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Hiroshima-shi, Japan

Watanabe M.,Hiroshima Kokusai Gakuin University | Ichinose K.,Hiroshima Kokusai Gakuin University | Sasano K.,Shokkyo Co. | Ozaki Y.,Satake Co. | And 3 more authors.
Journal of Bioscience and Bioengineering | Year: 2011

In this research, the protein participation that occurs with the flocculation and sedimentation phenomena caused by adding enzymes to solid particles derived from rice washing drainage and the electrostatic properties of the solid particle surfaces was examined. As seen in the results, adding protease to rice washing drainage decreased the protein content, strengthened the negative surface electron charge, and decreased the isoelectric point (IEP) value of the solid particles. In addition, about 25% of the proteins in the rice washing drainage still remained after enzyme treatment. Moreover, due to the enzyme treatment, specific and gradual degradations of 10, 13, and 16. kDa of prolamin and 20-22. kDa of gluterin basic subunit, both categorized in the basic protein group, were observed in an SDS-PAGE analysis. These results suggest that the flocculation and sedimentation of solid particles in rice washing drainage occur as a result of the formation of the IEP-dependent strengthening of the negative surface electron charge on the solid particle surface. © 2011 The Society for Biotechnology, Japan. Source

Watanabe M.,Yamagata University | Makino M.,Satake Co. | Kaku N.,Yamagata University | Koyama M.,Shinshu University | And 2 more authors.
Journal of Bioscience and Bioengineering | Year: 2013

A newly isolated lactic acid producing bacterium Lactobacillus rhamnosus M-23, from a rice washing drainage storage tank was found to produce l-(+)-lactic acid from a non-sterilized mixture of rice washing drainage and rice bran without any additions of nutrients under the simultaneous saccharification and fermentation (SSF) process. The maximum lactate yield attained was 59 g/l with a productivity of 1.23 g/l/h and a product optical purity of 95% corresponding to a conversion of 0.85 g of lactic acid per gram of sugar equivalent. © 2012 The Society for Biotechnology, Japan. Source

Watanabe M.,Yamagata University | Maeda I.,Utsunomiya University | Koyama M.,Shinshu University | Nakamura K.,Shinshu University | Sasano K.,Shokkyo Co.
Journal of Bioscience and Bioengineering | Year: 2014

We studied a process that enables simultaneous recovery of protein and phosphorus compounds from rice bran. Phosphorus substances in full-fat and defatted rice bran such as phytic acid and inorganic ions were solubilized under acidic conditions in the first step. After that, inorganic and/or organic phosphate salts were recovered in insoluble form under weak alkaline conditions. Furthermore, protein fractions obtained after phosphorus compounds had been removed were solubilized under alkaline conditions. After solubilization, protein fractions with high content were recovered by isoelectric precipitation (IP) followed by electrolyzed water treatment (EWT). The highest protein content (52.3 w/w%) was attained when machine defatted rice bran was treated through the process. Energy-dispersive X-ray spectroscopy (EDX) and inductively coupled plasma atomic emission spectrometry (ICP-AES) analyses demonstrated efficient desalting from the protein fractions by EWT and higher phosphorus contents (15.1-16.4 w/w% P) in the phosphorus fractions compared with commercial phosphate rock. In addition, no heavy metal ions in either protein or phosphorus fractions were detected. These results suggest that the newly developed process is suitable for practical recovery of highly concentrated protein and phosphorus compounds from rice bran without enzymes or chemicals such as organic solvents, buffering agents, and surfactants. © 2014 The Society for Biotechnology, Japan. Source

Ijiri D.,Kagoshima University | Nakamura S.,Kagoshima University | Tatsugawa K.,Shokkyo Co. | Ijiri S.,Shokkyo Co. | Ohtsuka A.,Kagoshima University
Journal of Poultry Science | Year: 2013

This study was conducted to examine the effect of feeding concentrated rice-washing water (CRWW) on growth performance and lipid peroxidation levels in broiler chickens. CRWW (89.8% moisture) was dried at either a high (130°C) or low temperature (60°C). Three experimental diets were formulated as follows: 1) control diet; 2) diet containing 20% high-temperature dried CRWW (HT-CRWW); and 3) diet containing 20% low-temperature dried CRWW (LT-CRWW). Twenty-one chicks were divided into a control group and two treatment groups (seven chicks per group) at 15 days of age. The control group was fed the control diet, and the treatment groups were fed either an HT-CRWW or LT-CRWW-containing diet for 12 days. The growth performance (body weight, weight gain, feed intake, and feed efficiency) and the weight of the pectoral muscles were not different between treatment groups. Both HT-CRWW and LT-CRWW had a higher total polyphenol content and free radical scavenging activity than corn meal. Malondialdehyde (MDA) content, a marker of lipid peroxidation, was significantly lower in the skeletal muscle of chicks fed the HT-CRWW-containing diet compared with chicks fed the control diet; however, MDA content in chicks fed the LT-CRWW-containing diet did not differ from that of controls. CRWW has promise as a high-energy feed resource, and CRWW dried at high temperatures might affect lipid peroxidation in broiler chickens through higher antioxidant activity. © 2013, Japan Poultry Science Association. Source

Ogino A.,Japan National Agriculture and Food Research Organization | Ishida M.,Japan National Agriculture and Food Research Organization | Ohmori H.,Japan National Agriculture and Food Research Organization | Tanaka Y.,Japan National Agriculture and Food Research Organization | And 6 more authors.
Journal of Environmental Quality | Year: 2012

Life cycle assessment (LCA) was used to compare the greenhouse gas (GHG) emissions and energy consumption of three methods used to produce animal feed from concentrated rice-washing water (CRW) and disposing of the rice-washing water through wastewater treatment. Four scenarios were compared using LCA: (i) producing concentrated liquid feed by centrifugation (CC) of CRW with wastewater treatment and discharge of the supernatant, (ii) producing concentrated liquid feed by heating evaporation (HC) of CRW, (iii) producing dehydrated feed by dehydration (DH) of CRW, and (iv) wastewater treatment and discharge of nonconcentrated ricewashing water (WT). The functional unit (FU) was defined as 1 metric ton of rice washed for cooking or processing. Our results suggested that the energy consumptions of CC, HC, DH, and WT were 108, 322, 739, and 242 MJ per FU, respectively, and the amounts of GHG emissions from CC, HC, DH, and WT were 6.4, 15.8, 45.5, and 22.5 kg of CO2 equivalents per FU, respectively. When the produced feed prepared from CRW was assumed to be transported 200 km to farms, CC and HC still emitted smaller GHGs than the other scenarios, and CC consumed the smallest amount of energy among the scenarios. Th e present study indicates that liquid feed production from CRW by centrifugation has a remarkably reducedm environmental impact compared with the wastewater treatment and discharge of rice-washing water. © American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. Source

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