Kumamoto-shi, Japan
Kumamoto-shi, Japan

Shokei College is a private university in Kumamoto, Kumamoto, Japan. The predecessor of the school, small girl's school, was founded in 1888, and it was chartered as a junior women's college in 1952. In 1975, Shokei University was established and the junior women's college was renamed to Shokei Junior College. In 2006, the two schools merged. Wikipedia.

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PubMed | Shokei College, Seikai National Fisheries Research Institute and Prefectural University of Kumamoto
Type: | Journal: Chemosphere | Year: 2014

To evaluate trophic biomagnification of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in an estuary of the Ariake Sea, Japan, we measured concentrations of 209 PCB congeners and 28 PBDE congeners, and nitrogen stable isotope ((15)N) levels in living aquatic organisms. The trophic magnification factor (TMF) for PCBs (all 209 congeners) was 1.52, and TMFs for 58 PCB congeners ranged from 0.90 to 3.28. In contrast, TMF for PBDEs was 1.17, and TMFs for 7 PBDE congeners ranged from 0.46 to 1.66. TMFs of PCB and PBDE congeners in this study were lower than those in marine food webs, and were similar to those in a lake food web. However, although negative relationships were observed between TMF and log octanol-water partition coefficient (KOW) values among PCB congeners in this study (log KOW up to 7), positive relationships have been reported in several other studies. In the present estuary, PCB concentrations in sea bass may not reach a steady state because sea bass are migratory species. Therefore, TMFs of highly chlorinated congeners with high log KOW values take longer to reach the steady state and may not increase with increasing log KOW.


Uchida M.,Mizuki biotech Co. | Ishibashi H.,Shokei College | Yamamoto R.,Prefectural University of Kumamoto | Koyanagi A.,Mizuki biotech Co. | And 4 more authors.
Journal of Applied Toxicology | Year: 2015

Although several previous studies have demonstrated the presence of equine estrogens in the aquatic environment, limited data are currently available on the endocrine-disrupting potentials in fish and the risks they pose to aquatic organisms. To investigate the interactions of major equine estrogens equilin (Eq) and equilenin (Eqn), as well as their metabolites 17α-dihydroequilin, 17β-dihydroequilin, 17α-dihydroequilenin and 17β-dihydroequilenin, with the estrogen receptor α (ERα) of medaka (Oryzias latipes), a three-dimensional model of the ligand-binding domain (LBD) of ERα was built in silico, and docking simulations were performed. The docking simulation analysis indicated that the interaction of 17β-dihydroequilenin with the ERα LBD is the most potent, followed by those of 17α-dihydroequilin and 17β-dihydroequilin, whereas those of Eq and Eqn were least potent. We further analyzed gene expression profiles in the livers of male medaka exposed to Eq and Eqn. A DNA microarray representing 6000 genes revealed that 24-h exposure to Eq and Eqn (100ng/L) upregulated the expression of 6 and 34 genes in the livers of males, respectively. Genes upregulated by Eq included the estrogenic biomarker genes vitellogenins and choriogenins, suggesting the estrogenic potential of Eq. In contrast, Eqn exposure upregulated several cancer-related genes, such as mediator complex subunit 16 and RAS oncogene family members, suggesting a carcinogenic potential for Eqn. These results suggest that equine estrogens may have not only endocrine-disrupting potentials via the ERα signaling pathway but also carcinogenic potency in male medaka. © 2015 John Wiley & Sons, Ltd.


PubMed | Ariake National College of Technology, Shokei College and Prefectural University of Kumamoto
Type: Journal Article | Journal: Journal of applied toxicology : JAT | Year: 2015

Lithium (Li) has been widely used to treat bipolar disorder, and industrial use of Li has been increasing; thus, environmental pollution and ecological impacts of Li have become a concern. This study was conducted to clarify the potential biological effects of LiCl and Li(2)CO(3) on a nematode, Caenorhabditis elegans as a model system for evaluating soil contaminated with Li. Exposure of C. elegans to LiCl and Li(2)CO(3) decreased growth/maturation and reproduction. The lowest observed effect concentrations for growth, maturation and reproduction were 1250, 313 and 10 000m, respectively, for LiCl and 750, 750 and 3000m, respectively, for Li(2)CO(3). We also investigated the physiological function of LiCl and LiCO(3) in C. elegans using DNA microarray analysis as an eco-toxicogenomic approach. Among approximately 300 unique genes, including metabolic genes, the exposure to 78m LiCl downregulated the expression of 36 cytochrome P450, 16 ABC transporter, 10 glutathione S-transferase, 16 lipid metabolism and two vitellogenin genes. On the other hand, exposure to 375m Li(2)CO(3) downregulated the expression of 11 cytochrome P450, 13 ABC transporter, 13 lipid metabolism and one vitellogenin genes. No gene was upregulated by LiCl or Li(2)CO(3). These results suggest that LiCl and Li(2)CO(3) potentially affect the biological and physiological function in C. elegans associated with alteration of the gene expression such as metabolic genes. Our data also provide experimental support for the utility of toxicogenomics by integrating gene expression profiling into a toxicological study of an environmentally important organism such as C. elegans.


