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Kumamoto-shi, Japan

Shokei College is a private university in Kumamoto, Kumamoto, Japan. The predecessor of the school, small girl's school, was founded in 1888, and it was chartered as a junior women's college in 1952. In 1975, Shokei University was established and the junior women's college was renamed to Shokei Junior College. In 2006, the two schools merged. Wikipedia.

In tomato production, salt stress is applied to improve the fruit quality. The change in sensory attributes and organoleptic compositions in salt-stressed tomato fruits have been extensively studied; however, little is known about their interaction with each other. Correlation network analysis provides a visual representation of biological systems and useful knowledge for metabolic data analysis of tomato fruits. The aim of this study is to identify the cultivar differences in sensory attributes and organoleptic compositions of tomato fruits grown under salt stress and to elucidate their interaction among different cultivars using correlation network analysis. Salt stress was applied by adding 100. mM NaCl to the nutrient solution. Fruits were harvested at red-ripe stage and used for the evaluation of sensory attributes such as sweetness, sourness, umami, and tomato like, and organoleptic compositions such as sugar, organic acid, amino acid, and sodium ion contents. Almost all of the sensory attributes and organoleptic compositions of the fruit were significantly high in salt-stressed cultivars compared to non-stressed cultivars, and significant differences were also observed among cultivars. The correlation network analysis of the control fruit showed that compared to other traits, sugar is one of the key traits for improvement of tomato fruit quality based on high connectivity and betweenness centrality. In contrast, a high degree of positive connectivity was not observed between organoleptic compositions and sensory attributes in the salt-stressed fruit network. These results indicate that the relationship between sensory attributes and organoleptic compositions in fruits were different between the control and salt-stressed cultivars, suggesting that the salt-stressed fruit may have a different circuit of relationship compared to control. Furthermore, based on the increase ratio (salt stress/control) network results, we suggest that the increased sugar, organic acid, and amino acid contents may have contributed to the salt stress-induced enhancement of sensory attributes. © 2011 Elsevier B.V. Source

Kawanobu S.,Minami Kyushu University | Yamaguchi M.,Minami Kyushu University | Zushi K.,Shokei College | Kondo K.,Tottori University | Matsuzoe N.,Prefectural University of Kumamoto
Journal of Food, Agriculture and Environment

The pigment composition of strawberry (Fragaria × ananassa Duch.) fruits mainly consists of anthocyanins, which are important components for fruit coloration and which have beneficial effects on human health. The anthocyanin content of the fruits of 48 strawberry cultivars and wild-type Fragaria vesca was analyzed. Cyanidin 3-glucoside (Cy3G), pelargonidin 3-glucoside (Pg3G) and pelargonidin 3-malonylglucoside (Pg3MG) were identified as major anthocyanins on the basis of the results of TLC, HPLC, and sugar and organic acid analyses, respectively. Cy3G and Pg3G were detected in each fruit that was sampled. In all the cultivars, Pg3G accounted for at least 50% of the total anthocyanin content, while Pg3MG was found in several cultivars. Source

Zushi K.,Shokei College | Matsuzoe N.,Prefectural University of Kumamoto
Journal of the Japanese Society for Horticultural Science

L-ascorbate (ASA; a reduced form of vitamin C) and glutathione (GSH) in tomato fruit participate in many diverse biological processes such as fruit ripening. In general, ASA and GSH are regulated by the reactive oxygen species scavenging system (antioxidant system). The tomato is a complex fruit composed of skin, pericarp walls, placenta, locular tissue, and seed tissues, and it is unclear whether the antioxidant contents and systems differ among these tissues. The aim of this study is to clarify tissue-specific changes in oxidative parameters, antioxidant contents, and antioxidant enzymes during fruit ripening in the tomato pericarp (skin, pericarp walls) and pulp (placenta, locular tissue, seeds). Lipid peroxidation and the H 2O 2 content remained constant during fruit ripening and were lower in the pulp than in the pericarp. In addition, ASA and GSH contents were the same in both these tissues when the fruits were picked at the red stage; however, changes in the superoxide dismutase, catalase, and ASA-GSH cycles, related to enzymes such as ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase, showed tissue-specific differences during ripening. These results indicate that ASA and GSH contents in both tissues are the same when fruits are picked at the red stage, but oxidative parameters and antioxidant systems show tissue-specific differences during fruit ripening. Furthermore, our results indicate that oxidative stress in the pulp was lower than that in the pericarp. This work therefore provides a basis for understanding the role of antioxidant systems during tomato fruit ripening. © 2012. Source

Uchida M.,Mizuki biotech. co. | Ishibashi H.,Shokei College | Yamamoto R.,Prefectural University of Kumamoto | Koyanagi A.,Mizuki biotech. co. | And 4 more authors.
Journal of Applied Toxicology

Although several previous studies have demonstrated the presence of equine estrogens in the aquatic environment, limited data are currently available on the endocrine-disrupting potentials in fish and the risks they pose to aquatic organisms. To investigate the interactions of major equine estrogens equilin (Eq) and equilenin (Eqn), as well as their metabolites 17α-dihydroequilin, 17β-dihydroequilin, 17α-dihydroequilenin and 17β-dihydroequilenin, with the estrogen receptor α (ERα) of medaka (Oryzias latipes), a three-dimensional model of the ligand-binding domain (LBD) of ERα was built in silico, and docking simulations were performed. The docking simulation analysis indicated that the interaction of 17β-dihydroequilenin with the ERα LBD is the most potent, followed by those of 17α-dihydroequilin and 17β-dihydroequilin, whereas those of Eq and Eqn were least potent. We further analyzed gene expression profiles in the livers of male medaka exposed to Eq and Eqn. A DNA microarray representing 6000 genes revealed that 24-h exposure to Eq and Eqn (100ng/L) upregulated the expression of 6 and 34 genes in the livers of males, respectively. Genes upregulated by Eq included the estrogenic biomarker genes vitellogenins and choriogenins, suggesting the estrogenic potential of Eq. In contrast, Eqn exposure upregulated several cancer-related genes, such as mediator complex subunit 16 and RAS oncogene family members, suggesting a carcinogenic potential for Eqn. These results suggest that equine estrogens may have not only endocrine-disrupting potentials via the ERα signaling pathway but also carcinogenic potency in male medaka. © 2015 John Wiley & Sons, Ltd. Source

Shigematsu Y.,Shokei College | Hatano Y.,Kyushu University | Kimura H.,Kyushu University
Public Health Nursing

Objective: The objective of this study was to develop and test a Partnership Development Process Assessment (PDPA) scale for content and construct validity and internal consistency reliability. This is needed to document and evaluate community health partnership development processes between public health nurses and community-based organizations in Japan. Design: The study was conducted in three phases. Ten semi-structured interviews were conducted to generate items for a new scale. Thirty items were generated and reviewed by an expert panel for content validity and item refinement. A national postal survey of public health nurses was conducted to determine the scale's internal structure, evaluate its reliability, and explore its construct and criterion validity. Measures: Validity and reliability testing of the PDPA scale using a content validity index and analysis of correlations with an existing scale were performed. Results: Twenty-six items were selected and grouped into four factors: activities to share roles to manage community health issues, platform activities to support partnerships, activities to evaluate partnership practices, and activities to share information regarding community health issues. After factor analysis, 23 items were retained. Conclusion: The PDPA scale is a valid and reliable instrument for public health nurses to assess partnership development activities. © 2015 Wiley Periodicals, Inc. Source

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