharathidasan University
Asian Pacific Journal of Cancer Prevention | Year: 2012

Background: Glutathione-S-transferase (GST) is an important phase II xenobiotic compound metabolizing enzyme family, involved in tolerance to a particular drug or susceptibility to a diseasec. This study focused the GSTM1 and T1 null allele frequency in the Gujarat population with a comparison across other Inter- and Intra-Indian ethnic groups to predict variation in the possible susceptible status. Methods: DNA was isolated by a salting out method and GSTM1 and T1 homozygous null genotypes were detected by multiplex polymerase chain reaction in 504 unrelated individuals. The genotype distribution of null alleles was compared with Indian and non Indian ethnics reported earlier in the literature using Fisher's test. Results: The frequencies of the homozygous null genotypes of GSTM1 and GSTT1 were 20% (95%CI 16.7-23.9) and 35.5% (95%CI 31.4-39.9) respectively. GSTM1 null frequency did not deviate from most other Indian ethnic groups but differed from the majority of those of non Indian ethnicity studied. The frequency of homozygous null type of GSTT1 was significantly higher and deviated from all Indian groups and a few of non Indian ethnicity. Conclusions: Gujarat ethnicity, possibly the most susceptible for GSTT1 dependent drug disposition and diseases regarding effects of pollution. Further, the results have implications for GSTT1 dependent drugs used for treatment, a serious problem which needs to be solved by physicians and clinical researchers.

Padhariya N.,Indraprastha Institute of Information Technology | Raichura K.,Shree M And N Virani Science College
2014 7th International Conference on Contemporary Computing, IC3 2014 | Year: 2014

Crowdlearning is an incentive-based learning platform for crowd, denoted as iCL, to inspire experts for knowledge sharing and to incentivize them for their contributions in crowd. In iCL, collaborators provide learning platform to the crowd of interested learners. The relativeness of interest of contributors and learners is compared by match-making process. The core objective of iCL is to motivate learners to join common-interest crowd and to collaboratively perform Crowdlearning activities. Moreover, incentivization motivates learners to grow crowd. The main contributions of iCL are three-fold. First, it presents the architecture of iCL with the crowd formation process that includes the processes of initialization and collaboration. Second, it proposes three incentive-based fees distribution schemes defined as Equally Distributed Incentive (EDI), Level-based EDI (LEDI) and Commission-oriented LEDI (CLEDI). Third, our performance evaluation shows that our schemes are indeed effective to motivate learners for participation and to inspire experts for their contribution through incentivization in crowdlearning. © 2014 IEEE.

Thumar J.T.,Shree M And N Virani Science College | Dhulia K.,Shree M And N Virani Science College | Singh S.P.,Saurashtra University
World Journal of Microbiology and Biotechnology | Year: 2010

A halotolerant alkaliphilic actinomycete, Kut-8, was isolated from saline desert of Kutch, Western India. It has been identified as Streptomyces aburaviensis based on the chemotaxonomic characteristics, including cell wall constituents. Kut-8 is Gram-positive having a spiral sporophore with dark green and fluffy spore mass. It was able to grow with 15%, w/v NaCl with optimum being in the range of 5-10%. It grew optimally at pH 9 with slow growth at neutral pH. The cell wall contained l-diaminopimelic acid and no diagnostic sugars. It produced an antibiotic that selectively inhibited the growth of Gram-positive bacteria, with Bacillus subtilis being the most sensitive. Kut-8 secreted the antibiotic optimally during mid-stationary phase (on day 14 of growth in liquid culture). The crude antibiotic metabolites were separated by various solvent systems with hexane-methanol-water giving the best separation. The results of bioautographs revealed the presence of single active compound in the Kut-8 antibiotic filtrate. Partial purification of antibiotic metabolite by charcoal absorption and methanol extraction resulted in enhanced antimicrobial activity by 4.16-fold. The study holds significance as only few salt-tolerant alkaliphilic actinomycetes from saline deserts have been explored and information on their antimicrobial potential is still scarce. © 2010 Springer Science+Business Media B.V.