Zushi K.,Shokei College | Matsuzoe N.,Prefectural University of Kumamoto
Scientia Horticulturae | Year: 2011

In tomato production, salt stress is applied to improve the fruit quality. The change in sensory attributes and organoleptic compositions in salt-stressed tomato fruits have been extensively studied; however, little is known about their interaction with each other. Correlation network analysis provides a visual representation of biological systems and useful knowledge for metabolic data analysis of tomato fruits. The aim of this study is to identify the cultivar differences in sensory attributes and organoleptic compositions of tomato fruits grown under salt stress and to elucidate their interaction among different cultivars using correlation network analysis. Salt stress was applied by adding 100. mM NaCl to the nutrient solution. Fruits were harvested at red-ripe stage and used for the evaluation of sensory attributes such as sweetness, sourness, umami, and tomato like, and organoleptic compositions such as sugar, organic acid, amino acid, and sodium ion contents. Almost all of the sensory attributes and organoleptic compositions of the fruit were significantly high in salt-stressed cultivars compared to non-stressed cultivars, and significant differences were also observed among cultivars. The correlation network analysis of the control fruit showed that compared to other traits, sugar is one of the key traits for improvement of tomato fruit quality based on high connectivity and betweenness centrality. In contrast, a high degree of positive connectivity was not observed between organoleptic compositions and sensory attributes in the salt-stressed fruit network. These results indicate that the relationship between sensory attributes and organoleptic compositions in fruits were different between the control and salt-stressed cultivars, suggesting that the salt-stressed fruit may have a different circuit of relationship compared to control. Furthermore, based on the increase ratio (salt stress/control) network results, we suggest that the increased sugar, organic acid, and amino acid contents may have contributed to the salt stress-induced enhancement of sensory attributes. © 2011 Elsevier B.V.


Shigematsu Y.,Shokei College | Hatano Y.,Kyushu University | Kimura H.,Kyushu University
Public Health Nursing | Year: 2015

Objective: The objective of this study was to develop and test a Partnership Development Process Assessment (PDPA) scale for content and construct validity and internal consistency reliability. This is needed to document and evaluate community health partnership development processes between public health nurses and community-based organizations in Japan. Design: The study was conducted in three phases. Ten semi-structured interviews were conducted to generate items for a new scale. Thirty items were generated and reviewed by an expert panel for content validity and item refinement. A national postal survey of public health nurses was conducted to determine the scale's internal structure, evaluate its reliability, and explore its construct and criterion validity. Measures: Validity and reliability testing of the PDPA scale using a content validity index and analysis of correlations with an existing scale were performed. Results: Twenty-six items were selected and grouped into four factors: activities to share roles to manage community health issues, platform activities to support partnerships, activities to evaluate partnership practices, and activities to share information regarding community health issues. After factor analysis, 23 items were retained. Conclusion: The PDPA scale is a valid and reliable instrument for public health nurses to assess partnership development activities. © 2015 Wiley Periodicals, Inc.


Zushi K.,Shokei College | Kajiwara S.,Prefectural University of Kumamoto | Matsuzoe N.,Prefectural University of Kumamoto
Scientia Horticulturae | Year: 2012

In tomato production, extreme temperatures such as heat and chilling are major factors limiting growth and productivity. Recently, the chlorophyll a fluorescence OJIP transient (OJIP transient) has been used as an effective tool for studying damage to and activity of the electron transport chain in the photosynthetic apparatus under various environmental stresses. The aim of this study was to evaluate the validity of the OJIP transient as a stress indicator and to characterize the effect of heat and chilling stress on the photosynthetic apparatus in tomato leaf and fruit. Detached leaves and fruits were incubated at 25. °C (control), 40. °C (heat stress), and 4. °C (chilling stress); OJIP transients were measured after exposure to stress for 1. h and 24. h, and several parameters were calculated according to the JIP-test. After exposure to stress for 1. h and 24. h, the OJIP curves and the JIP parameters clearly revealed differences between stress types and between tissue types. In addition, the JIP parameters and the energy pipeline model indicated that heat stress had a greater influence on the photosystem (PS) II electron transport chain than chilling stress, and that changes were greater in the fruit than in the leaf. Furthermore, the PS I electron transport chains of leaf and fruit appeared to be more heat resistant than those in PS II. Our results indicate that, in tomato leaf and fruit, OJIP transients and calculated JIP parameters can be used as sensitive methods for measuring the heat and chilling stress damage to the photosynthetic apparatus, and to identify the action sites of temperature stress. © 2012 Elsevier B.V.


