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Guo X.-F.,Zaozhuang Municipal Hospital | Wang A.-Y.,Shouguang Peoples Hospital | Liu J.,Taixing Peoples Hospital
European Review for Medical and Pharmacological Sciences | Year: 2016

OBJECTIVE: In this study, we investigated whether miR-33a downregulation in HCC is a result of hypoxia-inducible factors (HIFs) overexpression. Then, we further studied the regulative effects of miR-33a on Twist1 and their regulation in HCC cell invasiveness. MATERIALS AND METHODS: Human hepatocellular cancer (HCC) cell lines (HepG2 and BEL-7402) were transfected with miR-33a mimics, HIFs siRNA or Twist1 siRNA. MiR-33a level was measured using QRT-PCR. The binding between miR-33a and Twist1 3'UTR was verified using Western blot analysis and dual luciferase assay. E-cadherin and N-cadherin expression levels were detected by western blot analysis. Tumor cell invasion was assessed using transwell assay. RESULTS: MiR-33a downregulation in HCC cells is hypoxia-induced and is a result of HIFs upregulation. HIF-1α and HIF-2α suppression partly rescued miR-33a expression under hypoxia. Both HepG2 and BEL-7402 cells with miR-33a overexpression had significantly decreased E-cadherin expression and increased N-cadherin level. Transwell analysis confirmed that miR-33a overexpression significantly suppressed the tumor cell invasion capability. Twist1 is a direct target of miR-33a in HCC. HepG2 cells with Twist1 knockdown had significantly increased E-cadherin, decreased N-cadherin and suppressed invasion capability. CONCLUSIONS: MiR-33a downregulation in HCC cells is hypoxia-induced and is a result of HIFs upregulation. MiR-33a can modulate EMT and invasion of hepatocellular cancer cells at least partly via downregulating Twist1.


Li X.,Shouguang Peoples Hospital | Ma F.,Peoples Hospital Of Binzhou | Jia K.,Laiyang Central Hospital
Medical Science Monitor | Year: 2014

Background: Although (EEN) is a relatively safer route by which to feed patients with severe acute pancreatitis (SAP) or predicted SAP (pSAP) compared to total parental nutrition (TPN), the appropriate starting time for EEN administration after admission is still controversial. This study pooled all relevant studies to assess the complications associated with EEN by stratifying relevant RCTs into subgroups according to the starting time (<24 h or between 24 and 72 h after admission).Material/Methods: Relevant studies were searched for among 5 databases. The association between intervention and complications, including pancreatic infection, mortality, hyperglycemia, organ failure, and catheter-related septic complications, were assessed by using pooled risk ratio (RR) and the corresponding 95% confdential interval (CI).Results: Twelve RCTs were identifed through our literature search. Pooled analysis showed that EEN, but not TPN or delayed enteral nutrition (DEN), is associated with reduced risk of pancreatic infection, mortality, organ failure, hyperglycemia, and catheter-related septic complications. EEN within 24 h of admission presented signifcant-ly better outcome in morality than EEN between 24 and 72 h. However, no signifcant heterogeneity was observed in the risk of pancreatic infection, organ failure, hyperglycemia, and catheter-related septic complications between the 2 subgroups.Conclusions: If the patients are reasonably expected to have high compliance to EN therapy, it could be considered as early as possible. © Med Sci Monit, 2014.


Chang B.Y.,Shouguang Peoples Hospital
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2011

To explore the therapeutic effect of debridement and vacuum sealing drainage (VSD) of cavitas medullaris for the treatment of chronic osteomyelitis of tibia. From March 2006 to May 2009, 19 patients with chronic osteomyelitis of tibia were treated by debridment and VSD, then the second operation were performed to close the wound. Among them, 12 patients were male and 7 patients were female, the average age was 39 years (ranged from 25 to 68 years). The course of disease were from 10 months to 5 years. The main clinical symptoms were red swelling, tenderness and fluid of local soft tissue. There were prolonged unhealed sinus and pus; the X-ray showed osteosclerosis, increased bone mineral, and sequestrum and dead space was formed. The result of bacterial culture showed 3 cases were aeruginosus bacillus, 13 cases staphylococcus aureus, 1 case bacillus aerogenes and 2 cases beta streptococcus. Among them, 3 cases were methicillin resistant staphylococcus (MRS). After debridement and VSD of cavitas medullaris 18-22 days later, the granulation tissue grow well and the wounds of the 19 patients all healed primarily with direct suturing of 17 cases, loco-regional flap of 2 cases. The standard of wound healing was the dryness, cleanness and no drainage. The X-ray revealed the bone tissue grew well and no relapse and fracture occurred during followed-up 6-12 months. The debridement and VSD of cavitas medullaris is a very effective and safe treatment for chronic osteomyelitis of tibia.