Prakash N.,Shree M And N Virani Science College | Gareja D.A.,Shree M And N Virani Science College
Journal of Proteomics and Bioinformatics | Year: 2010

Cheminformatics is the application of computational methods to chemical problems, with particular emphasis on the manipulation of structural information. Cheminformatics is a relatively new field of information technology that focuses on the collection, storage, analysis, and manipulation of chemical data. The chemical data of interest typically includes information on small molecule formulas, structures, properties, spectra, and activities (biological or industrial). Cheminformatics originally emerged as a vehicle to help the drug discovery and development process; however Cheminformatics now plays an increasingly important role in many areas of biology, chemistry, and biochemistry. Cheminformatics can also be applied to data analysis for various industries like paper and pulp, dyes and such applied industries. © 2010 Prakash N, et al.

De B.K.,Shree M and N Virani Science College | Dahyabhai Patel J.,Shree M and N Virani Science College
Journal of Oleo Science | Year: 2010

In a unique attempt modification of only palm oil has been investigated. Triacylglycerols of palm oil has been randomized by chemical and biochemical means. Chemical randomization was carried out us-ing sodium methoxide (NaOMe: 0.4 to 0.6%, w/w) whereas the biochemical modifications were performed using five different commercial lipases from Amano Enzymes. It was observed that after chemical random-ization (for 15 minutes at 90 °C) using sodium methoxide (0.5%, w/w) catalyst the melting point of refined palm oil has risen from 32.0°C to 40.1°C. Chemical treatments for 15 minutes at 60°C in miscella phase (60%, w/v oil in hexane) using 0.5%, w/v sodium methoxide resulted increase in melting point from 32.0°C to 42.0°C. After enzymatic treatment using lipases it was observed that the melting point may rise from 32.0°C to 38.5°C (in 15 minutes at 45.0°C). All the five enzymes were found to be active in respect of randomization capacity and active at very low concentration 0.004 to 0.010% (w/w). © 2010 by Japan Oil Chemists' Society.

Vishwakarma N.P.,Shree M And N Virani Science College | Jadeja V.J.,Shree M And N Virani Science College
Plant Signaling and Behavior | Year: 2013

miRNAs are endogenous approx 22 nucleotide RNA which mediates transcriptional or Post-transcriptional gene regulation and play a critical role in diverse aspects of plant development. miRNA identification in wet lab have various constraints, it is time consuming and expensive. It also faces the limitation of identifying miRNAs expressed at specific time and/or at special conditions. Due to the nature of strong conservation of miRNA in plant species, the use of comparative genomics approach for expressed sequence tags (ESTs), Genome Survey Sequence (GSS) and structural feature criteria filter has paved the way toward the identification of conserved miRNAs from the plant species whose genomes are not yet available in public domain. To identify the novel miRNA from Jatropha curcas, a total of 46862 EST sequences and 1569 GSS were searched for homology to previously known viridiplantae 2502 mature miRNA. After predicting the RNA secondary structure, 24 new potential miRNA were identified in J. curcas. Using the newly identified miRNA sequences, a total of 78 potential target genes were identified for 3 miRNA families. Most of the miRNA targeted genes were predicted to encode transcription factors that regulate cell growth and development, signaling, and metabolism. These findings considerably broaden the scope of understanding the functions of miRNA in J. curcas. © 2013 Landes Bioscience.

Raichura K.,Shree M And N Virani Science College | Padhariya N.,Indraprastha Institute of Information Technology
Proceedings - IEEE International Conference on Mobile Data Management | Year: 2014

This work proposes the edPAS system for the efficient processing and handling of dynamic parking allocation requests in vehicular networks. In edPAS, base-station and communicators collaboratively provide the effective event-based parking allocation process for vehicles. Edpas offers the best available parking-lot to vehicle, which is relevant to its event-place. Furthermore, edPAS tracks the vehicles through communicators for providing runtime changes in allotted parking-lot, if any, to the vehicles, thereby optimizing the parking-lot utilization. The main contributions of edPAS are three-fold. First, it proposes the architecture of edPAS with static and dynamic-allocation processes. Second, it proposes two parking-lot allocation schemes, FCFS and PR, using first-come-first-serve and priority algorithms respectively, for parking-queue mechanism. Third, our performance evaluation shows that our schemes are indeed effective for improving the edPAS functionality in terms of maximizing parking-lot utilization and reducing overall time at low communication cost. © 2014 IEEE.

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