Honda T.,Prefectural University of Kumamoto | Zushi K.,Shokei College | Matsuzqe N.,Prefectural University of Kumamoto
Environmental Control in Biology | Year: 2012

Eggplant (Solarium melongenaJ..) fruit contains several anthocyanins, and their levels are well known to fluctuate depending on the light condition. In eggplant, although two major anlhocyanins. nasunin and tulipanin, have been isolated from the fruit the inheritance mode of their light-dependent formation is not clear. In this study, lo clarify the genetic characteristics of anlhocyanin production and photosensitivity in eggplant fruits, we investigated the segregation and photosensitivity of anthocyanin in the F-, and F? hybrids of 'Kiimarnoto-naga' (Japanese eggplant containing nasunin) and "Black Beauty1 (.American eggplant containing tulipanin) grown under normal or dark conditions. The major anthocyanin of the fruit was nasunin. In the V fruit the anlhocyanins were present in a ratio of 3:1 (nasunin: tulipanin). In addition, based on the results obtained under dark conditions, the genetic characteristic of photosensitivity in the major anthocyanins was segregated into the ratio of 9:3:3:1 (nasunin of the non-photosensitive type : nasunin of the photosensitive type: tulipanin of the non-photosensitive type: tulipanin of the photosensitive type). These results reveal that the inheritance of major anthocyanins (nasunin and tulipanin) and that of the photosensitivity (photosensitive type and non-photosensitive type) of anthocyanin production do not interact but are independent.


Zushi K.,Shokei College | Matsuzoe N.,Prefectural University of Kumamoto
Journal of the Japanese Society for Horticultural Science | Year: 2012

L-ascorbate (ASA; a reduced form of vitamin C) and glutathione (GSH) in tomato fruit participate in many diverse biological processes such as fruit ripening. In general, ASA and GSH are regulated by the reactive oxygen species scavenging system (antioxidant system). The tomato is a complex fruit composed of skin, pericarp walls, placenta, locular tissue, and seed tissues, and it is unclear whether the antioxidant contents and systems differ among these tissues. The aim of this study is to clarify tissue-specific changes in oxidative parameters, antioxidant contents, and antioxidant enzymes during fruit ripening in the tomato pericarp (skin, pericarp walls) and pulp (placenta, locular tissue, seeds). Lipid peroxidation and the H 2O 2 content remained constant during fruit ripening and were lower in the pulp than in the pericarp. In addition, ASA and GSH contents were the same in both these tissues when the fruits were picked at the red stage; however, changes in the superoxide dismutase, catalase, and ASA-GSH cycles, related to enzymes such as ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase, showed tissue-specific differences during ripening. These results indicate that ASA and GSH contents in both tissues are the same when fruits are picked at the red stage, but oxidative parameters and antioxidant systems show tissue-specific differences during fruit ripening. Furthermore, our results indicate that oxidative stress in the pulp was lower than that in the pericarp. This work therefore provides a basis for understanding the role of antioxidant systems during tomato fruit ripening. © 2012.


Shinotsuka T.,Shokei College
Journal of the Japan Research Association for Textile End-Uses | Year: 2013

This study explores the concept of systematic consumer citizenship education for preschoolers at home by surveying previous studies on consumer and clothing behaviors and by examining the relation between clothing behavior and consumer citizenship. The study on the consumer behaviors of parents and preschoolers has begun since the 1980s and the study on their clothing behaviors has begun in the 1990s. However, actual teaching materials that could be used to provide consumer education to preschoolers have just been developed only recently. The examination of the relation between clothing behavior and consumer citizenship has provided insights for the development of teaching systems that could be used in consumer education. Through these systems, preschoolers can attain decision making, resource management, and citizen participation skills.


PubMed | Shokei College and Kyushu University
Type: Journal Article | Journal: Public health nursing (Boston, Mass.) | Year: 2015

The objective of this study was to develop and test a Partnership Development Process Assessment (PDPA) scale for content and construct validity and internal consistency reliability. This is needed to document and evaluate community health partnership development processes between public health nurses and community-based organizations in Japan.The study was conducted in three phases. Ten semi-structured interviews were conducted to generate items for a new scale. Thirty items were generated and reviewed by anexpert panel for content validity and item refinement. A national postal survey of public health nurses was conducted to determine the scales internal structure, evaluate its reliability, and explore its construct and criterion validity.Validity and reliability testing of thePDPA scale using a content validity index and analysis of correlations with an existing scalewere performed.Twenty-six items were selected and grouped into four factors: activities to share roles to manage community health issues, platform activities to support partnerships, activities to evaluate partnership practices, and activities to share information regarding community health issues. After factor analysis, 23 items were retained.The PDPA scale is a valid and reliable instrument for public health nurses to assess partnership development activities.

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