Zhao C.,Weifang Medical University | Sun J.,Shouguang Peoples Hospital | Fang C.,Weifang Medical University | Tang F.,Zhejiang University
Inflammation | Year: 2014

Eucalyptol, also known as 1,8-cineol, is a monoterpene and has been shown to exert anti-inflammatory and antioxidant effect. It is traditionally used to treat respiratory disorders due to its secretolytic properties. In the present study, we evaluated the effect of 1,8-cineol on pulmonary inflammation in a mouse model of acute lung injury. We found that 1,8-cineol significantly decreased the level of TNF-α and IL-1β, and increased the level of IL-10 in lung tissues after acute lung injury induced by lipopolysaccharide (LPS). It also reduced the expression of nuclear factor kappa B (NF-κB) p65 and toll-like receptor 4 (TLR4), and myeloperoxidase activity in lung tissues. In addition, 1,8-cineol reduced the amounts of inflammatory cells in bronchoalveolar lavage fluid (BALF), including neutrophils and macrophages, and significantly decreased the protein content in BALF and the lung wet/dry weight (W/D) ratio. Its effect on LPS-induced pulmonary inflammation was associated with suppression of TLR4 and NF-κB expressions. Our results provide evidence that 1,8-cineol inhibits acute pulmonary inflammation, indicating its potential for the treatment of acute lung injury. © 2013 Springer Science+Business Media New York.


Zhou Y.,Shouguang Peoples Hospital | Liu J.,Shouguang Peoples Hospital | Zhang M.,Shouguang Peoples Hospital
International Journal of Clinical and Experimental Pathology | Year: 2016

Objective: The aim of this study was to explore the association between Interleukin-10 (IL-10) gene rs1518111 and rs3021094 single nucleotide polymorphisms (SNPs) and diabetic nephropathy (DN) susceptibility. Methods: A cohort of 265 Chinese Han population were enrolled in this case-control study. IL-10 gene rs1518111 and rs3021094 SNP were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) approach. Chi-square test was employed to analyze the differences of genotype and allele frequencies of the two polymorphisms between case and control groups. Odds ratios (ORs) and 95% confidence intervals (CIs) were applied to explain relative susceptibility of DN. Results: AA genotype and A allele frequencies of IL-10 SNP rs1518111 were significantly increased in cases compared with controls (P < 0.05), suggesting significant association with susceptibility of DN (OR = 3.091, 95% CI = 1.037-9.209; OR = 1.615, 95% CI = 1.083-2.406). Nevertheless, rs3021094 is weakly associated with DN risk (P > 0.05). Strong linkage disequilibrium (LD) level (D' = 1.0) was observed for IL-10 SNPs rs1518111 and rs3021094, and haplotype (G-A) frequencies in cases were significantly differ from controls (P < 0.05), revealing haplotype G-A could decrease the risk of DN occurrence (OR = 0.602, 95% CI = 0.397-0.913). Conclusion: IL-10 gene rs1518111 SNP might be associated with ND risk while rs1518111 were not in a Chinese Han population. A allele of rs1518111 was suggested to be a risk factor for DN. Haplotype G-A for rs1518111 and rs3021094 could be protective against DN susceptibility.


Yang Y.-C.,Weifang Yidu Central Hospital | Li Y.,Weifang Yidu Central Hospital | Guo M.-T.,Shouguang Peoples Hospital | Zhao J.-M.,Jining No 1 Peoples Hospital
International Journal of Clinical and Experimental Pathology | Year: 2016

Present study aimed to investigate the effect of cantharidin on reduction in cell viability and induction of apoptosis in KOSC-2 oral cancer cells. Cantharidin treatment for 48 h caused a concentration dependent reduction in KOSC-2 cell viability. The IC50 of cantharidin against KOSC-2 cells was found to be 50 nM after 48 h. KOSC-2 cells showed reduction in size, protrusion of membrane and condensation of nuclear material on treatment with 50 nM concentration of cantharidin for 48 h. Flow cytometry using propydium iodide staining revealed significant (P<0.05) increase in the population of cells in G0/G1 phase of cell cycle in cantharidin treated compared to the control cells. Results from western blot analysis showed increase in the expression of pro-apoptotic BAD and decrease in the expression of anti-apoptotic protein Bcl-2 on treatment with cantharidin in KOSC-2 cells. Cantharidin treatment for 48 h also resulted in a significant increase in the expression of Apaf-1 and AIF as well as translocation of cytochrome c into the cytosol. The activation of caspase-3 in KOSC-2 cells was enhanced significant (P<0.05) in cantharidin treated compared to the control cells. Thus cantharidin treatment inhibits KOSC-2 cell viability and induces apoptosis through mitochondrial pathway. Therefore, cantharidin can be used for the treatment of oral cancer.


PubMed | Nantong University and Shouguang Peoples Hospital
Type: Journal Article | Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico | Year: 2016

Anti-cancer effect of metformin on different kinds of lung cancer has been studied frequently. However, the association between metformin and the prognosis of lung cancer in type 2 diabetes patients is still controversial.An electronic search was conducted using PubMed/Medicine, EMBASE and Cochrane library databases. Statistical analyses were carried out using either random-effects or fixed-effects models according to the heterogeneity examined by I (2) statistics.Six studies involving 2350 patients were included in the current meta-analysis. In all, the pooled HR of overall survival (OS) was 0.90 (95% CI 0.84-0.96; P=0.003). Sub-group analysis showed that when stratified by region the HR of OS was 0.52 (95% CI 0.31-0.87; P=0.012) and 0.86 (95% CI 0.67-1.11, P=0.361) for Asian and Western countries. When stratified by study design, the HR of OS was 0.78 (95% CI 0.52-1.15, P=0.206) and 0.82 (95% CI 0.59-1.16, P=0.264) for cohort and medical data-based studies. When stratified by lung cancer subtype, HR of OS was 0.52 (95% CI 0.31-0.87; P=0.012), 1.06 (95% CI 0.51-2.19; P=0.878) and 0.82 (95% CI 0.59-1.16; P=0.264) for SCLS, NSCLC and non-divided subtypes, respectively.Metformin use may associate with a good prognosis in lung cancer patients with type 2 diabetes but the effect was modest. However, it could achieve benefits in a selective sub-group of lung cancer patients especially in SCLC patients from Asian. Further studies are warranted to confirm this efficacy.


PubMed | University of Manitoba, Xuzhou Medical College, Shouguang Peoples Hospital and Soochow University of China
Type: Journal Article | Journal: International immunopharmacology | Year: 2015

Interleukin-22 (IL-22) is a member of the IL-10 cytokine family that has recently gained attention in regard to its recognized pathogenic role in neurological and autoimmune disorders. The pathological involvement of IL-22 has been linked to Th17 cells that are involved in its production. Its biological activity results from its ability to bind to a heterodimeric receptor consisting of IL-22 receptor 1 (IL-22R1) and IL-10R2. Emerging evidence has identified IL-22 involvement in neurological diseases and autoimmune disorders such as Guillain-Barr Syndrome (GBS), multiple sclerosis (MS), Alzheimers disease (AD), encephalitis, inflammatory myopathies, myasthenia gravis (MG), systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), Sjogrens syndrome (SS), psoriasis and Crohns disease (CD). However, the biological activity of IL-22 is variable resulting in protective or pathogenic effects in different disease states. As such, the development of therapeutic targeting strategies to modify the biological activity of IL-22 is being explored as a promising interventional approach to treat neurological and autoimmune diseases.


PubMed | Liaocheng Peoples Hospital, Shouguang Peoples Hospital, Shandong University and The 148th Hospital of PLA
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016

BACKGROUND Postoperative axial symptoms (post-AS) after single-door cervical laminoplasty for cervical spondylotic myelopathy (CSM) are a common and severe complication that adversely affects normal daily activities. Their etiology remains unclear. It is important to know which preoperative factors are the most predictive of post-AS. Therefore, this study aimed to elucidate the preoperative factors affecting post-AS. MATERIAL AND METHODS A total of 102 patients with CSM who underwent single-door cervical laminoplasty between 2009 and 2015 were studied. According to operation date, patients were prospectively assigned to treatment with conventional laminoplasty (CL) or modified laminoplasty (ML). Preoperative clinical and radiological parameters were recorded. The incidence of post-AS with 2 procedures was compared prospectively. Multivariate analysis was used to determine the preoperative factors affecting post-AS. RESULTS The incidence of post-AS after ML was significantly lower than after CL (P=0.010). ML and preoperative cervical C2-7 Cobb angle (CCA) were signicant protective factors against post-AS (ML: P=0.011, odds ratio=0.302; CCA: P=0.042, odds ratio=0.947). Patients with post-AS had a lower preoperative CCA than patients without post-AS (P=0.043). The other preoperative factors were not significantly associated with post-AS. CONCLUSIONS The results of this study suggest that choosing ML procedure or selecting patients with high preoperative CCA can reduce the incidence of post-AS after single-door cervical laminoplasty for CSM, and that the other preoperative clinical or radiological parameters are less critical.


PubMed | Affiliated Hospital of Weifang Medical College, Shandong Provincial Hospital and Shouguang Peoples Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

MicroRNA-494 (miR-494) expression is aberrant in various types of human cancer. However, the prognostic value of miR-494 in pancreatic cancer remains unclear. The level of miR-494 expression was determined in 99 pairs of primary pancreatic cancer and their corresponding, adjacent non-tumor tissues by using quantitative reverse transcriptase polymerase chain reaction. We also analyzed the associations between miR-494 expression and clinicopathological features. The survival correlations were analyzed by using the Kaplan-Meier method and Cox proportional hazards model. The level of miR-494 expression was significantly downregulated in pancreatic cancer tissues (mean relative expression level SD, 0.48 0.11) as compared to matched adjacent normal tissues (1.80 0.28, P < 0.05). We found significant correlations between the miR-494 expression levels and TNM stage (P = 0.009), lymphatic invasion (P = 0.036), vascular invasion (P = 0.011), distant metastasis (P = 0.007), and tumor grade (P = 0.031). Pancreatic cancer patients with a low miR-494 expression level had a shorter overall survival than those with a high miR-494 expression level (P < 0.05). Reduced miR-494 expression in pancreatic cancer tissues is correlated with tumor progression and might be an independent, poor prognostic factor for patients with pancreatic cancer.

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